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1. 发明申请

US20100047863A1 GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY 失效
标题翻译：具有广泛基质特征的新型强化微生物氮化物组
公开(公告)号：US20100047863A1
公开(公告)日：2010-02-25
申请号：US12296057
申请日：2007-04-05
申请人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
发明人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
IPC分类号： C12P21/06 , C12P13/02 , C12N15/74 , C12N1/21 , C12N15/63 , C07K14/00 , C07K16/00 , C07H21/04
CPC分类号： C12N9/88 , C12P13/02 , C12P41/006
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites.
摘要翻译：本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化亚硝酸盐

2. 发明授权

US08470576B2 Group of novel enantioselective microbial nitrile hydratases with broad substrate specificity 失效
标题翻译：一组具有广泛底物特异性的新型对映选择性微生物腈水合酶
公开(公告)号：US08470576B2
公开(公告)日：2013-06-25
申请号：US12296057
申请日：2007-04-05
申请人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
发明人： Jürgen Eck , Klaus Liebeton , Lutz Fischer , Sabine Lutz-Wahl , Martina Hensel , Christian Ewert , Marcus Wälz
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/06 , C12P21/04 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/88 , C12P13/02 , C12P41/006
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.
摘要翻译：本发明提供了编码具有腈水合酶（NHase）[E.C.]的酶的多核苷酸或一对多核苷酸。 4.2.1.84]活动。此外，提供了包含所公开的多核苷酸或一对多核苷酸的载体和宿主及其生产方法。此外，本发明涉及一对多肽或具有NHase活性的融合蛋白，特异性结合所述一对多肽或融合蛋白的抗体，在严格条件下与所公开的多核苷酸特异性杂交的引物或探针，或者一对多核苷酸，包含多核苷酸或一对多核苷酸的组合物，一对多肽或融合蛋白，本发明的抗体和/或一种或多种引物或探针以及生产酰胺的方法，其包括对映选择性转化腈类

3. 发明申请

US20090311745A1 GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权
公开(公告)号：US20090311745A1
公开(公告)日：2009-12-17
申请号：US12300275
申请日：2007-05-03
申请人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
发明人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
IPC分类号： C12P21/00 , C12P7/62 , C12P7/40 , C12P7/02 , C12N15/00 , C12N15/74 , C12N9/18 , C12N1/21 , C12N15/63 , C07K16/40 , C07H21/00
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E.C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.

4. 发明授权

US09193961B2 Polynucleotides encoding enantioselective carboxylesterases and methods of making same 有权
标题翻译：编码对映选择性羧酸酯酶的多核苷酸及其制备方法
公开(公告)号：US09193961B2
公开(公告)日：2015-11-24
申请号：US12300275
申请日：2007-05-03
申请人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
发明人： Klaus Liebeton , Jürgen Eck , Uwe Bornscheuer , Dominique Böttcher , Peter Langer , Esen Bellur
IPC分类号： A61K38/00 , C12N9/18
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C.3.1.1.1] activity.
摘要翻译：本发明涉及编码具有羧酸酯酶[E.C.3.1.1.1]活性的酶的多核苷酸。

5. 发明申请

US20110268792A1 SURFACE ACTIVE PROTEINS AS EXCIPIENTS IN SOLID PHARMACEUTICAL FORMULATIONS 有权
标题翻译：表面活性蛋白作为固体药物制剂的优点
公开(公告)号：US20110268792A1
公开(公告)日：2011-11-03
申请号：US13130128
申请日：2009-11-13
申请人： Andreas Buthe , Andreas Hafner , Franz Kaufmann , Esther Gabor , Guido Meurer , Jürgen Eck , Gordon Bradley
发明人： Andreas Buthe , Andreas Hafner , Franz Kaufmann , Esther Gabor , Guido Meurer , Jürgen Eck , Gordon Bradley
IPC分类号： A61K9/48 , A61P39/00 , A61K9/16 , A61K9/02 , A61K9/00 , A61K9/38
CPC分类号： A61K9/4891 , A61K9/0065 , A61K9/2063 , A61K9/2095 , A61K9/2873 , C07K14/37
摘要： The invention relates to a use of surface active hydrophobins for applications in pharmaceutical technology, in particular as excipients for galenic use. Provided is a method for either admixture of hydrophobins to galenic compositions or for treating the surface of pharmaceutical forms with a hydrophobin-containing solution to modify the pharmaceutical properties of the galenic form. In a preferred embodiment of the invention hydrophobins are used to improve the properties of a pharmaceutical composition, e.g. to act as a surfactant or to increase resistance to disintegration of the galenic forms to achieve a retarded drug release. The galenic form to be modified by the use of surface active proteins as excipients can be capsules, tablets, pills, microparticles, vesicles, and suppositories, although further galenic forms are envisioned. The surface active proteins used for the purpose of present invention can either be isolated from their respective natural source or prepared by recombinant techniques and expression in a suitable host.
摘要翻译：本发明涉及用于制药技术中的表面活性疏水蛋白的用途，特别是作为涂覆液使用的赋形剂。提供了将疏水蛋白与盖仑组合物混合或用含疏水蛋白的溶液处理药物形式的表面以改变盖仑膜形式的药物性质的方法。在本发明的优选实施方案中，疏水蛋白用于改善药物组合物的性质，例如，作为表面活性剂或增加耐受霜冻形式的崩解以实现延缓药物释放。通过使用表面活性蛋白质作为赋形剂来修饰的盖仑形式可以是胶囊，片剂，丸剂，微粒，囊泡和栓剂，尽管可以预见到更多的盖仑制剂形式。用于本发明目的的表面活性蛋白质可以从它们各自的天然来源中分离或者通过重组技术制备并在合适的宿主中表达。

6. 发明授权

US06271368B1 Recombinant mistletoe lectin (rML) 失效
标题翻译：重组槲寄生凝集素（rML）
公开(公告)号：US06271368B1
公开(公告)日：2001-08-07
申请号：US08776059
申请日：1997-06-19
申请人： Hans Lentzen , Jürgen Eck , Axel Baur , Holger Zinke
发明人： Hans Lentzen , Jürgen Eck , Axel Baur , Holger Zinke
IPC分类号： C12N121
CPC分类号： C07K14/42 , A61K38/00 , A61K47/6819 , C07K2319/00
摘要： The invention relates to nucleic acid molecules encoding preproproteins having after maturation the biological activity of the mistletoe lectin dimer, to vectors comprising these nucleic acid molecules, to hosts transformed with said vectors and to polypeptides and/or polypeptide dimers which are encoded by these nucleic acid molecules. The polypeptides and/or polypeptide dimers of the invention are widely therapeutically applicable. Thus, the present invention further relates to immunotoxins as well as to pharmaceutical compositions that contain the polypeptides and/or the polypeptide dimers of the invention. Additionally, the invention relates to diagnostic compositions comprising the nucleic acid molecules of the invention, the polypeptides and/or the polypeptide dimers of the invention and/or primers which hybridize specifically to the nucleic acid molecules of the invention. Finally, the invention relates to plant protective agents comprising the polypeptides of the invention and/or the polypeptide dimers of the invention.
摘要翻译：本发明涉及将成熟后槲皮素凝集素二聚体的生物活性的前蛋白质，包含这些核酸分子的载体转染到用所述载体转化的宿主的核酸分子和由这些核酸编码的多肽和/或多肽二聚体分子。本发明的多肽和/或多肽二聚体在广泛的治疗上是适用的。因此，本发明还涉及免疫毒素以及含有本发明的多肽和/或多肽二聚体的药物组合物。另外，本发明涉及包含本发明的核酸分子，本发明的多肽和/或多肽二聚体的诊断组合物和/或与本发明的核酸分子特异性杂交的引物。最后，本发明涉及包含本发明的多肽和/或本发明的多肽二聚体的植物保护剂。

7. 发明授权

US08226967B2 Surface active proteins as excipients in solid pharmaceutical formulations 有权
标题翻译：表面活性蛋白作为固体药物制剂中的赋形剂
公开(公告)号：US08226967B2
公开(公告)日：2012-07-24
申请号：US13130128
申请日：2009-11-13
申请人： Andreas Buthe , Andreas Hafner , Franz Kaufmann , Esther Gabor , Guido Meurer , Jürgen Eck , Gordon Bradley
发明人： Andreas Buthe , Andreas Hafner , Franz Kaufmann , Esther Gabor , Guido Meurer , Jürgen Eck , Gordon Bradley
IPC分类号： A01N25/34 , C07K1/00
CPC分类号： A61K9/4891 , A61K9/0065 , A61K9/2063 , A61K9/2095 , A61K9/2873 , C07K14/37
摘要： The invention relates to a use of surface active hydrophobins for applications in pharmaceutical technology, in particular as excipients for galenic use. Provided is a method for either admixture of hydrophobins to galenic compositions or for treating the surface of pharmaceutical forms with a hydrophobin-containing solution to modify the pharmaceutical properties of the galenic form. In a preferred embodiment of the invention hydrophobins are used to improve the properties of a pharmaceutical composition, e.g. to act as a surfactant or to increase resistance to disintegration of the galenic forms to achieve a retarded drug release. The galenic form to be modified by the use of surface active proteins as excipients can be capsules, tablets, pills, microparticles, vesicles, and suppositories, although further galenic forms are envisioned. The surface active proteins used for the purpose of present invention can either be isolated from their respective natural source or prepared by recombinant techniques and expression in a suitable host.
摘要翻译：本发明涉及用于制药技术中的表面活性疏水蛋白的用途，特别是作为涂覆液使用的赋形剂。提供了将疏水蛋白与盖仑组合物混合或用含疏水蛋白的溶液处理药物形式的表面以改变盖仑膜形式的药物性质的方法。在本发明的优选实施方案中，疏水蛋白用于改善药物组合物的性质，例如，作为表面活性剂或增加耐受霜冻形式的崩解以实现延缓药物释放。通过使用表面活性蛋白质作为赋形剂来修饰的盖仑形式可以是胶囊，片剂，丸剂，微粒，囊泡和栓剂，尽管可以预见到更多的盖仑制剂形式。用于本发明目的的表面活性蛋白质可以从其各自的天然来源分离或通过重组技术制备并在合适的宿主中表达。

8. 发明授权

US07635567B2 Method for determination of the responsiveness of an individual to mistletoe lectin 失效
标题翻译：确定个体对槲寄生凝集素的反应性的方法
公开(公告)号：US07635567B2
公开(公告)日：2009-12-22
申请号：US10499297
申请日：2002-12-20
申请人： Johannes Müthing , Jasna Peter-Katalinic , Martin Langer , Babette Möckel , Jürgen Eck
发明人： Johannes Müthing , Jasna Peter-Katalinic , Martin Langer , Babette Möckel , Jürgen Eck
IPC分类号： G01N33/53 , G01N33/567
CPC分类号： C12Q1/48 , C07K16/18 , G01N33/566 , G01N33/66 , G01N2333/42 , G01N2400/00 , G01N2800/52
摘要： A method for the determination of the responsiveness of an individual to mistletoe lectin or to (an) mistletoe lectin single chain(s), wherein the expression of a membrane-bound receptor for mistletoe lectin is characteristic of a corresponding responsiveness.
摘要翻译：用于测定个体对槲寄生凝集素或（一种）槲寄生凝集素单链的响应性的方法，其中槲寄生凝集素的膜结合受体的表达是相应的响应性的特征。

9. 发明授权

US09353386B2 Means and methods for producing propanediol 有权
标题翻译：制备丙二醇的方法和方法
公开(公告)号：US09353386B2
公开(公告)日：2016-05-31
申请号：US14125409
申请日：2012-06-15
申请人： Daniel Koch , Guido Meurer , Jürgen Eck
发明人： Daniel Koch , Guido Meurer , Jürgen Eck
IPC分类号： C12P7/24 , C12P7/18 , C12N9/04 , C12N9/02 , C12N9/10
CPC分类号： C12P7/24 , C12N9/0006 , C12N9/0008 , C12N9/13 , C12P7/18 , C12Y101/01077 , C12Y102/01 , C12Y208/03001 , Y02P20/52
摘要： The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.
摘要翻译：本发明涉及一种具有升高的酶表达或活性的宿主细胞，与其衍生的亲本细胞相比，所述酶具有乳酰基-CoA还原酶活性。此外，提供了生产乳醛和/或1,2-丙二醇的方法，所述方法包括培养所述宿主细胞和/或利用所述酶产生所述化合物。

10. 发明授权

US08993743B2 Chimeric surface active proteins 有权
标题翻译：嵌合表面活性蛋白
公开(公告)号：US08993743B2
公开(公告)日：2015-03-31
申请号：US13579910
申请日：2011-02-18
申请人： Guido Meurer , Esther Gabor , Anke Bachert , Jürgen Eck
发明人： Guido Meurer , Esther Gabor , Anke Bachert , Jürgen Eck
IPC分类号： C07H21/04 , C07K14/37 , C12N15/62 , A61K9/28 , C09D189/00
CPC分类号： C07K14/37 , A61K9/286 , A61K9/2873 , C09D189/00 , C12N15/62
摘要： The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein. The chimeric protein may only consist of the above mentioned core of (a) and (b), but may also be flanked by additional components of the core, i.e. (a) or (b) or by (an) additional complete core(s) (a) and (b). The present invention furthermore relates to a method of coating and/or impregnating a material, comprising contacting the material with the chimeric protein or the composition of the invention.
摘要翻译：本发明涉及编码具有表面活性蛋白的生化活性的嵌合蛋白的核酸分子，其中所述嵌合蛋白包含：（a）第一表面活性蛋白的N末端部分，其中所述N末端部分没有成熟的第一表面活性蛋白的最多N末端氨基酸的0和10之间; 并且其C末端，（b）第二表面活性蛋白质的C末端部分，其中所述C末端部分缺少所述成熟第二表面活性蛋白质的最C末端氨基酸的0至10个。本发明还涉及载体，非人宿主和生产具有表面活性蛋白的生化活性的嵌合蛋白的方法。此外，本发明涉及由本发明的核酸分子编码的嵌合蛋白质和包含该嵌合蛋白质的组合物。嵌合蛋白质只能由上述（a）和（b）的核心组成，但也可以由核心的另外的组分，即（a）或（b）或另外一个完整的核心）（a）和（b）。本发明还涉及一种涂覆和/或浸渍材料的方法，包括使材料与本发明的嵌合蛋白或组合物接触。

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