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1. 发明申请

US20090311745A1 GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权
公开(公告)号：US20090311745A1
公开(公告)日：2009-12-17
申请号：US12300275
申请日：2007-05-03
申请人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
发明人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
IPC分类号： C12P21/00 , C12P7/62 , C12P7/40 , C12P7/02 , C12N15/00 , C12N15/74 , C12N9/18 , C12N1/21 , C12N15/63 , C07K16/40 , C07H21/00
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.

2. 发明授权

US08772007B2 Perhydrolase 有权
标题翻译：过水解酶
公开(公告)号：US08772007B2
公开(公告)日：2014-07-08
申请号：US10581014
申请日：2004-12-03
申请人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/14 , C12N9/18 , C12P21/06 , C12N1/20 , C12N15/00 , C11D3/386 , C11D3/00 , C07H21/04
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译：本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。本发明特别用于涉及清洗，漂白和消毒的应用中。

3. 发明授权

US07872100B2 Nitrile hydratases from metagenome libraries 有权
标题翻译：来自宏基因组文库的腈水合酶
公开(公告)号：US07872100B2
公开(公告)日：2011-01-18
申请号：US10593357
申请日：2005-03-10
申请人： Stefan Verseck , Klaus Liebeton , Jurgen Eck
发明人： Stefan Verseck , Klaus Liebeton , Jurgen Eck
IPC分类号： C07K14/00 , C07K17/00 , C07H21/02 , C07H21/04
CPC分类号： C12N9/88 , C12Y402/01084
摘要： The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.
摘要翻译：本发明涉及新型腈水合酶的制备。这些后者优选通过基于PCR的筛选从使用特殊简并引物的宏基因组DNA文库中获得来自非可培养生物体。

4. 发明申请

US20100047863A1 GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY 失效
标题翻译：具有广泛基质特征的新型强化微生物氮化物组
公开(公告)号：US20100047863A1
公开(公告)日：2010-02-25
申请号：US12296057
申请日：2007-04-05
申请人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
发明人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
IPC分类号： C12P21/06 , C12P13/02 , C12N15/74 , C12N1/21 , C12N15/63 , C07K14/00 , C07K16/00 , C07H21/04
CPC分类号： C12N9/88 , C12P13/02 , C12P41/006
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites.
摘要翻译：本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化亚硝酸盐

5. 发明申请

US20080171389A1 Nitrile Hydratases From Metagenome Libraries 有权
标题翻译：来自Metagenome图书馆的腈水合酶
公开(公告)号：US20080171389A1
公开(公告)日：2008-07-17
申请号：US10593357
申请日：2005-03-10
申请人： Stefan Verseck , Klaus Liebeton , Jurgen Eck
发明人： Stefan Verseck , Klaus Liebeton , Jurgen Eck
IPC分类号： C12N15/00 , C07H21/04 , C07K14/00 , C12N9/00
CPC分类号： C12N9/88 , C12Y402/01084
摘要： The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.
摘要翻译：本发明涉及新型腈水合酶的制备。这些后者优选通过基于PCR的筛选从使用特殊简并引物的宏基因组DNA文库中获得来自非可培养生物体。

6. 发明申请

US20070128689A1 Reca-negative and rhab-negative microorganism 审中-公开
标题翻译： Reca阴性和Rhab阴性微生物
公开(公告)号：US20070128689A1
公开(公告)日：2007-06-07
申请号：US10492939
申请日：2002-11-11
申请人： Oliver May , Klaus Liebeton , Jurgen Eck
发明人： Oliver May , Klaus Liebeton , Jurgen Eck
IPC分类号： C12P21/06 , C12N9/16 , C12N15/74 , C07H21/04 , C12N1/21
CPC分类号： C12R1/19 , C12N15/68 , C12N15/70 , C12P13/04 , C12P21/02 , C12P41/006
摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-in-ducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, ex-pression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.
摘要翻译：本发明描述了专门用作用于鼠李糖苷可启动子的表达系统的新微生物。由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并且具有较高遗传稳定性的预压系统。

7. 发明授权

US09193961B2 Polynucleotides encoding enantioselective carboxylesterases and methods of making same 有权
标题翻译：编码对映选择性羧酸酯酶的多核苷酸及其制备方法
公开(公告)号：US09193961B2
公开(公告)日：2015-11-24
申请号：US12300275
申请日：2007-05-03
申请人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
发明人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
IPC分类号： A61K38/00 , C12N9/18
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C.3.1.1.1] activity.
摘要翻译：本发明涉及编码具有羧酸酯酶[E.C.3.1.1.1]活性的酶的多核苷酸。

8. 发明授权

US08470576B2 Group of novel enantioselective microbial nitrile hydratases with broad substrate specificity 失效
标题翻译：一组具有广泛底物特异性的新型对映选择性微生物腈水合酶
公开(公告)号：US08470576B2
公开(公告)日：2013-06-25
申请号：US12296057
申请日：2007-04-05
申请人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
发明人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/06 , C12P21/04 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/88 , C12P13/02 , C12P41/006
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.
摘要翻译：本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化腈类

9. 发明申请

US20080145353A1 Perhydrolase 有权
标题翻译：过水解酶
公开(公告)号：US20080145353A1
公开(公告)日：2008-06-19
申请号：US10581014
申请日：2004-12-03
申请人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
IPC分类号： A01N63/00 , C12N9/14 , C07K2/00 , C07H21/04 , C12Q1/68 , C12N15/63 , C12N1/21 , C11D3/20
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译：本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。本发明特别用于涉及清洗，漂白和消毒的应用中。

10. 发明申请

US20080102496A1 Expression of Nitrile Hydratases in a Two-Vector Expression System 审中-公开
标题翻译：腈水合酶在双向表达系统中的表达
公开(公告)号：US20080102496A1
公开(公告)日：2008-05-01
申请号：US10593362
申请日：2005-02-18
申请人： Stefan Verseck , Steffen Osswald , Wai-Yee Phong , Klaus Liebeton , Jurgen Eck
发明人： Stefan Verseck , Steffen Osswald , Wai-Yee Phong , Klaus Liebeton , Jurgen Eck
IPC分类号： C12N1/20 , C12N15/00 , C12N15/64 , C12N9/88 , C12P7/40
CPC分类号： C12N15/70 , C12N9/88
摘要： The present invention relates to a novel system for expressing nitrile hydratase. In this system, the nitrile hydratases, which are composed of subunits, are formed such that the respective subunits are located on different plasmids and are expressed simultaneously in E. coli.
摘要翻译：本发明涉及一种表达腈水合酶的新型体系。在该系统中，形成由亚基组成的腈水合酶，使得各个亚基位于不同的质粒上并在大肠杆菌中同时表达。

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