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1. 发明公开

EP2751277A2 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
标题翻译： MIKROFLUIDSYSTEM ZUR ENZYMDETEKTION
公开(公告)号：EP2751277A2
公开(公告)日：2014-07-09
申请号：EP12758395.3
申请日：2012-08-31
申请人： Koch, Jørn, Erland , Stougaard, Magnus , Andersen, Birgitta Ruth , Juul, Sissel , Leong, Kam , Ho, Megan Yi-ping
发明人： ANDERSEN, Felicie F. , KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6893 , C12Q1/00 , C12Q1/025 , C12Q1/04 , C12Q1/689 , G01N33/573 , Y02A50/58
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.
摘要翻译：提供微流体实施的检测酶特别是DNA修饰酶的方法，以及检测细胞或表达所述酶的微生物的方法。通过提供由该酶特异性靶向的核酸底物来检测该酶。

2. 发明公开

EP2702174A2 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 有权
标题翻译：系统，用于微生物鉴定和检测传染病
公开(公告)号：EP2702174A2
公开(公告)日：2014-03-05
申请号：EP12725607.1
申请日：2012-04-27
申请人： Koch, Jørn, Erland , Stougaard, Magnus , Knudsen, Birgitta, R.
发明人： ANDERSEN, Felicie, F. , KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R.
IPC分类号： C12Q1/68
CPC分类号： G01N33/56905 , C12Q1/533 , C12Q1/689 , C12Q1/6893 , C12Q2600/136 , G01N33/5695 , G01N2333/35 , G01N2333/445 , Y02A50/58
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.

3. 发明申请

WO2013029631A3 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
公开(公告)号：WO2013029631A3
公开(公告)日：2013-03-07
申请号：PCT/DK2012/050327
申请日：2012-08-31
申请人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
发明人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
IPC分类号： C12Q1/00
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

4. 发明申请

WO2012149936A3 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 审中-公开
公开(公告)号：WO2012149936A3
公开(公告)日：2012-11-08
申请号：PCT/DK2012/000056
申请日：2012-04-27
申请人： KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R. , ANDERSEN, Felicie, F.
发明人： KOCH, Jørn, Erland , STOUGAARD, Magnus , KNUDSEN, Birgitta, R. , ANDERSEN, Felicie, F.
IPC分类号： C12Q1/68 , C12Q1/533 , G01N33/50
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.

5. 发明申请

WO2013029631A2 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
标题翻译：微流控芯片检测
公开(公告)号：WO2013029631A2
公开(公告)日：2013-03-07
申请号：PCT/DK2012050327
申请日：2012-08-31
申请人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , ANDERSEN BIRGITTA RUTH , JUUL SISSEL , LEONG KAM , HO MEGAN YI-PING , ANDERSEN FELICIE F
发明人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , ANDERSEN BIRGITTA RUTH , JUUL SISSEL , LEONG KAM , HO MEGAN YI-PING , ANDERSEN FELICIE F
IPC分类号： G01N33/50
CPC分类号： C12Q1/6893 , C12Q1/00 , C12Q1/025 , C12Q1/04 , C12Q1/689 , G01N33/573
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.
摘要翻译：提供了微流体实施的检测酶，特别是DNA修饰酶的方法，以及检测细胞或表达所述酶的微生物的方法。通过提供被该酶特异性靶向的核酸底物来检测该酶。

6. 发明申请

WO2006108422A3 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108422A3
公开(公告)日：2006-12-14
申请号：PCT/DK2006050011
申请日：2006-04-10
申请人： UNIV AARHUS , KOCH JOERN , STOUGAARD MAGNUS , LOHMANN JAKOB SCHWALBE
发明人： KOCH JOERN , STOUGAARD MAGNUS , LOHMANN JAKOB SCHWALBE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6816 , C12Q2531/125 , C12Q2521/101 , C12Q2521/325 , C12Q2525/307 , C12Q2525/301 , C12Q2521/337 , C12Q2525/155 , C12Q2521/501
摘要： The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3'end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.
摘要翻译：本发明提供了使用滚环复制有效检测靶核酸的寡核苷酸和方法。一方面，本发明的寡核苷酸的特征在于它们可以在没有外部连接模板的情况下环化。另一方面，本发明的寡核苷酸的特征在于它们可以产生滚环复制所需的靶核酸的游离3'末端。本发明的寡核苷酸和检测方法是有用的，例如，作为研究工具和诊断。

7. 发明申请

WO2006108423A2 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108423A2
公开(公告)日：2006-10-19
申请号：PCT/DK2006/050012
申请日：2006-04-10
申请人： AARHUS UNIVERSITET , KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
发明人： KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6844 , C12Q2531/125 , C12Q2525/131 , C12Q2537/149 , C12Q2525/301 , C12Q2521/307
摘要： The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette.
摘要翻译：本发明涉及使用滚环复制制备寡核苷酸，其中合成的多聚寡核苷酸使用切口盒还原成单核苷酸。因此，本发明提供了一种生产寡核苷酸的方法，能够有效扩增长度至多为1000个核苷酸的寡核苷酸，并以高含量包含在切口盒中。

8. 发明申请

WO2006108422A2 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108422A2
公开(公告)日：2006-10-19
申请号：PCT/DK2006/050011
申请日：2006-04-10
申请人： AARHUS UNIVERSITET , KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
发明人： KOCH, Jørn , STOUGAARD, Magnus , LOHMANN, Jakob Schwalbe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6816 , C12Q2531/125 , C12Q2521/101 , C12Q2521/325 , C12Q2525/307 , C12Q2525/301 , C12Q2521/337 , C12Q2525/155 , C12Q2521/501
摘要： The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3'end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.
摘要翻译：本发明提供了使用滚动圆复制有效检测靶核酸的寡核苷酸和方法。在一个方面，本发明的寡核苷酸的特征在于它们可以在没有外部连接模板的情况下被循环。另一方面，本发明的寡核苷酸的特征在于它们可产生滚动圆复制所必需的靶核酸的游离的3'末端。本发明的寡核苷酸和检测方法是有用的。作为研究工具和诊断。

9. 发明申请

WO2006108423A3 METHODS FOR PRODUCTION OF OLIGONUCLEOTIDES 审中-公开
标题翻译：生产寡核苷酸的方法
公开(公告)号：WO2006108423A3
公开(公告)日：2007-01-25
申请号：PCT/DK2006050012
申请日：2006-04-10
申请人： UNIV AARHUS , KOCH JOERN , STOUGAARD MAGNUS , LOHMANN JAKOB SCHWALBE
发明人： KOCH JOERN , STOUGAARD MAGNUS , LOHMANN JAKOB SCHWALBE
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N15/10 , C12Q1/6844 , C12Q2531/125 , C12Q2525/131 , C12Q2537/149 , C12Q2525/301 , C12Q2521/307
摘要： The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette.
摘要翻译：本发明涉及使用滚环复制制备寡核苷酸，其中合成的多聚寡核苷酸使用切口盒还原成单核苷酸。因此，本发明提供了一种生产寡核苷酸的方法，能够有效扩增长度至多为1000个核苷酸的寡核苷酸，并以高含量包含在切口盒中。

10. 发明申请

WO2012149936A2 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 审中-公开
标题翻译：识别微生物和感染性疾病的检测系统
公开(公告)号：WO2012149936A2
公开(公告)日：2012-11-08
申请号：PCT/DK2012000056
申请日：2012-04-27
申请人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , KNUDSEN BIRGITTA R , ANDERSEN FELICIE F
发明人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , KNUDSEN BIRGITTA R , ANDERSEN FELICIE F
IPC分类号： C12Q1/68
CPC分类号： G01N33/56905 , C12Q1/533 , C12Q1/689 , C12Q1/6893 , C12Q2600/136 , G01N33/5695 , G01N2333/35 , G01N2333/445
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.
摘要翻译：公开了用于鉴定微生物或感染性疾病的方法，包括从诸如人，动物，植物，食物，水或土壤的来源获得合适的样品。所述方法还包括提供设计成与来自一种或多种微生物或感染因子的1型拓扑异构酶反应的定制的核酸底物，以及将所述底物与所述样品或来自样品的提取物或制剂如果所述微生物或感染剂存在于样品中，则底物被所述拓扑异构酶加工。最后，通过一系列标准分子生物学方法和读出系统中的一种或多种鉴定并可能量化经处理的底物。微生物和感染因子（包括但不限于恶性疟原虫和结核分枝杆菌）以单一或多重形式进行鉴定和潜在定量，可以通过鉴定潜在的有害生物来诊断，治疗或预防疾病。本披露还包括后续测试或继续监测以评估治疗或根除努力的反应。在另一方面，该技术使得能够测试旨在治疗或预防基于针对1型拓扑异构酶的药物的疾病的医疗或化学治疗。最后，用于所述目的所需的试剂和平台可以由松散的部件编制，或作为用户友好的试剂盒提供，潜在地可以对多种微生物或感染因子进行家庭测试。

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