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1. 发明申请

US20130295576A1 METHOD FOR MODIFYING NUCLEIC ACIDS 有权
标题翻译：修饰核酸的方法
公开(公告)号：US20130295576A1
公开(公告)日：2013-11-07
申请号：US13980970
申请日：2012-01-20
申请人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
发明人： Junko Yamamoto , Keiko Kubo , Takashi Uemori , Hiroyuki Mukai , Kiyozo Asada
IPC分类号： C12Q1/68
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及：用于修饰样品中所含核酸的方法，所述方法包括在酸性多糖和/或核苷酸存在下使样品与核酸改性剂接触的步骤; 以及选择性地检测来自样品中含有的活细胞的核酸的方法，所述方法包括以下步骤：（a）根据包含在所述样品中的核酸的方法修饰样品中的核酸的步骤样品，其包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 和（b）在步骤（a）之后从样品中选择性地检测未修饰的核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

2. 发明授权

US08367328B2 Method for synthesizing DNA 有权
标题翻译： DNA合成方法
公开(公告)号：US08367328B2
公开(公告)日：2013-02-05
申请号：US12285866
申请日：2008-10-15
申请人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
发明人： Kiyozo Asada , Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Osamu Takeda , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12P19/34 , C12N9/1252 , C12N9/96 , C12Q1/6806 , Y10S435/975 , C12Q2521/107
摘要： A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
摘要翻译：一种DNA合成反应增强剂，其包含选自酸性物质和阳离子配合物中的至少一种; DNA合成方法，其中在DNA合成反应中，通过使用DNA聚合酶在上述增强剂的存在下进行反应; 包含上述增强剂的DNA合成反应组合物; 包含两种以上各自具有3'→5'核酸外切酶活性的DNA聚合酶的DNA合成反应组合物; 一种DNA合成方法，其中在DNA合成反应中使用两种或更多种具有3'→5'核酸外切酶活性的DNA聚合酶; 用于体外DNA合成的试剂盒，其包含两种或更多种各自具有3'→5'核酸外切酶活性的DNA聚合酶; 以及用于体外DNA合成的试剂盒，其中试剂盒包含DNA合成反应增强剂和DNA聚合酶。根据本发明，与常规DNA合成反应相比，可以以更优异的效果进行DNA合成。

3. 发明申请

US20120270309A1 THERMAL CYCLER 审中-公开
标题翻译：热循环
公开(公告)号：US20120270309A1
公开(公告)日：2012-10-25
申请号：US13389479
申请日：2010-08-18
申请人： Tateki Arai , Osamu Takeda , Hiroyuki Izu , Hiroyuki Mukai
发明人： Tateki Arai , Osamu Takeda , Hiroyuki Izu , Hiroyuki Mukai
IPC分类号： C12M1/00
CPC分类号： B01L7/52 , B01L3/50825 , B01L2200/147 , B01L2300/1827
摘要： A thermal cycler for incubating reaction mixture, the thermal cycler including:(1) a heat block for holding and heating reaction containers which contain the reaction mixture, the heat block being a double-layered structure of a lower layer heat block and an upper layer heat block; and (2) a thermal control means for controlling a temperature of the lower layer heat block and a temperature of the upper layer heat block independently and respectively and keeping the temperature of the upper layer heat block higher than the temperature of the lower layer heat block while incubating the reaction mixture. Since the thermal cycler prevents the occurrence of the condensation of water or other components of the reaction mixture in the reaction container and the thermal differences based on the installation positions of the reaction containers, the PCR method and the other enzyme reaction can be performed in a good repeatability.
摘要翻译：一种用于温育反应混合物的热循环仪，所述热循环仪包括：（1）用于保持和加热含有反应混合物的反应容器的热块，所述加热块是下层加热块和上层的双层结构热块; 和（2）分别独立地分别控制下层热块的温度和上层热块的温度的热控制装置，并且保持上层热块的温度高于下层热块的温度同时孵育反应混合物。由于热循环仪防止反应容器中反应混合物的水或其它组分的冷凝发生，以及基于反应容器的安装位置的热差异，PCR法和其它酶反应可以在良好的重复性。

4. 发明授权

US07846703B2 Method for enhancing polymerase activity 有权
标题翻译：增强聚合酶活性的方法
公开(公告)号：US07846703B2
公开(公告)日：2010-12-07
申请号：US11866148
申请日：2007-10-02
申请人： Eiji Kobayashi , Yuki Ueda , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Eiji Kobayashi , Yuki Ueda , Yoshimi Sato , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12N9/10 , C12N9/96 , C07K14/00 , C12Q1/66 , C12P19/34
CPC分类号： C12N9/1241
摘要： A polymerase activity is effectively enhanced by adding an anionic surfactant, in particular an anionic surfactant having a polyethoxyl group, to a reaction mixture containing a polymerase.
摘要翻译：通过向含有聚合酶的反应混合物中加入阴离子表面活性剂，特别是具有聚乙氧基的阴离子表面活性剂，可有效提高聚合酶活性。

5. 发明申请

US20090047705A1 MICROORGANISM-DERIVED PSYCHROPHILIC ENDONUCLEASE 失效
标题翻译：微生物衍生的磷酸内切酶
公开(公告)号：US20090047705A1
公开(公告)日：2009-02-19
申请号：US11908062
申请日：2006-03-08
申请人： Naoyuki Awazu , Toshihiro Shodai , Hikaru Takakura , Masanari Kitagawa , Hiroyuki Mukai , Ikunoshin Kato
发明人： Naoyuki Awazu , Toshihiro Shodai , Hikaru Takakura , Masanari Kitagawa , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12P21/06 , C12N9/14 , C07H21/04 , C12P19/34 , C12N1/21
CPC分类号： C12N9/22 , C12N1/08 , C12P19/34 , C12P21/00 , C12Q1/6848
摘要： A polypeptide having an endonuclease activity derived from a psychrophilic microorganism Shewanella sp. strain AC10, which exhibits high activity at low temperatures, can remove any nucleic acid present in a protein solution and can reduce the viscosity of a protein extract; and a nucleic acid encoding the polypeptide.
摘要翻译：具有衍生自嗜冷微生物Shewanella sp。的内切核酸酶活性的多肽。在低温下表现出高活性的菌株AC10可以去除蛋白质溶液中存在的任何核酸，并且可以降低蛋白质提取物的粘度; 和编码该多肽的核酸。

6. 发明申请

US20080206811A1 Process For Producing Polypeptide 审中-公开
标题翻译：生产多肽的方法
公开(公告)号：US20080206811A1
公开(公告)日：2008-08-28
申请号：US11597318
申请日：2005-08-19
申请人： Toshihiro Shodai , Hiroshi Kobori , Takehiro Sagara , Hiroshi Endo , Hikaru Takakura , Jun Tomono , Hiroaki Sagawa , Hiroyuki Mukai , Ikunoshin Kato
发明人： Toshihiro Shodai , Hiroshi Kobori , Takehiro Sagara , Hiroshi Endo , Hikaru Takakura , Jun Tomono , Hiroaki Sagawa , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12P21/04 , C12N15/00
CPC分类号： C12N15/70 , C12N9/1276 , C12N9/22 , C12P21/02
摘要： A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.
摘要翻译：一种在低温下生产靶蛋白的方法，包括不仅引入其中引入了编码靶蛋白的基因的载体，还诱导其中引入伴侣伴侣基因的载体。

7. 发明申请

US20070298415A1 Method of Amplifying Nucleic Acid 审中-公开
标题翻译：核酸扩增方法
公开(公告)号：US20070298415A1
公开(公告)日：2007-12-27
申请号：US10582345
申请日：2004-12-06
申请人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12P19/34 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2531/119 , C12Q2525/185 , C12Q2537/143 , C12Q2525/161
摘要： In the case where a template nucleic acid has sequences substantially the same as each other, a primer is designed in a region between these sequences so as to give a product in a ladder structure in which a target region is polymerized in a single sequence via the same sequences in the ICAN reaction. By using a chimeric oligonucleotide primer and a ladder-forming oligonucleotide primer containing a specific base sequence, an amplification product having a ladder structure can be positively formed and thus the sensitivity, amplification efficiency and reaction speed in the ICAN reaction can be elevated.
摘要翻译：在模板核酸具有彼此基本相同的序列的情况下，在这些序列之间的区域中设计引物，以便产生梯形结构中的产物，其中靶区域通过经由在ICAN反应中相同的序列。通过使用嵌合寡核苷酸引物和含有特定碱基序列的梯形寡核苷酸引物，可以积极地形成具有梯形结构的扩增产物，从而可以提高ICAN反应中的灵敏度，扩增效率和反应速度。

8. 发明授权

US06949634B2 Method for synthesizing cDNA 失效
公开(公告)号：US06949634B2
公开(公告)日：2005-09-27
申请号：US10201314
申请日：2002-07-24
申请人： Junko Yamamoto , Kazue Miyake , Hiroyuki Mukai , Fumitsugu Hino , Ikunoshin Kato
发明人： Junko Yamamoto , Kazue Miyake , Hiroyuki Mukai , Fumitsugu Hino , Ikunoshin Kato
IPC分类号： C12N15/10 , C12Q1/68 , C07H19/00 , C12N9/00 , C12P19/34
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/686 , C12Q2521/319 , C12Q2521/107
摘要： A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.

9. 发明申请

US20050118578A1 Amplified nucleic acids and immobilized products thereof 审中-公开
标题翻译：扩增核酸及其固定化产物
公开(公告)号：US20050118578A1
公开(公告)日：2005-06-02
申请号：US10467325
申请日：2002-02-06
申请人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
发明人： Junichi Mineno , Osamu Takeda , Masatomo Rokushima , Takashi Uemori , Shigekazu Hokazono , Hiroyuki Mukai , Shusaku Yamashita , Hiroaki Sagawa , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6837 , C12Q2565/518 , C12Q2531/119
摘要： A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.
摘要翻译：通过使用嵌合寡核苷酸引物通过核酸扩增反应大量提供的核酸以含有修饰的脱氧核糖核苷酸的状态构建，用于将核酸固定至固相，然后固定于固体相，从而得到具有优异品质的固定化核酸产物。

10. 发明授权

US06218150B1 DNA polymerase-related factors 有权
公开(公告)号：US06218150B1
公开(公告)日：2001-04-17
申请号：US09446504
申请日：1999-12-23
申请人： Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Tomoko Fujita , Kazue Miyake , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12P1934
CPC分类号： C12N9/1252
摘要： The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.

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