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1. 发明授权

US6165749A Plasmid 失效
标题翻译：质粒
公开(公告)号：US6165749A
公开(公告)日：2000-12-26
申请号：US952089
申请日：1997-11-10
申请人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
发明人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N9/16 , C12N9/22 , C12N15/55 , C12N15/69 , C12R1/19 , C12P21/06
CPC分类号： C12N15/69 , C12N9/1007 , C12N9/22
摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.
摘要翻译： PCT No.PCT / JP97 / 00748 Sec。 371日期：1997年11月10日 102（e）日期1997年11月10日PCT 1997年3月10日PCT公布。第WO97 / 34006号公报日期1997年9月18日一种质粒载体，其特征在于包含可由RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和增加诱导下拷贝数的复制起点由外生因素; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法构成即使在不存在修饰酶基因的情况下分离的限制性修饰系统的核酸内切酶基因，这在现有技术中是困难的。

2. 发明授权

US06770481B1 Gene isolation method 失效
标题翻译：基因分离方法
公开(公告)号：US06770481B1
公开(公告)日：2004-08-03
申请号：US09690885
申请日：2000-10-18
申请人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
发明人： Hiroaki Sagawa , Harumi Ueno , Atsushi Oshima , Ikunoshin Kato
IPC分类号： C12N1563
CPC分类号： C12N15/69 , C12N9/1007 , C12N9/22
摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.
摘要翻译：一种质粒载体，其特征在于包含可以通过RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和复制起点，其通过外源因子诱导的拷贝数增加; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法即使在不存在修饰酶基因的情况下也构成即将分离的限制性修饰系统的内切核酸酶 - 基因，这在现有技术中是困难的。

3. 发明申请

US20070218524A1 Polypeptide Having Rnase III Activity 审中-公开
标题翻译：具有Rnase III活性的多肽
公开(公告)号：US20070218524A1
公开(公告)日：2007-09-20
申请号：US10573381
申请日：2004-09-29
申请人： Jun Tomono , Harumi Ueno , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Harumi Ueno , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12P21/00 , C07H21/02 , C07K2/00
CPC分类号： C12N15/111 , C12N9/22 , C12N2310/14 , C12N2330/30 , C12Y301/26003
摘要： A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.
摘要翻译：可以根据反应条件容易地控制dsRNA降解产物的长度的具有RNase III活性的多肽，并且在制备具有允许其在RNA干扰中作为siRNA的长度的dsRNA时，低分子量产物几乎没有形成RNA干扰效果; 使用上述多肽降解dsRNA的方法; 以及用于上述方法的组合物和试剂盒。

4. 发明申请

US20070105113A1 Methods of degrading dsrna and synthesizing rna 审中-公开
标题翻译：降解dsrna并合成rna的方法
公开(公告)号：US20070105113A1
公开(公告)日：2007-05-10
申请号：US10567731
申请日：2004-08-10
申请人： Hiroaki Sagawa , Jun Tomono , Harumi Ueno , Ikunoshin Kato
发明人： Hiroaki Sagawa , Jun Tomono , Harumi Ueno , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/22
CPC分类号： C12N9/22 , C12P19/34
摘要： A protein having an activity of degrading a dsRNA, namely, being capable of acting on a long-chain dsRNA to form a dsRNA of a definite length; a method of efficiently preparing a dsRNA of a definite length which comprises treating a dsRNA with the protein having an activity of degrading a dsRNA in the coexistence of a protein having an activity of binding to a nucleic acid such as a protein having an RNA-binding activity; and a method of using the protein having an activity of binding to a nucleic acid to elevate the efficiency in an RNA synthesis reaction typified by dsRNA synthesis.
摘要翻译：具有降解dsRNA活性的蛋白质，即能够作用于长链dsRNA以形成一定长度的dsRNA; 一种有效制备一定长度的dsRNA的方法，该方法包括在具有与核酸如具有RNA结合性的蛋白质结合的蛋白质共存的蛋白质共同存在下具有降解dsRNA活性的蛋白质对dsRNA进行处理活动; 以及使用具有与核酸结合的活性的蛋白质以提高以dsRNA合成为代表的RNA合成反应中的效率的方法。

5. 发明申请

US20050202425A1 Sulfated fucogalactan digesting enzyme gene 失效
标题翻译：硫酸岩藻糖激酶消化酶基因
公开(公告)号：US20050202425A1
公开(公告)日：2005-09-15
申请号：US10487097
申请日：2002-09-05
申请人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
发明人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
IPC分类号： C12N1/19 , C12N1/21 , C12N9/24 , C12N9/40 , C12N15/56 , C12P19/00 , C12P19/04 , C12P19/12 , C12P19/14 , C12Q1/68 , C07H21/04 , C12N15/74
CPC分类号： C12N9/2465 , C12N9/2402 , C12P19/00 , C12P19/04 , C12P19/12 , C12P19/14 , G01N2333/924
摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.
摘要翻译：编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析硫酸化岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

6. 发明授权

US07217552B2 Sulfated fucogalactan digesting enzyme gene 失效
标题翻译：硫酸岩藻糖激酶消化酶基因
公开(公告)号：US07217552B2
公开(公告)日：2007-05-15
申请号：US10487097
申请日：2002-09-05
申请人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
发明人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
IPC分类号： C12N9/24 , C12N1/20 , C12Q1/68 , C12P19/04 , C12P21/06 , C07H21/04
CPC分类号： C12N9/2465 , C12N9/2402 , C12P19/00 , C12P19/04 , C12P19/12 , C12P19/14 , G01N2333/924
摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.
摘要翻译：编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析含硫酸岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

7. 发明授权

US07410786B2 Sulfated fucogalactan digesting enzyme gene 失效
标题翻译：硫酸岩藻糖激酶消化酶基因
公开(公告)号：US07410786B2
公开(公告)日：2008-08-12
申请号：US11681453
申请日：2007-03-02
申请人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
发明人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
IPC分类号： C12N9/24 , C12N1/20 , C12P19/04 , C12P21/06 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/2465 , C12N9/2402 , C12P19/00 , C12P19/04 , C12P19/12 , C12P19/14 , G01N2333/924
摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.
摘要翻译：编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析硫酸化岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

8. 发明授权

US07244588B2 Cold-induced expression vector 失效
标题翻译：冷诱导表达载体
公开(公告)号：US07244588B2
公开(公告)日：2007-07-17
申请号：US10538793
申请日：2003-12-11
申请人： Jun Tomono , Harumi Ueno , Masayuni Kishimoto , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Harumi Ueno , Masayuni Kishimoto , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12N15/00 , C12P21/06 , C07K1/00
CPC分类号： C12N15/67 , C12N15/63
摘要： A vector having a region encoding a cold shock protein gene mRNA-origin 5′-nontranslated region, characterized in that the 5′-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.
摘要翻译：具有编码冷激蛋白基因mRNA起始的5'-非翻译区的区域的载体，其特征在于，所述5'-非翻译区具有转移的突变，以便改变由所述区域形成的茎结构的距离。

9. 发明申请

US20070148752A1 SULFATED FUCOGALACTAN DIGESTING ENZYME GENE 失效
标题翻译：硫化FUCOGALACTAN DIGESTING ENZYME基因
公开(公告)号：US20070148752A1
公开(公告)日：2007-06-28
申请号：US11681453
申请日：2007-03-02
申请人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
发明人： Harumi Ueno , Jun Tomono , Hiroaki Sagawa , Takeshi Sakai , Ikunoshin Kato
IPC分类号： C12N9/24 , C12P21/06 , C12N1/21 , C12N15/74
CPC分类号： C12N9/2465 , C12N9/2402 , C12P19/00 , C12P19/04 , C12P19/12 , C12P19/14 , G01N2333/924
摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.
摘要翻译：编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析含硫酸岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

10. 发明申请

US20070037139A1 Method of analyzing gene introduction site 审中-公开
标题翻译：分析基因引入位点的方法
公开(公告)号：US20070037139A1
公开(公告)日：2007-02-15
申请号：US10555876
申请日：2004-05-07
申请人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
发明人： Jun Tomono , Harumi Ueno , Osamu Takeda , Hiroyuki Mukai , Hiroaki Sagawa , Ikunoshin Kato
IPC分类号： C12Q1/70 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q2527/107
摘要： A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.
摘要翻译：一种在其中并入有逆转录病毒载体的染色体DNA中分析衍生自与载体相邻的染色体的DNA区域的方法，其特征在于引物延伸反应和核酸扩增反应在具有能力的化合物存在下进行降低双链核酸的Tm值。此外还提供了用于该方法的试剂盒和缓冲液。

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