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1. 发明申请

WO2004033698A3 PROCESS FOR PRODUCING MICROBIAL FAT OR OIL HAVING LOWERED UNSAPONIFIABLE MATTER CONTENT AND SAID FAT OR OIL 审中-公开
标题翻译：用于生产具有降低的不可皂化物质含量并说明脂肪或油的微生物脂肪或油的方法
公开(公告)号：WO2004033698A3
公开(公告)日：2004-05-27
申请号：PCT/JP0313000
申请日：2003-10-09
申请人： SUNTORY LTD , AKIMOTO KENGO , HIGASHIYAMA KENICHI , KAWASHIMA HIROSHI , SUMIDA MOTOO
发明人： AKIMOTO KENGO , HIGASHIYAMA KENICHI , KAWASHIMA HIROSHI , SUMIDA MOTOO
IPC分类号： A23D9/02 , C12P7/64 , A23D9/007
CPC分类号： C12P7/6472 , A23D9/02
摘要： A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (= d) to the inner diameter of the culture tank (= D) is d/D = 0.30 to 0.6.
摘要翻译：一种生产具有降低的不皂化物含量和/或酯型甾醇含量并且包含高度不饱和脂肪酸作为构成脂肪酸的原油的方法，其特征在于能够生产包含不饱和脂肪酸作为构成脂肪酸的培养基在装备有搅拌叶轮的培养槽内在含有2-15％氮源浓度的培养基中培养，搅拌叶轮满足搅拌叶轮直径（= d）与内径培养槽（= D）为d / D = 0.30〜0.6。

2. 发明申请

WO2006085672A3 PRODUCTION OF POLYUNSATURATED FATTY ACIDS USING NOVEL CELL TREATMENT METHOD 审中-公开
标题翻译：使用新型细胞处理方法生产聚氨酯脂肪酸
公开(公告)号：WO2006085672A3
公开(公告)日：2007-04-26
申请号：PCT/JP2006302589
申请日：2006-02-08
申请人： SUNTORY LTD , HIGASHIYAMA KENICHI , NAKAJIMA TOSHIHARU
发明人： HIGASHIYAMA KENICHI , NAKAJIMA TOSHIHARU
IPC分类号： A23K1/00 , A23L1/00 , A61K31/00 , C11B1/00 , C12N1/00 , C12N1/04 , C12N1/12 , C12N1/14 , C12P1/00 , C12P1/02 , C12P7/64
CPC分类号： A23D9/00 , A23K10/12 , A23K20/158 , A23L33/115 , A23V2002/00 , C11B1/10 , C12N1/005 , C12P7/6472 , C12P7/6481 , A23V2250/1882
摘要： A method for isolating one compound or more than one compound from a biomass which contains microorganisms that have produced the compound or compounds, the method comprising the following steps: (a) preparing or obtaining wet cells having an average moisture content of between 30% and 80%; (b) subjecting the wet cells to primary drying to obtain primary dried cells having an average moisture content of between 5% and 50%; (c) subjecting the primary dried cells obtained in (b) to secondary drying to obtain secondary dried cells having an average moisture content of no greater than 10%; and (d) extracting or isolating, purifying and/or refining the compound or each of the compounds from the secondary dried cells obtained in (c) .
摘要翻译：一种从含有生成化合物或微生物的微生物的生物质中分离一种化合物或一种以上化合物的方法，该方法包括以下步骤：（a）制备或获得平均含水量在30％至 80％; （b）使湿细胞进行一次干燥，得到平均含水量在5％至50％之间的初级干燥细胞; （c）使（b）中获得的一次干燥的细胞二次干燥，得到平均含水量不大于10％的二次干燥细胞; 和（d）从（c）中获得的二次干燥细胞提取或分离，纯化和/或精制化合物或每种化合物。

3. 发明申请

WO2004024930A3 PROCESS FOR PRODUCTION OF TRANSESTERIFIED OILS/FATS OR TRIGLYCERIDES 审中-公开
标题翻译：生产经过转化的油/ FATS或TRIGLYCERIDES的方法
公开(公告)号：WO2004024930A3
公开(公告)日：2004-07-08
申请号：PCT/JP0311744
申请日：2003-09-12
申请人： SUNTORY LTD , SUMIDA MOTOO , HIGASHIYAMA KENICHI
发明人： SUMIDA MOTOO , HIGASHIYAMA KENICHI
IPC分类号： A23D9/00 , A23K1/16 , A23L1/30 , C11C3/10 , C12P7/64 , A23C11/02 , A23D9/007 , A23D9/02 , A23K1/00 , A23L1/29
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/115 , A23V2002/00 , C11C3/10 , A23V2250/1862
摘要： There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides with vegetable oils/fats or triglycerides using 1,3-position specific type lipases.
摘要翻译：提供了含有不饱和脂肪酸的油/脂肪或甘油三酸酯，具有高消化吸收性能和抗氧化损伤性，适用于婴幼儿改良牛奶，食品和健康食品和/或补品等领域。通过使用1,3位特异性脂肪酶将多不饱和脂肪酸的油/脂肪或甘油三酯与植物油/脂肪或甘油三酸酯进行酯交换。

4. 发明专利

JP2004129504A Method for production of astaxanthin-containing lipid 审中-公开
标题翻译：用于生产含有ASTAXANTHIN的脂质的方法
公开(公告)号：JP2004129504A
公开(公告)日：2004-04-30
申请号：JP2002294420
申请日：2002-10-08
申请人： Suntory Ltd , サントリー株式会社
发明人： HIGASHIYAMA KENICHI , NISHIO NAOMICHI , KAKIZONO SHIYUNEI
IPC分类号： C12P7/64 , C12N1/12 , C12P23/00
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently producing an astaxanthin-containing lipid with which a method for culturing a green alga Haematococcus pluvialis is improved and a cyst is thereby formed and matured under dark conditions.
SOLUTION: A method for producing an algal body of the green alga Haematococcus pluvialis in which astaxanthin is accumulated in an amount of ≥40-700 pg/cell in the cyst obtained by culturing the green alga Haematococcus pluvialis under conditions of 0.01-0.5 vvm relative volume of ventilation in the dark. Furthermore, there is provided the method for producing the astaxanthin-containing lipid consisting essentially of a component selected from the group consisting of free type astaxanthin, an astaxanthin fatty acid monoester, an astaxanthin fatty acid diester and a mixture thereof. The method is characterized by affording the astaxanthin-containing substance from the algal body.
COPYRIGHT: (C)2004,JPO
摘要翻译：解决问题的方案：提供一种高效生产含有青海洋黄素的脂质的方法，通过该方法培养绿藻类红潮球菌的方法，由此在黑暗条件下形成囊泡并成熟。解决方案：一种生产绿藻类红潮球菌的藻体的方法，其中在通过在0.01-0.1％的条件下培养绿藻红球藻获得的囊肿中，虾青素以≥40-700pg/ 0.5vvm相对体积的通风在黑暗中。此外，提供了生产含有虾青素的脂质的方法，其基本上由选自游离型虾青素，虾青素脂肪酸单酯，虾青素脂肪酸二酯及其混合物的组分组成。该方法的特征在于从藻体中提供含有虾青素的物质。版权所有（C）2004，JPO

5. 发明专利

JP2000069987A PRODUCTION OF ARACHIDONIC ACID-CONTAINING LIPID AND DIHOMO-GAMMA-LINOLENIC ACID-CONTAINING LIPID 失效
公开(公告)号：JP2000069987A
公开(公告)日：2000-03-07
申请号：JP24358398
申请日：1998-08-28
申请人： SUNTORY LTD
发明人： AKIMOTO KENGO , HIGASHIYAMA KENICHI , SHIMIZU AKIRA
IPC分类号： C12P7/64 , C12R1/645
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing an arachidonic acid or dihomo-γ-linolenic acid-containing lipid having an increased ratio of arachidonic acid or dihomo-γ-linolenic acid to the whole fatty acids. SOLUTION: A variant reduced or deficient in ω3 desaturation activity obtained by varying a fungus belonging to the genus Mortierella, etc., capable of producing arachidonic acid is cultured in a medium at a temperature lower than the optimal growth temperature from the beginning of the culture or after the culturing at the optimal growth temperature. A lipid containing arachidonic acid is collected from the culture product.

6. 发明专利

JP2008113671A Edible oil containing arachidonic acid originating from microorganism, food additive composed of the same, and food containing the same and method for producing this edible oil containing arachidonic acid 有权
标题翻译：含有微生物的食用油的食用油，与其组合的食品添加剂和含有该食品的食品以及生产含有阿拉西酸的食用油的方法
公开(公告)号：JP2008113671A
公开(公告)日：2008-05-22
申请号：JP2008024423
申请日：2008-02-04
申请人： Nippon Suisan Kaisha Ltd , Suntory Ltd , サントリー株式会社 , 日本水産株式会社
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU AKIRA , DOISAKI SHINJI , FURUHATA KIYOMI
IPC分类号： A23C9/152 , A23D9/007 , A23D9/00 , A23D9/02 , A23L1/30 , C12P7/64 , C12R1/645
CPC分类号： A23D9/00 , A23L33/12 , A23L33/40 , A23V2002/00 , A23V2250/1862
摘要： PROBLEM TO BE SOLVED: To provide an edible oil containing arachidonic acid originating from microorganism belonging to Mortierella subgenus of Mortierella which has arachidonic acid productivity, with a reduced amount of unsaponifiable matter, scarcely containing sterol which has cyclopropane ring and is inexperienced in food, suitable for food, especially for infant's milk preparation. SOLUTION: The food additive comprises less than 0.8 wt.%, preferably less than 0.6 wt.% of unsaponifiable matter and the oil containing arachidonic acid originating from microorganism containing more than 20 wt.% of the arachidonic acid. The prescribed oil additive contains less than 0.3 wt.%, preferably less than 0.15 wt.% of 24, 25-methylenecholest-5-en-3β-ol. The microorganism belongs to the Mortierella subgenus of Mortierella which has arachidonic acid productivity. The microorganism belonging to the Mortierella subgenus is a microorganism belonging to genus Mortierella alpina species. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供一种含有花生四烯酸的食用油，其来源于具有花生四烯酸生产力的被孢霉属的被孢霉属亚种的微生物，具有减少量的不皂化物质，几乎不含有具有环丙烷环并且没有经验的甾醇食物，适合食物，特别适合婴幼儿的奶制品。
解决方案：食品添加剂包含小于0.8重量％，优选小于0.6重量％的不皂化物质和来自含有超过20重量％花生四烯酸的微生物的含花生四烯酸的油。规定的油添加剂含有小于0.3重量％，优选小于0.15重量％的24,25-亚甲基胆甾-5-烯-3β-醇。该微生物属于具有花生四烯酸生产力的被孢霉属的被孢霉属亚属。属于被孢霉属亚属的微生物是属于被孢霉属的被孢霉属的微生物。版权所有（C）2008，JPO＆INPIT

7. 发明专利

JPH11243981A PRODUCTION OF OIL AND FAT CONTAINING ARACHIDONIC ACID AND/OR EICOSAPENTAENOIC ACID 失效
公开(公告)号：JPH11243981A
公开(公告)日：1999-09-14
申请号：JP5202198
申请日：1998-03-04
申请人： SUNTORY LTD
发明人： AKIMOTO KENGO , HIGASHIYAMA KENICHI , SHIMIZU AKIRA
IPC分类号： C12P7/64
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an oil and fat having elevated contents of arachidonic acid and/or eisosapentaenoic acid. SOLUTION: A microorganism having a productivity of an ω 9-based highly unsaturated fatty acid, having a Δ5 unsaturation enzyme activity and a Δ6 unsaturation enzyme activity, deteriorated or deleted from a Δ12 unsaturation enzyme activity and having the elevated activity for producing the Δ5 unsaturation enzyme activity and/or Δ6 unsaturation enzyme activity by a mutation treatment is cultured in a culture medium containing specific unsaturated fatty acids added thereto.

8. 发明专利

JPH1070992A PRODUCTION OF OIL AND FAT CONTAINING UNSATURATED FATTY ACID 失效
公开(公告)号：JPH1070992A
公开(公告)日：1998-03-17
申请号：JP23021096
申请日：1996-08-30
申请人： SUNTORY LTD
发明人： HIGASHIYAMA KENICHI , AKIMOTO KENGO , SHIMIZU SAKAYU
IPC分类号： A23C9/152 , A23C11/06 , A23D9/00 , A23D9/007 , A23L33/00 , A23L33/11 , A23L33/115 , A23L33/135 , A61K31/19 , C12M1/04 , C12N1/14 , C12P7/64 , C12R1/645 , A23K1/16 , A23L1/30
摘要： PROBLEM TO BE SOLVED: To obtain the subject safe oil and fat containing little amount of specific impurities by culturing microorganisms belonging to the subgenus Mortierella in the genus Mortierella in a medium containing nitrogen sources obtained from soybeans and extracting the produced oil and fat from the culture product. SOLUTION: The subject oil and fat containing unsaturated fatty acids, e.g. γ-linolenic acid, dihomo-γ-linolenic acid, arachidonic acid, eicosapentaenoic acid, etc., a low amount such as

9. 发明专利

JP2006219528A Manufacturing method of highly unsaturated fatty acid using new cell body-treating method 有权
标题翻译：使用新的细胞体内处理方法制备高度不饱和脂肪酸的方法
公开(公告)号：JP2006219528A
公开(公告)日：2006-08-24
申请号：JP2005031918
申请日：2005-02-08
申请人： Suntory Ltd , サントリー株式会社
发明人： HIGASHIYAMA KENICHI , NAKAJIMA TOSHIHARU
IPC分类号： C11B1/10 , A23D9/00 , A23J7/00 , A23K1/16 , A23K1/18 , A23L1/30 , C05F11/00 , C11B11/00 , C11C3/00 , C12N1/14 , C12N1/16 , C12P7/64 , C12R1/645 , C12R1/785
CPC分类号： A23D9/00 , A23K10/12 , A23K20/158 , A23L33/115 , A23V2002/00 , C11B1/10 , C12N1/005 , C12P7/6472 , C12P7/6481 , A23V2250/1882
摘要： PROBLEM TO BE SOLVED: To provide a new isolation method of biomass, that is, a new drying method by a combination of a plurality of methods, and a manufacturing method and a preservation method of PUFA (polyunsaturated fatty acid)-containing oils and fats (triglycerides) and/or a PUFA-containing phospholipid, or a PUFA-containing cell body comprising cooling and subsequently filling and packaging a dried cell body. SOLUTION: The method is one for isolating one or more kinds of compounds from a biomass-containing microorganism having produced such compounds and comprises the following steps; (a) a step for preparing or obtaining a wet cell body having an average water content of 30-80%; (b) a step for feeding the wet cell body to a primary drier followed by molding to obtain a molded cell body having an average water content of 5-50%; (c) a step for subjecting the molded cell body obtained in the step (b) to a secondary drying to obtain a dried cell body having an average water content of at most 10%; and (d) a step for purifying, extracting or isolating the compound or the compounds from the dried cell body obtained in the step (c). COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供生物质的新的分离方法，即通过多种方法的组合的新的干燥方法以及含有PUFA（多不饱和脂肪酸）的制造方法和保存方法油和脂肪（甘油三酸酯）和/或含PUFA的磷脂或含PUFA的细胞体，其包括冷却并随后填充和包装干细胞体。解决方案：该方法是用于从生产这种化合物的含生物质的微生物中分离一种或多种化合物的方法，包括以下步骤：（a）制备或获得平均含水量为30-80％的湿细胞体的步骤; （b）将湿细胞体供给到初级干燥器中，随后成型以获得平均含水量为5-50％的模制电池体的步骤; （c）使步骤（b）中获得的模制电池体进行二次干燥以获得平均含水量为至多10％的干燥电池体的步骤; 和（d）从步骤（c）获得的干燥细胞体中纯化，提取或分离化合物或化合物的步骤。版权所有（C）2006，JPO＆NCIPI

10. 发明专利

JP2006067964A Method for culturing fungal cell body 有权
标题翻译：培养真菌细胞体的方法
公开(公告)号：JP2006067964A
公开(公告)日：2006-03-16
申请号：JP2004258151
申请日：2004-09-06
申请人： Suntory Ltd , サントリー株式会社
发明人： HIGASHIYAMA KENICHI
IPC分类号： C12N1/14 , C12P7/64
CPC分类号： C12P7/6472 , C12N1/14 , C12P7/6427
摘要： PROBLEM TO BE SOLVED: To provide a method for culturing fungal cell bodies, capable of keeping good productivity of a highly unsaturated fatty acid or a compound containing the highly unsaturated fatty acid as a constituent fatty acid, without requiring to actually seek an oxygen-transfer coefficient (KLa) when scaling-up thereof is conducted. SOLUTION: This method for culturing the fungal cell bodies comprises using a culturing apparatus of aeration and agitation type, wherein agitation power and a maximum aeration amount of the culturing apparatus are adjustable and controllable, so that mechanical agitation of the apparatus is conducted by keeping the agitation power per unit amount of a liquid at 269 (W/m 3 ) or less, until a prescribed time has elapsed since culturing started, and then the maximum aeration amount and a maximum agitation power requirement of the apparatus are increased to ranges satisfying: the KLa expressed by (P/V) 0.95 /Vs 0.67 [P is an agitation power requirement (W); V is an amount of the liquid (m 3 ); and Vs is an aeration linear velocity (m/sec)] is not less than 59; an aeration linear velocity parameter expressed by Vs 0.67 [(m/sec) 0.67 ] is not less than 0.075; and an agitation power requirement parameter expressed by (P/V) 0.95 [(W/m 3 ) 0.95 ] is not less than 203, after the prescribed time has elapsed. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种培养真菌细胞体的方法，其能够保持高度不饱和脂肪酸或含有高度不饱和脂肪酸的化合物作为构成脂肪酸的生产率，而不需要实际寻求氧气传递系数（KLa）在进行放大时。解决方案：用于培养真菌细胞体的方法包括使用通气和搅拌类型的培养装置，其中培养装置的搅拌功率和最大通气量是可调节和可控的，从而进行设备的机械搅拌通过将每单位量的液体的搅拌功率保持在269（W / m 3 / SP>）以下，直到从培养开始经过规定时间，然后将最大通气量和最大搅拌功率将装置的要求提高到满足以下范围：由（P / V） 0.95表示的KLa / Vs 0.67 [P是搅拌功率要求（W） V是液体的量（m 3 ）; 而Vs是曝气线速度（m / sec）]不小于59; 由Vs 0.67表示的曝气线速度参数[（m / sec） 0.67 ]不小于0.075; 并且由（P / V） 0.95 [（W / m 3 ） 0.95 表示的搅拌功率要求参数不小于203，经过规定的时间。版权所有（C）2006，JPO＆NCIPI

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