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1. 发明授权

US09024111B1 Methods and materials for deconstruction of biomass for biofuels production 有权
标题翻译：用于生物燃料生产的解构方法和材料
公开(公告)号：US09024111B1
公开(公告)日：2015-05-05
申请号：US13436508
申请日：2012-03-30
申请人： Joseph S. Schoeniger , Masood Zia Hadi
发明人： Joseph S. Schoeniger , Masood Zia Hadi
IPC分类号： C12N15/82 , C12N15/63 , C12N15/09 , A01H5/04 , C12N9/42
CPC分类号： C12N9/2437 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8255
摘要： The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.
摘要翻译：本发明涉及用于生产生物燃料的核酸，肽，载体，细胞和植物。在某些实施方案中，本发明涉及可用于水解植物细胞壁材料的来自极端生物体的核酸序列和肽，例如SSO1949和Ce1A。在另外的实施方案中，本发明涉及已经优化用于在单子叶植物和双子叶植物中的一个或两个中生产的这种序列的修饰形式。在其它实施方案中，本发明提供将肽生产或活性靶向细胞或生物体内的某些位置，例如质外体。在另外的实施方案中，本发明涉及转化的细胞或植物。在另外的实施方案中，本发明涉及利用这种核酸，肽，靶向序列，载体，细胞和/或植物生产生物燃料的方法。

2. 发明授权

US06428666B1 Electrokinetic concentration of charged molecules 有权
标题翻译：带电分子的电动浓度
公开(公告)号：US06428666B1
公开(公告)日：2002-08-06
申请号：US09256586
申请日：1999-02-22
申请人： Anup K. Singh , David W. Neyer , Joseph S. Schoeniger , Michael G. Garguilo
发明人： Anup K. Singh , David W. Neyer , Joseph S. Schoeniger , Michael G. Garguilo
IPC分类号： G01N2726
CPC分类号： G01N30/00 , B01J4/04 , B01J19/2475 , G01N1/40 , G01N27/44743 , G01N2030/0035 , G01N2030/285
摘要： A method for separating and concentrating charged species from uncharged or neutral species regardless of size differential. The method uses reversible electric field induced retention of charged species, that can include molecules and molecular aggregates such as dimers, polymers, multimers, colloids, micelles, and liposomes, in volumes and on surfaces of porous materials. The retained charged species are subsequently quantitatively removed from the porous material by a pressure driven flow that passes through the retention volume and is independent of direction thus, a multi-directional flow field is not required. Uncharged species pass through the system unimpeded thus effecting a complete separation of charged and uncharged species and making possible concentration factors greater than 1000-fold.
摘要翻译：一种用于从不带电荷或中性物质分离和浓缩带电物质的方法，而不考虑大小差异。该方法在多孔材料的体积和表面上使用可逆电场诱导的带电物质的保留，其可以包括分子和分子聚集体，例如二聚体，聚合物，多聚体，胶体，胶束和脂质体。随后通过通过保持体积的压力驱动流从多孔材料中定量地除去保留的带电物质，并且不依赖于方向，因此不需要多方向流场。不带电荷的物质无阻碍地通过系统，从而完全分离带电荷和不带电荷的物质，并使得可能的浓度因子大于1000倍。

3. 发明授权

US06210986B1 Microfluidic channel fabrication method 有权
标题翻译：微流体通道制造方法
公开(公告)号：US06210986B1
公开(公告)日：2001-04-03
申请号：US09404945
申请日：1999-09-23
申请人： Don W. Arnold , Joseph S. Schoeniger , Gregory F. Cardinale
发明人： Don W. Arnold , Joseph S. Schoeniger , Gregory F. Cardinale
IPC分类号： G01N2726
CPC分类号： G01N27/44791 , B01L3/502707 , B01L3/50273 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2400/0415 , B01L2400/0487 , B81C1/00071 , B81C2201/019
摘要： A new channel structure for microfluidic systems and process for fabricating this structure. In contrast to the conventional practice of fabricating fluid channels as trenches or grooves in a substrate, fluid channels are fabricated as thin walled raised structures on a substrate. Microfluidic devices produced in accordance with the invention are a hybrid assembly generally consisting of three layers: 1) a substrate that can or cannot be an electrical insulator; 2) a middle layer, that is an electrically conducting material and preferably silicon, forms the channel walls whose height defines the channel height, joined to and extending from the substrate; and 3) a top layer, joined to the top of the channels, that forms a cover for the channels. The channels can be defined by photolithographic techniques and are produced by etching away the material around the channel walls.
摘要翻译：用于微流体系统的新通道结构和用于制造该结构的工艺。与在衬底中制造流体通道作为沟槽或凹槽的常规实践相反，流体通道在衬底上制造为薄壁凸起结构。根据本发明制造的微流体装置是通常由三层组成的混合组件：1）可以是或不能是电绝缘体的衬底; 2）中间层，其是导电材料，优选硅，形成通道壁，其高度限定了连接到衬底并从衬底延伸的通道高度; 和3）连接到通道的顶部的顶层，其形成用于通道的盖。通道可以通过光刻技术来定义，并且通过蚀刻掉通道壁周围的材料来产生。

4. 发明授权

US06960285B2 Electrokinetically pumped high pressure sprays 有权
标题翻译：电动泵送高压喷雾
公开(公告)号：US06960285B2
公开(公告)日：2005-11-01
申请号：US10260164
申请日：2002-09-30
申请人： Joseph S. Schoeniger , Phillip H. Paul , Luke Schoeniger
发明人： Joseph S. Schoeniger , Phillip H. Paul , Luke Schoeniger
IPC分类号： A61M37/00 , B05B5/00 , B05B5/025 , B05B5/16 , F04B17/00 , F04B19/00 , A61F13/00
CPC分类号： F04B17/00 , A61M37/00 , B05B5/00 , B05B5/025 , B05B5/1691 , F04B19/006
摘要： An electrokinetic pump capable of producing high pressure is combined with a nozzle having a submicron orifice to provide a high pressure spray device. Because of its small size, the device can be contained within medical devices such as an endoscope for delivering biological materials such as DNA, chemo therapeutic agents, or vaccines to tissues and cells.
摘要翻译：能够产生高压的电动泵与具有亚微米孔的喷嘴组合以提供高压喷射装置。由于其体积小，装置可以包含在诸如用于将生物材料如DNA，化学治疗剂或疫苗递送到组织和细胞的内窥镜的医疗装置中。

5. 发明授权

US06843272B2 Conductance valve and pressure-to-conductance transducer method and apparatus 有权
标题翻译：电导阀和压力 - 电导传感器的方法和装置
公开(公告)号：US06843272B2
公开(公告)日：2005-01-18
申请号：US10304346
申请日：2002-11-25
申请人： Joseph S. Schoeniger , Eric B. Cummings , James S. Brennan
发明人： Joseph S. Schoeniger , Eric B. Cummings , James S. Brennan
IPC分类号： F15C5/00 , F16K99/00 , F15B1/04 , F15B21/00
CPC分类号： F16K99/0001 , F15C5/00 , F16K99/0017 , F16K99/0019 , F16K99/0057 , Y10T137/2076 , Y10T137/2082 , Y10T137/2191 , Y10T137/2196
摘要： A device for interrupting or throttling undesired ionic transport through a fluid network is disclosed. The device acts as a fluid valve by reversibly generating a fixed “bubble” in the conducting solvent solution carried by the network. The device comprises a porous hydrophobic structure filling a portion of a connecting channel within the network and optionally incorporates flow restrictor elements at either end of the porous structure that function as pressure isolation barriers, and a fluid reservoir connected to the region of the channel containing the porous structure. Also included is a pressure pump connected to the fluid reservoir. The device operates by causing the pump to vary the hydraulic pressure to a quantity of solvent solution held within the reservoir and porous structure. At high pressures, most or all of the pores of the structure are filled with conducting liquid so the ionic conductance is high. At lower pressures, only a fraction of the pores are filled with liquid, so ionic conductivity is lower. Below a threshold pressure, the porous structure contains only vapor, so there is no liquid conduction path. The device therefore effectively throttles ionic transport through the porous structure and acts as a “conductance valve” or “pressure-to-conductance” transducer within the network.
摘要翻译：公开了一种通过流体网络中断或节流不期望的离子传输的装置。该装置通过在网络所携带的导电溶剂溶液中可逆地产生固定的“气泡”而充当流体阀。该装置包括填充网络内的连接通道的一部分的多孔疏水结构，并且任选地在用作压力隔离屏障的多孔结构的任一端并入流体限制器元件，以及连接到通道的包含多孔结构。还包括连接到流体储存器的压力泵。该装置通过使泵将液压变化为保持在储存器和多孔结构内的溶剂溶液的量来操作。在高压下，结构的大部分或全部孔被导电液体填充，因此离子电导率高。在较低的压力下，只有一部分孔被液体填充，因此离子电导率较低。低于阈值压力，多孔结构仅含有蒸气，因此不存在液体传导路径。因此，该装置有效地阻止离子传输通过多孔结构并且在网络内充当“电导阀”或“压力 - 电导”传感器。

6. 发明授权

US06287440B1 Method for eliminating gas blocking in electrokinetic pumping systems 有权
标题翻译：在动力泵送系统中消除气体阻塞的方法
公开(公告)号：US06287440B1
公开(公告)日：2001-09-11
申请号：US09336535
申请日：1999-06-18
申请人： Don W. Arnold , Phillip H. Paul , Joseph S. Schoeniger
发明人： Don W. Arnold , Phillip H. Paul , Joseph S. Schoeniger
IPC分类号： B01D5702
CPC分类号： F04B53/06 , F04B19/006
摘要： A method for eliminating gas bubble blockage of current flow during operation of an electrokinetic pump. By making use of the ability to modify the surface charge on the porous dielectric medium used in electrokinetic pumps, it becomes possible to place electrodes away from the pressurized region of the electrokinetic pump. While gas is still generated at the electrodes they are situated such that the generated gas can escape into a larger buffer reservoir and not into the high pressure region of the pump where the gas bubbles can interrupt current flow. Various combinations of porous dielectric materials and ionic conductors can be used to create pumps that have desirable electrical, material handling, and flow attributes.
摘要翻译：一种在电动泵运行过程中消除气流阻塞电流的方法。通过利用在电动泵中使用的多孔介电介质上的表面电荷的修饰能力，可以将电极放置在电动泵的加压区域之外。虽然在电极处仍然产生气体，但是它们被定位成使得所产生的气体可以逸出到更大的缓冲容器中，而不会进入泵的高压区域，在该高压区域中气泡可以中断电流。可以使用多孔介电材料和离子导体的各种组合来产生具有期望的电气，材料处理和流动属性的泵。

7. 发明授权

US06277257B1 Electrokinetic high pressure hydraulic system 有权
标题翻译：电动高压液压系统
公开(公告)号：US06277257B1
公开(公告)日：2001-08-21
申请号：US09271687
申请日：1999-03-18
申请人： Phillip H. Paul , David J. Rakestraw , Don W. Arnold , Kenneth R. Hencken , Joseph S. Schoeniger , David W. Neyer
发明人： Phillip H. Paul , David J. Rakestraw , Don W. Arnold , Kenneth R. Hencken , Joseph S. Schoeniger , David W. Neyer
IPC分类号： B01D6144
CPC分类号： G01N30/32 , F04B19/006 , G01N2030/285 , G01N2030/326
摘要： An electrokinetic high pressure hydraulic pump for manipulating fluids in capillary-based systems. The pump uses electro-osmotic flow to provide a high pressure hydraulic system, having no moving mechanical parts, for pumping and/or compressing fluids, for providing valve means and means for opening and closing valves, for controlling fluid flow rate, and manipulating fluid flow generally and in capillary-based systems (Microsystems), in particular. The compact nature of the inventive high pressure hydraulic pump provides the ability to construct a micro-scale or capillary-based HPLC system that fulfills the desire for small sample quantity, low solvent consumption, improved efficiency, the ability to run samples in parallel, and field portability. Control of pressure and solvent flow rate is achieved by controlling the voltage applied to an electrokinetic pump.
摘要翻译：一种电动高压液压泵，用于在毛细管系统中操纵流体。泵使用电渗流提供高压液压系统，没有移动的机械部件，用于泵送和/或压缩流体，用于提供阀装置和用于打开和关闭阀门的装置，用于控制流体流速和操纵流体特别是在毛细管系统（Microsystems）中通常流动。本发明的高压液压泵的紧凑特性提供构建基于微量或基于毛细管的HPLC系统的能力，其满足对小样品量，低溶剂消耗，提高的效率，并行运行样品的能力的需求，以及现场可携带性。通过控制施加到电动泵的电压来实现压力和溶剂流速的控制。

8. 发明授权

US07368290B2 Structural determination of intact proteins using mass spectrometry 有权
标题翻译：使用质谱法测定完整蛋白质的结构
公开(公告)号：US07368290B2
公开(公告)日：2008-05-06
申请号：US10437268
申请日：2003-05-12
申请人： Gary Kruppa , Joseph S. Schoeniger , Malin M. Young
发明人： Gary Kruppa , Joseph S. Schoeniger , Malin M. Young
IPC分类号： G01N33/00 , G01N24/00
CPC分类号： G01N33/6848
摘要： The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination.Additionally, the preferred method of the present invention involves the determination of protein structures utilizing a top-down analysis of protein structures to search for covalent modifications. In the preferred method, intact proteins are ionized and fragmented within the mass spectrometer.
摘要翻译：本发明涉及确定蛋白质序列和结构的新方法。具体地，本发明允许分析质谱仪内的完整蛋白质。因此，在将蛋白质样品引入质谱仪之前，不需要进行准备分离。本文还公开了用于增强来自所需修饰蛋白质的信号，质谱仪中产生受控蛋白片段的方法，消除复杂微分离和用于基于交联的蛋白质结构测定所必需的蛋白质准备化学步骤的新的仪器开发。此外，本发明的优选方法涉及使用蛋白质结构的自顶向下分析来搜索共价修饰来确定蛋白质结构。在优选的方法中，完整的蛋白质在质谱仪内离子化并碎裂。

9. 发明授权

US06572749B1 Electrokinetic high pressure hydraulic system 有权
标题翻译：电动高压液压系统
公开(公告)号：US06572749B1
公开(公告)日：2003-06-03
申请号：US09912154
申请日：2001-07-23
申请人： Phillip H. Paul , David J. Rakestraw , Don W. Arnold , Kenneth R. Hencken , Joseph S. Schoeniger , David W. Neyer
发明人： Phillip H. Paul , David J. Rakestraw , Don W. Arnold , Kenneth R. Hencken , Joseph S. Schoeniger , David W. Neyer
IPC分类号： B05B500
CPC分类号： G01N30/32 , F04B19/006 , G01N2030/285 , G01N2030/326
摘要： An electrokinetic high pressure hydraulic pump for manipulating fluids in capillary-based system. The pump uses electro-osmotic flow to provide a high pressure hydraulic system, having no moving mechanical parts, for pumping and/or compressing fluids, for providing valve means and means for opening and closing valves, for controlling fluid flow rate, and manipulating fluid flow generally and in capillary-based systems (microsystems), in particular. The compact nature of the inventive high pressure hydraulic pump provides the ability to construct a micro-scale or capillary-based HPLC system that fulfills the desire for small sample quantity, low solvent consumption, improved efficiency, the ability to run samples in parallel, and field portability. Control of pressure and solvent flow rate is achieved by controlling the voltage applied to an electrokinetic pump.
摘要翻译：一种电动高压液压泵，用于在毛细管系统中操纵流体。泵使用电渗流提供高压液压系统，没有移动的机械部件，用于泵送和/或压缩流体，用于提供阀装置和用于打开和关闭阀门的装置，用于控制流体流速和操纵流体特别是在毛细管系统（微系统）中流动。本发明的高压液压泵的紧凑特性提供构建基于微量或基于毛细管的HPLC系统的能力，其满足对小样品量，低溶剂消耗，提高的效率，并行运行样品的能力的需求，以及现场可携带性。通过控制施加到电动泵的电压来实现压力和溶剂流速的控制。

10. 发明授权

US06495015B1 Electrokinetically pumped high pressure sprays 有权
标题翻译：电动泵送高压喷雾
公开(公告)号：US06495015B1
公开(公告)日：2002-12-17
申请号：US09595799
申请日：2000-06-16
申请人： Joseph S. Schoeniger , Phillip H. Paul , Luke Schoeniger
发明人： Joseph S. Schoeniger , Phillip H. Paul , Luke Schoeniger
IPC分类号： B05B500
CPC分类号： F04B17/00 , A61M37/00 , B05B5/00 , B05B5/025 , B05B5/1691 , F04B19/006
摘要： An electrokinetic pump capable of producing high pressure is combined with a nozzle having a submicron orifice to provide a high pressure spray device. Because of its small size, the device can be contained within medical devices such as an endoscope for delivering biological materials such as DNA, chemo therapeutic agents, or vaccines to tissues and cells.
摘要翻译：能够产生高压的电动泵与具有亚微米孔的喷嘴组合以提供高压喷射装置。由于其体积小，装置可以包含在诸如用于将生物材料如DNA，化学治疗剂或疫苗递送到组织和细胞的内窥镜的医疗装置中。

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