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1. 发明申请

US20080248535A1 RAPID ONE-STEP REVERSE TRANSCRIPTASE PCR 有权
标题翻译：快速一步反向转录PCR
公开(公告)号：US20080248535A1
公开(公告)日：2008-10-09
申请号：US12061700
申请日：2008-04-03
申请人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
发明人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12Q2527/113 , C12Q2521/107
摘要： The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
摘要翻译：本发明涉及一种用于进行扩增靶RNA的一步RT-PCR的方法，包括步骤（i）提供假定含有所述靶RNA的样品（ii）加入包含所需试剂的反应混合物将所述靶RNA逆转录入cDNA并扩增所述cDNA的至少一部分（iii）在20℃至65℃的温度下将所述样品温育0秒至40秒的时间间隔，和（iv ）使所述样品经受热循环方案的多个循环，其中所述样品的温度在37℃至72℃的至少第一温度和85℃至100℃的第二温度之间变化。

2. 发明授权

US08119353B2 Rapid one-step reverse transcriptase PCR 有权
标题翻译：快速一步逆转录酶PCR
公开(公告)号：US08119353B2
公开(公告)日：2012-02-21
申请号：US12061700
申请日：2008-04-03
申请人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
发明人： Waltraud Ankenbauer , Ursula Grepl , Rita Haerteis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6846 , C12Q2527/113 , C12Q2521/107
摘要： The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
摘要翻译：本发明涉及一种用于进行扩增靶RNA的一步RT-PCR的方法，包括步骤（i）提供假定含有所述靶RNA的样品（ii）加入包含所需试剂的反应混合物将所述靶RNA逆转录入cDNA并扩增所述cDNA的至少一部分（iii）在20℃至65℃的温度下将所述样品温育0秒至40秒的时间间隔，和（iv ）使所述样品经受热循环方案的多个循环，其中所述样品的温度在37℃至72℃的至少第一温度和85℃至100℃的第二温度之间变化。

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