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1. 发明授权

US06902915B2 Heat-stable D-aminoacylase 失效
标题翻译：热稳定的D-氨基酸酰基酶
公开(公告)号：US06902915B2
公开(公告)日：2005-06-07
申请号：US10361509
申请日：2003-02-07
申请人： Shinji Tokuyama , Akinobu Matsuyama
发明人： Shinji Tokuyama , Akinobu Matsuyama
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/80 , C12N15/55 , C12P13/04 , C12P13/06 , C12P13/12 , C12P13/20 , C12P13/22 , C12R1/465 , C12N9/78 , C12P13/08
CPC分类号： C12R1/465 , C12N9/80 , C12P13/04
摘要： The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
摘要翻译：本发明提供了一种新的D-氨基酸酰基酶，以及使用其制备D-氨基酸的方法。为了实现上述目的，本发明人通过组合各种纯化方法成功地从属于链霉菌属的微生物纯化热稳定的D-氨基酰化酶。此外，本发明人发现纯化的热稳定性D-氨基酸酰基酶可用于工业生产D-氨基酸。通过利用热稳定的D-氨基酸酰化酶，可以容易且有效地从N-乙酰基-DL-氨基酸（例如，N-乙酰基-DL-甲硫氨酸，N-乙酰基-DL- 缬氨酸，N-乙酰基-DL-色氨酸，N-乙酰基-DL-苯丙氨酸，N-乙酰基-DL-丙氨酸，N-乙酰基-DL-亮氨酸等）。

2. 发明授权

US06780619B2 D-aminoacylase and gene encoding the same 失效
标题翻译： D-氨基酸酰化酶和编码相同的基因
公开(公告)号：US06780619B2
公开(公告)日：2004-08-24
申请号：US09770517
申请日：2001-01-26
申请人： Kazuya Mitsuhashi , Hiroaki Yamamoto , Akinobu Matsuyama , Shinji Tokuyama
发明人： Kazuya Mitsuhashi , Hiroaki Yamamoto , Akinobu Matsuyama , Shinji Tokuyama
IPC分类号： C12P1304
CPC分类号： C12N9/80 , C12P13/04 , C12P13/227 , C12P41/007
摘要： The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.
摘要翻译：本发明提供了源自嗜酸菌的D-氨基酸酰化酶编码基因，丝状真菌，由该基因编码的多肽及其同源物。本发明的D-氨基酰化酶能够从N-乙酰基-D-色氨酸产生D-色氨酸。 D-色氨酸可用作药用原料等。

3. 发明授权

US06514742B1 D-aminoacylases, method for producing the same, and method for producing D-amino acids using the same 失效
公开(公告)号：US06514742B1
公开(公告)日：2003-02-04
申请号：US09361901
申请日：1999-07-27
申请人： Kazuya Mitsuhashi , Hiroaki Yamamoto , Akinobu Matsuyama , Shinji Tokuyama
发明人： Kazuya Mitsuhashi , Hiroaki Yamamoto , Akinobu Matsuyama , Shinji Tokuyama
IPC分类号： C12N978
CPC分类号： C12P13/04 , C12N9/80 , C12P41/007
摘要： D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.

4. 发明授权

US5672504A Process for enriching an �R,S!-1,2-epoxide in one enantiomer by using microbes to convert one enantiomer to the other or to preferentially open the epoxide ring 失效
标题翻译：通过使用微生物将一种对映异构体转化为另一对映异构体或优先开环环氧环来富集一种对映异构体中的[R，S]
公开(公告)号：US5672504A
公开(公告)日：1997-09-30
申请号：US547902
申请日：1995-10-25
申请人： Akinobu Matsuyama , Yoshinori Kobayashi
发明人： Akinobu Matsuyama , Yoshinori Kobayashi
IPC分类号： C07D303/08 , C12P17/02 , C12P41/00
CPC分类号： C07D303/08 , C12P17/02 , C12P41/00 , Y10S435/823 , Y10S435/832 , Y10S435/834 , Y10S435/839 , Y10S435/849 , Y10S435/875 , Y10S435/877 , Y10S435/881 , Y10S435/917 , Y10S435/918 , Y10S435/92
摘要： A microorganism or a preparation thereof is permitted to act on a mixture of enantiomers of an epoxide such as 3-chlorostyrene oxide and the product optically active epoxide is recovered. The microorganism able to produce an optically active (S)-epoxide from the mixture of enantiomers of the epoxide include, for example, a microorganism strain belonging to the genus Candida, the genus Rhodosporidium, the genus Rhodococcus and the genus Nosardioides. Examples of the microorganism capable of producing an optically active (R)-epoxide from said mixture include a microorganism strain belonging to the genus Trichosporon, the genus Geotrichum, the genus Corynebacterium, the genus Micrococcus and the genus Brevibacterium. The objective optically active epoxide can efficiently be obtained with ease and simplicity from the corresponding mixture of enantiomers of the epoxide.
摘要翻译：允许微生物或其制剂作用于环氧化物的对映异构体如3-氯苯乙烯氧化物的混合物，并回收产物光学活性环氧化物。能够从环氧化物的对映异构体的混合物中产生光学活性的（S） - 环氧化物的微生物包括例如属于假丝酵母属，红球菌属，红球菌属和仙人掌属的微生物菌株。能够从所述混合物生产光学活性（R） - 环氧化物的微生物的实例包括属于Trichosporon属，Geotrichum属，棒状杆菌属，Micrococcus属和短杆菌属的微生物菌株。目的光学活性环氧化物可以从环氧化物的相应对映异构体的混合物中容易且简单地获得。

5. 发明授权

US5288620A Process for the production of optically active 2-hydroxy-4-phenyl-3-butenoic acid 失效
标题翻译：光学活性2-羟基-4-苯基-3-丁烯酸的制备方法
公开(公告)号：US5288620A
公开(公告)日：1994-02-22
申请号：US885974
申请日：1992-05-20
申请人： Akinobu Matsuyama , Ichiro Takase , Yoichiro Ueda , Yoshinori Kobayashi
发明人： Akinobu Matsuyama , Ichiro Takase , Yoichiro Ueda , Yoshinori Kobayashi
IPC分类号： C12P7/42 , C12R1/01 , C12R1/22 , C12R1/645
CPC分类号： C12P7/42 , Y10S435/822 , Y10S435/824 , Y10S435/827 , Y10S435/83 , Y10S435/836 , Y10S435/84 , Y10S435/843 , Y10S435/85 , Y10S435/852 , Y10S435/853 , Y10S435/857 , Y10S435/873 , Y10S435/874 , Y10S435/885 , Y10S435/911 , Y10S435/921 , Y10S435/93
摘要： Optically active 2-hydroxy-4-phenyl-3-butenoic acid can be obtained by treating 2-oxo-4-phenyl-3-butenoic acid with an optionally treated microorganism capable of asymmetrically reducing the 2-oxo-4-phenyl-3-butenoic acid into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid to thereby asymmetrically reduce the same into (R)-2-hydroxy-4-phenyl-3-butenoic acid or (S)-2-hydroxy-4-phenyl-3-butenoic acid.
摘要翻译：通过用任意处理的微生物处理2-氧代-4-苯基-3-丁烯酸可以获得光学活性的2-羟基-4-苯基-3-丁烯酸，其能够不对称地还原2-氧代-4-苯基-3-丁烯酸（R）-2-羟基-4-苯基-3-丁烯酸或（S）-2-羟基-4-苯基-3-丁烯酸，从而将其不对称地还原成（R）-2- 羟基-4-苯基-3-丁烯酸或（S）-2-羟基-4-苯基-3-丁烯酸。

6. 发明申请

US20120142050A1 NOVEL EXPRESSION VECTOR 审中-公开
标题翻译：新的表达向导
公开(公告)号：US20120142050A1
公开(公告)日：2012-06-07
申请号：US13144176
申请日：2010-01-13
申请人： Eitaro Matsumura , Momoko Hamasaki , Akinobu Matsuyama
发明人： Eitaro Matsumura , Momoko Hamasaki , Akinobu Matsuyama
IPC分类号： C12N1/21 , C12P21/00 , C12P1/04 , C12N15/74
CPC分类号： C12N15/74 , C12N9/0089 , C12N9/78 , C12Y115/01001 , C12Y305/05001
摘要： The present inventors constructed a transformation system which uses as a host the Kocuria rhizophila NBRC 103217 strain which can maintain its cellular structure in various organic solvents and shows little protein leakage.As a result, by developing novel shuttle vectors derived from Kocuria varians NBRC 15358, the present inventors successfully induced the expression of a foreign protein in microorganisms belonging to the genus Kocuria which can maintain their cellular structure in non-aqueous systems and show little protein leakage.
摘要翻译：本发明人构建了一种转化系统，其使用可以保持其在各种有机溶剂中的细胞结构并且几乎没有蛋白质渗漏的Kocuria rhizophila NBRC 103217菌株作为宿主。结果，通过开发由Kocuria变种NBRC 15358衍生的新的穿梭载体，本发明人成功诱导了属于Kocuria属的微生物中的外源蛋白的表达，其可以在非水系统中保持其细胞结构并且显示出很少的蛋白质渗漏。

7. 发明授权

US06255092B1 Stereospecific alcohol dehydrogenase isolated from Candida parapsilosis, amino acid and DNA sequences therefor, and method of preparation thereof 失效
标题翻译：从念珠菌分离的立体特异性醇脱氢酶，氨基酸及其DNA序列及其制备方法
公开(公告)号：US06255092B1
公开(公告)日：2001-07-03
申请号：US08855767
申请日：1997-06-11
申请人： Tomoko Kojima , Hiroaki Yamamoto , Naoki Kawada , Akinobu Matsuyama
发明人： Tomoko Kojima , Hiroaki Yamamoto , Naoki Kawada , Akinobu Matsuyama
IPC分类号： C12N904
CPC分类号： C12N9/0006 , C12P7/04 , C12P7/24 , C12P41/002
摘要： The present invention provides a novel secondary alcohol dehydrogenase useful for the synthesis of optically active alcohol and DNA encoding said enzyme. A microorganism belonging to genus Candida was found to produce a novel secondary alcohol dehydrogenase with a high stereochemical specificity. Using said enzyme, optically active alcohols were prepared, and by cloning of DNA encoding said enzyme, the base sequence of said DNA was determined. By providing a novel secondary alcohol dehydrogenase with a high stereochemical specificity and the gene encoding said enzyme, an efficient production of optically active alcohols became possible.
摘要翻译：本发明提供了可用于合成光学活性醇和编码所述酶的DNA的新型仲醇脱氢酶。发现属于念珠菌属的微生物产生具有高立体化学特异性的新型仲醇脱氢酶。使用所述酶制备光学活性醇，通过克隆编码所述酶的DNA，测定所述DNA的碱基序列。通过提供具有高立体化学特异性的新型仲醇脱氢酶和编码所述酶的基因，可以有效地制备光学活性醇。

8. 发明授权

US5916781A Method for producing D-tryptophan 失效
标题翻译： D-色氨酸的生产方法
公开(公告)号：US5916781A
公开(公告)日：1999-06-29
申请号：US5110
申请日：1998-01-09
申请人： Hiroaki Yamamoto , Kazuya Mitsuhashi , Akinobu Matsuyama , Fusao Tomita
发明人： Hiroaki Yamamoto , Kazuya Mitsuhashi , Akinobu Matsuyama , Fusao Tomita
IPC分类号： C12P13/22 , C12P41/00 , C12R1/01 , C12R1/05 , C12R1/15 , C12R1/18 , C12R1/19 , C12R1/22 , C12R1/37 , C12R1/40 , C12R1/425 , C12R1/64 , C12R1/84
CPC分类号： C12P41/001 , C12P13/227
摘要： The method for producing D-tryptophan with high optical purity and high yield is provided, which comprises contacting a mixture of D,L-tryptophan with organisms which produce tryptophanase to degrade L-tryptophan selectively, thereby increasing the content of D-tryptophan in D,L-tryptophan.
摘要翻译：提供了具有高光学纯度和高产率的D-色氨酸的制备方法，其包括使D，L-色氨酸的混合物与产生色氨酸酶的生物体接触以选择性降解L-色氨酸，从而增加D中的D-色氨酸含量，L-色氨酸。

9. 发明授权

US5811293A Processes for producing optically active 2-amino-1-phenylethanol derivatives by reduction of the corresponding ketone 失效
标题翻译：通过还原相应的酮制备光学活性2-氨基-1-苯基乙醇衍生物的方法
公开(公告)号：US5811293A
公开(公告)日：1998-09-22
申请号：US738864
申请日：1996-10-28
申请人： Kazuaki Furukawa , Takayuki Abe , Hidekazu Akamatsu , Akinobu Matsuyama , Michio Ito , Noritsugu Yamasaki , Katsuya Miki , Kiyoshi Ikura , Takeshi Ishiguro
发明人： Kazuaki Furukawa , Takayuki Abe , Hidekazu Akamatsu , Akinobu Matsuyama , Michio Ito , Noritsugu Yamasaki , Katsuya Miki , Kiyoshi Ikura , Takeshi Ishiguro
IPC分类号： C07C213/00 , C07C213/04 , C07C213/08 , C07C213/10 , C07C215/08 , C07C231/18 , C12P7/22 , C12P13/00 , C12P41/00
CPC分类号： C12P7/22 , C07C213/00 , C07C213/04 , C07C213/08 , C07C213/10 , C07C231/18 , C12P13/001 , C12P13/008 , C12P41/00 , C12P41/001 , C12P41/002 , Y10S435/822 , Y10S435/911
摘要： An (R)-2-amino-1-phenylethanol derivative shown by the general formula (IIa) ##STR1## wherein R.sup.1 and R.sup.5 represent a hydrogen atom, etc.; R.sup.2, R.sup.3 and R.sup.4 independently represent a halogen atom, etc., or a salt thereof, can readily be produced (1) by permitting a microorganism belonging to the genus Rhodosporidium, the genus Comamonas or the like to act on a mixture of corresponding (R)-form and (S)-form to asymmetrically utilize, or (2) by permitting a microorganism belonging to the genus Lodderomyces, the genus Pilimelia or the like to act on a corresponding aminoketone derivative to asymmetrically reduce. An (R,R)-1-phenyl-2-�(2-phenyl-1-alkylethyl) amino!ethanol derivative having a high optical purity can easily be obtained from the compound of the formula (IIa) or a salt thereof. Said derivative is useful as an intermediate for producing an anti-obesity agent and so on.
摘要翻译：由通式（IIa）表示的（R）-2-氨基-1-苯基乙醇衍生物（IIa）其中R1和R5表示氢原子等; R2，R3和R4独立地表示卤素原子等，或其盐可以容易地制备（1）通过使属于Rhodosporidium属，Comamonas属等的微生物作用于相应的（或者（2）通过允许属于罗德氏菌属的属微生物，类风云属等的微生物作用于相应的氨基酮衍生物以不对称地还原。具有高光学纯度的（R，R）-1-苯基-2 - [（2-苯基-1-烷基乙基）氨基]乙醇衍生物可以容易地从式（IIa）化合物或其盐获得。所述衍生物可用作生产抗肥胖剂等的中间体。

10. 发明授权

US5629200A Production of optically active 2-amino-1-phenylethanol derivatives by asymetrical assimilation 失效
标题翻译：通过非对称同化生产光学活性2-氨基-1-苯基乙醇衍生物
公开(公告)号：US5629200A
公开(公告)日：1997-05-13
申请号：US343952
申请日：1994-11-17
申请人： Kazuaki Furukawa , Takayuki Abe , Hidekazu Akamatsu , Akinobu Matsuyama , Michio Ito , Noritsugu Yamasaki , Katsuya Miki , Kiyoshi Ikura , Takeshi Ishiguro
发明人： Kazuaki Furukawa , Takayuki Abe , Hidekazu Akamatsu , Akinobu Matsuyama , Michio Ito , Noritsugu Yamasaki , Katsuya Miki , Kiyoshi Ikura , Takeshi Ishiguro
IPC分类号： C07C213/00 , C07C213/04 , C07C213/08 , C07C213/10 , C07C215/08 , C07C231/18 , C12P7/22 , C12P13/00 , C12P41/00
CPC分类号： C12P7/22 , C07C213/00 , C07C213/04 , C07C213/08 , C07C213/10 , C07C231/18 , C12P13/001 , C12P13/008 , C12P41/00 , C12P41/001 , C12P41/002 , Y10S435/822 , Y10S435/911
摘要： An (R)-2-amino-1-phenylethanol derivative shown by the general formula (IIa) ##STR1## wherein R.sup.1 and R.sup.5 represent a hydrogen atom, etc.; R.sup.2, R.sup.3 and R.sup.4 independently represent a halogen atom, etc., or a salt thereof, can readily be produced (1) by permitting a microorganism to act on a mixture of corresponding (R)-form and (S)-form to asymmetrically utilize, or (2) by permitting a microorganism to act on a corresponding aminoketone derivative to asymmetrically reduce. An (R,R)-1-phenyl-2-[(2-phenyl-1-alkylethyl) amino]ethanol derivative having a high optical purity can easily be obtained from the compound of the formula (IIa) or a salt thereof. Said derivative is useful as an intermediate for producing an anti-obesity agent.
摘要翻译：由通式（IIa）表示的（R）-2-氨基-1-苯基乙醇衍生物（IIa）其中R1和R5表示氢原子等; R2，R3和R4独立地表示卤素原子等，或其盐可容易地制备（1）通过允许微生物作用于相应的（R）形式和（S）形式的混合物到不对称的利用或（2）通过允许微生物作用于相应的氨基酮衍生物以不对称地还原。具有高光学纯度的（R，R）-1-苯基-2 - [（2-苯基-1-烷基乙基）氨基]乙醇衍生物可以容易地从式（IIa）化合物或其盐获得。所述衍生物可用作制备抗肥胖剂的中间体。

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