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41. 发明公开

EP1748068A4 METHOD OF BREEDING LIPID-PRODUCING STRAIN AND UTILIZATION OF THE SAME 有权
标题翻译： VERFAHREN ZURZÜCHTUNGEINES LIPIDPRODUZIERENDEN STAMMS UND NUTZUNG DAVON
公开(公告)号：EP1748068A4
公开(公告)日：2007-08-08
申请号：EP05727395
申请日：2005-03-28
申请人： SUNTORY LTD
发明人： OCHIAI MISA , KAWASHIMA HIROSHI , SHIMIZU SAKAYU , SAKURADANI EIJI
IPC分类号： C12N15/09 , C12N1/00 , C12N1/15 , C12N15/88 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6472 , C12P7/6463 , C12R1/645
摘要： A method of breeding a lipid-producing strain belonging to the genus Mortierella. According to the method of breeding a lipid-producing strain which involves an expression-inhibiting step of inhibiting the expression of a specific gene in the lipid-producing strain as described above, a lipid-producing strain belonging to the genus Mortierella can be efficiently and effectively bred.
摘要翻译：一种育种属于被孢霉属的脂质生产菌株的方法。根据如上所述的涉及抑制脂质生产菌株中的特定基因表达的表达抑制步骤的脂质生产菌株的育种方法，属于被孢霉属的脂质生产菌株可以被有效地有效繁殖。

42. 发明公开

EP0978563A4 DELTA 9-DESATURASE GENE 失效
标题翻译： DELTA-9 DESATURASE GEN
公开(公告)号：EP0978563A4
公开(公告)日：2002-04-10
申请号：EP98905692
申请日：1998-02-27
申请人： SUNTORY LTD
发明人： SHIMIZU SAKAYU , KOBAYASHI MICHIHIKO
IPC分类号： C12N9/02 , C12P7/64 , C12N15/52 , C12N1/15 , C12N15/63 , C12N15/80
CPC分类号： C12Y106/02002 , C12N9/0083 , C12P7/6427 , C12P7/6472 , C12Y114/19001
摘要： Genome DNA and cDNA encoding Δ9-desaturase derived from a microorganism belonging to Mortierella s.p. mortierella, an expression vector and a transformant for expressing the same, and a process for preparing Δ9-desaturase using a gene encoding the enzyme. Introduction of the Δ9-desaturase gene into cells capable of producing an unsaturated fatty acid can enhance the conversion to palmitoleic acid and oleic acid as starting compounds of unsaturated fatty acids, resulting in improved productivity of the unsaturated fatty acids. When a cytochrome b5 gene or a cytochrome b5 reductase gene, i.e., a constituent of a microsomal electron transport system, is combined with the Δ9-desaturase, more efficient production can be expected. Further, the Δ9-desaturase can be efficiently produced by a recombinant DNA method.
摘要翻译：公开了来自属于被孢霉属的被孢霉属亚属的微生物的表达载体和转化体的编码DELTA 9-去饱和酶的基因组DNA和cDNA。还公开了通过使用编码该酶的基因产生DELTA 9-去饱和酶的方法。将本发明的DELTA 9-去饱和酶基因引入不饱和脂肪酸生产细胞可以增加转化为棕榈油酸或油酸，不饱和脂肪酸的起始原料，并且可以增加不饱和脂肪酸的产生。通过将细胞色素b5的基因或细胞色素b5还原酶的基因，微粒体电子传递系统的组分与本发明的DELTA 9-去饱和酶组合，可以预期更有效的生产。也可以通过重组DNA技术以高效率生产DELTA 9-去饱和酶。

43. 发明公开

EP0957173A4 PROCESS FOR PREPARING FAT OR OIL CONTAINING UNSATURATED FATTY ACID 失效
标题翻译：用于生产不饱和脂肪酸的含酸性脂肪或油
公开(公告)号：EP0957173A4
公开(公告)日：2000-02-02
申请号：EP97937813
申请日：1997-08-27
申请人： SUNTORY LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU
IPC分类号： A23C9/152 , A23C11/06 , A23D9/00 , A23D9/007 , A23K1/16 , A23L1/29 , A23L1/30 , A61K31/19 , C12M1/04 , C12N1/14 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： A process for preparing fat or oil containing unsaturated fatty acids, characterized by cultivating a microorganism belonging to the subgenus Mortierella or the genus Mortierella in a medium containing a nitrogen source derived from a soybean and harvesting the fat or oil containing unsaturated fatty acids from the culture. The process can provide fat or oil having a low 24,25-methylenecholest-5-en-3β-o1 content.
摘要翻译：其通过培养属于被孢霉属亚属被孢霉在含有源自大豆氮源的培养基中的微生物，并从培养产物中收集含不饱和脂肪酸的油，其特征为生产不饱和含脂肪酸的油的方法，全部。这些油得到低24,25- methylenecholest -5-烯-3β醇含量。

44. 发明专利

DK0957173T3 未知
公开(公告)号：DK0957173T3
公开(公告)日：2017-07-17
申请号：DK97937813
申请日：1997-08-27
申请人： SUNTORY LTD
发明人： AKIMOTO KENGO , HIGASHIYAMA KENICHI , SHIMIZU SAKAYU
IPC分类号： A23C9/152 , A23C11/06 , A23D9/00 , A23D9/007 , A23L33/00 , A23L33/11 , A23L33/115 , A23L33/135 , A61K31/19 , C12M1/04 , C12N1/14 , C12P7/64 , C12R1/645
摘要： A process for production of unsaturated fatty acid-containing oils which is characterized by culturing a microorganism belonging to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean, and collecting the unsaturated fatty acid-containing oil from the cultured product. The oils are obtained with a low 24,25-methylenecholest-5-en-3 beta -ol content.

45. 发明专利

DE69738377T2 未知
公开(公告)号：DE69738377T2
公开(公告)日：2008-12-04
申请号：DE69738377
申请日：1997-10-09
申请人： SUNTORY LTD , NIPPON SUISAN KAISHA LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU , DOISAKI NOBUSHIGE , FURIHATA KIYOMI
IPC分类号： A23C9/152 , A23D9/007 , A23D9/00 , A23L1/30 , C12P7/64 , C12R1/645
摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula. Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 ² -ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpina of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.

46. 发明专利

DE69738377D1 未知
公开(公告)号：DE69738377D1
公开(公告)日：2008-01-24
申请号：DE69738377
申请日：1997-10-09
申请人： SUNTORY LTD , NIPPON SUISAN KAISHA LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU , DOISAKI NOBUSHIGE , FURIHATA KIYOMI
IPC分类号： A23C9/152 , A23D9/007 , A23D9/00 , A23L1/30 , C12P7/64 , C12R1/645
摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula. Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 ² -ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpina of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.

47. 发明专利

CA2276179C MEDIA FOR CULTURING MICROORGANISMS AND PROCESS FOR PRODUCINGUNSATURATED FATTY ACIDS OR LIPIDS CONTAINING THE SAME 未知
公开(公告)号：CA2276179C
公开(公告)日：2008-01-08
申请号：CA2276179
申请日：1997-12-26
申请人： SUNTORY LTD
发明人： SHIMIZU SAKAYU , AKIMOTO KENGO , YAGUCHI TOSHIAKI , HIGASHIYAMA KENICHI
IPC分类号： C12N1/14 , C12P7/64
摘要： A process for controlling the mycelial morphology of a microorganism belonging to the genus Mortierella during culturing and a process for producing unsaturated fatty acids and a lipid containing them by using a culture medium for culturing a microorganism in which phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the culture medium are in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively, characterized in that the microorganism belonging to the genus Mortierella is cultured in a culture medium containing phosphate ions in the range of 5 to 60 mM, potassium ions in the range of 5 to 60 mM, sodium ions in the range of 2 to 50 mM, magnesium ions in the range of 0.5 to 9 mM, and calcium ions in the range of 0.5 to 12 mM, respectively, to produce unsaturated fatty acids and the lipid containing them, and the culture medium for culturing a microorganism having phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the range of S to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively.

48. 发明专利

AT380472T 未知
公开(公告)号：AT380472T
公开(公告)日：2007-12-15
申请号：AT97943165
申请日：1997-10-09
申请人： SUNTORY LTD , NIPPON SUISAN KAISHA LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU , DOISAKI NOBUSHIGE , FURIHATA KIYOMI
IPC分类号： A23C9/152 , A23D9/007 , A23D9/00 , A23L1/30 , C12P7/64 , C12R1/645
摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula. Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 ² -ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpina of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.

49. 发明专利

DE69735278T2 未知
公开(公告)号：DE69735278T2
公开(公告)日：2006-08-03
申请号：DE69735278
申请日：1997-08-27
申请人： SUNTORY LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU
IPC分类号： C12P7/64 , A23C9/152 , A23C9/20 , A23C11/06 , A23D9/00 , A23D9/007 , A23L33/00 , A23L33/11 , A23L33/115 , A23L33/135 , A61K31/19 , C12M1/04 , C12N1/14 , C12R1/645
摘要： A process for production of unsaturated fatty acid-containing oils which is characterized by culturing a microorganism belonging to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean, and collecting the unsaturated fatty acid-containing oil from the cultured product. The oils are obtained with a low 24,25-methylenecholest-5-en-3 beta -ol content.

50. 发明专利

PT825263E 未知
公开(公告)号：PT825263E
公开(公告)日：2004-07-30
申请号：PT97306494
申请日：1997-08-26
申请人： SUNTORY LTD
发明人： AKIMOTO KENGO , KAWASHIMA HIROSHI , SHIMIZU SAKAYU
IPC分类号： C12P7/64 , C12R1/645
摘要： The present invention discloses a process for producing lipid containing omega-9 highly unsaturated fatty acid by culturing in a medium a mutant strain obtained by mutation on a microorganism having the ability to produce arachidonic acid belonging to the genus Mortierella and so forth, in which DELTA 12 desaturation activity is decreased or lost, but at least one of DELTA 5 desaturation activity, DELTA 6 desaturation activity and chain length elongation activity is elevated. Moreover, the present invention also discloses a process for producing omega-9 highly unsaturated fatty acid by collecting omega-9 highly unsaturated fatty acid from the culture or lipid described above.

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