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71. 发明申请

US20050118578A1 Amplified nucleic acids and immobilized products thereof 审中-公开
标题翻译：扩增核酸及其固定化产物
公开(公告)号：US20050118578A1
公开(公告)日：2005-06-02
申请号：US10467325
申请日：2002-02-06
申请人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
发明人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/518 , C12Q2531/119
摘要： A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.
摘要翻译：通过使用嵌合寡核苷酸引物通过核酸扩增反应大量提供的核酸以含有修饰的脱氧核糖核苷酸的状态构建，用于将核酸固定至固相，然后固定于固体相，从而得到具有优异品质的固定化核酸产物。

72. 发明申请

US20050042208A1 Method of extended culture for antigen-specific cytotoxic lumphocytes 失效
标题翻译：抗原特异性细胞毒性淋巴细胞扩增培养方法
公开(公告)号：US20050042208A1
公开(公告)日：2005-02-24
申请号：US10486512
申请日：2002-08-15
申请人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
发明人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
IPC分类号： A61K39/395 , A61P31/12 , A61P31/22 , A61P35/00 , A61P37/04 , C07K14/78 , C12N5/02 , C12N5/07 , C12N5/0783 , C12N15/09 , C12N5/08
CPC分类号： C12N5/0636 , A61K2039/515 , A61K2039/5158 , C12N2501/105 , C12N2501/12 , C12N2501/23 , C12N2501/515 , C12N2501/58 , C12N2501/599 , C12N2501/905
摘要： The present invention is a method for inducing cytotoxic T cell having an antigen-specific cytotoxic activity, a method for maintaining the cell, a method for continuously culturing the cell or a method for expanding the cell, comprising the step of culturing a cytotoxic T cell in the presence of at least one substance selected from the group consisting of (A) a substance having a binding activity to CD44; (B) a substance capable of regulating a signal emitted by binding a CD44 ligand to CD44; (C) a substance capable of inhibiting binding of a growth factor to a growth factor receptor; (D) a substance capable of regulating a signal emitted by binding of a growth factor to a growth factor receptor; and (E) fibronectin, a fragment thereof or a mixture thereof.
摘要翻译：本发明是诱导具有抗原特异性细胞毒活性的细胞毒性T细胞的方法，用于维持细胞的方法，连续培养细胞的方法或扩增细胞的方法，包括培养细胞毒性T细胞的步骤在至少一种选自（A）与CD44具有结合活性的物质的物质的存在下，（B）能够调节通过将CD44配体结合CD44而发射的信号的物质; （C）能够抑制生长因子与生长因子受体结合的物质; （D）能够调节由生长因子与生长因子受体结合而发出的信号的物质; 和（E）纤连蛋白，其片段或其混合物。

73. 发明授权

US07910368B2 Method of extended culture for antigen-specific cytotoxic lymphocytes 失效
标题翻译：抗原特异性细胞毒性淋巴细胞扩增培养方法
公开(公告)号：US07910368B2
公开(公告)日：2011-03-22
申请号：US10486512
申请日：2002-08-15
申请人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
发明人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
IPC分类号： C12N5/08 , C12N5/00
CPC分类号： C12N5/0636 , A61K2039/515 , A61K2039/5158 , C12N2501/105 , C12N2501/12 , C12N2501/23 , C12N2501/515 , C12N2501/58 , C12N2501/599 , C12N2501/905
摘要： The present invention is a method for inducing cytotoxic T cell having an antigen-specific cytotoxic activity, a method for maintaining the cell, a method for continuously culturing the cell or a method for expanding the cell, comprising the step of culturing a cytotoxic T cell in the presence of at least one substance selected from the group consisting of (A) a substance having a binding activity to CD44; (B) a substance capable of regulating a signal emitted by binding a CD44 ligand to CD44; (C) a substance capable of inhibiting binding of a growth factor to a growth factor receptor; (D) a substance capable of regulating a signal emitted by binding of a growth factor to a growth factor receptor; and (E) fibronectin, a fragment thereof or a mixture thereof.
摘要翻译：本发明是诱导具有抗原特异性细胞毒活性的细胞毒性T细胞的方法，用于维持细胞的方法，连续培养细胞的方法或扩增细胞的方法，包括培养细胞毒性T细胞的步骤在至少一种选自（A）与CD44具有结合活性的物质的物质的存在下，（B）能够调节通过将CD44配体结合CD44而发射的信号的物质; （C）能够抑制生长因子与生长因子受体结合的物质; （D）能够调节由生长因子与生长因子受体结合而发出的信号的物质; 和（E）纤连蛋白，其片段或其混合物。

74. 发明授权

US07816134B2 Method of extensive culture of antigen-specific cytotoxic T cells 失效
标题翻译：广泛培养抗原特异性细胞毒性T细胞的方法
公开(公告)号：US07816134B2
公开(公告)日：2010-10-19
申请号：US10344534
申请日：2001-08-15
申请人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
发明人： Hiroaki Sagawa , Mitsuko Ideno , Ikunoshin Kato
IPC分类号： C12N5/08 , C12N5/00 , C12N5/02
CPC分类号： C12N5/0636 , A61K39/0011 , A61K39/12 , A61K39/145 , A61K2039/5158 , A61K2039/55583 , C12N2501/515 , C12N2501/90 , C12N2760/16134
摘要： The present invention provides methods for inducing, maintaining and expanding CTL (cytotoxic T cell) having an antigen-specific cytotoxic activity at a high level, which is useful in the adoptive immunotherapy, by using as an effective ingredient at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof. The above-mentioned compounds include fucoidans, heparins, alginic acid, chondroitin sulfate A, chondroitin sulfate B, pectic acid, hyaluronic acid, degradation products of fucoidans, sulfated glucose, sulfated fucose and salts thereof.
摘要翻译：本发明提供用于诱导，维持和扩增具有高水平的抗原特异性细胞毒性活性的CTL（细胞毒性T细胞）的方法，其可用于过继性免疫治疗，通过使用至少一种选自以下的化合物作为有效成分：由酸性多糖，酸性寡糖，酸性单糖及其盐组成的组。上述化合物包括岩藻多糖，肝素，海藻酸，硫酸软骨素A，硫酸软骨素B，果胶酸，透明质酸，岩藻多糖的降解产物，硫酸化葡萄糖，硫酸岩藻糖及其盐。

75. 发明授权

US07622291B2 Agarase and gene thereof 失效
标题翻译：琼脂糖酶及其基因
公开(公告)号：US07622291B2
公开(公告)日：2009-11-24
申请号：US11948650
申请日：2007-11-30
申请人： Jun Tomono , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C07H21/04 , C12N1/21 , C12N15/63 , C12P19/26 , C12N9/24 , C12N9/40
CPC分类号： C12Y302/01081 , C12N9/2468
摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.
摘要翻译：高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

76. 发明申请

US20070037139A1 Method of analyzing gene introduction site 审中-公开
标题翻译：分析基因引入位点的方法
公开(公告)号：US20070037139A1
公开(公告)日：2007-02-15
申请号：US10555876
申请日：2004-05-07
申请人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12Q1/70 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q2527/107
摘要： A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.
摘要翻译：一种在其中并入有逆转录病毒载体的染色体DNA中分析衍生自与载体相邻的染色体的DNA区域的方法，其特征在于引物延伸反应和核酸扩增反应在具有能力的化合物存在下进行降低双链核酸的Tm值。此外还提供了用于该方法的试剂盒和缓冲液。

77. 发明申请

US20060160219A1 Method of transferring mutation into target nucleic acid 审中-公开
标题翻译：将突变转移到靶核酸的方法
公开(公告)号：US20060160219A1
公开(公告)日：2006-07-20
申请号：US10520008
申请日：2003-07-11
申请人： Chunyu Cao , Hiroaki Sagawa , Ikunoshin Kato
发明人： Chunyu Cao , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N15/63
CPC分类号： C07K14/43595 , C12N15/102
摘要： A method of transferring a mutation into the base sequence of a target nucleic acid characterized by comprising the step of preparing a DNA having a reversed repetitive sequence wherein the base sequence of the DNA having the reversed repetitive sequence is homologous with the target nucleic acid and has a base sequence containing the mutation to be transferred into the target nucleic acid, and the step of transferring the DNA having the reversed repetitive sequence into cells; and a kit for the method.
摘要翻译：将突变转移到靶核酸的碱基序列中的方法，其特征在于包括制备具有反向重复序列的DNA的步骤，其中具有反向重复序列的DNA的碱基序列与靶核酸同源且具有含有要转移到靶核酸中的突变的碱基序列和将具有反向重复序列的DNA转移到细胞中的步骤; 和一个方法的工具包。

78. 发明申请

US20060051316A1 Remedy 审中-公开
标题翻译：补救
公开(公告)号：US20060051316A1
公开(公告)日：2006-03-09
申请号：US10537333
申请日：2003-12-05
申请人： Hiromu Ohnogi , Katsumi Sugiyama , Hiroaki Sagawa , Ikunoshin Kato
发明人： Hiromu Ohnogi , Katsumi Sugiyama , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： A61K31/74 , A61K31/737 , A61K31/727 , A61K31/715
CPC分类号： A61K36/02 , A23L33/10 , A61K31/216 , A61K31/737 , A61K31/78
摘要： The present invention relates to a therapeutic agent or prophylactic agent for a disease requiring enhancement of bone morphogenetic protein production or promotion of osteogenesis; an agent for enhancement of bone morphogenetic protein production or an agent for promotion of osteogenesis, a food, beverage or feed for enhancement of bone mornhogenetic protein production or promotion of osteogenesis, characterized in that each comprises as an effective ingredient at least one compound selected from the group consisting of (a) an acidic saccharide, (b) a polyacrylic acid, (c) chlorogenic acid and (d) an oxidation processed product of chlorogenic acid, or an extract derived from algae.
摘要翻译：本发明涉及需要增强骨形态发生蛋白产生或促进成骨的疾病的治疗剂或预防剂; 用于增强骨形态发生蛋白质产生的药物或用于促进成骨的药剂，用于增强骨发生蛋白质产生或促进成骨的食品，饮料或饲料，其特征在于，每种药物包含作为有效成分的至少一种选自由（a）酸性糖，（b）聚丙烯酸，（c）绿原酸和（d）绿原酸的氧化加工产物或源自藻类的提取物组成的组。

79. 发明授权

US06770481B1 Gene isolation method 失效
标题翻译：基因分离方法
公开(公告)号：US06770481B1
公开(公告)日：2004-08-03
申请号：US09690885
申请日：2000-10-18
申请人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
发明人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
IPC分类号： C12N1563
CPC分类号： C12N15/69 , C12N9/1007 , C12N9/22
摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.
摘要翻译：一种质粒载体，其特征在于包含可以通过RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和复制起点，其通过外源因子诱导的拷贝数增加; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法即使在不存在修饰酶基因的情况下也构成即将分离的限制性修饰系统的内切核酸酶 - 基因，这在现有技术中是困难的。

80. 发明授权

US06518317B1 Antiviral agents 失效
标题翻译：抗病毒剂
公开(公告)号：US06518317B1
公开(公告)日：2003-02-11
申请号：US09367185
申请日：1999-08-09
申请人： Hiroaki Sagawa , Nobuto Koyama , Hideto Chono , Kazutoh Takesako , Ikunoshin Kato
发明人： Hiroaki Sagawa , Nobuto Koyama , Hideto Chono , Kazutoh Takesako , Ikunoshin Kato
IPC分类号： A61K31122
CPC分类号： C07C323/58 , A23K20/105 , A23L2/52 , A23L33/10 , A61K31/122 , C07C323/59 , Y10S514/894
摘要： An antiviral agent which is characterized in containing at least one compound selected from a group consisting of 4,5-dihydroxy-2-cyclopenten-1-one represented by the following formula [I] and an optically active substance and a salt thereof as an effective component.
摘要翻译：一种抗病毒剂，其特征在于含有至少一种选自由下式[I]表示的4,5-二羟基-2-环戊烯-1-酮的化合物和光学活性物质及其盐作为有效成分。

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