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1. 发明授权

US07425423B1 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 失效
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US07425423B1
公开(公告)日：2008-09-16
申请号：US09269860
申请日：1997-10-01
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12 , C12Q1/48
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定酶源自嗜热古细菌热球菌。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

2. 发明申请

US20090093043A1 THERMOSTABLE NUCLEIC ACID POLYMERASE FROM THERMOCOCCUS GORGONARIUS 失效
标题翻译：热稳定性核酸聚合酶
公开(公告)号：US20090093043A1
公开(公告)日：2009-04-09
申请号：US12135994
申请日：2008-06-09
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

3. 发明授权

US06468775B1 Thermostable DNA polymerase from carboxydothermus hydrogenoformans 有权
标题翻译：来自羧酸热霉菌的热稳定DNA聚合酶
公开(公告)号：US06468775B1
公开(公告)日：2002-10-22
申请号：US09269861
申请日：1999-11-22
申请人： Waltraud Ankenbauer , Ursula Markau , Vitaly Svetlichny , Gudrun Schmitz-Agheguian , Astrid Reiser , Bernhard Angerer , Christine Ebenbichler , Frank Laue , Elizaveta Bonch-Osmolovskaya
发明人： Waltraud Ankenbauer , Ursula Markau , Vitaly Svetlichny , Gudrun Schmitz-Agheguian , Astrid Reiser , Bernhard Angerer , Christine Ebenbichler , Frank Laue , Elizaveta Bonch-Osmolovskaya
IPC分类号： C12N912
CPC分类号： C12N9/1252
摘要： A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.
摘要翻译：提供来自嗜热菌的DNA聚合酶。 DNA聚合酶显示镁离子依赖的逆转录酶活性和3'-5'核酸外切酶活性。本发明还包括能够产生酶的重组质粒和转化的宿主细胞。该酶被分类为EC 2.7.7.7。DNA，DNA核苷酸转移酶DNA指导型。

4. 发明授权

US08008054B2 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 有权
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US08008054B2
公开(公告)日：2011-08-30
申请号：US12793602
申请日：2010-06-03
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

5. 发明申请

US20110020898A1 Thermostable Nucleic Acid Polymerase From Thermococcus Gorgonarius 有权
标题翻译：热稳定性核糖核酸聚合酶
公开(公告)号：US20110020898A1
公开(公告)日：2011-01-27
申请号：US12793602
申请日：2010-06-03
申请人： Waltraud ANKENBAUER , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud ANKENBAUER , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

6. 发明授权

US07759107B2 Thermostable nucleic acid polymerase from Thermococcus gorgonarius 失效
标题翻译：来自Thermococcus gorgonarius的热稳定性核酸聚合酶
公开(公告)号：US07759107B2
公开(公告)日：2010-07-20
申请号：US12135994
申请日：2008-06-09
申请人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
发明人： Waltraud Ankenbauer , Vitaly Svetlichny , Elizaveta Bonch-Osmolovskaya , Christine Ebenbichler , Bernhard Angerer , Gudrun Schmitz-Agheguian , Frank Laue
IPC分类号： C12N9/12 , C12N1/20 , C12Q1/48
CPC分类号： C12N9/1252
摘要： A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
摘要翻译：纯化的热稳定性酶源自嗜热古细菌Thermococcus gorgonarius。该酶可以是天然的或重组的，在稳定剂存在下在95℃孵育2小时后保留其活性的约90％，并且具有3'-5'校对外切核酸酶活性。热稳定的DNA聚合酶可用于许多重组DNA技术，特别是通过聚合酶链反应（PCR）进行的核酸扩增。

7. 发明授权

US06692932B1 Thermostable DNA polymerase from anaerocellum thermophilum 有权
标题翻译：热嗜热嗜热厌氧细胞的DNA聚合酶
公开(公告)号：US06692932B1
公开(公告)日：2004-02-17
申请号：US09269858
申请日：1999-06-10
申请人： Waltraud Ankenbauer , Gudrun Schmitz-Agheguian , Elizaveta Bonch-Osmolovskaya , Vitaly Svetlichny , Ursula Markau , Bernhard Angerer , Astrid Reiser
发明人： Waltraud Ankenbauer , Gudrun Schmitz-Agheguian , Elizaveta Bonch-Osmolovskaya , Vitaly Svetlichny , Ursula Markau , Bernhard Angerer , Astrid Reiser
IPC分类号： C12Q148
CPC分类号： C12N9/1252
摘要： A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophiluml. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.
摘要翻译：提供了一种来自微生物嗜热厌氧细胞嗜热细菌的热稳定酶。该酶具有96至100kDa的分子量，在镁离子存在下显示DNA聚合酶活性和逆转录酶活性。该酶可以是天然的或重组的，并且可以在DNA或RNA的温度循环聚合酶链式反应中用选定的引物和三磷酸核苷作为模板，使用或不含有另外的DNA聚合酶作为酶混合物。

8. 发明申请

US20050019789A1 Thermostable DNA polymerase from anaerocellum thermophilum 审中-公开
标题翻译：热嗜热嗜热厌氧细胞的DNA聚合酶
公开(公告)号：US20050019789A1
公开(公告)日：2005-01-27
申请号：US10770989
申请日：2004-02-02
申请人： Waltraud Ankenbauer , Gudrun Schmitz-Agheguian , Elizaveta Bonch-Osmolovskaya , Vitaly Svetlichny , Ursula Markau , Bernhard Angerer , Astrid Reiser
发明人： Waltraud Ankenbauer , Gudrun Schmitz-Agheguian , Elizaveta Bonch-Osmolovskaya , Vitaly Svetlichny , Ursula Markau , Bernhard Angerer , Astrid Reiser
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/12 , C12N15/52 , C12N15/70 , C12P21/00 , C12Q1/68 , C12R1/01 , C07H21/04 , C12N9/22 , C12N15/74
CPC分类号： C12N9/1252
摘要： A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophilum. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.
摘要翻译：提供一种源自微生物嗜热厌氧细菌的热稳定酶。该酶具有96至100kDa的分子量，在镁离子存在下显示DNA聚合酶活性和逆转录酶活性。该酶可以是天然的或重组的，并且可以在DNA或RNA的温度循环聚合酶链式反应中用选定的引物和三磷酸核苷作为模板，使用或不含有另外的DNA聚合酶作为酶混合物。

9. 发明授权

US5134068A Type II restriction endonucleae MCRI 失效
标题翻译：第二类限制性内源性MCRI
公开(公告)号：US5134068A
公开(公告)日：1992-07-28
申请号：US615438
申请日：1990-11-16
申请人： Klaus Kaluza , Hans J. Hoeltke , Michael Jarsch , Gudrun Schmitz-Agheguian , Christoph Kessler
发明人： Klaus Kaluza , Hans J. Hoeltke , Michael Jarsch , Gudrun Schmitz-Agheguian , Christoph Kessler
IPC分类号： C12N9/16 , C12N9/22 , C12R1/265
CPC分类号： C12N9/22
摘要： Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:5'-CGRYCG-3'and its complementary sequence.
摘要翻译：公开了II型限制性内切核酸酶McrI。该核酸内切酶具有识别序列：其中R是G或A，Y是C或T，以及由箭头表示的切割位点。核酸内切酶优选从微球菌属的生物获得。可用于识别和切割双链DNA序列：5'-CGRYCG-3'及其互补序列。

10. 发明申请

US20060099596A1 Multiplex assay detection of pathogenic organisms 审中-公开
标题翻译：病原生物多重检测检测
公开(公告)号：US20060099596A1
公开(公告)日：2006-05-11
申请号：US10534915
申请日：2003-12-02
申请人： Gerd Haberhausen , Thomas Emrich , Gregor Sagner , Martin Moczko , Gudrun Schmitz-Agheguian
发明人： Gerd Haberhausen , Thomas Emrich , Gregor Sagner , Martin Moczko , Gudrun Schmitz-Agheguian
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6851
摘要： The present invention is directed to a method for identification of a pathogenic organism from a predetermined group of pathogens, comprising (i) isolating a clinical sample containing at least partially purified nucleic acid, (ii) subjecting at least a first aliquot of said clinical specimen to at least one amplification and detection reaction in one reaction vessel comprising (iia) an amplification step using at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from several or all members of said predetermined group of pathogens, (iib) a detection step using at least 2, 3 or multiple hybridization reagents, said reagents together being capable of specifically detecting a pre-selected nucleic acid sequence region from all members of said group of pathogens, said detection step (iib) comprising steps monitoring hybridization of each of said hybridization reagents at a pre-selected temperature, said hybridization being indicative for at least the genus of said pathogen present in the sample, and monitoring temperature dependence of hybridization, said temperature dependence being indicative for at least the species of said pathogen, and (iii) determining whether said amplification and detection reaction is indicative for the presence of a specific member of said pre-selected group of pathogens.
摘要翻译：本发明涉及从预定病原体组中鉴定病原生物的方法，其包括（i）分离含有至少部分纯化的核酸的临床样品，（ii）对所述临床标本的至少第一等分试样至少在一个反应容器中进行扩增和检测反应，其包括（i）扩增步骤，使用至少第一组扩增引物，所述第一组扩增引物能够扩增来自所述预定病原体组的数个或全部成员的预选核酸序列区（ iib）使用至少2,3或多个杂交试剂的检测步骤，所述试剂一起能够特异性检测来自所述病原体组的所有成员的预先选择的核酸序列区，所述检测步骤（iib）包括监测步骤每个所述杂交试剂在预选温度下进行杂交，所述杂交是指示性的至少存在于样品中的所述病原体的属和监测杂交的温度依赖性，所述温度依赖性指示至少所述病原体的种类，和（iii）确定所述扩增和检测反应是否表明存在的所述预先选择的病原体组的特定成员。

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