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1. 发明公开

EP2751277A2 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
标题翻译： MIKROFLUIDSYSTEM ZUR ENZYMDETEKTION
公开(公告)号：EP2751277A2
公开(公告)日：2014-07-09
申请号：EP12758395.3
申请日：2012-08-31
申请人： Koch, Jørn, Erland , Stougaard, Magnus , Andersen, Birgitta Ruth , Juul, Sissel , Leong, Kam , Ho, Megan Yi-ping
发明人： ANDERSEN, Felicie F. , KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6893 , C12Q1/00 , C12Q1/025 , C12Q1/04 , C12Q1/689 , G01N33/573 , Y02A50/58
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.
摘要翻译：提供微流体实施的检测酶特别是DNA修饰酶的方法，以及检测细胞或表达所述酶的微生物的方法。通过提供由该酶特异性靶向的核酸底物来检测该酶。

2. 发明申请

WO2013029631A3 ENZYME DETECTION BY MICROFLUIDICS 审中-公开
公开(公告)号：WO2013029631A3
公开(公告)日：2013-03-07
申请号：PCT/DK2012/050327
申请日：2012-08-31
申请人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
发明人： KOCH, Jørn Erland , STOUGAARD, Magnus , ANDERSEN, Birgitta Ruth , JUUL, Sissel , LEONG, Kam , HO, Megan Yi-Ping , ANDERSEN, Felicie F.
IPC分类号： C12Q1/00
摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

3. 发明申请

WO2008148392A1 ENZYME ACTIVITY ASSAY USING ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：使用滚动圆放大器的酶活性测定
公开(公告)号：WO2008148392A1
公开(公告)日：2008-12-11
申请号：PCT/DK2008/050132
申请日：2008-06-03
申请人： IN SITU RCP A/S , LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørn Erland
发明人： LOHMANN, Jakob Schwalbe , STOUGAARD, Magnus , KOCH, Jørn Erland
IPC分类号： C12Q1/68 , C12Q1/25
CPC分类号： C12Q1/6844 , G01N33/57484 , G01N33/6896 , G01N2333/91245 , G01N2500/00 , C12Q2521/307 , C12Q2531/125 , C12Q2521/507 , C12Q2521/519
摘要： The present invention relates to an enzyme activity assay using rolling circle amplification for verifying that a sample contains the enzyme activity in question. Thus, the present invention pertains to a method for determining the presence or absence of one or more enzyme activities involved in circularising a non-circular oligonucleotide probe in a biological sample. Furthermore, the present invention concerns liquid compositions comprising one or more oligonucleotide probes. Within the scope of the present invention is also a composition comprising a liquid composition and a tissue sample, and solid support of one or more oligonucleotides of the present invention. Disclosed is also a microfluidic device with one or more compartments for performing rolling circle amplification events, and a method for correlating one or more rolling circle amplification events. Methods for testing the efficacy of a drug, for diagnosing or prognosing a disease, for treating a disease, or for treating prophylactically a disease is furthermore disclosed.
摘要翻译：本发明涉及使用滚环扩增用于验证样品含有所述酶活性的酶活性测定法。因此，本发明涉及用于确定参与生物样品中的非环状寡核苷酸探针的一个或多个酶活性的存在或不存在的方法。此外，本发明涉及包含一种或多种寡核苷酸探针的液体组合物。在本发明的范围内，也是包含液体组合物和组织样品以及本发明的一种或多种寡核苷酸的固体支持物的组合物。还公开了一种具有一个或多个用于执行滚环放大事件的隔间的微流体装置，以及用于使一个或多个滚动圆放大事件相关联的方法。还公开了用于测试药物的功效，用于诊断或预后疾病，用于治疗疾病或用于预防性治疗疾病的方法。

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