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1. 发明授权

US08303936B2 Optical fluorescent imaging 有权
标题翻译：光学荧光成像
公开(公告)号：US08303936B2
公开(公告)日：2012-11-06
申请号：US13525136
申请日：2012-06-15
申请人： Daniel R. Draney , Ananda G. Lugade , Narasimhachari Narayanan , Joy Kovar , D. Michael Olive
发明人： Daniel R. Draney , Ananda G. Lugade , Narasimhachari Narayanan , Joy Kovar , D. Michael Olive
IPC分类号： A61K49/00
CPC分类号： A61K49/0056 , A61K49/0032 , A61K49/0041 , C07D209/14 , C07D403/14 , C07D493/10 , C07K14/485 , C08B37/0012 , C09B23/0066
摘要： Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared (NIR) spectral range. The NIR is highly sensitive for tumor detection and tracking. The application discloses targeting a tumor-enriched cell surface receptor with a ligand-conjugated fluorescent probe, which specifically allows detection of the tumor relative to the negligible animal autofluorescence.
摘要翻译：公开了可用于近红外（NIR）光谱范围内的无创成像的化合物和方法。 NIR对肿瘤检测和跟踪非常敏感。该申请公开了用配体缀合的荧光探针靶向具有肿瘤的细胞表面受体，其特异性地允许相对于可忽略的动物自体荧光检测肿瘤。

2. 发明授权

US07795040B2 Methods, particles, and kits for determining activity of a kinase 失效
标题翻译：测定激酶活性的方法，粒子和试剂盒
公开(公告)号：US07795040B2
公开(公告)日：2010-09-14
申请号：US11736254
申请日：2007-04-17
申请人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
发明人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
IPC分类号： G01N33/543
CPC分类号： C12Q1/485
摘要： Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via a functional group of the support substrate. The method further includes phosphorylating the peptide substrate during exposure of the fluorescent particle to the assay and processing the fluorescent particle such that the peptide substrate is dephosphorylated and a polarized double bond is generated at a dephosphorylated site. In addition, the method includes coupling a fluorescent reporter having a nucleophilic terminal group to the fluorescent particle via the polarized double bond.
摘要翻译：提供了用于确定样品中激酶活性的方法，颗粒和试剂盒。一种方法的实施方案包括将荧光颗粒暴露于测定中，其中荧光颗粒包括具有一种或多种荧光材料的支撑衬底和通过支撑衬底的官能团与支撑衬底耦合的肽底物。该方法还包括在将荧光颗粒暴露于测定期间磷酸化底物，并处理荧光颗粒，使得肽底物脱磷酸化，并在去磷酸化位点产生偏振双键。此外，该方法包括通过偏振双键将具有亲核末端基团的荧光报告物偶联到荧光颗粒上。

3. 发明申请

US20130052637A1 OPTICAL FLUORESCENT IMAGING IN HISTOLOGY 审中-公开
标题翻译：光学荧光成像在组织学中的应用
公开(公告)号：US20130052637A1
公开(公告)日：2013-02-28
申请号：US13598583
申请日：2012-08-29
申请人： Joy Kovar , Jiyan Chen , Daniel R. Draney , D. Michael Olive , William M. Volcheck , Xinshe Xu , Ananda G. Lugade , Narasimhachari Narayanan
发明人： Joy Kovar , Jiyan Chen , Daniel R. Draney , D. Michael Olive , William M. Volcheck , Xinshe Xu , Ananda G. Lugade , Narasimhachari Narayanan
IPC分类号： G01N21/75 , G01N21/64
CPC分类号： A61K49/0056 , A61K49/0032 , A61K49/0041 , C07D209/14 , C07D403/14 , C07D493/10 , C07K14/485 , C08B37/0012 , C09B23/0066
摘要： Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared (NIR) spectral range. The NIR is highly sensitive for tumor detection and tracking. The application discloses targeting a tumor-enriched cell surface receptor with a ligand-conjugated fluorescent probe, which specifically allows detection of the tumor relative to the negligible animal autofluorescence.
摘要翻译：公开了可用于近红外（NIR）光谱范围内的无创成像的化合物和方法。 NIR对肿瘤检测和跟踪非常敏感。该申请公开了用配体缀合的荧光探针靶向具有肿瘤的细胞表面受体，其特异性地允许相对于可忽略的动物自体荧光检测肿瘤。

4. 发明授权

US08361169B2 Methods for forming dyed microspheres and populations of dyed microspheres 有权
标题翻译：形成染色微球体和染色微球体群体的方法
公开(公告)号：US08361169B2
公开(公告)日：2013-01-29
申请号：US13246486
申请日：2011-09-27
申请人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
发明人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
IPC分类号： C07C247/00
CPC分类号： C09B69/10 , C08F8/00 , C09B67/0097 , D06P1/008 , G01N33/585 , C08F212/08
摘要： Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.
摘要翻译：提供了形成染色微球的各种方法。一种方法包括使用热或光活化与染料偶联的化学结构，以在微球存在下形成反应中间体。反应中间体共价连接到微球的聚合物上，从而将染料偶联到聚合物上并形成染色的微球。提供用于形成耦合到分子的染色微球的其它方法。除了在染色的微球的外表面上合成分子之外，这些方法包括如上所述染色微球。还提供了一批染色微球。群体的每个染色的微球包括通过化学结构连接到每个染色微球的聚合物上的染料。由染料染色的微球体的染料特性的变异系数小于约10％。

5. 发明申请

US20120123026A1 FUNCTIONALIZED COMPOSITIONS FOR IMPROVED IMMOBILIZATION 有权
标题翻译：功能化组合物，用于改善固化
公开(公告)号：US20120123026A1
公开(公告)日：2012-05-17
申请号：US12119203
申请日：2008-05-12
申请人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
发明人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
IPC分类号： C08F212/36 , C08K9/12 , C08H1/00 , B82Y5/00
CPC分类号： B82Y30/00 , C07B2200/11 , C07C243/28 , C07C303/22 , C07C303/32 , C07C315/04 , C07C319/18 , C07C319/22 , C07C381/02 , C07C2601/04 , C07D239/48 , C07K1/042 , C07K17/00 , G01N33/54353 , Y10S977/902 , C07C309/47 , C07C309/88 , C07C309/46 , C07C317/18 , C07C323/65 , C07C323/42 , C07C309/42
摘要： The present invention relates to improved covalent coupling of two or more entities such as biomolecules, polymer compositions, organic/inorganic molecules/materials, and the like, including their combinations, through one or more novel reactive groups attached to linker groups of 2-1000 atoms length. The present invention also contemplates the use of bifunctional bridge molecules to link two or more entities, wherein the functional groups of the bridge molecules are the novel reactive groups of the present invention.
摘要翻译：本发明涉及两个或多个实体如生物分子，聚合物组合物，有机/无机分子/材料等的改进的共价偶联，包括其组合，通过连接到2-1000的连接基团上的一个或多个新的反应性基团原子长度。本发明还考虑使用双官能桥分子连接两个或多个实体，其中桥分子的官能团是本发明的新的反应性基团。

6. 发明授权

US08153440B2 Methods for altering surface characteristics of microspheres 有权
标题翻译：改变微球表面特性的方法
公开(公告)号：US08153440B2
公开(公告)日：2012-04-10
申请号：US12969863
申请日：2010-12-16
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： G01N21/75 , G01N37/00 , A61K9/16
CPC分类号： G01N21/645 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/586 , G01N2021/6421 , G01N2650/00 , Y10T436/13
摘要： Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.
摘要翻译：提供了改变微球表面特性的各种方法。一种方法包括将烯醇酸偶联到微球以改变微球的表面特性。表面特征可以包括电荷密度和/或pKa。可以通过烯醇酸将试剂偶联到微球。试剂可以包括生物分子。当试剂与微球耦合时，改性表面特性可增加试剂的稳定性。改进的表面特性还可以改善用微球进行的分析的性能。另一个实施方案涉及包含与微球的聚合物核心偶联的烯醇酸的微球，使得烯醇酸改性微球的表面特性。可以通过烯醇酸将试剂偶联到微球。

7. 发明申请

US20120035328A1 METHODS FOR FORMING DYED MICROSPHERES AND POPULATIONS OF DYED MICROSPHERES 有权
标题翻译：用于形成染色微球的人造粒的方法
公开(公告)号：US20120035328A1
公开(公告)日：2012-02-09
申请号：US13246486
申请日：2011-09-27
申请人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
发明人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
IPC分类号： C08F12/30 , C07D403/08 , C07C311/49
CPC分类号： C09B69/10 , C08F8/00 , C09B67/0097 , D06P1/008 , G01N33/585 , C08F212/08
摘要： Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.
摘要翻译：提供了形成染色微球的各种方法。一种方法包括使用热或光活化与染料偶联的化学结构，以在微球存在下形成反应中间体。反应中间体共价连接到微球的聚合物上，从而将染料偶联到聚合物上并形成染色的微球。提供用于形成耦合到分子的染色微球的其它方法。除了在染色的微球的外表面上合成分子之外，这些方法包括如上所述染色微球。还提供了一批染色微球。群体的每个染色的微球包括通过化学结构连接到每个染色微球的聚合物上的染料。由染料染色的微球体的染料特性的变异系数小于约10％。

8. 发明申请

US20110086431A1 Methods for Altering Surface Characteristics of Microspheres 有权
标题翻译：改变微球表面特性的方法
公开(公告)号：US20110086431A1
公开(公告)日：2011-04-14
申请号：US12969863
申请日：2010-12-16
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： G01N21/75
CPC分类号： G01N21/645 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/586 , G01N2021/6421 , G01N2650/00 , Y10T436/13
摘要： Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.
摘要翻译：提供了改变微球表面特性的各种方法。一种方法包括将烯醇酸偶联到微球以改变微球的表面特性。表面特征可以包括电荷密度和/或pKa。可以通过烯醇酸将试剂偶联到微球。试剂可以包括生物分子。当试剂与微球耦合时，改性表面特性可增加试剂的稳定性。改进的表面特性还可以改善用微球进行的分析的性能。另一个实施方案涉及包含与微球的聚合物核心偶联的烯醇酸的微球，使得烯醇酸改性微球的表面特性。可以通过烯醇酸将试剂偶联到微球。

9. 发明申请

US20100317544A1 Methods, Particles, and Kits for Determining Activity of a Kinase 审中-公开
标题翻译：用于确定激酶活性的方法，粒子和试剂盒
公开(公告)号：US20100317544A1
公开(公告)日：2010-12-16
申请号：US12861521
申请日：2010-08-23
申请人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
发明人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
IPC分类号： C12Q1/48 , C40B30/08 , C40B40/10
CPC分类号： C12Q1/485
摘要： Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via a functional group of the support substrate. The method further includes phosphorylating the peptide substrate during exposure of the fluorescent particle to the assay and processing the fluorescent particle such that the peptide substrate is dephosphorylated and a polarized double bond is generated at a dephosphorylated site. In addition, the method includes coupling a fluorescent reporter having a nucleophilic terminal group to the fluorescent particle via the polarized double bond.
摘要翻译：提供了用于确定样品中激酶活性的方法，颗粒和试剂盒。一种方法的实施方案包括将荧光颗粒暴露于测定中，其中荧光颗粒包括具有一种或多种荧光材料的支撑衬底和通过支撑衬底的官能团与支撑衬底耦合的肽底物。该方法还包括在将荧光颗粒暴露于测定期间磷酸化底物，并处理荧光颗粒，使得肽底物脱磷酸化，并在去磷酸化位点产生偏振双键。此外，该方法包括通过偏振双键将具有亲核末端基团的荧光报告物偶联到荧光颗粒上。

10. 发明申请

US20080103061A1 FUNCTIONALIZED COMPOSITIONS FOR IMPROVED IMMOBILIZATION 有权
标题翻译：功能化组合物，用于改善固化
公开(公告)号：US20080103061A1
公开(公告)日：2008-05-01
申请号：US11621209
申请日：2007-01-09
申请人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
发明人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
IPC分类号： C40B40/04 , C40B40/06 , C40B40/10
CPC分类号： B82Y30/00 , C07B2200/11 , C07C243/28 , C07C303/22 , C07C303/32 , C07C315/04 , C07C319/18 , C07C319/22 , C07C381/02 , C07C2601/04 , C07D239/48 , C07K1/042 , C07K17/00 , G01N33/54353 , Y10S977/902 , C07C309/47 , C07C309/88 , C07C309/46 , C07C317/18 , C07C323/65 , C07C323/42 , C07C309/42
摘要： The present invention relates to improved covalent coupling of two or more entities such as biomolecules, polymer compositions, organic/inorganic molecules/materials, and the like, including their combinations, through one or more novel reactive groups attached to linker groups of 2-1000 atoms length. The present invention also contemplates the use of bifunctional bridge molecules to link two or more entities, wherein the functional groups of the bridge molecules are the novel reactive groups of the present invention.
摘要翻译：本发明涉及两个或多个实体如生物分子，聚合物组合物，有机/无机分子/材料等的改进的共价偶联，包括其组合，通过连接到2-1000的连接基团上的一个或多个新的反应性基团原子长度。本发明还考虑使用双官能桥分子连接两个或多个实体，其中桥分子的官能团是本发明的新的反应性基团。

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