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1. 发明申请

US20110201075A1 OPTOINJECTION METHODS 审中-公开
标题翻译：光学方法
公开(公告)号：US20110201075A1
公开(公告)日：2011-08-18
申请号：US12972305
申请日：2010-12-17
申请人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
发明人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
IPC分类号： C12N13/00
CPC分类号： C12N13/00 , C12M35/02 , C12M47/06 , C12N5/0087 , C12N5/0093 , C12N15/1034 , C12N15/87 , G01N33/5005 , G01N33/5091 , G01N33/56966
摘要： Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
摘要翻译：通过（a）照射框架中包含的细胞群来瞬时透化靶细胞的光注射方法; （b）检测从所述框架引导的光的至少一种性质; （c）通过光的属性定位靶细胞; 和（d）用辐射脉冲照射靶细胞。

2. 发明申请

US20100136683A1 METHODS FOR ISOLATING CELLS BASED ON PRODUCT SECRETION 有权
标题翻译：基于产品分离的细胞分离方法
公开(公告)号：US20100136683A1
公开(公告)日：2010-06-03
申请号：US12603413
申请日：2009-10-21
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12N5/078
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

3. 发明申请

US20100086984A1 OPTOINJECTION METHODS 审中-公开
标题翻译：光学方法
公开(公告)号：US20100086984A1
公开(公告)日：2010-04-08
申请号：US12533801
申请日：2009-07-31
申请人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
发明人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
IPC分类号： C12N13/00
CPC分类号： C12N13/00 , C12M35/02 , C12M47/06 , C12N5/0087 , C12N5/0093 , C12N15/1034 , C12N15/87 , G01N33/5005 , G01N33/5091 , G01N33/56966
摘要： Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
摘要翻译：通过（a）照射框架中包含的细胞群来瞬时透化靶细胞的光注射方法; （b）检测从所述框架引导的光的至少一种性质; （c）通过光的属性定位靶细胞; 和（d）用辐射脉冲照射靶细胞。

4. 发明授权

US07622274B2 Method for determining a product secretion profile of cells 有权
标题翻译：确定细胞产物分泌谱的方法
公开(公告)号：US07622274B2
公开(公告)日：2009-11-24
申请号：US11842090
申请日：2007-08-20
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12Q1/24
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

5. 发明授权

US07425426B2 Methods for purification of cells based on product secretion 有权
标题翻译：基于产品分泌来净化细胞的方法
公开(公告)号：US07425426B2
公开(公告)日：2008-09-16
申请号：US10801931
申请日：2004-03-15
申请人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Annabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12Q1/24
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

6. 发明授权

US07300795B2 Optoinjection methods 有权
标题翻译：注射方法
公开(公告)号：US07300795B2
公开(公告)日：2007-11-27
申请号：US10814966
申请日：2004-03-30
申请人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
发明人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard Ø. Palsson
IPC分类号： C12N15/87
CPC分类号： C12N13/00 , C12M35/02 , C12M47/06 , C12N5/0087 , C12N5/0093 , C12N15/1034 , C12N15/87 , G01N33/5005 , G01N33/5091 , G01N33/56966
摘要： Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
摘要翻译：通过（a）照射框架中包含的细胞群来瞬时透化靶细胞的光注射方法; （b）检测从所述框架引导的光的至少一种性质; （c）通过光的属性定位靶细胞; 和（d）用辐射脉冲照射靶细胞。

7. 发明授权

US08236521B2 Methods for isolating cells based on product secretion 有权
标题翻译：基于产品分泌分离细胞的方法
公开(公告)号：US08236521B2
公开(公告)日：2012-08-07
申请号：US12603413
申请日：2009-10-21
申请人： Manfred R. Koller , Elie G. Hanania , Anabeth Fieck , Timothy M. Eisfeld
发明人： Manfred R. Koller , Elie G. Hanania , Anabeth Fieck , Timothy M. Eisfeld
IPC分类号： C12Q1/24
CPC分类号： C12N13/00
摘要： The invention provides methods for purifying one or more cells based on the level of one or more products secreted by the cells. In one embodiment, the method involves (a) contacting a plurality of cells immobilized in proximity to a capture matrix, the capture matrix capable of localizing a product secreted by one or more of the cells, with an agent that selectively binds to the product, the agent capable of generating a signal detectable as a property of light; (b) illuminating a population of the cells, the population contained in a frame; (c) detecting two or more properties of light directed from the frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light, and (e) irradiating the non-selected cells, wherein each non-selected cell receives a substantially lethal dose of radiation, whereby one or more selected cells having a desired product secretion profile are purified.
摘要翻译：本发明提供了基于由细胞分泌的一种或多种产物的水平纯化一种或多种细胞的方法。在一个实施方案中，该方法包括（a）使固定在捕获基质附近的多个细胞与能够选择性结合产物的试剂接触，所述捕获基质能够将由一种或多种细胞分泌的产物定位，所述试剂能够产生可检测为光的性质的信号; （b）照亮一群人群，包含在一个框架内的群体; （c）检测从所述框架引导的光的两个或多个属性，其中光的第一特性基本上标识所述群体的所有细胞，并且所述光的第二属性识别定位于所述捕获矩阵的产品; （d）参照检测到的第一性质，将（i）基本上所有细胞的群体定位，以及（ii）参照检测到的第二属性的一个或多个所选择的细胞，和（e）选择的细胞，其中每个未选择的细胞接受基本致死的辐射剂量，从而纯化具有所需产物分泌曲线的一个或多个选择的细胞。

8. 发明授权

US06753161B2 Optoinjection methods 有权
标题翻译：注射方法
公开(公告)号：US06753161B2
公开(公告)日：2004-06-22
申请号：US09961691
申请日：2001-09-21
申请人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard O. Palsson
发明人： Manfred R. Koller , Elie G. Hanania , Timothy M. Eisfeld , Bernhard O. Palsson
IPC分类号： G01N3348
CPC分类号： C12N13/00 , C12M35/02 , C12M47/06 , C12N5/0087 , C12N5/0093 , C12N15/1034 , C12N15/87 , G01N33/5005 , G01N33/5091 , G01N33/56966
摘要： Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
摘要翻译：通过（a）照射框架中包含的细胞群来瞬时透化靶细胞的光注射方法; （b）检测从所述框架引导的光的至少一种性质; （c）通过光的属性定位靶细胞; 和（d）用辐射脉冲照射靶细胞。

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