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1. 发明专利

CA2282860C .DELTA.9-DESATURASE GENE 未知
公开(公告)号：CA2282860C
公开(公告)日：2009-05-05
申请号：CA2282860
申请日：1998-02-27
申请人： SUNTORY LTD
发明人： KOBAYASHI MICHIHIKO , SHIMIZU SAKAYU
IPC分类号： C12N15/53 , C12N1/15 , C12N9/02 , C12N15/80 , C12P7/64
摘要： Genomic DNA and cDNA encoding .DELTA.9-desaturase from a microorganism belonging to the subgenus Mortierella of the genus Mortierella, an expression vector for expression thereof, and a transformant are disclosed. A method for producing .DELTA.9-desaturase by use of a gene encoding the enzyme is also disclosed. Introduction of the .DELTA.9-desaturase gene of the present invention into an unsaturated fatty acid producing cell can enhance conversion into palmitoleic acid or oleic acid, starting materials for unsaturated fatty acids, and can increase the production of unsaturated fatty acids. By combining a gene for cytochrome b5 or a gene for cytochrome b5 reductase, constituents of the microsomal electron transport system, with the .DELTA.9-desaturase of the present invention, more efficient production can be expected. .DELTA.9-desaturase can also be produced with high efficiency by a recombinant DNA technology.

2. 发明专利

CA2282860A1 .DELTA.9-DESATURASE GENE 未知
公开(公告)号：CA2282860A1
公开(公告)日：1998-09-03
申请号：CA2282860
申请日：1998-02-27
申请人： SUNTORY LTD
发明人： SHIMIZU SAKAYU , KOBAYASHI MICHIHIKO
IPC分类号： C12N9/02 , C12P7/64 , C12N15/52 , C12N1/15 , C12N15/63 , C12N15/80
摘要： Genome DNA and cDNA encoding .DELTA.9-desaturase derived from a microorganism belonging to Mortierella s.p. mortierella, an expression vector and a transformant for expressing the same, and a process for preparing .DELTA.9desaturase using a gene encoding the enzyme. Introduction of the .DELTA.9desaturase gene into cells capable of producing an unsaturated fatty acid can enhance the conversion to palmitoleic acid and oleic acid as starting compounds of unsaturated fatty acids, resulting in improved productivity of the unsaturated fatty acids. When a cytochrome b5 gene or a cytochrome b5 reductase gene, i.e., a constituent of a microsomal electron transport system, is combined with the .DELTA.9-desaturase, more efficient production can be expected. Further, the .DELTA.9-desaturase can be efficiently produced by a recombinant DNA method.

3. 发明公开

EP0978563A4 DELTA 9-DESATURASE GENE 失效
标题翻译： DELTA-9 DESATURASE GEN
公开(公告)号：EP0978563A4
公开(公告)日：2002-04-10
申请号：EP98905692
申请日：1998-02-27
申请人： SUNTORY LTD
发明人： SHIMIZU SAKAYU , KOBAYASHI MICHIHIKO
IPC分类号： C12N9/02 , C12P7/64 , C12N15/52 , C12N1/15 , C12N15/63 , C12N15/80
CPC分类号： C12Y106/02002 , C12N9/0083 , C12P7/6427 , C12P7/6472 , C12Y114/19001
摘要： Genome DNA and cDNA encoding Δ9-desaturase derived from a microorganism belonging to Mortierella s.p. mortierella, an expression vector and a transformant for expressing the same, and a process for preparing Δ9-desaturase using a gene encoding the enzyme. Introduction of the Δ9-desaturase gene into cells capable of producing an unsaturated fatty acid can enhance the conversion to palmitoleic acid and oleic acid as starting compounds of unsaturated fatty acids, resulting in improved productivity of the unsaturated fatty acids. When a cytochrome b5 gene or a cytochrome b5 reductase gene, i.e., a constituent of a microsomal electron transport system, is combined with the Δ9-desaturase, more efficient production can be expected. Further, the Δ9-desaturase can be efficiently produced by a recombinant DNA method.
摘要翻译：公开了来自属于被孢霉属的被孢霉属亚属的微生物的表达载体和转化体的编码DELTA 9-去饱和酶的基因组DNA和cDNA。还公开了通过使用编码该酶的基因产生DELTA 9-去饱和酶的方法。将本发明的DELTA 9-去饱和酶基因引入不饱和脂肪酸生产细胞可以增加转化为棕榈油酸或油酸，不饱和脂肪酸的起始原料，并且可以增加不饱和脂肪酸的产生。通过将细胞色素b5的基因或细胞色素b5还原酶的基因，微粒体电子传递系统的组分与本发明的DELTA 9-去饱和酶组合，可以预期更有效的生产。也可以通过重组DNA技术以高效率生产DELTA 9-去饱和酶。

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