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1. 发明授权

US5955274A Detection of fungal pathogens using the polymerase chain reaction 失效
标题翻译：使用聚合酶链反应检测真菌病原体
公开(公告)号：US5955274A
公开(公告)日：1999-09-21
申请号：US722187
申请日：1996-10-15
申请人： James M. Ligon , James J. Beck
发明人： James M. Ligon , James J. Beck
IPC分类号： C12N15/09 , A01N63/04 , C12N1/14 , C12N15/80 , C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： DNA sequences from the Internal Transcribed Spacers of the ribosomal RNA gene region are described for different species and strains of Septoria, Pseudocercosporella, Fusarium and Mycosphaerella. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.
摘要翻译： PCT No.PCT / US95 / 04712 Sec。 371日期1996年10月15日 102（e）日期1996年10月15日PCT提交1995年4月19日PCT公布。公开号WO95 / 29260 PCT 1995年11月2日，对来自核糖体RNA基因区域的内部转录间隔区的DNA序列描述了不同的Septoria，Pseudocercosporella，Fusarium和Mycosphaerella的菌株。鉴定这些序列内的特异性引物可用于使用基于PCR的技术鉴定真菌分离物。

2. 发明授权

US5716849A Genes for the biosynthesis of soraphen 失效
标题翻译：索拉帕生物合成基因
公开(公告)号：US5716849A
公开(公告)日：1998-02-10
申请号：US764233
申请日：1996-12-14
申请人： James M. Ligon , Thomas Schupp , James J. Beck , Dwight S. Hill , Snezana Neff , John A. Ryals
发明人： James M. Ligon , Thomas Schupp , James J. Beck , Dwight S. Hill , Snezana Neff , John A. Ryals
IPC分类号： C12N15/52 , C12N1/20
CPC分类号： C12N15/52
摘要： The present invention is directed to the production of a polyketide antibiotic such as soraphen in a host via recombinant expression of the polypeptides needed to biologically synthesize the polyketide antibiotic. Polyketide synthase (PKS) genes encoding polypeptides necessary to synthesize soraphen are provided, along with methods for identifying and isolating the PKS genes needed to recombinantly biosynthesize any desired polyketide antibiotic. The cloned PKS genes may be transformed and expressed in a desired host organisms to produce soraphen for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of soraphen.
摘要翻译：本发明涉及通过重组表达生物合成聚酮化合物抗生素所需的多肽来在宿主中生产聚酮化合物抗生素例如索帕洛芬。提供了编码合成索拉非所需的多肽的聚酮化合物合酶（PKS）基因，以及用于鉴定和分离重组生物合成任何所需聚酮化合物抗生素所需的PKS基因的方法。克隆的PKS基因可以在所需的宿主生物体中转化和表达，以产生多种目的的索拉帕，包括保护宿主免受病原体的侵袭，将宿主作为生物防治剂开发，并产生大量均匀量的索拉帕芬。

3. 发明授权

US5585238A Detection of fungal pathogens using the polymerase chain reaction 失效
标题翻译：使用聚合酶链反应检测真菌病原体
公开(公告)号：US5585238A
公开(公告)日：1996-12-17
申请号：US233608
申请日：1994-04-25
申请人： James M. Ligon , James J. Beck
发明人： James M. Ligon , James J. Beck
IPC分类号： C12N15/09 , A01N63/04 , C12N1/14 , C12N15/80 , C12P19/34 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： DNA sequences from the Internal Transcribed Spacer of the ribosomal RNA gene region are described for different species and strains of Septoria, Pseudocercosporella and Mycosphaerella. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques.
摘要翻译：描述了来自核糖体RNA基因区域的内部转录间隔区的DNA序列用于不同种类和Septoria，Pseudocercosporella和Mycosphaerella菌株。鉴定这些序列内的特异性引物可用于使用基于PCR的技术鉴定真菌分离物。

4. 发明授权

US5942662A Inducible herbicide resistance 失效
标题翻译：诱导性除草剂抗性
公开(公告)号：US5942662A
公开(公告)日：1999-08-24
申请号：US971217
申请日：1997-11-14
申请人： John A. Ryals , Christian T. Harms , Leslie B. Friedrich , James J. Beck , Scott J. Uknes , Eric R. Ward
发明人： John A. Ryals , Christian T. Harms , Leslie B. Friedrich , James J. Beck , Scott J. Uknes , Eric R. Ward
IPC分类号： C07D285/14 , C07K14/415 , C12N9/02 , C12N9/08 , C12N9/24 , C12N9/36 , C12N15/29 , C12N15/56 , C12N15/62 , C12N15/82 , A01H5/00
CPC分类号： C12N9/2462 , C07D285/14 , C07K14/415 , C12N15/625 , C12N15/8238 , C12N15/8239 , C12N15/8274 , C12N15/8282 , C12N15/8286 , C12N9/0065 , C12N9/0073 , C12N9/2442 , C12Y114/13001 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058 , C12Y302/01073 , C07K2319/00 , C07K2319/02 , C07K2319/55 , C07K2319/61
摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.
摘要翻译：本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活其调节植物中的基因，并且在需要表达基因时在植物中施用化学调节剂还提供了新的信号肽序列和相应的DNA编码序列。进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

5. 发明授权

US5654414A Chemically inducible promoter of a cucumber chitinase/lysozyme gene 失效
标题翻译：黄瓜几丁质酶/溶菌酶基因的化学诱导型启动子
公开(公告)号：US5654414A
公开(公告)日：1997-08-05
申请号：US444803
申请日：1995-05-19
申请人： John A. Ryals , James J. Beck , Leslie B. Friedrich
发明人： John A. Ryals , James J. Beck , Leslie B. Friedrich
IPC分类号： C07D285/14 , C07K14/415 , C12N9/02 , C12N9/08 , C12N9/24 , C12N9/36 , C12N15/29 , C12N15/56 , C12N15/62 , C12N15/82 , A01H5/00
CPC分类号： C12N9/2462 , C07D285/14 , C07K14/415 , C12N15/625 , C12N15/8238 , C12N15/8239 , C12N15/8274 , C12N15/8282 , C12N15/8286 , C12N9/0065 , C12N9/0073 , C12N9/2442 , C12Y114/13001 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058 , C12Y302/01073 , C07K2319/00 , C07K2319/02 , C07K2319/55 , C07K2319/61
摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.
摘要翻译：本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活其调节植物中的基因，并且在需要表达基因的时候将化学调节剂施用于植物。还提供了新的信号肽序列和相应的DNA编码序列。进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

6. 发明授权

US5650505A Chemically regulatable and anti-pathogenic DNA sequences and uses thereof 失效
标题翻译：化学上可调节的和抗病原性的DNA序列及其用途
公开(公告)号：US5650505A
公开(公告)日：1997-07-22
申请号：US449315
申请日：1995-05-24
申请人： John A. Ryals , Danny C. Alexander , James J. Beck , John H. Duesing , Robert M. Goodman , Leslie B. Friedrich , Christian Harms , Frederich Meins, Jr. , Alice Montoya, deceased , Mary B. Moyer , Jean-Marc Neuhaus , George B. Payne , Christoph Sperisen , Jeffrey R. Stinson , Scott J. Uknes , Eric R. Ward , Shericca C. Williams
发明人： John A. Ryals , Danny C. Alexander , James J. Beck , John H. Duesing , Robert M. Goodman , Leslie B. Friedrich , Christian Harms , Frederich Meins, Jr. , Alice Montoya, deceased , Mary B. Moyer , Jean-Marc Neuhaus , George B. Payne , Christoph Sperisen , Jeffrey R. Stinson , Scott J. Uknes , Eric R. Ward , Shericca C. Williams
IPC分类号： C07D285/14 , C07K14/415 , C12N9/02 , C12N9/08 , C12N9/24 , C12N9/36 , C12N15/29 , C12N15/56 , C12N15/62 , C12N15/82 , A01H5/00
CPC分类号： C12N9/2462 , C07D285/14 , C07K14/415 , C12N15/625 , C12N15/8238 , C12N15/8239 , C12N15/8274 , C12N15/8282 , C12N15/8286 , C12N9/0065 , C12N9/0073 , C12N9/2442 , C12Y114/13001 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058 , C12Y302/01073 , C07K2319/00 , C07K2319/02 , C07K2319/55 , C07K2319/61
摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.
摘要翻译：本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活其调节植物中的基因，并且在需要表达基因的时候将化学调节剂施用于植物。还提供了新的信号肽序列和相应的DNA编码序列。进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

7. 发明授权

US5614395A Chemically regulatable and anti-pathogenic DNA sequences and uses thereof 失效
标题翻译：化学上可调节的和抗病原性的DNA序列及其用途
公开(公告)号：US5614395A
公开(公告)日：1997-03-25
申请号：US181271
申请日：1994-01-13
申请人： John A. Ryals , Danny C. Alexander , James J. Beck , John H. Duesing , Robert M. Goodman , Leslie B. Friedrich , Christian Harms , Frederich Meins, Jr. , Alice Montoya, deceased , Mary B. Moyer , Jean-Marc Neuhaus , George B. Payne , Christoph Sperisen , Jeffrey R. Stinson , Scott J. Uknes , Eric R. Ward , Shericca C. Williams
发明人： John A. Ryals , Danny C. Alexander , James J. Beck , John H. Duesing , Robert M. Goodman , Leslie B. Friedrich , Christian Harms , Frederich Meins, Jr. , Alice Montoya, deceased , Mary B. Moyer , Jean-Marc Neuhaus , George B. Payne , Christoph Sperisen , Jeffrey R. Stinson , Scott J. Uknes , Eric R. Ward , Shericca C. Williams
IPC分类号： C07D285/14 , C07K14/415 , C12N9/02 , C12N9/08 , C12N9/24 , C12N9/36 , C12N15/29 , C12N15/56 , C12N15/62 , C12N15/82 , C12N15/09
CPC分类号： C12N9/2462 , C07D285/14 , C07K14/415 , C12N15/625 , C12N15/8238 , C12N15/8239 , C12N15/8274 , C12N15/8282 , C12N15/8286 , C12N9/0065 , C12N9/0073 , C12N9/2442 , C12Y114/13001 , C12Y302/01014 , C12Y302/01039 , C12Y302/01058 , C12Y302/01073 , C07K2319/00 , C07K2319/02 , C07K2319/55 , C07K2319/61
摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or; at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.
摘要翻译：本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。还提供了一种用于外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族的植物，或; 至少一种异源基因，由于至少一种调节植物中基因的内源性信号转导级联的失活，以及在需要基因表达时将化学调节剂施用于植物。还提供了新的信号肽序列和相应的DNA编码序列。进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

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