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    • 1. 发明授权
    • Switching power supply device
    • 开关电源装置
    • US09055699B2
    • 2015-06-09
    • US13552911
    • 2012-07-19
    • Tomokazu OsatoKazutoshi Fujii
    • Tomokazu OsatoKazutoshi Fujii
    • H05K7/20
    • H05K7/20909
    • A switching power supply device includes a case, a board that has circuit parts containing a switching element mounted thereon and is accommodated in the case, and a heat sink that is provided in the case so as to partition the inside of the case into a part mount chamber having the board accommodated therein and an air flow path through which air flows. The switching element of the circuit parts is mounted on the board while brought into contact with a surface of the heat sink that faces the part mount chamber, and cooled through the heat sink by the air flowing in the air flow path.
    • 开关电源装置包括壳体,具有安装在其上并且容纳在壳体中的开关元件的电路部分的电路板,以及设置在壳体中以将壳体内部分隔成部分的散热器 其中容纳有板的安装室和空气流过的空气流动路径。 电路部件的开关元件安装在板上,同时与散热器的面向部件安装室的表面接触,并且通过在空气流动路径中流动的空气通过散热器而被冷却。
    • 2. 发明授权
    • Method for improving the thermostability of sucrose phosphorylase (SP)
    • 提高蔗糖磷酸化酶(SP)热稳定性的方法
    • US07968309B2
    • 2011-06-28
    • US10566224
    • 2004-08-31
    • Kazutoshi FujiiMasae IiboshiMichiyo YanaseHiroki TakataTakeshi TakahaTakashi Kuriki
    • Kazutoshi FujiiMasae IiboshiMichiyo YanaseHiroki TakataTakeshi TakahaTakashi Kuriki
    • C12Q1/48C12N9/12
    • C12N9/1051
    • A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.
    • 提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶(SP)和具有改善的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基,在选自对应于位置14的位置,对应于位置29的位置和对应于 基序序列1中的位置44; 对应于位置7的位置,对应于位置19的位置,对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置,并且其中在55℃下加热在20mM Tris缓冲液(pH7.0)中具有改善的热稳定性的SP后,SP的酶活性在37℃下具有改善的热稳定性。 20分钟,在加热前在37℃下具有改善的热稳定性的SP的酶活性的20%或更多。
    • 3. 发明申请
    • METHOD OF HEAT-STABILIZING alpha-GLUCAN PHOSPHORYLASE (GP)
    • 热稳定α葡萄糖磷酸酶(GP)的方法
    • US20100047891A1
    • 2010-02-25
    • US12465135
    • 2009-05-13
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • C12N9/10
    • C12N9/1051C12P19/02C12P19/04
    • An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
    • 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶,以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物,具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基,所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4,对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置,并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液(pH7.7)中加热10分钟后,在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20%或更多 C.加热前。
    • 4. 发明授权
    • Method of heat-stabilizing α-glucan phosphorylase(GP)
    • 热稳定α-葡聚糖磷酸化酶(GP)的方法
    • US07569377B2
    • 2009-08-04
    • US10560491
    • 2004-06-15
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • C12N9/12C07H21/04
    • C12N9/1051C12P19/02C12P19/04
    • An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
    • 提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶,以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物,具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基,所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4,对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置,并且其中具有 在60℃下在20mM柠檬酸盐缓冲液(pH7.7)中加热10分钟后,在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20%或更多 C.加热前。
    • 6. 发明授权
    • Method of heat-stabilizing α-glucan phosphorylase (GP)
    • 热稳定α-葡聚糖磷酸化酶(GP)的方法
    • US07723090B2
    • 2010-05-25
    • US12465135
    • 2009-05-13
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • C07H21/04C12N9/12
    • C12N9/1051C12P19/02C12P19/04
    • An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
    • 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶,以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物,具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基,所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4,对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置,并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液(pH7.7)中加热10分钟后,在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20%或更多 C.加热前。
    • 8. 发明申请
    • Method for Improving the Thermostability of Sucrose Phosphorylase (Sp)
    • 提高蔗糖磷酸化酶(Sp)热稳定性的方法
    • US20080206822A1
    • 2008-08-28
    • US10566224
    • 2004-08-31
    • Kazutoshi FujiiMasae IiboshiMichiyo YanaseHiroki TakataTakeshi TakahaTakashi Kuriki
    • Kazutoshi FujiiMasae IiboshiMichiyo YanaseHiroki TakataTakeshi TakahaTakashi Kuriki
    • C12P19/12C12N9/10C07H21/04C12N5/00C12N15/00
    • C12N9/1051
    • A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the SP having improved thermostability at 37° C. before heating.
    • 提供了通过改善天然SP获得的具有改进的热稳定性的蔗糖磷酸化酶(SP)和具有改善的热稳定性的制备SP的方法。 具有改善的热稳定性的SP具有与天然蔗糖磷酸化酶不同的氨基酸残基,在选自对应于位置14的位置,对应于位置29的位置和对应于位置的位置的至少一个位置 基序序列1中的位置44; 对应于位置7的位置,对应于位置19的位置,对应于位置23的位置和对应于图案序列2中的位置34的位置; 和与基序序列3中的位置19相对应的位置,并且其中在55℃下加热在20mM Tris缓冲液(pH7.0)中具有改善的热稳定性的SP后,SP的酶活性在37℃下具有改善的热稳定性。 20分钟,在加热前在37℃下具有改善的热稳定性的SP的酶活性的20%或更多。
    • 9. 发明申请
    • Method of heat-stabilizing a-glucan phosphorylase(gp)
    • α-葡聚糖磷酸化酶热稳定方法(gp)
    • US20060275875A1
    • 2006-12-07
    • US10560491
    • 2004-06-15
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • Michiyo YanaseHiroki TakataKazutoshi FujiiTakeshi TakahaTakashi Kuriki
    • C12P19/04C07H21/04C12P21/06C12N9/12C12N9/16
    • C12N9/1051C12P19/02C12P19/04
    • An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.
    • 提供了通过改良天然α-葡聚糖磷酸化酶获得的具有改善的热稳定性的α-葡聚糖磷酸化酶,以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物,具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基,所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4,对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置,并且其中具有 在60℃下在20mM柠檬酸盐缓冲液(pH7.7)中加热10分钟后,在37℃下改善的热稳定性是在37℃下具有改善的热稳定性的α-葡聚糖磷酸化酶的酶活性的20%或更多 C.加热前。