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1. 发明申请

US20130029396A1 Multifunctional Biomemory Device 有权
标题翻译：多功能生物记录仪
公开(公告)号：US20130029396A1
公开(公告)日：2013-01-31
申请号：US13404696
申请日：2012-02-24
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
IPC分类号： C12N11/16 , C07K14/825 , C07K14/00
CPC分类号： C07K14/00 , C12N9/0004 , C12N11/00 , C12N11/14 , C12Y120/09001
摘要： The present disclosure relates to a multifunctional biomemory device in which a protein having a redox potential substituted with a metal ion is directly immobilized on a substrate. The present disclosure provides an operating method in which the redox state of the protein is controlled by applying three different potentials. The present disclosure provides a biomemory device in which the metal ion of a metalloprotein is substituted to allow for artificial control of the redox potential. The present disclosure provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.
摘要翻译：本公开内容涉及一种多功能生物记录装置，其中具有被金属离子取代的氧化还原电位的蛋白质直接固定在基底上。本公开提供了通过应用三种不同电位来控制蛋白质的氧化还原状态的操作方法。本公开提供了其中金属蛋白的金属离子被替代以允许人为控制氧化还原电位的生物记忆装置。本公开提供了一种基于天然存在的生物分子的电子转移原理的作为信息存储装置的新概念生物记忆装置。

2. 发明申请

US20100270543A1 Biomolecule-Based Electronic Device 有权
标题翻译：基于生物分子的电子设备
公开(公告)号：US20100270543A1
公开(公告)日：2010-10-28
申请号：US12809646
申请日：2008-04-17
申请人： Jeong-Woo Choi , Jun-Hong Min , Byung-Keun Oh , Hyun-Hee Kim , Young-Jun Kim
发明人： Jeong-Woo Choi , Jun-Hong Min , Byung-Keun Oh , Hyun-Hee Kim , Young-Jun Kim
IPC分类号： H01L51/30
CPC分类号： G11C13/0014 , B82Y10/00 , G11C13/0009 , G11C13/0019 , H01L51/0093 , H01L51/0591
摘要： The present invention relates to a biomolecule-based electronic device in which the biomolecule with redox potential is directly immobilized on the substrate. The present invention enables to excellently exhibit the capability of a protein-based bio-memory device in which it is preferable to use the substrate on which cysteine-introduced recombinant proteins are effectively immobilized and a self-assembled layer (SAM) is fabricated. It becomes realized that a redox potential is regulated using intrinsic redox potential of the protein dependent on applied voltage. The present invention provides a novel operating method in which three potentials are applied throughout four steps. The present invention has some advantages of fabricating a protein layer in a convenient manner and inducing electron transfer by fundamental electrochemical or electronic operation. The method of this invention is considered as a new concept in the senses that intrinsic electron transfer mechanisms induced by natural-occurring biomolecules are used to develop an information storage device.
摘要翻译：本发明涉及一种基于生物分子的电子器件，其中具有氧化还原电位的生物分子直接固定在基底上。本发明能够优异地表现出蛋白质生物记忆装置的能力，其中优选使用其上有效固定半胱氨酸导入的重组蛋白质的底物和制造自组装层（SAM）。实现使用依赖于施加电压的蛋白质的固有氧化还原电位来调节氧化还原电位。本发明提供一种新的操作方法，其中三个电位通过四个步骤施加。本发明具有以方便的方式制造蛋白质层并通过基本电化学或电子操作诱导电子转移的一些优点。本发明的方法被认为是用天然存在的生物分子诱导的固有电子传递机制来开发信息存储装置的感觉的新概念。

3. 发明授权

US07510865B2 Sensing switch and detecting method using the same 失效
标题翻译：感应开关及使用其的检测方法
公开(公告)号：US07510865B2
公开(公告)日：2009-03-31
申请号：US11347185
申请日：2006-02-03
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
IPC分类号： C12M1/34
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

4. 发明申请

US20070238114A1 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US20070238114A1
公开(公告)日：2007-10-11
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

5. 发明申请

US20060183112A1 Method of separating biomolecules using nanopore 审中-公开
标题翻译：使用纳米孔分离生物分子的方法
公开(公告)号：US20060183112A1
公开(公告)日：2006-08-17
申请号：US11335246
申请日：2006-01-19
申请人： Jun-hong Min , Su-hyeon Kim , In-ho Lee , Kui-hyun Kim , Seung-yeon Yang
发明人： Jun-hong Min , Su-hyeon Kim , In-ho Lee , Kui-hyun Kim , Seung-yeon Yang
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： G01N33/5438 , G01N33/48721
摘要： Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the target biomolecule is not bound in the first chamber; applying a voltage which has the same polarity as that of the target biomolecule to the electrode of the first chamber, and a voltage which has an opposite charge to that of the target biomolecule to the electrode of the second chamber; and translocating only the particles to which the target biomolecule is bound from the first chamber to the second chamber through the pore. Conventionally, the size of a pore is used to separate biomolecules. However, effective separation is difficult to achieve because the manufacture of a pore with a diameter of less than 10 nm, small enough to separate biomolecule, is not easy. Therefore, signal separation and data analysis must be required. However, in the present method, physical movement induced by the charge of biomolecules is used to effectively separate the biomolecules, thus obtaining a high signal to noise ratio. As a result, additional data analysis is not required.
摘要翻译：提供了一种分离颗粒的方法，该方法包括：形成由与孔的界面分隔开的第一室和第二室，其中第一和第二室具有不同极性的电极; 在第一室中放置靶生物分子所结合的颗粒与目标生物分子不结合的颗粒; 将具有与目标生物分子相同极性的电压施加到第一室的电极，以及将具有与靶生物分子相反的电荷的电压施加到第二室的电极; 并且仅将来自第一室的目标生物分子结合的颗粒通过孔转移到第二室。通常，使用孔的大小来分离生物分子。然而，由于直径小于10nm的孔的制造足够小以分离生物分子，所以难以实现有效的分离，这是不容易的。因此，必须要求信号分离和数据分析。然而，在本方法中，由生物分子的电荷引起的身体运动用于有效分离生物分子，从而获得高的信噪比。因此，不需要额外的数据分析。

6. 发明申请

US20060134777A1 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US20060134777A1
公开(公告)日：2006-06-22
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： G01N27/447 , C12M1/33
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

7. 发明授权

US09796789B2 Biomemory device comprising heterolayer of recombinant protein and inorganic particle 有权
公开(公告)号：US09796789B2
公开(公告)日：2017-10-24
申请号：US13564136
申请日：2012-08-01
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
IPC分类号： C07K17/14 , C12N11/14 , G11C13/00 , B82Y10/00 , B82Y5/00 , B82Y30/00 , B82Y15/00
CPC分类号： C07K17/14 , B82Y10/00 , B82Y15/00 , B82Y30/00 , C12N11/14 , G11C13/0019
摘要： The present invention relates to a biomemory device, comprising (a) a substrate; and (b) a heterolayer comprising a protein having a redox potential and an inorganic particle; wherein the heterolayer is immobilized on the substrate. By applying inorganic particles, the present invention provides a biomemory device capable of enhancing low current signals detected electron transfer between biomolecules and substrates up to at least five (5) times greater signals. The present invention is capable of controlling the redox states with help of redox potentials of proteins depending on applied potential. The present invention provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.

8. 发明授权

US08658359B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US08658359B2
公开(公告)日：2014-02-25
申请号：US13079926
申请日：2011-04-05
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

9. 发明申请

US20110177588A1 METHOD AND APPARATUS FOR DISRUPTING CELLS AND PURIFYING NUCLEIC ACID USING A SINGLE CHIP 审中-公开
公开(公告)号：US20110177588A1
公开(公告)日：2011-07-21
申请号：US13079910
申请日：2011-04-05
申请人： In-ho LEE , Jun-hong MIN , Young-rok KIM , Jae-ho YOU , Chang-eun YOO , Jeong-gun LEE , Ki-woong HAN
发明人： In-ho LEE , Jun-hong MIN , Young-rok KIM , Jae-ho YOU , Chang-eun YOO , Jeong-gun LEE , Ki-woong HAN
IPC分类号： C12M1/42 , C12M1/00
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

10. 发明授权

US07955801B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US07955801B2
公开(公告)日：2011-06-07
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

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