专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09777320B2 Molecular adaptors for dye conjugates 有权
公开(公告)号：US09777320B2
公开(公告)日：2017-10-03
申请号：US13218439
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
发明人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
IPC分类号： C12Q1/68 , C09B23/10 , C07D209/14 , C07D401/14 , C07D405/14 , G01N33/58 , C09B23/00 , C09B23/06 , C09B23/08 , C07H19/10 , C09B23/01 , C07K5/09 , C07K5/11
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an adaptor. An adaptor can be a component of a linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.

2. 发明授权

US08388982B2 Photo-induced damage mitigating agents and preparation and methods of use thereof 有权
标题翻译：光诱导的损伤缓解剂及其制备方法及其使用方法
公开(公告)号：US08388982B2
公开(公告)日：2013-03-05
申请号：US12622375
申请日：2009-11-19
申请人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
发明人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
IPC分类号： A61K9/00 , A61K8/00 , A61F13/00 , C12Q1/68 , C12Q1/48 , C12Q1/02
CPC分类号： C07C49/255 , G01N21/6428 , G01N33/5306 , G01N2021/6432
摘要： Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
摘要翻译：用于通过向反应混合物中加入一种或多种光诱导的损伤缓解剂来减少和/或防止一种或多种反应物在照明分析反应中的光诱导损伤的组合物，装置，系统和方法，并允许反应进行这个时间小于光致伤害阈值周期。

3. 发明授权

US08252911B2 Compositions and methods for use in analytical reactions 有权
标题翻译：用于分析反应的组合物和方法
公开(公告)号：US08252911B2
公开(公告)日：2012-08-28
申请号：US12370472
申请日：2009-02-12
申请人： Keith Bjornson , Arek Bibillo , Lubomir Sebo
发明人： Keith Bjornson , Arek Bibillo , Lubomir Sebo
IPC分类号： C07H21/02 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6874 , C12Q2527/127 , C12Q2527/113
摘要： Compositions, methods, substrates and systems for use in analysis of single molecule reactions and particularly single molecule nucleic acid sequence analysis. Compositions that include non-reactive, distinguishable or undetectable competitive substrates for the reaction system of interest are provided, as well as their use in systems and substrates for such applications, such compounds typically preferably polyphosphate chains or analogous structures.
摘要翻译：用于分析单分子反应，特别是单分子核酸序列分析的组合物，方法，底物和系统。提供了包含用于感兴趣的反应体系的非反应性，可区分或不可检测的竞争性底物的组合物，以及它们在用于这些应用的系统和底物中的用途，这些化合物通常优选多磷酸酯链或类似结构。

4. 发明申请

US20120077189A1 SCAFFOLD-BASED POLYMERASE ENZYME SUBSTRATES 有权
标题翻译：基于SCAFFOLD的聚合酶酶基质
公开(公告)号：US20120077189A1
公开(公告)日：2012-03-29
申请号：US13218382
申请日：2011-08-25
申请人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
发明人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
IPC分类号： C12Q1/68 , C07H19/207
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
摘要翻译：本发明提供了一类新型的基于支架的标记聚合酶底物。聚合酶底物具有连接有荧光染料部分和核苷磷酸酯多糖的多价核心或支架。聚合酶底物具有多个荧光染料体系和/或多个核苷磷酸酯部分。优选的多价核心包括三官能六元芳族部分。本发明还提供了使用基于支架的标记聚合酶底物的测序方法和试剂盒。

5. 发明申请

US20070088163A1 Modulators of calcitonin and amylin activity 审中-公开
标题翻译：降钙素和胰岛淀粉酶活性的调节剂
公开(公告)号：US20070088163A1
公开(公告)日：2007-04-19
申请号：US10571031
申请日：2004-09-10
申请人： Kent Pryor , Eddine Saiah , Jeffrey Kahl , Nancy Delaet , Edward Roberts , Jan Urban , Lubomir Sebo , Christopher Lum , Hiroshi Nakanishi
发明人： Kent Pryor , Eddine Saiah , Jeffrey Kahl , Nancy Delaet , Edward Roberts , Jan Urban , Lubomir Sebo , Christopher Lum , Hiroshi Nakanishi
IPC分类号： C07D471/02 , C07D215/16
CPC分类号： C07D471/04 , C07D405/12
摘要： In various aspects, the present invention relates to novel compounds, which modulate calcitonin and amylin receptor activity; to processes for the preparation of such compounds; and to pharmaceutical compositions including such compounds. Compounds of the invention are useful as calcitonin and/or amylin agonists and in the treatment of bone disease such as osteoporosis, Paget's disease, hypercalcemia, and in the treatment of metabolic diseases, such as insulin-requiring states.
摘要翻译：在各个方面，本发明涉及调节降钙素和胰岛淀粉样多肽受体活性的新化合物; 制备这些化合物的方法; 以及包含这些化合物的药物组合物。本发明的化合物可用作降钙素和/或胰岛淀粉样多肽激动剂和治疗骨疾病如骨质疏松症，佩吉特氏病，高钙血症，以及治疗代谢疾病如胰岛素需求状态。

6. 发明申请

US20110212437A1 SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS 有权
标题翻译：单分子序列与两个不同的化学步骤
公开(公告)号：US20110212437A1
公开(公告)日：2011-09-01
申请号：US13022926
申请日：2011-02-08
申请人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
发明人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
IPC分类号： C12Q1/68 , C12M1/34 , C07H19/04
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/11 , C07H19/20 , C07H19/213 , C12Q1/6874 , C12Q2521/301 , C12Q2525/186 , C12Q2563/107 , C12Q2537/157 , C12Q2521/319 , C12Q2521/525
摘要： Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.
摘要翻译：提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。

7. 发明授权

US08906612B2 Scaffold-based polymerase enzyme substrates 有权
标题翻译：基于脚手架的聚合酶底物
公开(公告)号：US08906612B2
公开(公告)日：2014-12-09
申请号：US13218382
申请日：2011-08-25
申请人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
发明人： Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04 , C09B23/06 , C07D209/14 , C09B23/01 , C09B23/08 , G01N33/58 , C07D401/14 , C07D405/14
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
摘要翻译：本发明提供了一类新型的基于支架的标记聚合酶底物。聚合酶底物具有连接有荧光染料部分和核苷磷酸酯多糖的多价核心或支架。聚合酶底物具有多个荧光染料体系和/或多个核苷磷酸酯部分。优选的多价核心包括三官能六元芳族部分。本发明还提供了使用基于支架的标记聚合酶底物的测序方法和试剂盒。

8. 发明授权

US08603741B2 Single molecule sequencing with two distinct chemistry steps 有权
标题翻译：具有两个不同化学步骤的单分子测序
公开(公告)号：US08603741B2
公开(公告)日：2013-12-10
申请号：US13022926
申请日：2011-02-08
申请人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
发明人： Robin Emig , Lei Jia , Jeremiah Hanes , Lubomir Sebo
IPC分类号： C12Q1/68 , C07H19/04
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/11 , C07H19/20 , C07H19/213 , C12Q1/6874 , C12Q2521/301 , C12Q2525/186 , C12Q2563/107 , C12Q2537/157 , C12Q2521/319 , C12Q2521/525
摘要： Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.
摘要翻译：提供了用于核酸测序的方法，组合物和系统，其中连续掺入核苷酸使用两个不同的化学步骤。在选择性切割活性的存在下，可以将多个在其3'羟基上具有标记的离去基团的核苷酸类似物依次加入到生长链中，该选择性裂解活性优选在其引入后优先切割3'羟基离去基团。选择性切割剂可以包含核酸外切酶活性，并且核酸外切酶活性可以是聚合酶相关的外切核酸酶活性。在可切割聚磷酸酯部分和3'羟基离去基团上具有标记的核苷酸类似物可以提供核苷酸类似物掺入特征的信号。具有照明光学器件，收集光学元件和底物的系统在标记被并入生长的核酸链中观察信号，允许对模板核酸进行测序。

9. 发明申请

US20120058473A1 Molecular Adaptors for Dye Conjugates 有权
标题翻译：染料缀合物的分子适配器
公开(公告)号：US20120058473A1
公开(公告)日：2012-03-08
申请号：US13218439
申请日：2011-08-25
申请人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
发明人： Stephen Yue , Gene Shen , Louis Brogley , Lubomir Sebo , Jeffrey Wegener
IPC分类号： C12Q1/68 , C07K17/10 , C07H19/207 , C07H19/10
CPC分类号： C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
摘要： In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an adaptor. An adaptor can be a component of a linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.
摘要翻译：在各种实施方案中，本发明提供了通过衔接剂部分与另一种物质连接的荧光染料。在一个示例性实施方案中，染料通过适配器与多磷酸核酸连接。适配器可以是链接器的组件。这些缀合物可用于单分子DNA测序和其他应用。在各种实施方案中，染料部分是花青染料。高度带电的花青染料，例如包括多种磺酸盐，烷基磺酸盐，羧酸盐和/或烷基羧酸盐部分的花青染料是本发明化合物中使用的花青染料的实例。

10. 发明申请

US20100136592A1 Photo-Induced Damage Mitigating Agents and Preparation and Methods of Use Thereof 有权
标题翻译：光诱导减伤剂及其制备及使用方法
公开(公告)号：US20100136592A1
公开(公告)日：2010-06-03
申请号：US12622375
申请日：2009-11-19
申请人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
发明人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
IPC分类号： C12Q1/48 , C12N9/00 , C12N11/00 , C12N9/12 , C12Q1/00 , C07H19/04 , C12N9/96 , C12M1/34 , C09K15/08
CPC分类号： C07C49/255 , G01N21/6428 , G01N33/5306 , G01N2021/6432
摘要： Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
摘要翻译：用于通过向反应混合物中加入一种或多种光诱导的损伤缓解剂来减少和/或防止一种或多种反应物在照明分析反应中的光诱导损伤的组合物，装置，系统和方法，并允许反应进行这个时间小于光致伤害阈值期。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式