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1. 发明授权

US07585641B2 Immunoassay and method of use 失效
标题翻译：免疫测定和使用方法
公开(公告)号：US07585641B2
公开(公告)日：2009-09-08
申请号：US10348078
申请日：2003-01-21
申请人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
发明人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
IPC分类号： C12N7/00 , C12N7/01 , C12N11/14 , C12N13/00 , C12Q1/06 , C12Q1/70 , G01N33/53
CPC分类号： G01N33/558 , G01N33/56911 , G01N2400/50
摘要： A method for performing an immunoassay is described. The method is particularly useful for detecting extracellular polysaccharide (EPS) and/or lipopolysaccharide (LPS) producing microorganisms. The method is particularly useful for detecting microorganisms which produce extracellular polysaccharides (EPS) also known as exocellular polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a preferred method for detecting microorganisms which produce EPS, LPS, or both, the EPS and/or LPS is extracted from a sample with cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates which are then preferentially bound to colored polystyrene latex particles over other components in the sample, and the bound EPS and/or LPS detected using a lateral flow immunoassay apparatus which has immobilized thereon antibodies specific for the EPS and/or LPS. The method can also be used to detect particular viruses, for example viruses of the potyviridae or tobamoviridae group.
摘要翻译：描述了进行免疫测定的方法。该方法特别适用于检测产生细胞外多糖（EPS）和/或脂多糖（LPS）的微生物。该方法特别可用于检测产生也被称为外细胞多糖，胶囊和/或脂多糖（LPS）的细胞外多糖（EPS）的微生物。在用于检测产生EPS，LPS或两者的微生物的优选方法中，用十六烷基三甲基溴化铵（CTAB）从样品中提取EPS和/或LPS以产生分子聚集体，然后优选与着色聚苯乙烯胶乳颗粒结合到其它组分上并且使用已经固定有EPS和/或LPS特异性抗体的侧流免疫测定装置检测结合的EPS和/或LPS。该方法还可以用于检测特定病毒，例如病毒科或鸟病毒科的病毒。

2. 发明申请

US20100041070A1 Immunoassay and method of use 审中-公开
标题翻译：免疫测定和使用方法
公开(公告)号：US20100041070A1
公开(公告)日：2010-02-18
申请号：US12459903
申请日：2009-07-09
申请人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
发明人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
IPC分类号： G01N33/543
CPC分类号： G01N33/558 , G01N33/56911 , G01N2400/50
摘要： A method for performing an immunoassay is described. The method is particularly useful for detecting extracellular polysaccharide (EPS) and/or lipopolysaccharide (LPS) producing microorganisms. The method is particularly useful for detecting microorganisms which produce extracellular polysaccharides (EPS) also known as exocellular polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a preferred method for detecting microorganisms which produce EPS, LPS, or both, the EPS and/or LPS is extracted from a sample with cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates which are then preferentially bound to colored polystyrene latex particles over other components in the sample, and the bound EPS and/or LPS detected using a lateral flow immunoassay apparatus which has immobilized thereon antibodies specific for the EPS and/or LPS. The method can also be used to detect particular viruses, for example viruses of the potyviridae or tobamoviridae group.
摘要翻译：描述了进行免疫测定的方法。该方法特别适用于检测产生细胞外多糖（EPS）和/或脂多糖（LPS）的微生物。该方法特别可用于检测产生也被称为外细胞多糖，胶囊和/或脂多糖（LPS）的细胞外多糖（EPS）的微生物。在用于检测产生EPS，LPS或两者的微生物的优选方法中，用十六烷基三甲基溴化铵（CTAB）从样品中提取EPS和/或LPS以产生分子聚集体，然后优选与着色聚苯乙烯胶乳颗粒结合到其它组分上并且使用已经固定有EPS和/或LPS特异性抗体的侧流免疫测定装置检测结合的EPS和/或LPS。该方法还可以用于检测特定病毒，例如病毒科或鸟病毒科的病毒。

3. 发明授权

US4108727A Method, composition and device for determining the specific gravity or osmolality of a liquid 失效
标题翻译：用于测定液体比重或重量克分子渗透压浓度的方法，组成和装置
公开(公告)号：US4108727A
公开(公告)日：1978-08-22
申请号：US716962
申请日：1976-08-23
申请人： Sisto Nicholas Stiso , Chester L. Sutula
发明人： Sisto Nicholas Stiso , Chester L. Sutula
IPC分类号： G01N9/00 , G01N13/04 , G01N31/14
CPC分类号： G01N9/00 , G01N13/04 , Y10S435/805
摘要： A method and device for determining the specific gravity or osmolality of a liquid containing a nonionic, ionizable solute are disclosed. The method comprises contacting the liquid with an ionizing agent, thus converting the solute to ionized species in the liquid, and contacting the liquid with test means capable of producing a detectable response, such as a color response, dependent upon the specific gravity or osmolality of the ionized liquid. The composition comprises an ionizing agent capable, upon contact with the liquid, of ionizing the solute therein, and a test means capable, upon contacting the liquid containing the solute in ionized form, of producing a detectable response which is a function of the specific gravity or osmolality of the liquid. The device comprises a carrier matrix incorporated with the test means and the ionizing agent.
摘要翻译：公开了一种用于测定含有非离子型可电离溶质的液体的比重或重量克分子渗透浓度的方法和装置。该方法包括使液体与电离剂接触，从而将溶质转化为液体中的离子化物质，并使液体与能够产生可检测反应（例如颜色响应）的测试装置接触，这取决于比重或渗透压电离液体。所述组合物包含能够在与液体接触时离子化其中的溶质的电离剂，以及测试装置，其能够在使含有离子化形式的溶质的液体接触时产生作为比重的函数的可检测响应或液体的重量摩尔渗透压浓度。该装置包括与测试装置和电离剂结合的载体矩阵。

4. 发明授权

US06517856B1 Insecticide resistance assay 失效
标题翻译：杀虫剂抗性测定
公开(公告)号：US06517856B1
公开(公告)日：2003-02-11
申请号：US09567319
申请日：2000-05-09
申请人： R. Michael Roe , Woodward D. Bailey , Fred Gould , George G. Kennedy , Chester L. Sutula
发明人： R. Michael Roe , Woodward D. Bailey , Fred Gould , George G. Kennedy , Chester L. Sutula
IPC分类号： A01N2534
CPC分类号： G01N33/48 , A01N25/006 , A23K50/90 , G01N33/5085 , G01N2333/325 , G01N2333/43552
摘要： Methods of assaying insects for pesticide resistance and to identify insect species are based on feeding disruption caused by insecticide such as the biopesticide Bacillius thuringiensis toxin (Bt). Further provided are methods for monitoring insecticide resistance in a population of insects, as well as methods of screening compounds for insecticidal activity. Insecticide resistance can be assessed at the level of an individual insect or at population levels. Apparatus and dehydrated insect meal pads useful in such assays are described.
摘要翻译：测定昆虫抗药性和鉴定昆虫物种的方法是基于杀虫剂引起的饲料破坏，如生物农药芽孢杆菌毒素（Bt）。还提供了用于监测昆虫群体中的杀虫剂抗性的方法以及筛选化合物的杀虫活性的方法。杀虫剂抗性可以在个别昆虫水平或群体水平进行评估。描述了可用于这种测定的装置和脱水的昆虫粉垫。

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