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1. 发明申请

WO2011056060A2 RECOMBINANT MATRIX PROTEIN OF NIPAH VIRUS 审中-公开
标题翻译： NIPAH病毒的重组基质蛋白
公开(公告)号：WO2011056060A2
公开(公告)日：2011-05-12
申请号：PCT/MY2010/000211
申请日：2010-10-19
申请人： UNIVERSITI PUTRA MALAYSIA , TAN, Wen Siang , SUBRAMANIAM, Senthil Kumar , TEY, Beng Ti , HAMID, Muhajir
发明人： TAN, Wen Siang , SUBRAMANIAM, Senthil Kumar , TEY, Beng Ti , HAMID, Muhajir
IPC分类号： C07K14/115 , C12N15/45 , C12N15/63 , A61K39/155 , C12Q1/70
CPC分类号： C07K16/1027 , A61K39/00 , C07K14/005 , C12N2760/18222
摘要： The present invention provides a recombinant matrix protein of Nipah virus containing matrix protein of Nipah virus which includes at least one His tag and myc epitope. Further, the present invention also provides a recombinant plasmid including recombinant matrix protein of Nipah virus. Also provided is a method for producing recombinant matrix protein of Nipah virus which includes the steps of (a) culturing expression host containing expression vector of recombinant matrix protein in a medium which allows growth of the expression host, (b) allowing the recombinant matrix protein to be expressed in the expression host, (c) harvesting the expression host from step (b) from the medium, (d) disrupting the expression host harvested from step (c) in a medium for sonication purposes and (e) purifying the recombinant matrix protein obtained from step (d). Also provided is a method for purifying recombinant matrix protein of Nipah virus which includes the steps of (a) harvesting and lysing recombinant expression host containing recombinant matrix protein cells, (b) purifying the recombinant matrix protein using nickel affinity column chromatography, (c) dialyzing the purified recombinant matrix protein from step (b) with buffer solution, (d) fractionating the purified recombinant matrix protein from step (c) using centrifugation and (e) collecting and pooling the fractionated recombinant matrix protein obtained from step (d) concentration of protein. There is also provided a pharmaceutical composition comprising a therapeutically effective amount of recombinant matrix protein of Nipah virus. Also provided is a diagnostic reagent comprising an effective amount of recombinant matrix protein matrix protein of Nipah virus.
摘要翻译：本发明提供了含有尼帕病毒基质蛋白的尼帕病毒的重组基质蛋白，其包含至少一个His标签和myc表位。此外，本发明还提供包含Nipah病毒的重组基质蛋白质的重组质粒。还提供了一种制备尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）在允许表达宿主生长的培养基中培养含有重组基质蛋白表达载体的表达宿主，（b）使重组基质蛋白在表达宿主中表达，（c）从培养基中收获来自步骤（b）的表达宿主，（d）在用于超声处理的培养基中破坏从步骤（c）收获的表达宿主，和（e）纯化重组体（d）获得的基质蛋白。还提供了一种用于纯化尼帕病毒重组基质蛋白的方法，其包括以下步骤：（a）收获和裂解含有重组基质蛋白细胞的重组表达宿主，（b）使用镍亲和柱色谱纯化重组基质蛋白，（c）用步骤（b）用缓冲溶液透析纯化的重组基质蛋白，（d）使用离心法从步骤（c）中分离纯化的重组基质蛋白，（e）收集和合并步骤（d）浓度得到的分级重组基质蛋白的蛋白质。还提供了包含治疗有效量的尼帕病毒重组基质蛋白的药物组合物。还提供了包含有效量的Nipah病毒的重组基质蛋白质基质蛋白质的诊断试剂。

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