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1. 发明申请

US20090068750A1 Method of Determining Iron Concentration 失效
标题翻译：铁浓度测定方法
公开(公告)号：US20090068750A1
公开(公告)日：2009-03-12
申请号：US11918151
申请日：2006-05-09
申请人： Wataru Akahane , Haruhisa Ijiri , Toshiro Hanada
发明人： Wataru Akahane , Haruhisa Ijiri , Toshiro Hanada
IPC分类号： G01N33/20
CPC分类号： G01N33/84 , G01N21/78 , G01N21/80 , G01N31/22 , G01N33/52
摘要： The present invention discloses a method of measuring iron concentration in a sample, characterized by bringing the iron contained in the sample into contact with a metallochromic indicator for iron in the presence of lithium ion and determining the iron concentration based on the degree of resultant coloring, a reagent and a kit using therefor.By performing the method of measuring iron concentration of the present invention, the conventional problem that the measured value is lower as compared with that obtained by the International Standard Method is solved; a complicated procedures such as protein removal treatment or the like are not required; the measurement of iron concentration with an automated analyzer is enabled; and more accurate measurement becomes possible, since the measured value obtained by the present method is very close to that obtained by the standard addition method according to the International Standard Method,
摘要翻译：本发明公开了一种测定样品中铁浓度的方法，其特征在于，在锂离子的存在下使包含在样品中的铁与铁的金属致变色指示剂接触，并且基于所得着色程度确定铁浓度，试剂和使用它的试剂盒。通过进行本发明的铁浓度测定方法，解决了与国际标准方法相比测定值低的常规问题，不需要诸如蛋白质去除处理等复杂的程序; 使用自动分析仪测量铁浓度; 并且由于通过本方法得到的测定值与通过国际标准法的标准添加方法得到的测定值非常接近，所以能够进行更精确的测定，

2. 发明授权

US5814472A Measurement of LDL-cholesterol 失效
标题翻译： LDL胆固醇测定
公开(公告)号：US5814472A
公开(公告)日：1998-09-29
申请号：US943008
申请日：1997-10-02
申请人： Yutaka Miki , Nobuko Imajo , Isao Koyama , Toshiro Hanada
发明人： Yutaka Miki , Nobuko Imajo , Isao Koyama , Toshiro Hanada
IPC分类号： C12Q1/60 , G01N33/92 , C12Q1/00 , C12Q1/32 , C12Q1/44
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044
摘要： Cholesterol in low density lipoproteins can be measured specifically and precisely by optically measuring a reaction product of a living sample with cholesterol oxidase or cholesterol dehydrogenase in the presence of an amphoteric surfactant. In addition to the above processes for measurement, the present invention provides reagent compositions and kits for measuring cholesterol in low density lipoprotein.
摘要翻译：低密度脂蛋白中的胆固醇可以通过在两性表面活性剂的存在下光活化样品与胆固醇氧化酶或胆固醇脱氢酶的反应产物进行光学测量而被特异地和精确地测量。除了上述测量方法之外，本发明还提供了用于测量低密度脂蛋白胆固醇的试剂组合物和试剂盒。

3. 发明授权

US4695540A Quantitative determination of substrate treated with oxidase 失效
标题翻译：用氧化酶处理的底物的定量测定
公开(公告)号：US4695540A
公开(公告)日：1987-09-22
申请号：US516271
申请日：1983-07-22
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： C12Q1/26 , C12Q1/54 , C12Q1/60 , C12Q1/62 , C12Q1/28
CPC分类号： C12Q1/26 , C12Q1/54 , C12Q1/60 , C12Q1/62 , Y10S435/81
摘要： Cholesterol in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with cholesterol oxidase. Similarly, a substrate such as glucose, etc., can be determined quantitatively by measuring the generated superoxide ion colorimetrically, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b') iron complex of porphyrin or ion chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
摘要翻译：通过测量通过用胆固醇氧化酶处理样品产生的比色超氧化物离子，可以定量测定样品中的胆固醇。类似地，可以通过比色测定生成的超氧化物离子，通过使用包含（a）氧化酶（b'）的试剂组合物进行的所述氧化酶处理和测量所产生的超氧化物离子来定量测定诸如葡萄糖等的底物，）卟啉的络合物或络合物的离子螯合物，（c）胺和/或苯酚，（d）硫醇化合物，（e）待还原的着色剂，以及必要时（f）螯合剂。

4. 发明授权

US4622296A Process for measuring activity of dehydrogenase employing a reaction stopper 失效
标题翻译：使用反应塞测定脱氢酶的活性的方法
公开(公告)号：US4622296A
公开(公告)日：1986-11-11
申请号：US686817
申请日：1984-12-27
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： G01N33/50 , C12Q1/32
CPC分类号： C12Q1/32
摘要： In a process for measuring the activity of a dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazolium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used as a reaction stopper, resulting in enhancement of coloring, stability of the produced color and prevention of the produced formazan compound from staining the laboratoryware.
摘要翻译：在使用NAD或NADP作为辅酶和四唑鎓盐作为着色试剂时，在脱氢酶存在下测定脱氢酶的活性或底物反应量的方法中，至少一种选自十二烷基硫酸盐，癸基硫酸盐，十二烷基苯磺酸及其盐用作反应停止剂，导致着色增强，产生颜色的稳定性和防止生成的甲an化合物染色实验室用品。

5. 发明授权

US4613465A Triphenyl methane derivatives and method of quantitatively measuring an oxidative substance 失效
标题翻译：三苯甲烷衍生物和定量测定氧化物质的方法
公开(公告)号：US4613465A
公开(公告)日：1986-09-23
申请号：US649477
申请日：1984-09-11
申请人： Kazuhiko Yamanishi , Akinori Shintani , Satoru Okajima , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Akinori Shintani , Satoru Okajima , Toshiro Hanada
IPC分类号： B41M5/136 , C09B11/00 , C09B11/12 , C09B11/14 , C12Q1/28 , C09B11/10
CPC分类号： B41M5/1366 , C09B11/12 , C12Q1/28 , C12Q2326/40 , Y10S436/904 , Y10T436/206664
摘要： Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent lower alkyl groups, and X.sub.1 and X.sub.2 both represent --O(CH.sub.2).sub.n SO.sub.3 M in which M is a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, and n is an integer of 2-4, or either of X.sub.1 and X.sub.2 represents --O(CH.sub.2).sub.n SO.sub.3 M in whch M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side. Disclosed is also a method of quantitatively measuring an oxidative substance, particularly, hydrogen peroxide, by using this color test reagent.
摘要翻译：本文公开了一种新颖的化合物，其在中性范围内对水的溶解性优异，在水溶液状态下长时间稳定，着色敏感性高，稳定的显色性，并且由通式（ I）：其中R 1，R 2，R 3和R 4可以相同或不同，表示低级烷基，X 1和X 2均表示-O（CH 2）n SO 3 M，其中M 是氢原子，碱金属离子或NH 4 +，n是2-4的整数，或者X 1和X 2中的任一个表示-O（CH 2）n SO 3 M，m和n与上述相同，而另一个表示氢原子。该试剂由于其在长波长侧具有吸收的颜色，几乎不受血清成分如血红蛋白，胆红素等的影响。还公开了通过使用该颜色试验剂定量测定氧化物质，特别是过氧化氢的方法。

6. 发明授权

US4592996A Process for determining reduced form coenzymes 失效
标题翻译：确定还原型辅酶的方法
公开(公告)号：US4592996A
公开(公告)日：1986-06-03
申请号：US608681
申请日：1984-05-10
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： C12Q1/00 , C12Q1/28 , C12Q1/32 , C12Q1/60 , C12N9/96
CPC分类号： C12Q1/28 , C12Q1/008 , C12Q1/60
摘要： A reduced form coenzyme can be determined by acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide and measuring the amount of hydrogen peroxide colorimetrically.
摘要翻译：还原型辅酶可以通过在金属离子存在下在还原型辅酶上起作用过氧化物酶来产生过氧化氢并以比色测量过氧化氢的量来测定。

7. 发明授权

US4295853A Method for measuring peroxidic substances 失效
标题翻译：过氧化物质测定方法
公开(公告)号：US4295853A
公开(公告)日：1981-10-20
申请号：US974148
申请日：1978-12-29
申请人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
发明人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
IPC分类号： C07D231/48 , G01N31/22 , G01N33/52 , G01N33/92
CPC分类号： G01N33/52 , C07D231/48 , G01N31/228
摘要： Peroxidic substances in a sample, e.g. one or more peroxidic substances in fats and oils or in an organic material, lipoperoxides in a living sample, etc. can be determined, directly in a sample or after extracting the lipoperoxides from a living sample with a solvent, by contacting the peroxidic substances with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color, and measuring degree of coloring colorimetrically.
摘要翻译：样品中的过氧化物质，例如脂肪和油或有机材料中的一种或多种过氧化物质，活体样品中的脂肪过氧化物等可以直接在样品中或在用溶剂从活体样品中提取脂肪过氧化物之后，通过使过氧化物质与在由能够具有两个或多个化合价产生颜色的金属制成的金属化合物存在下的可氧化着色试剂，并以比色测定着色程度。

8. 发明授权

US07785892B2 Method of determining iron concentration 失效
标题翻译：铁浓度测定方法
公开(公告)号：US07785892B2
公开(公告)日：2010-08-31
申请号：US11918151
申请日：2006-05-09
申请人： Wataru Akahane , Haruhisa Ijiri , Toshiro Hanada
发明人： Wataru Akahane , Haruhisa Ijiri , Toshiro Hanada
IPC分类号： G01N33/20 , G01N33/48
CPC分类号： G01N33/84 , G01N21/78 , G01N21/80 , G01N31/22 , G01N33/52
摘要： The present invention discloses a method of measuring iron concentration in a sample. The method includes bringing iron contained in the sample into contact with a metallochromic indicator for iron in the presence of lithium ion and determining the iron concentration based on the degree of resultant coloring. The present disclosure further includes a reagent and a kit using therefor.
摘要翻译：本发明公开了一种测量样品中铁浓度的方法。该方法包括在锂离子的存在下使包含在样品中的铁与铁的金属致变色指示剂接触，并且基于所得着色程度确定铁浓度。本公开还包括试剂和使用它的试剂盒。

9. 发明授权

US6162607A Method for measuring the amount of constituent contained in specific lipoprotein 失效
标题翻译：用于测量特定脂蛋白中含有的成分的量的方法
公开(公告)号：US6162607A
公开(公告)日：2000-12-19
申请号：US684928
申请日：1996-07-19
申请人： Yutaka Miki , Toshiro Hanada , Kiyoko Tanaka
发明人： Yutaka Miki , Toshiro Hanada , Kiyoko Tanaka
IPC分类号： G01N21/59 , G01N33/92 , G01N33/503
CPC分类号： G01N33/92 , G01N21/59 , G01N2800/044 , Y10S435/805 , Y10S435/962 , Y10S436/805 , Y10S436/81 , Y10S436/811 , Y10S436/824 , Y10S436/825
摘要： There is provided a method and kit for measuring the amount of an objective constituent contained in a specific lipoprotein in a biological sample such as serum and plasma, specifically for measuring the amount of cholesterol contained in high density lipoprotein, which can be applicable to clinical tests.
摘要翻译：提供了用于测量生物样品如血清和血浆中特定脂蛋白中包含的客观成分的量的方法和试剂盒，特别是用于测量高密度脂蛋白中含有的胆固醇的量，其可应用于临床试验。

10. 发明授权

US5384248A Process for measuring an analyte with an oxidase and an oxidizable color reagent and surfactants 失效
标题翻译：用氧化酶和可氧化着色试剂和表面活性剂测量分析物的方法
公开(公告)号：US5384248A
公开(公告)日：1995-01-24
申请号：US228002
申请日：1994-04-15
申请人： Yoshitsugu Sakata , Toshiro Hanada , Ryosuke Matsuda , Yoshiyuki Matsuda
发明人： Yoshitsugu Sakata , Toshiro Hanada , Ryosuke Matsuda , Yoshiyuki Matsuda
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/34 , C12Q1/44 , C12Q1/46 , C12Q1/54 , C12Q1/60 , C12Q1/61 , C12Q1/62 , C12Q1/36
CPC分类号： C12Q1/28
摘要： A substrate or an enzymatic activity in a body fluid can be measured accurately without influences of interfering substances such as bilirubin by reacting an oxidase corresponding to an analyte with the analyte or an oxidase corresponding to a substance produced by enzymatic reaction with the substance in a measuring system containing one or more cationic and/or amphoteric surfactants, followed by optical measurement of hydrogen peroxide produced by the reaction.
摘要翻译：体液中的底物或酶活性可以通过将与分析物相应的氧化酶与分析物相反应的氧化酶或对应于通过与物质的酶反应产生的物质的氧化酶在测量中与物质反应而精确地测量而不受干扰物质例如胆红素的影响含有一种或多种阳离子和/或两性表面活性剂的体系，然后光学测量由反应产生的过氧化氢。

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