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1. 发明申请

US20100317544A1 Methods, Particles, and Kits for Determining Activity of a Kinase 审中-公开
标题翻译：用于确定激酶活性的方法，粒子和试剂盒
公开(公告)号：US20100317544A1
公开(公告)日：2010-12-16
申请号：US12861521
申请日：2010-08-23
申请人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
发明人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
IPC分类号： C12Q1/48 , C40B30/08 , C40B40/10
CPC分类号： C12Q1/485
摘要： Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via a functional group of the support substrate. The method further includes phosphorylating the peptide substrate during exposure of the fluorescent particle to the assay and processing the fluorescent particle such that the peptide substrate is dephosphorylated and a polarized double bond is generated at a dephosphorylated site. In addition, the method includes coupling a fluorescent reporter having a nucleophilic terminal group to the fluorescent particle via the polarized double bond.
摘要翻译：提供了用于确定样品中激酶活性的方法，颗粒和试剂盒。一种方法的实施方案包括将荧光颗粒暴露于测定中，其中荧光颗粒包括具有一种或多种荧光材料的支撑衬底和通过支撑衬底的官能团与支撑衬底耦合的肽底物。该方法还包括在将荧光颗粒暴露于测定期间磷酸化底物，并处理荧光颗粒，使得肽底物脱磷酸化，并在去磷酸化位点产生偏振双键。此外，该方法包括通过偏振双键将具有亲核末端基团的荧光报告物偶联到荧光颗粒上。

2. 发明授权

US07795040B2 Methods, particles, and kits for determining activity of a kinase 失效
标题翻译：测定激酶活性的方法，粒子和试剂盒
公开(公告)号：US07795040B2
公开(公告)日：2010-09-14
申请号：US11736254
申请日：2007-04-17
申请人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
发明人： James W. Jacobson , Ananda G. Lugade , Michaela R. Hoffmeyer
IPC分类号： G01N33/543
CPC分类号： C12Q1/485
摘要： Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via a functional group of the support substrate. The method further includes phosphorylating the peptide substrate during exposure of the fluorescent particle to the assay and processing the fluorescent particle such that the peptide substrate is dephosphorylated and a polarized double bond is generated at a dephosphorylated site. In addition, the method includes coupling a fluorescent reporter having a nucleophilic terminal group to the fluorescent particle via the polarized double bond.
摘要翻译：提供了用于确定样品中激酶活性的方法，颗粒和试剂盒。一种方法的实施方案包括将荧光颗粒暴露于测定中，其中荧光颗粒包括具有一种或多种荧光材料的支撑衬底和通过支撑衬底的官能团与支撑衬底耦合的肽底物。该方法还包括在将荧光颗粒暴露于测定期间磷酸化底物，并处理荧光颗粒，使得肽底物脱磷酸化，并在去磷酸化位点产生偏振双键。此外，该方法包括通过偏振双键将具有亲核末端基团的荧光报告物偶联到荧光颗粒上。

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