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    • 5. 发明授权
    • Process for the preparation of human leukocyte and human gamma interferon
    • 人类白细胞和人γ干扰素的制备方法
    • US4696899A
    • 1987-09-29
    • US681451
    • 1984-12-10
    • Miklos TothValeria EndreszIlona BeladiSandor Toth
    • Miklos TothValeria EndreszIlona BeladiSandor Toth
    • C12P21/00A61K35/14A61K38/21A61P43/00C07K1/14C07K1/22C07K14/52C07K14/555C07K14/56C07K14/57C07K16/00C12P21/02C12R1/91A61K45/02
    • C07K14/555Y10S435/811
    • The invention relates to a process for the preparation of .alpha.- and .gamma.-interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an .alpha.-interferon inducer and with a .gamma.-interferon inducer, which comprises producing .alpha.-interferon and .gamma.-interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/buffy coat/fraction of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with - or .beta.-interferon, contacting with an .alpha.-interferon inducer, separating the liquid containing the .alpha.-interferon from the cells, if desired recovering the -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising .alpha.-interferon from the cells and if desired recovering .alpha.-interferon and if desired making .alpha.-interferon free of .alpha.-interferon.The advantage of the process is that .alpha.- and .gamma.-interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available leukocytes.
    • 本发明涉及通过分离血液的血沉棕黄层级分,去除红细胞,将白细胞悬浮在合适的营养培养基中并用α-干扰素诱导剂和γ-干扰素诱导剂进行处理的方法来制备α-和γ-干扰素, 干扰素诱导剂,其包括通过预处理在分离白细胞/血沉棕黄层/血液部分之后获得的悬浮液,在相同的白细胞培养物中逐渐生成α-干扰素和γ-干扰素,去除红细胞并悬浮 在具有β-干扰素的合适营养培养基中的白细胞与α-干扰素诱导剂接触,如果需要,将含有α-干扰素的液体与细胞分离,如果需要,则回收 - 干扰素,洗涤并将细胞悬浮在合适的营养培养基 用有丝分裂剂处理,将含有α-干扰素的液体与细胞分离,并且如果需要,可以回收α-inter feron,如果需要,制造不含α-干扰素的α-干扰素。 该方法的优点是α-和γ-干扰素是在两个步骤中由相同的白细胞培养产生的。 这使得该方法更经济并能有效利用有限可用的白细胞。