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    • 31. 发明专利
    • Electrochemical distinguishment of cells
    • 细胞电化学鉴定
    • JPS59179098A
    • 1984-10-11
    • JP5277383
    • 1983-03-30
    • Tadashi Matsunaga
    • MATSUNAGA TADASHI
    • C12Q1/04
    • PURPOSE: A couple of electrodes are inserted into a cell suspension to measure differential currents to effect clear distinguishment and identification between individual cells.
      CONSTITUTION: A suspension of cells of, e.g., fungi or actinomycetes, erythrocytes, leukocytes, tumor cells or cultivated animal or plant cells is poured into a cell for differential pulse polarography which is composed of the cell 4 equipped with the action electrode 1, the opposite electrode 2 and the reference electrode of saturated sodium chloride calomel electrode 3, pulse sequencer 5, potential programmer 6, potentiostat 7, drop knocker 8, i/E converter 9, sample holds (τ, τ') 10, 11, difference assembly 12 and recorder 13. An increasingly scanning or sweeping potential resulting from accumulation of micropotentials is applied to between the electrodes to measure the occurring current and obtain a current- potential curve which gives clear peak potentials differing according to the kinds of cells.
      COPYRIGHT: (C)1984,JPO&Japio
    • 目的:将一对电极插入细胞悬浮液中以测量差分电流,以实现各个细胞之间的明确区分和识别。 构成:将例如真菌或放线菌,红细胞,白细胞,肿瘤细胞或培养的动物或植物细胞的细胞的悬浮液倒入用于差动脉冲极谱法的细胞中,所述细胞由装备有动作电极1的细胞4, 相对电极2和饱和氯化钠甘汞电极3的参比电极,脉冲序列发生器5,电位编程器6,恒电位仪7,降幅环8,I / E转换器9,样品保持(τ,τ')10,11,差分组件 12和记录器13.由电极积累产生的越来越多的扫描或扫描电位施加到电极之间以测量发生的电流并获得电流 - 电位曲线,其给出根据电池种类不同的清晰的峰值电位。
    • 32. 发明专利
    • Electrochemical measurement of cell activity
    • 细胞活性的电化学测量
    • JPS59179097A
    • 1984-10-11
    • JP5277283
    • 1983-03-30
    • Tadashi Matsunaga
    • MATSUNAGA TADASHI
    • C12Q1/04C12Q1/06C12R1/01C12R1/125C12R1/19C12R1/865G01N27/416
    • PURPOSE: A couple of electrodes 4, 4' are inserted into a cell suspension and the electric current is measured in the presence of 4,4'-bipyridine to enable high- accuracy measurement of the electrochemical activity of the cell in a short time.
      CONSTITUTION: A suspension of living cells of, e.g., a variety of microorganisms such as actinomycetes or fungi, erythrocytes, leukocytes, tumor cells, cultivated animal or plant cells and 4,4'-bipyridine are poured into an electrolytic cell 4 and the action electrode 1, the opposite electrode 2 and the reference electrode 3 such as SSCE are inserted into the cell. Periodically scanning or sweeping potential is applied from the power source 6 through the potentiostat 5 to between these electrodes are the occurring current is recorded as a currrent- potential curve in the X-Y recorder or synchroscope 7 to determine clear peak potentials differing according to the kinds of cells or peak current values proportional to cell concentration.
      COPYRIGHT: (C)1984,JPO&Japio
    • 目的:将一对电极4,4'插入电池悬浮液中,并在4,4'-联吡啶存在下测量电流,以便在短时间内高精度地测量电池的电化学活性。 构成:将诸如放线菌或真菌,红细胞,白细胞,肿瘤细胞,培养的动物或植物细胞和4,4'-联吡啶等各种微生物的活细胞悬浮液倒入电解槽4中, 电极1,相对电极2和诸如SSCE的参比电极3插入电池中。 定时扫描或扫描电位从电源6通过恒电位器5施加到这些电极之间,所发生的电流被记录为XY记录器或同步器7中的电流 - 电位曲线,以确定根据不同种类的不同的峰值电位 细胞或峰值电流值与细胞浓度成比例。
    • 35. 发明专利
    • Electrochemical water control method and apparatus
    • 电化学水控制方法和装置
    • JP2005131611A
    • 2005-05-26
    • JP2003373592
    • 2003-10-31
    • Tadashi MatsunagaPentel Corpぺんてる株式会社是 松永
    • TAKAYANAGI HIROICHIKADOI HIDEOOZAWA KINICHITAKIMOTO TOSHIHIROTAKAHASHI HIROMICHIWAKE HITOSHIMATSUNAGA TADASHI
    • E02B1/00C02F1/46C23F15/00E03C1/266
    • PROBLEM TO BE SOLVED: To provide a method and apparatus for electrochemically preventing organisms, scales, or the like from being deposited on an offshore structure, ship, piping or waterway for water transportation, drain port, water storage tank, heat exchanger, or screen of sea water intake or the like by controlling the amount of microorganism and organic matter in the water, and for electrochemically controlling water by controlling the amount of microorganism and organic matter contained in the water.
      SOLUTION: The method and apparatus for electrochemically controlling water comprises at least two or more conductive base materials, a counter electrode, and a power source installed at the counter electrode so as to be energizable. In the method and apparatus, a voltage is applied to at least one or more non-elution type conductive base material by the power source to cause an electrochemical reaction on the surface of the non-elution type conductive base material, and/or a voltage is applied to the one or more elution type conductive base material by the power source to elute the elution type conductive base material, thereby controlling the amount of microorganism and organic matter in the water.
      COPYRIGHT: (C)2005,JPO&NCIPI
    • 要解决的问题:提供一种用于电化学防止生物,鳞片等沉积在用于水运,排水口,储水箱,热交换器的海上结构,船舶,管道或水道上的方法和装置 ,或者通过控制水中的微生物和有机物质的量,以及通过控制水中含有的微生物和有机物的量来电化学地控制水来进行海水摄取等的筛选。 解决方案:用于电化学控制水的方法和装置包括至少两个或更多个导电基材,对电极和安装在对电极上的电源以便被激励。 在该方法和装置中,通过电源将至少一种或多种非洗脱型导电性基材施加电压,使非洗脱型导电性基材的表面发生电化学反应,和/或电压 通过电源施加到一种或多种洗脱型导电性基材上,洗脱洗脱型导电性基材,从而控制水中的微生物和有机物的量。 版权所有(C)2005,JPO&NCIPI
    • 37. 发明专利
    • Microorganism separating device
    • 微生物分离装置
    • JP2007089566A
    • 2007-04-12
    • JP2006195978
    • 2006-07-18
    • Tokyo Univ Of Agriculture & TechnologyToshiba Corp国立大学法人東京農工大学株式会社東芝
    • MATSUNAGA TADASHITAKEYAMA HARUKOTAGUCHI TOMOYUKIHARAGUCHI SATOSHIYOSHINO MASATOISHIMORI YOSHIOKANEKO MASAOTOYODA HIROKAZU
    • C12M1/26
    • C12M47/02
    • PROBLEM TO BE SOLVED: To provide a microorganism separating device capable of selectively separating a target microorganism from sample water with good accuracy and recovering the microorganism.
      SOLUTION: This microorganism separating device is equipped with a microchip 20 for capturing the target microorganism contained in the sample water, a sample water supply means 19 for supplying the sample water to the microchip 20, and a sample water suction means 21 for sucking the sample water from the microchip 20. The microchip 20 has a flat plate substrate 10 on which a flow channel 11 having a fluid supply port 13, a fluid suction port 15, and a fluid discharge port 14 are formed and a capturing part 12 positioned on the fluid suction port 15 of the flat plate substrate 10. The capturing part 12 of the microchips has a plurality of open holes 12a each having a diameter smaller than a minimum diameter of the target microorganism.
      COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供能够以良好的精度选择性地将目标微生物与样品水分离并回收微生物的微生物分离装置。 解决方案:该微生物分离装置配备有用于捕获试样水中所含的目标微生物的微芯片20,将样品水供给到微芯片20的试样供水装置19,以及用于 从微芯片20吸取样本水。微芯片20具有平板基板10,在其上形成有具有流体供给口13,流体吸入口15和流体排出口14的流路11,并且捕获部12 定位在平板基板10的流体吸入口15上。微芯片的捕获部分12具有多个直径小于目标微生物的最小直径的开孔12a。 版权所有(C)2007,JPO&INPIT