专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011073183A1 PROBES FOR GENOTYPING LOW-RISK-HPV 审中-公开
标题翻译：用于基因型低风险HPV的探针
公开(公告)号：WO2011073183A1
公开(公告)日：2011-06-23
申请号：PCT/EP2010/069614
申请日：2010-12-14
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , SCHMITT, Markus , PAWLITA, Michael
发明人： SCHMITT, Markus , PAWLITA, Michael
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： The current invention is concerned with a composition comprising at least one probe oligonucleotide each for the nucleotide sequences of the invention, said probe oligonucleotides specifically hybridizing to the sense strand or the antisense strand of said nucleotide sequences. Moreover, the present invention relates to a method for the identification of low-risk HPV types in a sample comprising the steps of a) contacting a sample with an amplification composition allowing amplification of at least one region of the HPV genome specifically hybridizing to at least one of the probe oligonucleotides of the current invention under conditions which allow for the amplification of polynucleotides and b) identifying low-risk HPV genotypes in said sample based on the amplified polynucleotides obtained in step a) by hybridizing the amplified polynucleotides with at least one labelled probe oligonucleotide of the current invention while said amplified polynucleotides are present in the same reaction container. The invention also relates to the use of a composition of the current invention for identifying low-risk HPV hi a sample and to a kit comprising the composition of the invention and / or adopted for carrying out the method of the invention and an instruction manual.
摘要翻译：本发明涉及包含本发明核苷酸序列的至少一种探针寡核苷酸的组合物，所述探针寡核苷酸与所述核苷酸序列的有义链或反义链特异性杂交。此外，本发明涉及一种用于鉴定样品中低风险HPV类型的方法，包括以下步骤：a）使样品与扩增组合物接触，所述扩增组合物允许至少一个HPV基因区域的扩增，特别是至少与本发明的探针寡核苷酸之一在允许扩增多核苷酸的条件下，以及b）基于步骤a）中获得的扩增多核苷酸鉴定所述样品中的低风险HPV基因型，通过将扩增的多核苷酸与至少一个标记的而所述扩增的多核苷酸存在于相同的反应容器中的本发明的探针寡核苷酸。本发明还涉及本发明的组合物在样品中鉴定低风险HPV的用途，以及包含本发明组合物和/或用于实施本发明方法和使用说明书的试剂盒的用途。

2. 发明申请

WO2012136712A1 MEANS AND METHODS FOR QUANTIFYING NUCLEIC ACIDS 审中-公开
标题翻译：用于定量核酸的方法和方法
公开(公告)号：WO2012136712A1
公开(公告)日：2012-10-11
申请号：PCT/EP2012/056178
申请日：2012-04-04
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , PAWLITA, Michael , SCHMITT, Markus
发明人： PAWLITA, Michael , SCHMITT, Markus
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6851 , C12Q1/6806 , C12Q1/708 , C12Q2545/114 , C12Q2600/16 , C12Q2527/143 , C12Q2537/143
摘要： The present invention relates to a method for comparing the amounts of a first and a second polynucleotide comprised in a sample, said method comprising the steps of a) amplifying said first and said second test polynucleotide in a test sample and a first and a second control polynucleotide in a reference sample, wherein said first and said second control polynucleotide is present in the reference sample in amounts sufficient to allow for generation of maximal amounts of amplification products of the said control polynucleotides, b) determining the amounts of the first and the second test amplification product of said first and said second polynucleotide and the first and the second control amplification product of said first and said second control polynucleotide, c) calculating (i) the ratio of the amounts of the first test amplification product and the first control amplification product and (ii) the ratio of the second test amplification product and the second control amplification product; and, d) comparing the ratios calculated in c) (i) and (ii), whereby the amount of the first test polynucleotide and the amount second test polynucleotide comprised in the test sample are compared. Furthermore, the present invention relates to a device for comparing the amounts of a first and a second polynucleotide comprised in a sample by the method of the present invention.
摘要翻译：本发明涉及一种用于比较样品中包含的第一和第二多核苷酸的量的方法，所述方法包括以下步骤：a）在测试样品中扩增所述第一和第二测试多核苷酸，以及第一和第二对照参考样品中的多核苷酸，其中所述第一和第二对照多核苷酸以足以允许产生所述对照多核苷酸的最大量的扩增产物的量存在于参照样品中，b）确定第一和第二所述第一和第二多核苷酸的测试扩增产物和所述第一和第二对照多核苷酸的第一和第二对照扩增产物，c）计算（i）第一测试扩增产物和第一对照扩增产物的量的比例产物和（ii）第二测试扩增产物和第二对照扩增的比率产品; 和d）比较在c）（i）和（ii）中计算的比例，由此比较测试样品中包含的第一测试多核苷酸和第二测试多核苷酸的量。此外，本发明涉及通过本发明的方法比较样品中包含的第一和第二多核苷酸的量的装置。

3. 发明申请

WO2011138234A3 DIAGNOSTIC TRANSCRIPT AND SPLICE PATTERNS OF HR-HPV IN DIFFERENT CERVICAL LESIONS 审中-公开
标题翻译：人乳头瘤病毒在不同宫颈癌中的诊断性病例和病理特征
公开(公告)号：WO2011138234A3
公开(公告)日：2013-11-07
申请号：PCT/EP2011056787
申请日：2011-04-28
申请人： DEUTSCHES KREBSFORSCH , SCHMITT MARKUS , GISSMANN LUTZ , PAWLITA MICHAEL , HOEFLER DANIELA
发明人： SCHMITT MARKUS , GISSMANN LUTZ , PAWLITA MICHAEL , HOEFLER DANIELA
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708 , C12Q1/6883
摘要： The present invention relates to a method for differentiating in a subject with HR-HPV between a severe form of HR-HPV infection and a mild form of HR-HPV infection. It further is concerned with a composition comprising a probe oligonucleotide mixture, a device, and a kit for use in conjunction with the method of the invention.
摘要翻译：本发明涉及一种在严重形式的HR-HPV感染与轻度形式的HR-HPV感染之间区分HR-HPV的受试者的方法。它还涉及包含探针寡核苷酸混合物，装置和与本发明的方法结合使用的试剂盒的组合物。

4. 发明申请

WO2011151335A1 HIGH-THROUGHPUT METHOD FOR DETERMINING THE PRESENCE OF PAPILLOMAVIRUS-NEUTRALIZING ANTIBODIES IN A SAMPLE 审中-公开
标题翻译：用于确定样品中斑马鱼中和抗体存在的高通量方法
公开(公告)号：WO2011151335A1
公开(公告)日：2011-12-08
申请号：PCT/EP2011/058966
申请日：2011-05-31
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , EUROPEAN MOLECULAR BIOLOGY LABORATORY , PAWLITA, Michael , RUBIO, Ivonne , MÜLLER, Martin , SEHR, Peter
发明人： PAWLITA, Michael , RUBIO, Ivonne , MÜLLER, Martin , SEHR, Peter
IPC分类号： C12Q1/66 , G01N33/68 , C12Q1/70 , C12Q1/68
CPC分类号： G01N33/6854 , C12Q1/66 , C12Q1/6813 , C12Q1/70
摘要： The present invention relates to a method for the determination of the presence of PV-neutralizing antibodies in a sample, comprising a) contacting said sample with infectious PV particles comprising a reporter gene, wherein the gene product of said reporter gene is secreted into the growth medium, b) contacting the PV particles from a) with host cells, and c) determining PV-neutralizing antibodies based on the amount of gene product from said reporter gene, wherein, preferably, a lower amount of said gene product as compared to a reference amount is indicative of the presence of PV-neutralizing antibodies. It further relates to a host cell strongly adhering to multi-cluster plates for use in a method for diagnosing anti- PV immunity comprising the method of the present invention.
摘要翻译：本发明涉及一种用于测定样品中PV中和抗体的存在的方法，包括a）使所述样品与包含报道基因的感染性PV颗粒接触，其中所述报告基因的基因产物分泌到生长中培养基，b）使来自a）的PV颗粒与宿主细胞接触，和c）基于来自所述报道基因的基因产物的量确定PV中和抗体，其中优选地，所述基因产物的量相对于参考量表明PV中和抗体的存在。本发明还涉及一种用于诊断抗PV免疫的方法的多簇板的宿主细胞，其包括本发明的方法。

5. 发明申请

WO2010052317A1 DIAGNOSTIC TRANSCRIPT AND SPLICE PATTERNS OF HPV16 IN DIFFERENT CERVICAL LESIONS 审中-公开
标题翻译： HPV16在不同宫颈癌中的诊断和病理特征
公开(公告)号：WO2010052317A1
公开(公告)日：2010-05-14
申请号：PCT/EP2009/064811
申请日：2009-11-09
申请人： DKFZ DEUTSCHES KREBSFORSCHUNGSZENTRUM, STIFTUNG DES ÖFFENTLICHEN RECHTS , SCHMITT, Markus , GISSMANN, Lutz , PAWLITA, Michael
发明人： SCHMITT, Markus , GISSMANN, Lutz , PAWLITA, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/708 , C12Q2545/113
摘要： The present invention relates to a method for differentiating in a subject with HPV 16 between (i) a severe form of HPV16 infection and (ii) a mild form of HPV16 infection based on determining the amount of a first gene product and a second gene product in a sample of a subject and calculating a ratio of the amount of said first gene product and the amount of said second gene product. Further envisaged by the present invention is a composition comprising an oligonucleotide mixture. Also envisaged by the present invention are a kit and a device adapted to carry out the method of the present invention.
摘要翻译：本发明涉及一种用于在（i）严重形式的HPV16感染和（ii）基于确定第一基因产物和第二基因产物的量的温和形式的HPV16感染之间的HPV16区分受试者的方法并且计算所述第一基因产物的量和所述第二基因产物的量的比例。本发明进一步设想的是包含寡核苷酸混合物的组合物。本发明还设想了适用于实施本发明方法的试剂盒和装置。

6. 发明申请

WO2012136682A1 VIRAL LOAD ASSESSMENT IN MULTIPLE HPV INFECTIONS AS PREDICTOR FOR THE PRESENCE OF CERVICAL LESIONS 审中-公开
标题翻译：多发性HPV感染的病毒载量评估作为宫颈癌患者的预防因素
公开(公告)号：WO2012136682A1
公开(公告)日：2012-10-11
申请号：PCT/EP2012/056119
申请日：2012-04-04
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , SCHMITT, Markus , PAWLITA, Michael
发明人： SCHMITT, Markus , PAWLITA, Michael
IPC分类号： C12Q1/68 , C12Q1/70 , B01L7/00
CPC分类号： C12Q1/708 , C12Q1/6851 , C12Q2600/112 , C12Q2537/143 , C12Q2537/16
摘要： The present invention is concerned with a method for differentiating in a subject infected with at least two high-risk (HR-) human papillomavirus (HPV) genotypes between a severe form of HPV infection and a mild form of HPV infection, comprising a) quantifying the genome numbers of each of said at least two HR-HPV-genotypes in a sample from said patient, b) comparing the genome number of the HR-HPV genotype with the highest genome number to at least one reference amount, and c) differentiating between (i) a severe form of HPV infection and (ii) a mild form of HPV infection. Furthermore, the current invention relates to a method for diagnosing a severe form of HPV infection in a subject comprising the method of the invention, as well as to oligonucleotides for use in said method for diagnosis and a device for use in said method.
摘要翻译：本发明涉及用于区分在严重形式的HPV感染和轻度形式的HPV感染之间感染至少两种高风险（HR））人乳头状瘤病毒（HPV）基因型的受试者的方法，其包括：a）量化所述患者的样品中所述至少两种HR-HPV基因型中的每一种的基因组数目，b）将HR-HPV基因型的基因组数目与最高基因组数目比较至至少一个参考量，以及c）区分在（i）严重形式的HPV感染和（ii）轻度形式的HPV感染之间。此外，本发明涉及用于诊断受试者中严重形式的HPV感染的方法，所述方法包括本发明的方法，以及用于所述诊断方法的寡核苷酸和用于所述方法的装置。

7. 发明申请

WO2009027403A1 COMPOSITION COMPRISING AN OLIGONUCLEOTIDE MIXTURE FOR IMPROVED DETECTION OF HUMAN PAPILLOMAVIRUS GENOTYPES 审中-公开
标题翻译：包含用于改善人类斑马鱼基因组检测的寡核苷酸混合物的组合物
公开(公告)号：WO2009027403A1
公开(公告)日：2009-03-05
申请号：PCT/EP2008/061156
申请日：2008-08-26
申请人： DKFZ DEUTSCHES KREBSFORSCHUNGSZENTRUM , SCHMITT, Markus , WATERBOER, Tim , PAWLITA, Michael
发明人： SCHMITT, Markus , WATERBOER, Tim , PAWLITA, Michael
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708 , C12Q2537/143 , C12Q2549/119
摘要： The present invention relates to a composition comprising an oligonucleotide mixture. Moreover, the present invention relates to the use of said oligonucleotide mixture for diagnosing different HPV genotypes in a sample of a subject. Further encompassed is a method for diagnosing different HPV genotypes in a sample of a subject and a kit carrying out said method.
摘要翻译：本发明涉及包含寡核苷酸混合物的组合物。此外，本发明涉及用于诊断受试者样品中不同HPV基因型的所述寡核苷酸混合物的用途。进一步包括用于诊断受试者样品中不同HPV基因型的方法和进行所述方法的试剂盒。

8. 发明申请

WO2003016329A2 GLYCOCONJUGATES OF SIALIC ACID DERIVATES, METHODS FOR THEIR PRODUCTION AND USE THEREOF 审中-公开
标题翻译：唾液酸衍生物的糖苷配基，其生产方法和用途
公开(公告)号：WO2003016329A2
公开(公告)日：2003-02-27
申请号：PCT/EP2002/009198
申请日：2002-08-17
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS , PAWLITA, Michael , OETKE, Cornelia , KEPPLER, Oliver , BROSSMER, Reinhard , HINDERLICH, Stephan , REUTTER, Werner
发明人： PAWLITA, Michael , OETKE, Cornelia , KEPPLER, Oliver , BROSSMER, Reinhard , HINDERLICH, Stephan , REUTTER, Werner
IPC分类号： C07K
CPC分类号： C07K14/4702 , A61K38/00
摘要： The present invention relates to glycoconjugates containing a sialic acid derivate of general formula (I) and wherein the sialic acid derivate of general formula (I) is conjugated to a mono-, di- or oligosaccharide with up to 40 glycosidically linked, optionally branched sugar residues representing furanose and/or pyranose rings, which are linked N- or O-glycosidically to the polypeptide. The sialic derivatives of general formula (I) are useful for producing pharmaceutical compositions for immunosuppression, cell protection, stimulation of hematopoesis regulation of hormonal secretion and hormonal activation.
摘要翻译：本发明涉及含有通式（I）的唾液酸衍生物的糖缀合物，并且其中通式（I）的唾液酸衍生物与单糖，二糖或寡糖缀合，至40个糖苷连接的任选支化的代表呋喃糖和/或吡喃糖环的糖残基，所述呋喃糖和/或吡喃糖环与N-或O-糖苷连接至多肽。通式（I）的唾液酸衍生物可用于制备用于免疫抑制，细胞保护，刺激血细胞生成调节激素分泌和激素活化的药物组合物。

9. 发明申请

WO1997002491A1 PROCESS FOR DETECTING SPECIFIC ANTIBODIES ACTING AGAINST HPV PROTEINS 审中-公开
标题翻译：检测方法特异性抗HPV蛋白抗体展望
公开(公告)号：WO1997002491A1
公开(公告)日：1997-01-23
申请号：PCT/DE1996001195
申请日：1996-07-04
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS , ZENTGRAF, Hanswalter , FREY, Manfred , VELHAGEN, Iris , MARTENS, Regina , MESCHEDE, Wolfgang , PAWLITA, Michael , BRASPENNING, Joris , TOMMASINO, Massimo
发明人： DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS
IPC分类号： G01N33/68
CPC分类号： C07K14/005 , C07K2319/00 , C12N2710/20022 , G01N33/54306 , G01N33/56983 , G01N33/6854 , G01N2333/025 , Y10S436/815 , Y10S530/826
摘要： The invention concerns a process for detecting specific antibodies in bodily fluids, which antibodies act against HPV proteins. The process involves the following steps: (I) incubating a native carrier material-bonded HPV protein with bodily fluids; and (II) reacting specific antibodies (a) bonded to the HPV protein either with marked antibodies (b) which are active against antibodies (a), or with unmarked antibodies (b) and then reacting the latter with marked antibodies (c) acting against antibodies (b). The invention further concerns a kit for carrying out this process.
摘要翻译：本发明涉及一种体液，其作用对HPV蛋白的抗体检测特异性抗体的过程。该过程包括以下步骤：（i）孵育与体液天然载体材料结合HPV蛋白; 和（II）使特异性抗体（a）中结合到HPV蛋白要么与标记抗体（B）的是对抗体（a）中，或与未标记的抗体（b）中，然后用标记的抗体发生特异性反应的后期（c）中起作用的活性针对抗体（b）。本发明还涉及一种用于实施该方法的试剂盒。

10. 发明申请

WO2011138234A2 DIAGNOSTIC TRANSCRIPT AND SPLICE PATTERNS OF HR-HPV IN DIFFERENT CERVICAL LESIONS 审中-公开
标题翻译：人乳头瘤病毒在不同宫颈癌中的诊断性病例和病理特征
公开(公告)号：WO2011138234A2
公开(公告)日：2011-11-10
申请号：PCT/EP2011/056787
申请日：2011-04-28
申请人： DEUTSCHES KREBSFORSCHUNGSZENTRUM , SCHMITT, Markus , GISSMANN, Lutz , PAWLITA, Michael , HÖFLER, Daniela
发明人： SCHMITT, Markus , GISSMANN, Lutz , PAWLITA, Michael , HÖFLER, Daniela
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708 , C12Q1/6883
摘要： The present invention relates to a method for differentiating in a subject with HR-HPV between a severe form of HR-HPV infection and a mild form of HR-HPV infection. It further is concerned with a composition comprising a probe oligonucleotide mixture, a device, and a kit for use in conjunction with the method of the invention.
摘要翻译：本发明涉及一种在严重形式的HR-HPV感染与轻度形式的HR-HPV感染之间区分HR-HPV的受试者的方法。它还涉及包含探针寡核苷酸混合物，装置和与本发明的方法结合使用的试剂盒的组合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式