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1. 发明授权

US07491521B2 Rhodococcus nitrile hydratase 有权
标题翻译：红球菌腈水合酶
公开(公告)号：US07491521B2
公开(公告)日：2009-02-17
申请号：US11892085
申请日：2007-08-20
申请人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
发明人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
IPC分类号： C12P13/02 , C12P21/06 , C12N9/08 , C07H21/04
CPC分类号： C07K14/47 , C12N9/88 , C12P13/02
摘要： The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
摘要翻译：本发明涉及一种红球菌多核苷酸簇，其含有编码具有腈水合酶活性的多肽，辅酶P15K，其活化该酶和钴转运蛋白的核苷酸序列，转化微生物，其中编码这些蛋白质的核苷酸序列为存在增加的量，以及使用转化的微生物从腈制备酰胺。

2. 发明授权

US07288402B2 Rhodococcus nitrile hydratase 有权
标题翻译：红球菌腈水合酶
公开(公告)号：US07288402B2
公开(公告)日：2007-10-30
申请号：US11083327
申请日：2005-03-18
申请人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
发明人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
IPC分类号： C12N9/80 , C12N9/08 , C07H21/04 , C12P21/06 , C12P13/02
CPC分类号： C07K14/47 , C12N9/88 , C12P13/02
摘要： The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
摘要翻译：本发明涉及一种红球菌多核苷酸簇，其含有编码具有腈水合酶活性的多肽，辅酶P15K，其活化该酶和钴转运蛋白的核苷酸序列，转化微生物，其中编码这些蛋白质的核苷酸序列为存在增加的量，以及使用转化的微生物从腈制备酰胺。

3. 发明申请

US20060068467A1 Rhodococcus nitrile hydratase 有权
公开(公告)号：US20060068467A1
公开(公告)日：2006-03-30
申请号：US11083327
申请日：2005-03-18
申请人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
发明人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konrad Odendahl
IPC分类号： C12P13/02 , C07H21/04 , C12P21/06 , C12N9/80 , C12N1/20 , C12N15/74
CPC分类号： C07K14/47 , C12N9/88 , C12P13/02
摘要： The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

4. 发明申请

US20100021977A1 PROCESS FOR PREPARING ALKYL (METH)ACRYLATES USING AN ENZYMATIC CYANOHYDRIN HYDROLYSIS 审中-公开
标题翻译：制备丙烯酸（甲基）丙烯酸酯的方法，使用酶促氰化氢水解
公开(公告)号：US20100021977A1
公开(公告)日：2010-01-28
申请号：US12441145
申请日：2007-08-30
申请人： Alexander May , Jochen Ackermann , Hermann Siegert , Bernd Vogel , Steffen Osswald
发明人： Alexander May , Jochen Ackermann , Hermann Siegert , Bernd Vogel , Steffen Osswald
IPC分类号： C12P7/62
CPC分类号： C12P7/62 , C12P13/02
摘要： The present invention relates to a process for preparing alkyl(meth)acrylates, characterized in that the process has a step in which a cyanohydrin is hydrolysed with an enzyme whose residual activity after the conversion of methacrylonitrile in the presence of 20 mM cyanide ions at 20° C. after 30 min is at least 90% of the residual activity of the enzyme which has been used under otherwise identical conditions in the absence of cyanide ions.
摘要翻译：本发明涉及一种制备（甲基）丙烯酸烷基酯的方法，其特征在于该方法具有以下步骤：将氰醇与20mM氰化物离子存在下甲基丙烯腈转化后的残留活性为20的酶水解 30分钟后，在不存在氰化物离子的条件下使用的酶的残留活性至少为90％。

5. 发明申请

US20080057549A1 Rhodococcus nitrile hydratase 有权
标题翻译：红球菌腈水合酶
公开(公告)号：US20080057549A1
公开(公告)日：2008-03-06
申请号：US11892085
申请日：2007-08-20
申请人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konard Odendahl
发明人： Steffen Osswald , Stefan Verseck , Uta Deiting , Christoph Weckbecker , Klaus Huthmacher , Michael Binder , Maria-Regina Kula , Konard Odendahl
IPC分类号： C12P13/02
CPC分类号： C07K14/47 , C12N9/88 , C12P13/02
摘要： The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
摘要翻译：本发明涉及一种红球菌多核苷酸簇，其含有编码具有腈水合酶活性的多肽，辅酶P15K，其活化该酶和钴转运蛋白的核苷酸序列，转化微生物，其中编码这些蛋白质的核苷酸序列为存在增加的量，以及使用转化的微生物从腈制备酰胺。

6. 发明申请

US20080248538A1 Cyanide-Tolerant Nitrile Hydratases 有权
标题翻译：氰化物耐受腈水合酶
公开(公告)号：US20080248538A1
公开(公告)日：2008-10-09
申请号：US10598873
申请日：2005-03-14
申请人： Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher , Tatijana Gerasimova , Andrey Novikov , Ludmila Ryabchenko , Alexander Yanenko , Ksenia Egorova
发明人： Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher , Tatijana Gerasimova , Andrey Novikov , Ludmila Ryabchenko , Alexander Yanenko , Ksenia Egorova
IPC分类号： C12P13/02 , C07H21/04 , C07K16/00 , C12N15/00 , C12N1/20 , C12N9/00
CPC分类号： C12N9/88 , C12P13/02
摘要： Disclosed herein are cyanide-tolerant nitrile hydratases especially from Pseudomonas putida or Pseudomonas marginalis strains which exhibit increased cyanide tolerance. Also disclosed are methods of preparing amides from nitriles in the presence of cyanides and polynucleotide sequences coding for cyanide-tolerant nitrile hydratases.
摘要翻译：本文公开的是氰化物耐受性腈水合酶，特别是恶臭假单胞菌（Pseudomonas putida）或边缘假单胞菌（Pseudomonas marginalis）的菌株，其表现出增加的氰化物耐受性。还公开了在氰化物和编码氰化物耐受性腈水合酶的多核苷酸序列存在下由腈制备酰胺的方法。

7. 发明授权

US07217544B2 Method for the preparation of L-amino acids from D-amino acids 有权
标题翻译：从D-氨基酸制备L-氨基酸的方法
公开(公告)号：US07217544B2
公开(公告)日：2007-05-15
申请号：US11056165
申请日：2005-02-14
申请人： Werner Hummel , Birgit Geueke , Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher
发明人： Werner Hummel , Birgit Geueke , Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher
IPC分类号： C12P13/04 , C12P21/06 , C12N9/06 , C12N1/20 , C12N9/02 , C07H21/04
CPC分类号： C12P13/04 , C12N9/0016 , C12N9/0024 , C12N9/0065 , C12P13/06 , C12P13/12
摘要： The invention relates to recombinant microorganisms which, in comparison to the starting organism, have a higher concentration or activity of a D-amino acid oxidase, of an L-amino acid dehydrogenase, of an NADH cosubstrate regenerating enzyme and, if necessary, of a catalase. The invention also includes methods for preparing L-amino acids from D-amino acids using of these microorganisms.
摘要翻译：本发明涉及重组微生物，其与起始生物体相比，具有较高浓度或活性的L-氨基酸脱氢酶，NADH辅助底物再生酶，以及必要时具有过氧化氢酶。本发明还包括使用这些微生物从D-氨基酸制备L-氨基酸的方法。

8. 发明申请

US20060063238A1 Method for the preparation of L-amino acids from D-amino acids 有权
标题翻译：从D-氨基酸制备L-氨基酸的方法
公开(公告)号：US20060063238A1
公开(公告)日：2006-03-23
申请号：US11056165
申请日：2005-02-14
申请人： Werner Hummel , Birgit Geueke , Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher
发明人： Werner Hummel , Birgit Geueke , Steffen Osswald , Christoph Weckbecker , Klaus Huthmacher
IPC分类号： C12P13/04 , C12N9/06 , C12N1/21
CPC分类号： C12P13/04 , C12N9/0016 , C12N9/0024 , C12N9/0065 , C12P13/06 , C12P13/12
摘要： The invention relates to recombinant microorganisms which, in comparison to the starting organism, have a higher concentration or activity of a D-amino acid oxidase, of an L-amino acid dehydrogenase, of an NADH cosubstrate regenerating enzyme and, if necessary, of a catalase. The invention also includes methods for preparing L-amino acids from D-amino acids using of these microorganisms.
摘要翻译：本发明涉及重组微生物，其与起始生物体相比，具有较高浓度或活性的L-氨基酸脱氢酶，NADH辅助底物再生酶，以及必要时具有过氧化氢酶。本发明还包括使用这些微生物从D-氨基酸制备L-氨基酸的方法。

9. 发明授权

US09358648B2 Processing apparatus and method of operating a processing apparatus 有权
标题翻译：处理装置和操作处理装置的方法
公开(公告)号：US09358648B2
公开(公告)日：2016-06-07
申请号：US13610026
申请日：2012-09-11
申请人： Joachim Weber , Steffen Osswald , Stefan Drexler , Joachim Schulz
发明人： Joachim Weber , Steffen Osswald , Stefan Drexler , Joachim Schulz
IPC分类号： B23P21/00 , B23Q39/04 , B21D43/02 , B21D43/14 , B21D51/26
CPC分类号： B23P21/006 , B21D43/028 , B21D43/14 , B21D51/2692 , B23Q39/046 , Y10T29/49998 , Y10T29/5127 , Y10T29/5129 , Y10T29/519
摘要： A processing apparatus for container blanks, with a machine bed, a feed device for feeding container blanks, a discharge device for removal of container blanks, a rotatably mounted rotary worktable with several locating devices designed for conveyance of container blanks along a circular orbit section shaped conveyance path, wherein the conveyance path determines a first circle segment shaped surface section, several operating devices lying opposite the rotary worktable in the radial direction, and at least one conveyor designed for conveyance of components of container blanks and/or for container blanks along a conveyor section between a component mounting and an assigned operating device. The component mounting of the conveyor is located in a second circle segment shaped surface section, which supplements the first surface section determined by the circular orbit section shaped conveyance path to make a full circle.
摘要翻译：一种用于容器坯料的加工设备，其具有机床，用于供应容器坯料的进料装置，用于移除容器坯料的排出装置，具有多个定位装置的可旋转地安装的旋转工作台，所述定位装置设计成沿着圆形轨道部分形状的运输容器坯料输送路径，其中所述输送路径确定第一圆形段形状表面区段，在径向方向上与所述旋转工作台相对的若干操作装置，以及至少一个设计用于沿着沿着所述径向移动的容器坯料和/或容器坯料的部件的输送的输送器部件安装和分配的操作装置之间的输送机部分。输送机的部件安装位于第二圆形段形表面部分中，其补充由圆形轨道部分形状输送路径确定的第一表面部分以形成整圆。

10. 发明申请

US20080102496A1 Expression of Nitrile Hydratases in a Two-Vector Expression System 审中-公开
标题翻译：腈水合酶在双向表达系统中的表达
公开(公告)号：US20080102496A1
公开(公告)日：2008-05-01
申请号：US10593362
申请日：2005-02-18
申请人： Stefan Verseck , Steffen Osswald , Wai-Yee Phong , Klaus Liebeton , Jurgen Eck
发明人： Stefan Verseck , Steffen Osswald , Wai-Yee Phong , Klaus Liebeton , Jurgen Eck
IPC分类号： C12N1/20 , C12N15/00 , C12N15/64 , C12N9/88 , C12P7/40
CPC分类号： C12N15/70 , C12N9/88
摘要： The present invention relates to a novel system for expressing nitrile hydratase. In this system, the nitrile hydratases, which are composed of subunits, are formed such that the respective subunits are located on different plasmids and are expressed simultaneously in E. coli.
摘要翻译：本发明涉及一种表达腈水合酶的新型体系。在该系统中，形成由亚基组成的腈水合酶，使得各个亚基位于不同的质粒上并在大肠杆菌中同时表达。

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