The present invention relates to a method for comparing the amounts of a first and a second polynucleotide comprised in a sample, said method comprising the steps of a) amplifying said first and said second test polynucleotide in a test sample and a first and a second control polynucleotide in a reference sample, wherein said first and said second control polynucleotide is present in the reference sample in amounts sufficient to allow for generation of maximal amounts of amplification products of the said control polynucleotides, b) determining the amounts of the first and the second test amplification product of said first and said second polynucleotide and the first and the second control amplification product of said first and said second control polynucleotide, c) calculating (i) the ratio of the amounts of the first test amplification product and the first control amplification product and (ii) the ratio of the second test amplification product and the second control amplification product; and, d) comparing the ratios calculated in c) (i) and (ii), whereby the amount of the first test polynucleotide and the amount second test polynucleotide comprised in the test sample are compared. Furthermore, the present invention relates to a device for comparing the amounts of a first and a second polynucleotide comprised in a sample by the method of the present invention.