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1. 发明授权

US4295853A Method for measuring peroxidic substances 失效
标题翻译：过氧化物质测定方法
公开(公告)号：US4295853A
公开(公告)日：1981-10-20
申请号：US974148
申请日：1978-12-29
申请人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
发明人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
IPC分类号： C07D231/48 , G01N31/22 , G01N33/52 , G01N33/92
CPC分类号： G01N33/52 , C07D231/48 , G01N31/228
摘要： Peroxidic substances in a sample, e.g. one or more peroxidic substances in fats and oils or in an organic material, lipoperoxides in a living sample, etc. can be determined, directly in a sample or after extracting the lipoperoxides from a living sample with a solvent, by contacting the peroxidic substances with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color, and measuring degree of coloring colorimetrically.
摘要翻译：样品中的过氧化物质，例如脂肪和油或有机材料中的一种或多种过氧化物质，活体样品中的脂肪过氧化物等可以直接在样品中或在用溶剂从活体样品中提取脂肪过氧化物之后，通过使过氧化物质与在由能够具有两个或多个化合价产生颜色的金属制成的金属化合物存在下的可氧化着色试剂，并以比色测定着色程度。

2. 发明授权

US4940660A Color developing method in clinical examinations 失效
标题翻译：彩色显影方法在临床检查
公开(公告)号：US4940660A
公开(公告)日：1990-07-10
申请号：US914834
申请日：1986-10-02
申请人： Hidematsu Hirai , Toshiro Hanada , Kazuhiko Yamanishi , Hideko Nohara
发明人： Hidematsu Hirai , Toshiro Hanada , Kazuhiko Yamanishi , Hideko Nohara
IPC分类号： C12Q1/28 , G01N33/548
CPC分类号： G01N33/548 , C12Q1/28
摘要： A process for the determination of peroxidase activity or hydrogen peroxide in a clinical sample colorometrically by adding to the sample a reduced form coenzyme, a reagent selected from amines, phenols, and naphthols, a compound which is colored in the reduced form, and peroxidase or an aqueous solution of hydrogen peroxide. The process is essentially unaffected by the presence of ascorbic acid, bilirubin or other reducing substances of body fluids.
摘要翻译：通过向样品中加入还原型辅酶，选自胺，酚和萘酚的试剂，还原形式着色的化合物，以及过氧化物酶或过氧化物酶或过氧化物酶过氧化氢水溶液。该方法基本上不受抗坏血酸，胆红素或其它体液还原物质的存在的影响。

3. 发明授权

US4910134A Ascorbic acid decomposing method 失效
标题翻译：抗坏血酸分解法
公开(公告)号：US4910134A
公开(公告)日：1990-03-20
申请号：US6223
申请日：1987-01-23
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： G01N33/82 , C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/28
摘要： A method for decomposing ascorbic acid in a sample is descirbed wherein in the method water and dehydroascorbic acid are produced without generation of hydrogen peroxide characterized in that the decomposition is conducted in the presence of copper ion, peroxidase and at least one other compound selected from the group consisting of:4-aminoantipyrine,3-methyl-2-benzothiazolinonehydrazone,2,2'-azinobis(3-ethylbenzothiazoline-6 sulphonic acid),a triphenyl methane type leuco compound,a phenol comound.an aniline compound, anda naphthol compound.
摘要翻译：描述了在样品中分解抗坏血酸的方法，其中在该方法中产生水和脱氢抗坏血酸，而不产生过氧化氢，其特征在于在铜离子，过氧化物酶和至少一种其它选自下列的化合物的存在下进行分解：由4-氨基安替比林，3-甲基-2-苯并噻唑酮腙，2,2'-氮杂双（3-乙基苯并噻唑啉-6-磺酸），三苯甲烷型无色化合物，苯酚化合物组成的组。苯胺化合物和萘酚化合物。

4. 发明授权

US4801538A Process for determining superoxide dismutase activity 失效
标题翻译：确定超氧化物歧化酶活性的方法
公开(公告)号：US4801538A
公开(公告)日：1989-01-31
申请号：US834971
申请日：1986-02-28
申请人： Toshiro Hanada , Kazuhiko Yamanishi
发明人： Toshiro Hanada , Kazuhiko Yamanishi
IPC分类号： C12Q1/26 , C12Q1/28 , C12N9/02 , C12N9/08
CPC分类号： C12Q1/28 , C12Q1/26
摘要： Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.
摘要翻译：通过使用超氧阴离子作为底物产生的过氧化氢和在电子载体存在下作用超氧化物歧化酶来测定超氧化物歧化酶活性。当马来酰亚胺或其衍生物，如果需要的话，羰基化合物，如果还需要溴离子和/或柠檬酸或其盐加入到电子载体中，校准曲线的灵敏度和线性进一步提高。

5. 发明授权

US4778753A Method of quantitatively measuring an oxidative substance using triphenyl methane type leuco-pigment as a coloring substance 失效
标题翻译：使用三苯甲烷型无色素作为着色物质定量测定氧化物质的方法
公开(公告)号：US4778753A
公开(公告)日：1988-10-18
申请号：US649479
申请日：1984-09-11
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： B41M5/136 , C09B11/12 , C12Q1/28 , C12Q1/26 , C12Q1/54 , C12Q1/60
CPC分类号： B41M5/1366 , C09B11/12 , C12Q1/28 , C12Q2326/40 , Y10S435/904 , Y10T436/206664
摘要： Disclosed herein is a method of quantitatively measuring an oxidative substance with avoiding influences of a coloring-interfering substance in the quantitative measurement of the oxidative substance by using a triphenyl methane type leuco coloring matter as a coloring reagent. Disclosed herein is also such an oxidative quantitative measurement in which the coloring sensitivity can be further adjusted. In order to avoid the influences of the coloring-interfering substance, there is employed at least one kind of (i) uricase, (ii) an anionic surface active agent and (iii) a metal chelate compound. As the triphenyl methane type leuco coloring matter, use may be made of a compound of the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent a hydrogen atom or a lower alkyl group, and X.sub.1 and X.sub.2, which may be the same as or different from each other, represent a hydrogen atom, --SO.sub.3 M.sub.1, --COOM.sub.2, --O(CH.sub.2).sub.m SO.sub.3 M.sub.3, --O(CH.sub.2).sub.n COOM.sub.4 or --N(R.sub.5) (R.sub.6) in which M.sub.1, M.sub.2, M.sub.3 and M.sub.4 represent a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, R.sub.5 and R.sub.6 independently represent a hydrogen atom or a lower alkyl group, and m and n represent an integer of 2-4. Azide compound is further added or the compound represented by the general formula (I) is included with cyclodextrin or modified dextrine to adjust coloring sensitivity.
摘要翻译：本发明公开了一种通过使用三苯甲烷型无色素作为着色试剂，可以避免着色干扰物质在氧化物质的定量测定中的影响而定量测定氧化物质的方法。本文公开了也可以进一步调节着色灵敏度的氧化定量测量。为了避免着色干扰物质的影响，使用至少一种（i）尿酸酶，（ii）阴离子表面活性剂和（iii）金属螯合物。作为三苯甲烷型无色素，可以使用通式（I）的化合物：其中R 1，R 2，R 3和R 4可以相同或不同。表示氢原子或低级烷基，X1和X2可以相同或不同，表示氢原子，-SO3M1，-COOM2，-O（CH2）mSO3M3，-O（CH2））其中M1，M2，M3和M4表示氢原子的nCOOM4或-N（R5）（R6），碱金属离子或NH4 +，R5和R6独立地表示氢原子或低级烷基，m和n 代表2-4的整数。进一步添加叠氮化合物，或者将通式（I）表示的化合物与环糊精或改性的糊精一起包括以调节着色敏感性。

6. 发明授权

US4837331A Stabilization of tetrazolium salts with cyclodextrins 失效
标题翻译：用环糊精稳定四唑鎓盐
公开(公告)号：US4837331A
公开(公告)日：1989-06-06
申请号：US930094
申请日：1986-11-13
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： G01N33/50 , C07D257/04 , C07D417/04 , C09B50/02 , C12Q1/26 , G01N31/22
CPC分类号： C07D257/04 , C09B50/02 , C12Q1/26
摘要： An aqueous solution containing a tetrazolium compound used for determining superoxide ions can be stabilized by adding .beta.-cyclodextrin and/or a derivative thereof and/or .gamma.-cyclodextrin.
摘要翻译：含有用于测定超氧化物离子的四唑鎓化合物的水溶液可以通过加入β-环糊精和/或其衍生物和/或γ-环糊精来稳定。

7. 发明授权

US4695539A Process for quantitative determination of substrate treated with oxidase 失效
标题翻译：用氧化酶处理的底物的定量测定方法
公开(公告)号：US4695539A
公开(公告)日：1987-09-22
申请号：US516241
申请日：1983-07-22
申请人： Yoshitsugu Sakata , Yoshibonu Miyashita , Tadashi Hamanaka , Hiroyuki Kodera , Yutaka Miki , Kazuhiko Yamanishi , Toshiro Hanada
发明人： Yoshitsugu Sakata , Yoshibonu Miyashita , Tadashi Hamanaka , Hiroyuki Kodera , Yutaka Miki , Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： G01N33/50 , C12Q1/26 , C12Q1/28 , C12Q1/54 , C12Q1/60 , C12Q1/62
CPC分类号： C12Q1/60 , C12Q1/26 , C12Q1/54 , C12Q1/62 , Y10S435/81
摘要： A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
摘要翻译：样品中的底物可以通过测量通过用相应于所要测定的底物的特定氧化酶处理样品而产生的比色超氧化物离子来测定，所述氧化酶处理和所测得的超氧化物离子是通过使用包含（ a）氧化酶，（b）过氧化物酶，（c）苯酚和/或胺，（d）硫醇化合物，（e）待还原的着色剂，以及必要时（f）螯合剂。

8. 发明授权

US4673635A Triphenyl methane derivatives and method of quantitatively measuring an oxidative substance 失效
标题翻译：三苯甲烷衍生物和定量测定氧化物质的方法
公开(公告)号：US4673635A
公开(公告)日：1987-06-16
申请号：US683487
申请日：1984-12-19
申请人： Kazuhiko Yamanishi , Akinori Shintani , Satoru Okajima , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Akinori Shintani , Satoru Okajima , Toshiro Hanada
IPC分类号： B41M5/136 , C09B11/00 , C09B11/12 , C09B11/14 , C12Q1/28 , C12Q1/26 , C12Q1/54 , C12Q1/60
CPC分类号： B41M5/1366 , C09B11/12 , C12Q1/28 , C12Q2326/40 , Y10S436/904 , Y10T436/206664
摘要： Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent lower alkyl groups, and X.sub.1 and X.sub.2 both represent --O(CH.sub.2).sub.n SO.sub.3 M in which M is a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, and n is an integer of 2-4, or either of X.sub.1 and X.sub.2 represents --O(CH.sub.2).sub.n SO.sub.3 M in which M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side. Disclosed is also a method of quantitatively measuring an oxidative substance, particularly, hydrogen peroxide, by using this color test reagent.
摘要翻译：本文公开了一种新颖的化合物，其在中性范围内对水的溶解性优异，在水溶液状态下长时间稳定，着色敏感性高，稳定的显色性，并且由通式（ I）：其中R 1，R 2，R 3和R 4可以相同或不同，表示低级烷基，X 1和X 2均表示-O（CH 2）n SO 3 M，其中M 是氢原子，碱金属离子或NH 4 +，n是2-4的整数，或者X 1和X 2中的任一个表示-O（CH 2）n SO 3 M，其中M和n与上述相同，而另一个表示氢原子。该试剂由于其在长波长侧具有吸收的颜色，几乎不受血清成分如血红蛋白，胆红素等的影响。还公开了通过使用该颜色试验剂定量测定氧化物质，特别是过氧化氢的方法。

9. 发明授权

US4879221A Process for determination of .gamma.-GTP activity 失效
标题翻译：测定γ-GTP活性的方法
公开(公告)号：US4879221A
公开(公告)日：1989-11-07
申请号：US902897
申请日：1986-09-02
申请人： Kuniaki Tokuda , Seiji Morii , Kazuhiko Yamanishi
发明人： Kuniaki Tokuda , Seiji Morii , Kazuhiko Yamanishi
IPC分类号： C08B37/16 , C12Q1/48
CPC分类号： C12Q1/48 , C08B37/0012 , Y10S435/81 , Y10T436/108331 , Y10T436/2525
摘要： .gamma.-GTP activity can be determined easily by using a stabilized substrate solution comprising .gamma.-L-glutamyl-p-nitroanilide and a modified cyclodextrin, together with an acceptor solution containing an acceptor for glutamic acid and measuring p-nitroaniline generated.

10. 发明授权

US4695540A Quantitative determination of substrate treated with oxidase 失效
标题翻译：用氧化酶处理的底物的定量测定
公开(公告)号：US4695540A
公开(公告)日：1987-09-22
申请号：US516271
申请日：1983-07-22
申请人： Kazuhiko Yamanishi , Toshiro Hanada
发明人： Kazuhiko Yamanishi , Toshiro Hanada
IPC分类号： C12Q1/26 , C12Q1/54 , C12Q1/60 , C12Q1/62 , C12Q1/28
CPC分类号： C12Q1/26 , C12Q1/54 , C12Q1/60 , C12Q1/62 , Y10S435/81
摘要： Cholesterol in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with cholesterol oxidase. Similarly, a substrate such as glucose, etc., can be determined quantitatively by measuring the generated superoxide ion colorimetrically, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b') iron complex of porphyrin or ion chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
摘要翻译：通过测量通过用胆固醇氧化酶处理样品产生的比色超氧化物离子，可以定量测定样品中的胆固醇。类似地，可以通过比色测定生成的超氧化物离子，通过使用包含（a）氧化酶（b'）的试剂组合物进行的所述氧化酶处理和测量所产生的超氧化物离子来定量测定诸如葡萄糖等的底物，）卟啉的络合物或络合物的离子螯合物，（c）胺和/或苯酚，（d）硫醇化合物，（e）待还原的着色剂，以及必要时（f）螯合剂。

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