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1. 发明申请

US20100086984A1 OPTOINJECTION METHODS 审中-公开
标题翻译：光学方法
公开(公告)号：US20100086984A1
公开(公告)日：2010-04-08
申请号：US12533801
申请日：2009-07-31
申请人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
发明人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
IPC分类号： C12N13/00
CPC分类号： C12N13/00 , C12M35/02 , C12M47/06 , C12N5/0087 , C12N5/0093 , C12N15/1034 , C12N15/87 , G01N33/5005 , G01N33/5091 , G01N33/56966
摘要： Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
摘要翻译：通过（a）照射框架中包含的细胞群来瞬时透化靶细胞的光注射方法; （b）检测从所述框架引导的光的至少一种性质; （c）通过光的属性定位靶细胞; 和（d）用辐射脉冲照射靶细胞。

2. 发明授权

US07622274B2 Method for determining a product secretion profile of cells 有权
标题翻译：确定细胞产物分泌谱的方法
公开(公告)号：US07622274B2
公开(公告)日：2009-11-24
申请号：US11842090
申请日：2007-08-20
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12Q1/24
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

3. 发明授权

US08218840B2 Method and device for selectively targeting cells within a three-dimensional specimen 有权
标题翻译：用于选择性地靶向三维样本内的细胞的方法和装置
公开(公告)号：US08218840B2
公开(公告)日：2012-07-10
申请号：US13030082
申请日：2011-02-17
申请人： Timothy M Eisfeld , Manfred R Koller , Bernhard O Palsson
发明人： Timothy M Eisfeld , Manfred R Koller , Bernhard O Palsson
IPC分类号： G06K9/00
CPC分类号： G06K9/00127 , A61N5/062 , A61N5/1069 , A61N2005/067 , G01N15/1468 , G01N15/1475 , G01N2015/1415 , G01N2015/144 , G01N2015/1443 , G01N2015/1445 , G01N2015/1452 , G01N2015/1472 , G01N2015/149
摘要： The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.
摘要翻译：本发明提供一种用于电磁影响样品中感兴趣的颗粒的装置。该装置包括（a）能够支撑样本的台架; （b）包括至少一个相机的检测器，其中所述检测器能够分辨所述样本内的感兴趣的粒子; （c）用于在三维中定位感兴趣的颗粒的装置; （d）用于将电磁辐射聚焦到样本内的焦点体积的装置; 和（e）用于调节舞台和电磁辐射聚焦装置的相对位置的装置，从而将感兴趣的颗粒定位在聚焦体积内。

4. 发明申请

US20110254948A1 METHOD AND DEVICE FOR SELECTIVELY TARGETING CELLS WITHIN A THREE -DIMENSIONAL SPECIMEN 有权
标题翻译：用于在三维样本中选择性细胞的方法和装置
公开(公告)号：US20110254948A1
公开(公告)日：2011-10-20
申请号：US13030082
申请日：2011-02-17
申请人： Timothy M. Eisfeld , Manfred R. Koller , Bernhard O. Palsson
发明人： Timothy M. Eisfeld , Manfred R. Koller , Bernhard O. Palsson
IPC分类号： G06K9/00 , H04N7/18
CPC分类号： G06K9/00127 , A61N5/062 , A61N5/1069 , A61N2005/067 , G01N15/1468 , G01N15/1475 , G01N2015/1415 , G01N2015/144 , G01N2015/1443 , G01N2015/1445 , G01N2015/1452 , G01N2015/1472 , G01N2015/149
摘要： The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.
摘要翻译：本发明提供一种用于电磁影响样品中感兴趣的颗粒的装置。该装置包括（a）能够支撑样本的台架; （b）包括至少一个相机的检测器，其中所述检测器能够分辨所述样本内的感兴趣的粒子; （c）用于在三维中定位感兴趣的颗粒的装置; （d）用于将电磁辐射聚焦到样本内的焦点体积的装置; 以及（e）用于调整舞台和电磁辐射聚焦装置的相对位置的装置，从而将感兴趣的颗粒定位在聚焦体积内。

5. 发明申请

US20100136683A1 METHODS FOR ISOLATING CELLS BASED ON PRODUCT SECRETION 有权
标题翻译：基于产品分离的细胞分离方法
公开(公告)号：US20100136683A1
公开(公告)日：2010-06-03
申请号：US12603413
申请日：2009-10-21
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12N5/078
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

6. 发明授权

US07505618B2 Method and apparatus for selectively targeting specific cells within a cell population 有权
标题翻译：用于选择性靶向细胞群体内特定细胞的方法和装置
公开(公告)号：US07505618B2
公开(公告)日：2009-03-17
申请号：US10359483
申请日：2003-02-04
申请人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld
发明人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld
IPC分类号： G06K9/00
CPC分类号： C12N13/00 , C12M35/02 , C12M41/46 , C12N5/0087 , C12N5/0093 , C12N15/1034 , G01N15/1434 , G01N21/6428 , G01N21/645 , G01N21/6456 , G01N33/5005 , G01N33/5091 , G01N33/56966 , G01N2021/1725 , Y10S128/922
摘要： This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.
摘要翻译：本发明提供了一种用于选择性地识别和靶向细胞群体内的特定细胞的能量束的方法和装置，以便在目标细胞中诱导反应。使用本发明，可以鉴定和影响群体中的每个可检测细胞，而基本上不影响混合物内的非靶细胞。

7. 发明授权

US06514722B2 Method and apparatus for selectively targeting specific cells within a cell population 有权
公开(公告)号：US06514722B2
公开(公告)日：2003-02-04
申请号：US09728281
申请日：2000-11-30
申请人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld
发明人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld
IPC分类号： G01N33487
CPC分类号： C12N13/00 , C12M35/02 , C12M41/46 , C12N5/0087 , C12N5/0093 , C12N15/1034 , G01N15/1434 , G01N21/6428 , G01N21/645 , G01N21/6456 , G01N33/5005 , G01N33/5091 , G01N33/56966 , G01N2021/1725 , Y10S128/922
摘要： This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.

8. 发明授权

US5616487A Stabilized retrovirus compositions 失效
标题翻译：稳定的逆转录病毒组合物
公开(公告)号：US5616487A
公开(公告)日：1997-04-01
申请号：US307862
申请日：1994-09-15
申请人： Bernhard O. Palsson , Timothy M. Eisfeld
发明人： Bernhard O. Palsson , Timothy M. Eisfeld
IPC分类号： A61K48/00 , C12N7/00 , C12N7/01 , C12N15/867 , C12N5/10
CPC分类号： C12N15/86 , C12N7/00 , A61K48/00 , C12N2740/13021 , C12N2740/13043 , C12N2740/13051
摘要： The present invention provides a stabilized virus, which is modified using a stabilizing agent. The invention also provides a process for producing a stabilized virus by culturing viral producing cells with a stabilizing agent at a temperature below 37.degree. C. The invention further provides methods to introduce an exogenous nucleotide sequence into a cell using a stabilized virus containing the exogenous nucleotide sequence. The invention also provides methods for administering an exogenous nucleotide sequence to a subject using a stabilized virus containing the exogenous nucleotide sequence. The invention further provides a method to produce a protein by infecting a cell with a stabilized virus containing a exogenous nucleotide sequence encoding the protein and then isolating the protein produced by the infected cells.
摘要翻译：本发明提供了使用稳定剂改性的稳定的病毒。本发明还提供了通过在37℃以下的温度下用稳定剂培养病毒产生细胞来生产稳定的病毒的方法。本发明还提供了使用含有外源核苷酸的稳定化病毒将外源核苷酸序列引入细胞的方法序列。本发明还提供了使用含有外源核苷酸序列的稳定化病毒向受试者施用外源核苷酸序列的方法。本发明还提供了通过用含有编码该蛋白质的外源核苷酸序列的稳定化病毒感染细胞，然后分离由感染细胞产生的蛋白质来生产蛋白质的方法。

9. 发明申请

US20090175529A1 METHOD AND APPARATUS FOR SELECTIVELY TARGETING SPECIFIC CELLS WITHIN A CELL POPULATION 审中-公开
标题翻译：在细胞群体中选择性特异性细胞的方法和装置
公开(公告)号：US20090175529A1
公开(公告)日：2009-07-09
申请号：US12405875
申请日：2009-03-17
申请人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld , Robert A. O'Neal
发明人： Bernhard O. Palsson , Manfred R. Koller , Timothy M. Eisfeld , Robert A. O'Neal
IPC分类号： G06K9/00
CPC分类号： C12N13/00 , C12M35/02 , C12M41/46 , C12N5/0087 , C12N5/0093 , C12N15/1034 , G01N15/1434 , G01N21/6428 , G01N21/645 , G01N21/6456 , G01N33/5005 , G01N33/5091 , G01N33/56966 , G01N2021/1725 , Y10S128/922
摘要： This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.
摘要翻译：本发明提供了一种用于选择性地识别和靶向细胞群体内的特定细胞的能量束的方法和装置，以便在目标细胞中诱导反应。使用本发明，可以鉴定和影响群体中的每个可检测细胞，而基本上不影响混合物内的非靶细胞。

10. 发明授权

US07425426B2 Methods for purification of cells based on product secretion 有权
标题翻译：基于产品分泌来净化细胞的方法
公开(公告)号：US07425426B2
公开(公告)日：2008-09-16
申请号：US10801931
申请日：2004-03-15
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12Q1/24
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

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