专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060183112A1 Method of separating biomolecules using nanopore 审中-公开
标题翻译：使用纳米孔分离生物分子的方法
公开(公告)号：US20060183112A1
公开(公告)日：2006-08-17
申请号：US11335246
申请日：2006-01-19
申请人： Jun-hong Min , Su-hyeon Kim , In-ho Lee , Kui-hyun Kim , Seung-yeon Yang
发明人： Jun-hong Min , Su-hyeon Kim , In-ho Lee , Kui-hyun Kim , Seung-yeon Yang
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： G01N33/5438 , G01N33/48721
摘要： Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the target biomolecule is not bound in the first chamber; applying a voltage which has the same polarity as that of the target biomolecule to the electrode of the first chamber, and a voltage which has an opposite charge to that of the target biomolecule to the electrode of the second chamber; and translocating only the particles to which the target biomolecule is bound from the first chamber to the second chamber through the pore. Conventionally, the size of a pore is used to separate biomolecules. However, effective separation is difficult to achieve because the manufacture of a pore with a diameter of less than 10 nm, small enough to separate biomolecule, is not easy. Therefore, signal separation and data analysis must be required. However, in the present method, physical movement induced by the charge of biomolecules is used to effectively separate the biomolecules, thus obtaining a high signal to noise ratio. As a result, additional data analysis is not required.
摘要翻译：提供了一种分离颗粒的方法，该方法包括：形成由与孔的界面分隔开的第一室和第二室，其中第一和第二室具有不同极性的电极; 在第一室中放置靶生物分子所结合的颗粒与目标生物分子不结合的颗粒; 将具有与目标生物分子相同极性的电压施加到第一室的电极，以及将具有与靶生物分子相反的电荷的电压施加到第二室的电极; 并且仅将来自第一室的目标生物分子结合的颗粒通过孔转移到第二室。通常，使用孔的大小来分离生物分子。然而，由于直径小于10nm的孔的制造足够小以分离生物分子，所以难以实现有效的分离，这是不容易的。因此，必须要求信号分离和数据分析。然而，在本方法中，由生物分子的电荷引起的身体运动用于有效分离生物分子，从而获得高的信噪比。因此，不需要额外的数据分析。

2. 发明申请

US20060188907A1 Patterning method, substrate for biomolecule immobilization using the patterning method, and biochip employing the substrate 审中-公开
标题翻译：图案化方法，使用图案化方法的生物分子固定用基材和使用该基材的生物芯片
公开(公告)号：US20060188907A1
公开(公告)日：2006-08-24
申请号：US11336294
申请日：2006-01-18
申请人： In-ho Lee , Jun-hong Min , Su-hyeon Kim , Kui-hyun Kim , Seung-yeon Yang
发明人： In-ho Lee , Jun-hong Min , Su-hyeon Kim , Kui-hyun Kim , Seung-yeon Yang
IPC分类号： C12Q1/68 , C12M1/34 , H01L21/00
CPC分类号： C23C18/1893 , B01J19/0046 , B01J2219/00527 , B01J2219/00596 , B01J2219/00603 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , C23C18/1608 , C23C18/2086 , C23C18/44 , C40B40/06 , C40B40/10 , G01N33/54353 , H05K3/389 , H05K2203/013
摘要： Provided is a linker functional group patterning method for biomolecule immobilization. The patterning method includes preparing a coating composition including a hydrophobic group-containing silane compound and a hydrophilic group-containing silane compound; forming a surface tension control layer by coating the coating composition on a substrate for biomoleucle immobilization; and forming a linker functional group pattern on the surface tension control layer using a coating composition including a linker functional group-containing compound followed by thermal treatment. The linker functional group pattern is formed in a uniform size and distribution and contains high-density linker functional groups.
摘要翻译：提供了用于生物分子固定的接头官能团图案化方法。图案化方法包括制备包含含疏水基的硅烷化合物和含亲水基团的硅烷化合物的涂料组合物; 通过将所述涂料组合物涂布在用于生物核固定的底物上来形成表面张力控制层; 以及使用包含连接官能团的化合物的涂层组合物在表面张力控制层上形成接头官能团图案，随后进行热处理。连接体官能团图案形成均匀的大小和分布，并含有高密度接头官能团。

3. 发明申请

US20060118417A1 Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
标题翻译：使用氢键和电场的核酸纯化方法
公开(公告)号：US20060118417A1
公开(公告)日：2006-06-08
申请号：US11281226
申请日：2005-11-16
申请人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
发明人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
IPC分类号： C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.
摘要翻译：提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

4. 发明申请

US20090169431A1 METHOD FOR HIGHLY SENSITIVE NUCLEIC ACID DETECTION USING NANOPORE AND NON-SPECIFIC NUCLEIC ACID BINDING AGENT 审中-公开
标题翻译：使用纳米和非特异性核酸结合剂进行敏感性核酸检测的方法
公开(公告)号：US20090169431A1
公开(公告)日：2009-07-02
申请号：US12364235
申请日：2009-02-02
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
IPC分类号： G01N27/00
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.
摘要翻译：提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

5. 发明授权

US07504261B2 Method for highly sensitive nucleic acid detection using nanopore and non-specific nucleic acid-binding agent 失效
标题翻译：使用纳米孔和非特异性核酸结合剂的高灵敏度核酸检测方法
公开(公告)号：US07504261B2
公开(公告)日：2009-03-17
申请号：US11414022
申请日：2006-04-28
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
IPC分类号： G01N33/00 , G01N33/53 , C07H21/04
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.
摘要翻译：提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

6. 发明申请

US20060292605A1 Method for highly sensitive nucleic acid detection using nanopore and non-specific nucleic acid-binding agent 失效
公开(公告)号：US20060292605A1
公开(公告)日：2006-12-28
申请号：US11414022
申请日：2006-04-28
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.

7. 发明申请

US20060199199A1 Method of removing nucleic acid amplification inhibitor from biological sample and PCR system 审中-公开
标题翻译：从生物样品和PCR系统中去除核酸扩增抑制剂的方法
公开(公告)号：US20060199199A1
公开(公告)日：2006-09-07
申请号：US11336568
申请日：2006-01-20
申请人： Kui-hyun Kim , Young-a Kim , Jun-hong Min , Jeong-gun Lee , In-ho Lee
发明人： Kui-hyun Kim , Young-a Kim , Jun-hong Min , Jeong-gun Lee , In-ho Lee
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34
CPC分类号： C12Q1/6848 , B01L3/5027 , C12Q2563/149 , C12Q2527/125
摘要： Provided is a method of removing a nucleic acid amplification inhibitor from a biological sample. The method includes contacting the biological sample to a carboxyl group-coated solid support. Provided is also a micro-PCR system including a sample pretreatment chamber including a carboxyl group-coated solid support; a PCR chamber; and a channel connecting the sample pretreatment chamber and the PCR chamber.
摘要翻译：提供从生物样品中除去核酸扩增抑制剂的方法。该方法包括使生物样品与羧基涂覆的固体支持物接触。还提供了一种微型PCR系统，其包括含有羧基的固体支持物的样品预处理室; PCR室; 以及连接样品预处理室和PCR室的通道。

8. 发明授权

US07572644B2 Hydrogel copolymer, substrate coated with the copolymer, method of producing microarray using the copolymer, and microarray produced by the method 有权
标题翻译：水凝胶共聚物，用共聚物涂布的基材，使用共聚物制备微阵列的方法，以及通过该方法制备的微阵列
公开(公告)号：US07572644B2
公开(公告)日：2009-08-11
申请号：US11334145
申请日：2006-01-17
申请人： In-ho Lee , Jun-hong Min , Su-hyeon Kim
发明人： In-ho Lee , Jun-hong Min , Su-hyeon Kim
IPC分类号： G01N33/547 , G01N33/544 , G01N33/53 , C12M1/34 , C07K1/14 , C07D207/00
CPC分类号： C12N11/06 , B82Y30/00 , B82Y40/00 , C08F220/28 , C08F220/56 , C08F220/60 , C08F230/08 , C12Q1/6837 , G01N2035/00158 , Y10S524/916
摘要： A novel hydrogel copolymer, a substrate coated with the copolymer, a method of producing a microarray using the copolymer, and a microarray produced by the method are provided. The use of the hydrogel copolymer makes efficient removal of protein and high integration of nucleic acid and protein on a substrate for a microarray possible.
摘要翻译：提供一种新型水凝胶共聚物，用共聚物涂布的基材，使用该共聚物制备微阵列的方法，以及通过该方法制备的微阵列。使用水凝胶共聚物可有效去除蛋白质，核基酸和蛋白质在基质上的高度整合可用于微阵列。

9. 发明申请

US20060110760A1 Microarray using laminar flow and method of preparing the same 审中-公开
标题翻译：使用层流的微阵列及其制备方法
公开(公告)号：US20060110760A1
公开(公告)日：2006-05-25
申请号：US11269037
申请日：2005-11-07
申请人： Su-hyeon Kim , In-ho Lee , Jun-hong Min
发明人： Su-hyeon Kim , In-ho Lee , Jun-hong Min
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00369 , B01J2219/00432 , B01J2219/005 , B01J2219/0052 , B01J2219/00641 , B01J2219/00644 , B01J2219/00657 , B01J2219/00673 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00743
摘要： A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.
摘要翻译：提供了包含水凝胶和固定在水凝胶的离散区域中的多个探针的微阵列及其制备方法。当使用微阵列和方法时，不需要固体底物，并且许多生物分子可以以小体积固定，从而获得高灵敏度。由于可以切割凝胶以获得许多片，因此可以一次制备许多微阵列。

10. 发明申请

US20060160120A1 Novel hydrogel copolymer, substrate coated with the copolymer, method of producing microarray using the copolymer, and microarray produced by the method 有权
标题翻译：新型水凝胶共聚物，用共聚物涂布的基材，使用该共聚物制造微阵列的方法，以及通过该方法制备的微阵列
公开(公告)号：US20060160120A1
公开(公告)日：2006-07-20
申请号：US11334145
申请日：2006-01-17
申请人： In-ho Lee , Jun-hong Min , Su-hyeon Kim
发明人： In-ho Lee , Jun-hong Min , Su-hyeon Kim
IPC分类号： G01N1/28 , C12Q1/68 , G01N33/53 , C12M1/34 , B05D3/02
CPC分类号： C12N11/06 , B82Y30/00 , B82Y40/00 , C08F220/28 , C08F220/56 , C08F220/60 , C08F230/08 , C12Q1/6837 , G01N2035/00158 , Y10S524/916
摘要： A novel hydrogel copolymer, a substrate coated with the copolymer, a method of producing a microarray using the copolymer, and a microarray produced by the method are provided. The use of the hydrogel copolymer makes efficient removal of protein and high integration of nucleic acid and protein on a substrate for a microarray possible.
摘要翻译：提供一种新型水凝胶共聚物，用共聚物涂布的基材，使用该共聚物制备微阵列的方法，以及通过该方法制备的微阵列。使用水凝胶共聚物可有效去除蛋白质，核基酸和蛋白质在基质上的高度整合可用于微阵列。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式