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1. 发明申请

WO2011081513A2 LIPASE PRODUCTION AND PURIFICATION 审中-公开
标题翻译：油脂生产和纯化
公开(公告)号：WO2011081513A2
公开(公告)日：2011-07-07
申请号：PCT/MY2010/000256
申请日：2010-11-09
申请人： UNIVERSITI PUTRA MALAYSIA , LING, Tau Chuan , OOI , Chien Wei , TEY, Beng Ti , ARIFF , Arbakariya , HII, Siew Ling
发明人： LING, Tau Chuan , OOI , Chien Wei , TEY, Beng Ti , ARIFF , Arbakariya , HII, Siew Ling
IPC分类号： C12N9/20 , C12P21/00 , C12N1/20
CPC分类号： C12N9/20 , C12N1/20
摘要： The invention provides a process for extractive fermentation using integrated aqueous two-phase system for the simultaneous upstream of microbial fermentation and downstream processing of extracellular lipase derived from Burkholderia sp . The medium used for this extractive fermentation comprises components of fermentation media and a composition for aqueous two-phase system (ATPS). The two-phase fermentation system was used to extract extracellular lipase, which is produced continuously from the bacteria. The extractive fermentation includes the steps of (a) preparing an immiscible medium, wherein the medium includes fermentation media and ATPS forming reagents; (b) inoculating Burkholderia sp . into the medium as prepared in step (a); (c) agitating the medium prepared from step (b); (d) separating the medium from step (c) into two-phases for extracellular lipase purification; and (e) recovering the extracellular lipase from the medium.
摘要翻译：本发明提供了一种采用综合水相两相系统进行萃取发酵的方法，用于同时上游的微生物发酵和下游加工得自伯克霍尔德氏菌属的细胞外脂肪酶。用于该提取发酵的培养基包括发酵培养基的组分和用于水相两相体系（ATPS）的组合物。使用两相发酵系统提取从细菌连续生产的细胞外脂肪酶。提取发酵包括以下步骤：（a）制备不混溶培养基，其中培养基包括发酵培养基和ATPS形成试剂; （b）接种伯克霍尔德氏菌属进入步骤（a）中制备的培养基中; （c）搅拌由步骤（b）制备的培养基; （d）将步骤（c）中的培养基分离成两相用于细胞外脂肪酶纯化; 和（e）从培养基中回收细胞外脂肪酶。

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