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1. 发明授权

US5686132A Glucans having a cycle structure, and processes for preparing the same 失效
标题翻译：具有循环结构的葡聚糖及其制备方法
公开(公告)号：US5686132A
公开(公告)日：1997-11-11
申请号：US415152
申请日：1995-03-31
申请人： Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
发明人： Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
IPC分类号： C08B37/00 , C12P19/14 , A23G3/00 , A61K31/70 , C07H1/00
CPC分类号： C12P19/14 , C08B37/0009
摘要： The present invention provides glucans and derivatives thereof which are useful as raw materials in the starch processing industries, food and drink compositions, food additive compositions, and starch substitutes for biodegradable plastics, and processes for preparing the same. Particularly, it provides glucans and derivatives thereof having excellent properties such as higher solubility in water than conventional starches, lower viscosity and the glucans are not subject to retrogradation which is observed in conventional starches, and which are capable of preventing the retrogradation of starches.
摘要翻译：本发明提供了可用作淀粉加工工业中的原料的葡聚糖及其衍生物，食品和饮料组合物，食品添加剂组合物和用于可生物降解塑料的淀粉替代物，以及其制备方法。特别地，它提供具有优良性能的葡聚糖及其衍生物，例如比常规淀粉更高的在水中的溶解度，较低的粘度和葡聚糖不会在常规淀粉中观察到退化，并且能够防止淀粉的退化。

2. 发明授权

US07723090B2 Method of heat-stabilizing α-glucan phosphorylase (GP) 有权
标题翻译：热稳定α-葡聚糖磷酸化酶（GP）的方法
公开(公告)号：US07723090B2
公开(公告)日：2010-05-25
申请号：US12465135
申请日：2009-05-13
申请人： Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
发明人： Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
IPC分类号： C07H21/04 , C12N9/12
CPC分类号： C12N9/1051 , C12P19/02 , C12P19/04
摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
摘要翻译：提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有α-葡聚糖磷酸化酶的酶活性在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

3. 发明申请

US20130323799A1 METHOD FOR INDUSTRIALLY PRODUCING CYCLIC-STRUCTURE-CONTAINING BRANCHED GLUCAN 有权
标题翻译：用于工业生产包含结构分支葡萄糖的方法
公开(公告)号：US20130323799A1
公开(公告)日：2013-12-05
申请号：US13992205
申请日：2011-12-02
申请人： Takeshi Takaha , Kouji Odan , Michiyo Yanase , Iwao Kojima , Tsunehisa Akiyama
发明人： Takeshi Takaha , Kouji Odan , Michiyo Yanase , Iwao Kojima , Tsunehisa Akiyama
IPC分类号： C12P19/04
CPC分类号： C12P19/04 , C12P19/18 , C12Y204/01018
摘要： An object of the present invention is to provide a method for industrially producing a branched glucan having a cyclic structure. The method for producing a branched glucan having a cyclic structure comprises the steps of: (1) preparing a mixed liquid which contains a branching enzyme in which starch granules are suspended at a concentration of 5% by weight or more and 50% by weight or less, and allowing the branching enzyme to act on starch in the starch granules, wherein a temperature of the mixed liquid at the time of preparation is 0° C. or higher and not higher than the gelatinization starting temperature of the starch granule; and (2) elevating the temperature of the mixed liquid to 85° C. or higher and 129° C. or lower, wherein in the method, none of α-amylase, β-amylase, amyloglucosidase and a transglucosidase is added to the mixed liquid.
摘要翻译：本发明的目的是提供一种工业生产具有环状结构的支链葡聚糖的方法。制备具有环状结构的支链葡聚糖的方法包括以下步骤：（1）制备含有淀粉颗粒以5重量％以上至50重量％的浓度悬浮的分支酶的混合液，或较少，并且使分支酶对淀粉颗粒中的淀粉起作用，其中制备时的混合液的温度为0℃以上且不高于淀粉颗粒的糊化开始温度; 和（2）将混合液体的温度升高至85℃以上且129℃以下，其中，在该方法中，将混合液体中的α-淀粉酶，β-淀粉酶，淀粉葡糖苷酶和转葡糖苷酶均未添加到混合液体。

4. 发明申请

US20130302405A1 AMINO SUGAR-CONTAINING GLUCAN, METHOD FOR PRODUCING SAME, AND USE THEREOF 审中-公开
标题翻译：含有氨基糖的葡萄糖，其生产方法及其用途
公开(公告)号：US20130302405A1
公开(公告)日：2013-11-14
申请号：US13883509
申请日：2011-11-07
申请人： Takeshi Takaha , Akiko Kubo , Michiyo Yanase
发明人： Takeshi Takaha , Akiko Kubo , Michiyo Yanase
IPC分类号： A61K47/36 , C08B37/00
CPC分类号： A61K47/36 , A61K9/5161 , A61K47/59 , A61K47/6455 , A61K49/0054 , C08B31/00 , C08B35/00 , C08B37/0009 , C08B37/0012 , C08B37/0063 , C12N15/87 , C12P19/26
摘要： An object of the present invention is to provide a glucosamine-containing glucan, a modified product and a conjugate thereof. The glucosamine-containing glucan of the present invention is a glucosamine-containing glucan wherein the glucan has a plurality of non-reducing ends and at least one glucosamine residue is bound via an α-1,4-bond to each of two or more non-reducing ends of the branched α-1,4-glucan, but no glucosamine residue is present at a position other than the non-reducing ends of the branched α-1,4-glucan, wherein the degree of polymerization of the glucan is 15 or more and 4×105 or less. The glucosamine-containing glucan of the present invention can be provided by allowing an α-glucan phosphorylase to act on an aqueous solution comprising a branched α-1,4-glucan and glucosamine-1-phosphate.
摘要翻译：本发明的目的是提供含有葡糖胺的葡聚糖，其改性产物及其缀合物。本发明的含葡糖胺的葡聚糖是含葡糖胺的葡聚糖，其中葡聚糖具有多个非还原性末端，并且至少一个葡糖胺残基通过α-1,4-键与两个或多个非还原末端中的每一个结合， - 还原末端的支链α-1,4-葡聚糖，但在支链α-1,4-葡聚糖的非还原性末端以外的位置处不存在葡糖胺残基，其中葡聚糖的聚合度为 15以上且4×10 5以下。可以通过使α-葡聚糖磷酸化酶作用于含有支链α-1,4-葡聚糖和葡糖胺-1-磷酸的水溶液来提供本发明的含葡糖胺的葡聚糖。

5. 发明申请

US20120135063A1 GLUCURONIC ACID-CONTAINING GLUCAN, PROCESS FOR PRODUCTION OF SAME, AND USE OF SAME 有权
标题翻译：含葡萄糖的葡萄糖，其生产方法及其用途
公开(公告)号：US20120135063A1
公开(公告)日：2012-05-31
申请号：US13318958
申请日：2010-05-10
申请人： Junichi Kadokawa , Takeshi Takaha , Akiko Kubo , Michiyo Yanase , Kayo Hosoya
发明人： Junichi Kadokawa , Takeshi Takaha , Akiko Kubo , Michiyo Yanase , Kayo Hosoya
IPC分类号： A61K9/127 , C08B31/00 , C08B35/00 , C07H3/06 , C12P19/04 , A61K38/02 , A61K39/395 , A61K31/711 , A61K31/7105 , A61K31/713 , C08B37/00 , A61K47/36 , B82Y5/00
CPC分类号： A61K47/36 , A61K49/0002 , C08B37/0009 , C12N9/1051 , C12P19/04 , C12Y204/01001
摘要： An object of the present invention is to provide a uronic acid-containing glucan or a modified product thereof. The glucuronic acid-containing glucan of the present invention is a glucuronic acid-containing glucan in which a glucuronic acid residue is bound to at least one non-reducing end of a glucan, and the glucan is a branched α-1,4 glucan or a linear α-1,4 glucan. The glucuronic acid-containing glucan of the present invention can be provided by allowing α-glucan phosphorylase derived from Aquifex aeolicus VF5 to act on glucuronic acid-1-phosphate to thereby transfer a glucuronic acid residue to the non-reducing end of the receptor glucan.
摘要翻译：本发明的目的是提供一种含糖醛酸的葡聚糖或其改性产物。本发明的含葡萄糖醛酸的葡聚糖是葡萄糖醛酸的葡聚糖，其中葡萄糖醛酸残基与葡聚糖的至少一个非还原性末端结合，葡聚糖是支链α-1,4葡聚糖或线性α-1,4葡聚糖。本发明的含葡萄糖醛酸的葡聚糖可以通过使来自Aquifex aeolicus VF5的α-葡聚糖磷酸化酶作用于葡萄糖醛酸-1-磷酸酯，从而将葡萄糖醛酸残基转移到受体葡聚糖的非还原末端来提供。

6. 发明申请

US20080206822A1 Method for Improving the Thermostability of Sucrose Phosphorylase (Sp) 有权
标题翻译：提高蔗糖磷酸化酶（Sp）热稳定性的方法
公开(公告)号：US20080206822A1
公开(公告)日：2008-08-28
申请号：US10566224
申请日：2004-08-31
申请人： Kazutoshi Fujii , Masae Iiboshi , Michiyo Yanase , Hiroki Takata , Takeshi Takaha , Takashi Kuriki
发明人： Kazutoshi Fujii , Masae Iiboshi , Michiyo Yanase , Hiroki Takata , Takeshi Takaha , Takashi Kuriki
IPC分类号： C12P19/12 , C12N9/10 , C07H21/04 , C12N5/00 , C12N15/00
CPC分类号： C12N9/1051
摘要： A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.
摘要翻译：提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶（SP）和具有改善的热稳定性的制备SP的方法。具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基，在选自对应于位置14的位置，对应于位置29的位置和对应于位置的位置的至少一个位置基序序列1中的位置44; 对应于位置7的位置，对应于位置19的位置，对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置，并且其中在55℃下加热在20mM Tris缓冲液（pH7.0）中具有改善的热稳定性的SP后，SP的酶活性在37℃下具有改善的热稳定性。 20分钟，在加热前在37℃下具有改善的热稳定性的SP的酶活性的20％或更多。

7. 发明申请

US20060275875A1 Method of heat-stabilizing a-glucan phosphorylase(gp) 失效
标题翻译： α-葡聚糖磷酸化酶热稳定方法（gp）
公开(公告)号：US20060275875A1
公开(公告)日：2006-12-07
申请号：US10560491
申请日：2004-06-15
申请人： Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
发明人： Michiyo Yanase , Hiroki Takata , Kazutoshi Fujii , Takeshi Takaha , Takashi Kuriki
IPC分类号： C12P19/04 , C07H21/04 , C12P21/06 , C12N9/12 , C12N9/16
CPC分类号： C12N9/1051 , C12P19/02 , C12P19/04
摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
摘要翻译：提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

8. 发明授权

US06248566B1 Glucan having cyclic structure and method for producing the same 失效
标题翻译：具有环状结构的葡聚糖及其制造方法
公开(公告)号：US06248566B1
公开(公告)日：2001-06-19
申请号：US08528026
申请日：1995-09-13
申请人： Tadayuki Imanaka , Yoshinobu Terada , Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
发明人： Tadayuki Imanaka , Yoshinobu Terada , Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
IPC分类号： C12P1900
CPC分类号： C08B37/0009 , C08B37/0015 , C09J105/00 , C12P19/14 , C12P19/16 , C12P19/18
摘要： Glucan with a degree of polymerization of 50 or more includes an inner branched cyclic structure portion and an outer branched structure portion, and methods for producing the same.
摘要翻译：聚合度为50以上的葡聚糖包括内分支环结构部分和外分支结构部分及其制造方法。

9. 发明授权

US5827697A Process for preparing glucans having a cyclic structure 失效
标题翻译：制备具有环状结构的葡聚糖的方法
公开(公告)号：US5827697A
公开(公告)日：1998-10-27
申请号：US560739
申请日：1995-11-20
申请人： Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
发明人： Takeshi Takaha , Michiyo Yanase , Shigetaka Okada , Hiroki Takata , Hiroyasu Nakamura , Kazutoshi Fujii
IPC分类号： C08B37/00 , C09J105/00 , C12P19/14 , C12P19/16 , C12P19/18 , C12P19/04
CPC分类号： C12P19/16 , C08B37/0009 , C08B37/0015 , C09J105/00 , C12P19/14 , C12P19/18
摘要： The present invention provides glucans and derivatives thereof which are useful as raw materials in the starch processing industries, food and drink compositions, food additive compositions, and starch substitutes for biodegradable plastics, and processes for preparing the same. Particularly, it provides glucans and derivatives thereof having excellent properties such as higher solubility in water than conventional starches, lower viscosity and the glucans are not subject to retrogradation which is observed in conventional starches, and which are capable of preventing the retrogradation of starches.
摘要翻译：本发明提供了可用作淀粉加工工业中的原料的葡聚糖及其衍生物，食品和饮料组合物，食品添加剂组合物和用于可生物降解塑料的淀粉替代物，以及其制备方法。特别地，它提供具有优良性能的葡聚糖及其衍生物，例如比常规淀粉更高的在水中的溶解度，较低的粘度和葡聚糖不会在常规淀粉中观察到退化，并且能够防止淀粉的退化。

10. 发明授权

US07229801B2 Production method and preparation method of glucans 有权
标题翻译：葡聚糖的制备方法和制备方法
公开(公告)号：US07229801B2
公开(公告)日：2007-06-12
申请号：US10475943
申请日：2002-05-27
申请人： Kazutoshi Fujii , Yoshinobu Terada , Michiyo Yanase , Koji Odan , Hiroki Takata , Takeshi Takaha , Takashi Kuriki , Shigetaka Okada
发明人： Kazutoshi Fujii , Yoshinobu Terada , Michiyo Yanase , Koji Odan , Hiroki Takata , Takeshi Takaha , Takashi Kuriki , Shigetaka Okada
IPC分类号： C12P19/60 , C12N9/10
CPC分类号： C08B37/0009 , C08B30/20 , C08B33/00 , C12P19/18
摘要： A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.
摘要翻译：制备葡聚糖的第一种方法包括使含有蔗糖，底漆，无机磷酸盐或葡萄糖-1-磷酸，蔗糖磷酸化酶和葡聚糖磷酸化酶的反应溶液反应生成葡聚糖的步骤。从反应开始到反应结束的反应溶液的蔗糖磷酸酯比例的最大值不超过约17。第二种葡聚糖的制备方法包括以下步骤：使含有蔗糖的反应溶液，底物，无机磷酸盐或葡萄糖-1-磷酸盐，蔗糖磷酸化酶和葡聚糖磷酸化酶反应产生葡聚糖。反应在约40℃至约70℃的温度下进行。

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