专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO2012149936A2 SYSTEM FOR IDENTIFICATION OF MICROORGANISM AND DETECTION OF INFECTIOUS DISORDER 审中-公开
标题翻译：识别微生物和感染性疾病的检测系统
公开(公告)号：WO2012149936A2
公开(公告)日：2012-11-08
申请号：PCT/DK2012000056
申请日：2012-04-27
申请人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , KNUDSEN BIRGITTA R , ANDERSEN FELICIE F
发明人： KOCH JOERN ERLAND , STOUGAARD MAGNUS , KNUDSEN BIRGITTA R , ANDERSEN FELICIE F
IPC分类号： C12Q1/68
CPC分类号： G01N33/56905 , C12Q1/533 , C12Q1/689 , C12Q1/6893 , C12Q2600/136 , G01N33/5695 , G01N2333/35 , G01N2333/445
摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Finally, processed substrates are identified and potentially quantified by one or more of a range of standard molecular biology methods and read-out systems. The identification and potential quantification of microorganisms and infectious agents, including but not limited to Plasmodium falciparum and Mycobacterium tuberculosis, in single or multiplexed formats enable diseases to be diagnosed, treated, or prevented through the identification of potentially harmful organisms. This disclosure includes also follow-up tests or continued monitoring to evaluate responses to treatment or eradication efforts. In a further aspect the technology enables the testing of medical or chemical treatments designed to cure or prevent diseases based upon drugs targeting type 1 topoisomerases. Finally, the reagents and platforms needed for said purposes can be compiled from loose parts or provided as user-friendly kits, potentially enabling home testing for a multitude of microorganisms or infectious agents.
摘要翻译：公开了用于鉴定微生物或感染性疾病的方法，包括从诸如人，动物，植物，食物，水或土壤的来源获得合适的样品。所述方法还包括提供设计成与来自一种或多种微生物或感染因子的1型拓扑异构酶反应的定制的核酸底物，以及将所述底物与所述样品或来自样品的提取物或制剂如果所述微生物或感染剂存在于样品中，则底物被所述拓扑异构酶加工。最后，通过一系列标准分子生物学方法和读出系统中的一种或多种鉴定并可能量化经处理的底物。微生物和感染因子（包括但不限于恶性疟原虫和结核分枝杆菌）以单一或多重形式进行鉴定和潜在定量，可以通过鉴定潜在的有害生物来诊断，治疗或预防疾病。本披露还包括后续测试或继续监测以评估治疗或根除努力的反应。在另一方面，该技术使得能够测试旨在治疗或预防基于针对1型拓扑异构酶的药物的疾病的医疗或化学治疗。最后，用于所述目的所需的试剂和平台可以由松散的部件编制，或作为用户友好的试剂盒提供，潜在地可以对多种微生物或感染因子进行家庭测试。

IPRDB

热门服务

关于我们

友情链接

联系方式