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1. 发明申请

WO2011056060A8 RECOMBINANT MATRIX PROTEIN OF NIPAH VIRUS 审中-公开
标题翻译： NIPAH病毒的重组基质蛋白
公开(公告)号：WO2011056060A8
公开(公告)日：2012-06-14
申请号：PCT/MY2010000211
申请日：2010-10-19
申请人： UNIV PUTRA MALAYSIA , TAN WEN SIANG , SUBRAMANIAM SENTHIL KUMAR , TEY BENG TI , HAMID MUHAJIR
发明人： TAN WEN SIANG , SUBRAMANIAM SENTHIL KUMAR , TEY BENG TI , HAMID MUHAJIR
IPC分类号： C07K14/115 , A61K39/155 , C12N15/45 , C12N15/63 , C12Q1/70
CPC分类号： C07K16/1027 , A61K39/00 , C07K14/005 , C12N2760/18222
摘要： The present invention provides a recombinant matrix protein of Nipah virus containing matrix protein of Nipah virus which includes at least one His tag and myc epitope. Further, the present invention also provides a recombinant plasmid including recombinant matrix protein of Nipah virus. Also provided is a method for producing recombinant matrix protein of Nipah virus which includes the steps of (a) culturing expression host containing expression vector of recombinant matrix protein in a medium which allows growth of the expression host, (b) allowing the recombinant matrix protein to be expressed in the expression host, (c) harvesting the expression host from step (b) from the medium, (d) disrupting the expression host harvested from step (c) in a medium for sonication purposes and (e) purifying the recombinant matrix protein obtained from step (d). Also provided is a method for purifying recombinant matrix protein of Nipah virus which includes the steps of (a) harvesting and lysing recombinant expression host containing recombinant matrix protein cells, (b) purifying the recombinant matrix protein using nickel affinity column chromatography, (c) dialyzing the purified recombinant matrix protein from step (b) with buffer solution, (d) fractionating the purified recombinant matrix protein from step (c) using centrifugation and (e) collecting and pooling the fractionated recombinant matrix protein obtained from step (d) concentration of protein. There is also provided a pharmaceutical composition comprising a therapeutically effective amount of recombinant matrix protein of Nipah virus. Also provided is a diagnostic reagent comprising an effective amount of recombinant matrix protein matrix protein of Nipah virus.
摘要翻译：本发明提供了含有尼帕病毒基质蛋白的尼帕病毒的重组基质蛋白，其包含至少一个His标签和myc表位。此外，本发明还提供包含Nipah病毒的重组基质蛋白质的重组质粒。还提供了一种制备尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）在允许表达宿主生长的培养基中培养含有重组基质蛋白表达载体的表达宿主，（b）使重组基质蛋白在表达宿主中表达，（c）从培养基中收获来自步骤（b）的表达宿主，（d）在用于超声处理的培养基中破坏从步骤（c）收获的表达宿主，和（e）纯化重组体（d）获得的基质蛋白。还提供了一种用于纯化尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）收获和裂解含有重组基质蛋白细胞的重组表达宿主，（b）使用镍亲和柱色谱纯化重组基质蛋白，（c）用步骤（b）用缓冲溶液透析纯化的重组基质蛋白，（d）使用离心法从步骤（c）中分离纯化的重组基质蛋白，（e）收集和合并步骤（d）浓度得到的分级重组基质蛋白的蛋白质。还提供了包含治疗有效量的尼帕病毒重组基质蛋白的药物组合物。还提供了包含有效量的Nipah病毒的重组基质蛋白质基质蛋白质的诊断试剂。

2. 发明申请

WO2011056060A3 RECOMBINANT MATRIX PROTEIN OF NIPAH VIRUS 审中-公开
标题翻译： NIPAH病毒的重组基质蛋白
公开(公告)号：WO2011056060A3
公开(公告)日：2011-12-15
申请号：PCT/MY2010000211
申请日：2010-10-19
申请人： UNIV PUTRA MALAYSIA , TAN WEN SIANG , SUBRAMANIAM SENTHIL KUMAR , TEY BENG TI , HAMID MUHAJIR
发明人： TAN WEN SIANG , SUBRAMANIAM SENTHIL KUMAR , TEY BENG TI , HAMID MUHAJIR
IPC分类号： C07K14/115 , A61K39/155 , C12N15/45 , C12N15/63 , C12Q1/70
CPC分类号： C07K16/1027 , A61K39/00 , C07K14/005 , C12N2760/18222
摘要： The present invention provides a recombinant matrix protein of Nipah virus containing matrix protein of Nipah virus which includes at least one His tag and myc epitope. Further, the present invention also provides a recombinant plasmid including recombinant matrix protein of Nipah virus. Also provided is a method for producing recombinant matrix protein of Nipah virus which includes the steps of (a) culturing expression host containing expression vector of recombinant matrix protein in a medium which allows growth of the expression host, (b) allowing the recombinant matrix protein to be expressed in the expression host, (c) harvesting the expression host from step (b) from the medium, (d) disrupting the expression host harvested from step (c) in a medium for sonication purposes and (e) purifying the recombinant matrix protein obtained from step (d). Also provided is a method for purifying recombinant matrix protein of Nipah virus which includes the steps of (a) harvesting and lysing recombinant expression host containing recombinant matrix protein cells, (b) purifying the recombinant matrix protein using nickel affinity column chromatography, (c) dialyzing the purified recombinant matrix protein from step (b) with buffer solution, (d) fractionating the purified recombinant matrix protein from step (c) using centrifugation and (e) collecting and pooling the fractionated recombinant matrix protein obtained from step (d) concentration of protein. There is also provided a pharmaceutical composition comprising a therapeutically effective amount of recombinant matrix protein of Nipah virus. Also provided is a diagnostic reagent comprising an effective amount of recombinant matrix protein matrix protein of Nipah virus.
摘要翻译：本发明提供了含有至少一个His标签和myc表位的Nipah病毒基质蛋白的Nipah病毒重组基质蛋白。此外，本发明还提供了包含Nipah病毒的重组基质蛋白的重组质粒。还提供了生产Nipah病毒重组基质蛋白的方法，其包括以下步骤：（a）在允许表达宿主生长的培养基中培养包含重组基质蛋白表达载体的表达宿主，（b）使重组基质蛋白（c）从培养基中收获来自步骤（b）的表达宿主，（d）在培养基中破碎步骤（c）中收获的表达宿主以达到超声处理目的，和（e）纯化重组体从步骤（d）获得的基质蛋白。本发明还提供了纯化Nipah病毒重组基质蛋白的方法，其包括以下步骤：（a）收获并裂解含重组基质蛋白细胞的重组表达宿主，（b）使用镍亲和柱层析纯化重组基质蛋白，（c）（d）使用离心分离来自步骤（c）的纯化的重组基质蛋白，和（e）收集并合并步骤（d）获得的分级重组基质蛋白的蛋白质。还提供了包含治疗有效量的尼帕病毒重组基质蛋白的药物组合物。还提供了包含有效量的Nipah病毒的重组基质蛋白基质蛋白的诊断试剂。

3. 发明申请

WO2011014051A1 A METHOD FOR CONTROLLING PROTEOLYTIC DEGRADATION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：一种控制重组蛋白质蛋白质降解的方法
公开(公告)号：WO2011014051A1
公开(公告)日：2011-02-03
申请号：PCT/MY2009/000105
申请日：2009-07-30
申请人： UNIVERSITI PUTRA MALAYSIA , TEY, Beng, Ti , CHONG, Fui, Chin , TAN, Wen, Siang
发明人： TEY, Beng, Ti , CHONG, Fui, Chin , TAN, Wen, Siang
IPC分类号： G01N33/68
CPC分类号： G01N33/6818 , G01N2333/115
摘要： The present invention provides a method to enhance the recovery of recombinant N protein of Nipah virus produced in Escherichia coli , in which by identifying and inhibiting the specific proteases in the cell lysate are achieved by an integrated use of a bioinformatics tool and a protease inhibition experimental setup.
摘要翻译：本发明提供了一种增强在大肠杆菌中产生的尼帕病毒重组N蛋白的回收的方法，其中通过鉴定和抑制细胞裂解物中的特异性蛋白酶是通过综合使用生物信息学工具和蛋白酶抑制实验来实现的建立。

4. 发明申请

WO2011081513A3 LIPASE PRODUCTION AND PURIFICATION 审中-公开
标题翻译：脂肪酶生产和净化
公开(公告)号：WO2011081513A3
公开(公告)日：2011-09-09
申请号：PCT/MY2010000256
申请日：2010-11-09
申请人： UNIV PUTRA MALAYSIA , LING TAU CHUAN , OOI CHIEN WEI , TEY BENG TI , ARIFF ARBAKARIYA , HII SIEW LING
发明人： LING TAU CHUAN , OOI CHIEN WEI , TEY BENG TI , ARIFF ARBAKARIYA , HII SIEW LING
IPC分类号： C12N9/20 , C12N1/20 , C12P21/00
CPC分类号： C12N9/20 , C12N1/20
摘要： The invention provides a process for extractive fermentation using integrated aqueous two-phase system for the simultaneous upstream of microbial fermentation and downstream processing of extracellular lipase derived from Burkholderia sp. The medium used for this extractive fermentation comprises components of fermentation media and a composition for aqueous two-phase system (ATPS). The two-phase fermentation system was used to extract extracellular lipase, which is produced continuously from the bacteria. The extractive fermentation includes the steps of (a) preparing an immiscible medium, wherein the medium includes fermentation media and ATPS forming reagents; (b) inoculating Burkholderia sp. into the medium as prepared in step (a); (c) agitating the medium prepared from step (b); (d) separating the medium from step (c) into two-phases for extracellular lipase purification; and (e) recovering the extracellular lipase from the medium.
摘要翻译：本发明提供了一种使用整合的含水两相系统的萃取发酵方法，用于微生物发酵的同时上游和源自伯克霍尔德氏菌属的细胞外脂肪酶的下游加工。用于这种提取发酵的培养基包含发酵培养基的组分和用于水性两相系统（ATPS）的组合物。使用两相发酵系统提取细菌连续产生的细胞外脂肪酶。提取发酵包括以下步骤：（a）制备不混溶的培养基，其中培养基包含发酵培养基和ATPS形成试剂; （b）接种Burkholderia sp。（a）中制备的介质中; （c）搅拌由步骤（b）制备的介质; （d）将来自步骤（c）的培养基分离成胞外脂酶纯化的两相; 和（e）从培养基中回收细胞外脂肪酶。

5. 发明申请

WO2011081513A2 LIPASE PRODUCTION AND PURIFICATION 审中-公开
标题翻译：油脂生产和纯化
公开(公告)号：WO2011081513A2
公开(公告)日：2011-07-07
申请号：PCT/MY2010/000256
申请日：2010-11-09
申请人： UNIVERSITI PUTRA MALAYSIA , LING, Tau Chuan , OOI , Chien Wei , TEY, Beng Ti , ARIFF , Arbakariya , HII, Siew Ling
发明人： LING, Tau Chuan , OOI , Chien Wei , TEY, Beng Ti , ARIFF , Arbakariya , HII, Siew Ling
IPC分类号： C12N9/20 , C12P21/00 , C12N1/20
CPC分类号： C12N9/20 , C12N1/20
摘要： The invention provides a process for extractive fermentation using integrated aqueous two-phase system for the simultaneous upstream of microbial fermentation and downstream processing of extracellular lipase derived from Burkholderia sp . The medium used for this extractive fermentation comprises components of fermentation media and a composition for aqueous two-phase system (ATPS). The two-phase fermentation system was used to extract extracellular lipase, which is produced continuously from the bacteria. The extractive fermentation includes the steps of (a) preparing an immiscible medium, wherein the medium includes fermentation media and ATPS forming reagents; (b) inoculating Burkholderia sp . into the medium as prepared in step (a); (c) agitating the medium prepared from step (b); (d) separating the medium from step (c) into two-phases for extracellular lipase purification; and (e) recovering the extracellular lipase from the medium.
摘要翻译：本发明提供了一种采用综合水相两相系统进行萃取发酵的方法，用于同时上游的微生物发酵和下游加工得自伯克霍尔德氏菌属的细胞外脂肪酶。用于该提取发酵的培养基包括发酵培养基的组分和用于水相两相体系（ATPS）的组合物。使用两相发酵系统提取从细菌连续生产的细胞外脂肪酶。提取发酵包括以下步骤：（a）制备不混溶培养基，其中培养基包括发酵培养基和ATPS形成试剂; （b）接种伯克霍尔德氏菌属进入步骤（a）中制备的培养基中; （c）搅拌由步骤（b）制备的培养基; （d）将步骤（c）中的培养基分离成两相用于细胞外脂肪酶纯化; 和（e）从培养基中回收细胞外脂肪酶。

6. 发明申请

WO2011056060A2 RECOMBINANT MATRIX PROTEIN OF NIPAH VIRUS 审中-公开
标题翻译： NIPAH病毒的重组基质蛋白
公开(公告)号：WO2011056060A2
公开(公告)日：2011-05-12
申请号：PCT/MY2010/000211
申请日：2010-10-19
申请人： UNIVERSITI PUTRA MALAYSIA , TAN, Wen Siang , SUBRAMANIAM, Senthil Kumar , TEY, Beng Ti , HAMID, Muhajir
发明人： TAN, Wen Siang , SUBRAMANIAM, Senthil Kumar , TEY, Beng Ti , HAMID, Muhajir
IPC分类号： C07K14/115 , C12N15/45 , C12N15/63 , A61K39/155 , C12Q1/70
CPC分类号： C07K16/1027 , A61K39/00 , C07K14/005 , C12N2760/18222
摘要： The present invention provides a recombinant matrix protein of Nipah virus containing matrix protein of Nipah virus which includes at least one His tag and myc epitope. Further, the present invention also provides a recombinant plasmid including recombinant matrix protein of Nipah virus. Also provided is a method for producing recombinant matrix protein of Nipah virus which includes the steps of (a) culturing expression host containing expression vector of recombinant matrix protein in a medium which allows growth of the expression host, (b) allowing the recombinant matrix protein to be expressed in the expression host, (c) harvesting the expression host from step (b) from the medium, (d) disrupting the expression host harvested from step (c) in a medium for sonication purposes and (e) purifying the recombinant matrix protein obtained from step (d). Also provided is a method for purifying recombinant matrix protein of Nipah virus which includes the steps of (a) harvesting and lysing recombinant expression host containing recombinant matrix protein cells, (b) purifying the recombinant matrix protein using nickel affinity column chromatography, (c) dialyzing the purified recombinant matrix protein from step (b) with buffer solution, (d) fractionating the purified recombinant matrix protein from step (c) using centrifugation and (e) collecting and pooling the fractionated recombinant matrix protein obtained from step (d) concentration of protein. There is also provided a pharmaceutical composition comprising a therapeutically effective amount of recombinant matrix protein of Nipah virus. Also provided is a diagnostic reagent comprising an effective amount of recombinant matrix protein matrix protein of Nipah virus.
摘要翻译：本发明提供了含有尼帕病毒基质蛋白的尼帕病毒的重组基质蛋白，其包含至少一个His标签和myc表位。此外，本发明还提供包含Nipah病毒的重组基质蛋白质的重组质粒。还提供了一种制备尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）在允许表达宿主生长的培养基中培养含有重组基质蛋白表达载体的表达宿主，（b）使重组基质蛋白在表达宿主中表达，（c）从培养基中收获来自步骤（b）的表达宿主，（d）在用于超声处理的培养基中破坏从步骤（c）收获的表达宿主，和（e）纯化重组体（d）获得的基质蛋白。还提供了一种用于纯化尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）收获和裂解含有重组基质蛋白细胞的重组表达宿主，（b）使用镍亲和柱色谱纯化重组基质蛋白，（c）用步骤（b）用缓冲溶液透析纯化的重组基质蛋白，（d）使用离心法从步骤（c）中分离纯化的重组基质蛋白，（e）收集和合并步骤（d）浓度得到的分级重组基质蛋白的蛋白质。还提供了包含治疗有效量的尼帕病毒重组基质蛋白的药物组合物。还提供了包含有效量的Nipah病毒的重组基质蛋白质基质蛋白质的诊断试剂。

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