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31. 发明专利

JPH0276568A LASER PERFORATION UNIT FOR RAW CELL 失效
公开(公告)号：JPH0276568A
公开(公告)日：1990-03-15
申请号：JP22871888
申请日：1988-09-14
申请人： HITACHI LTD
发明人： MURAKAMI SEI , HOSOMI NOBUYUKI , TAKAI MASAO
IPC分类号： G02B21/18 , C12M1/00
摘要： PURPOSE:To provide the title unit designed so that a polarizing plate with its direction of polarization rectangular to that for the laser beams going toward an ocular through reflection is located in front of the ocular, thereby protecting the eyes of an operator from the laser beams. CONSTITUTION:Laser beams emitted from a source 1 is passed through a polarizing plate 2 into a unidirectional polarized laser beams, which are then collected, through an objective lens 6, onto a sample 4 to perforate raw cells. The image of the sample 4 illuminated by an illumination lamp 5 and the laser beams reflected by the sample 4 and the objective lens 6 enter together a polarizing plate 3 located in front of an ocular 7. In this case, an illuminating light from the lamp 5 can be passed through the polarizing plate 3 because of being unpolarized, but the laser beams are polarized by the polarizing plate 2; hence, if the direction of polarization for the plate 3 is made to be rectangular to that for the laser beams, said laser beams cannot be passed through the polarizing plate 3, and the image of the sample 4 passed through the polarizing plate 3 will enter the eyes of an operator through the ocular 7 and can be observed; thus protecting the eyes of the operator from the laser beams.

32. 发明专利

JPS62205779A LASER PROCESSING DEVICE 失效
公开(公告)号：JPS62205779A
公开(公告)日：1987-09-10
申请号：JP4616286
申请日：1986-03-05
申请人： HITACHI LTD
发明人： KANEKIYO HIROSHI , HOSOMI NOBUYUKI , MURAKAMI SEI
IPC分类号： C12N15/09 , C12M1/00 , C12N15/00
摘要： PURPOSE:To prevent danger of laser beam reaching an ocular part, etc., and to process a fine, raw test specimen safely while observing, by irradiating laser beam scanned by a scanning part having an angle of rotation of a scanning mirror restricted to a specific range. CONSTITUTION:A scanning mirror 3 is constructed in such a way that an allowable angle of rotation is restricted in order to make irradiation of laser beam downward by a restricting fitment 7 for angle of rotation and the fitment 7 is attached to a scanner 4. Then, laser beam is introduced to a scanning mirror 1 to adjust an angle of rotation and reflected by another scanner 2, introduced to the scanning mirror 3 and reflected so that a raw test specimen such as animal, vegetable cell, microorganism, etc., on a test specimen stand 5 is irradiated with the laser beam. On the other hand, observing lighting is sent the test specimen from the lower part of the test specimen stand 5 by illumination 6 and the raw test specimen is processed by the laser beam while observing it by an ocular part 8.

33. 发明专利

JPS62192290A LASER BEAM MACHINE 失效
公开(公告)号：JPS62192290A
公开(公告)日：1987-08-22
申请号：JP3298486
申请日：1986-02-19
申请人： HITACHI LTD , RIKAGAKU KENKYUSHO
发明人： HOSOMI NOBUYUKI , KANEKIYO HIROSHI , MURAKAMI SEI , KASUYA KEIKO , TSUKAGOSHI MIKIRO
IPC分类号： C12N15/09 , B23K26/382 , C12M1/00 , C12N1/00 , C12N5/00 , C12N5/10 , C12N13/00 , C12N15/00
摘要： PURPOSE:To perform the laser beam machining properly for various samples by projecting the laser light from one direction of a sample by the 1st optical path, by projecting on the sample from another direction by the 2nd optical path by providing an optical path switching means. CONSTITUTION:The sample case 20 containing a sample is set on a sample base 2, the image picked up by a TV camera 25 is displayed on a monitor TV 40 and a table driving part 3 is roughly set to the position suitable for the work by driving the table driving part 3 with watching this display. The alignment of the position where the laser light of each sample is projected and the laser light projecting position is then performed. Shutters 52, 53 are closed at the completion time of this alignment. The laser light emitted from a laser light source (YAG laser) 1 selects the shutters 52, 53 of an optical path shifting device 5, thereafter, the work is executed by projecting the laser light on the sample through the selected optical path by the shutter 7 or 8, or shutters 7 and 8.

34. 发明专利

JPS62191749A CARRIER FREE ELECTROPHORETIC APPARATUS 失效
公开(公告)号：JPS62191749A
公开(公告)日：1987-08-22
申请号：JP3302486
申请日：1986-02-19
申请人： HITACHI LTD
发明人： MUROI KATSUMI , ODA CHIKAO , YOSHINAGA SHOJI , HOSOMI NOBUYUKI
IPC分类号： G01N27/447 , B01D57/02 , G01N27/26
摘要： PURPOSE:To enhance the separation capacity of a charge substance by eliminating the disturbance of the flow of the liquid in a migration chamber, by adhering a heat dissipating sheet having high heat conductivity, a good electric insulating property and liquid leakage preventing effect to the membrane isolating an electrode chamber. CONSTITUTION:A migration chamber 3(3a, 3b) and an electrode chamber 2 are isolated by a semipermeable membrane 4 and the migration chambers 3a, 3b are separated by a filter membrane 5. A supply port 11 for a solution of a charge substance to be separated, an upper take-out port 12 for the liquid after separation and a lower take out port 13 are provided to the migration chamber 3. Each of the semipermeable membrane 14 and the filter membrane 15 is held between heat dissipating sheets 14 each comprising a membrane like member having a large number of perforations. Each heat dissipating sheet 14 is formed using a material having high heat conductivity, a good electric insulating property and liquid leakage preventing effect, for example, a composition prepared by mixing a fiber or powder of Al2O3, AlN, C or glass with a gasket material such as silicone rubber. By this method, the removal of Joule heat generated at the time of electrophoresis and the deformation of the membranes due to electroosmosis are prevented.

35. 发明专利

JPS62161490A WORKING METHOD FOR RAW SAMPLE 失效
公开(公告)号：JPS62161490A
公开(公告)日：1987-07-17
申请号：JP58286
申请日：1986-01-08
申请人： HITACHI LTD
发明人： KAWAMOTO MUNEO , HOSOMI NOBUYUKI , KANEKIYO HIROSHI , MURAKAMI SEI , YOSHINAGA SHOJI , KIMURA NOBUO
IPC分类号： C12N1/00 , B23K26/02 , B23K26/04 , C12N5/00 , C12Q1/02
摘要： PURPOSE:To execute the working correctly for the scheduled position of a raw sample by projecting the ray for working by correcting it in case of the projection at the succeeding time by the slipping portion between the projection position obtd. from an image after projection of the ray for working and the calculated position to be worked. CONSTITUTION:A TV camera 9 picks up the image of a raw sample via a microscopic device 10, outputs an image signal, digitizes said signal by A/D 21 and stores it in an image memory 22. A monitor TV 24 displays this contents. An operator indicates the work position of the raw sample displayed on the screen with a light pen 25 with watching a TV 24 and this indicating position is operated inside an arithmetic processing part 23. And in the processing part 23, the contents of the memory 22 is taken in and the position of a spot light is found with its image processing. In this way the processing part 23 outputs a spot position control signal to a spot position control device 7 so as to overlap spot light on said indication position by light pen.

36. 发明专利

JPS62158480A METHOD FOR PROCESSING RAW TEST SPECIMEN 失效
公开(公告)号：JPS62158480A
公开(公告)日：1987-07-14
申请号：JP58186
申请日：1986-01-08
申请人： HITACHI LTD
发明人： KAWAMOTO MUNEO , HOSOMI NOBUYUKI , KANEKIYO HIROSHI , MURAKAMI SEI , YOSHINAGA SHOJI , KIMURA NOBUO
IPC分类号： C12N15/09 , C12M1/00 , C12M1/42 , C12N1/00 , C12N5/00 , C12N13/00 , C12N15/00
摘要： PURPOSE:To prevent various germs from invading a zone of the aimed raw test specimen, by irradiating the surroudings of the raw test specimen to be processed with sterilizing light rays and preventing invasion of various germs. CONSTITUTION:At a calculation treatment part 14 control actions to carry out processing of raw test specimen is done depending upon content of an image memory 12 and indication position detected, by a light pen 13'. At the calculation treatment part 14, a zone except a zone wherein the raw test specimen exist in a container, namely the surroundings of existing zone of the raw test specimen is subjected to control wherein surroundings are irradiated with ultraviolet rays (sterilizing light rays) generated from an ultraviolet light source 30 and various germs are destroyed. Consequently, the various germs can be prevented from invading the existing zone of the aimed test specimen.

37. 发明专利

JPS61224982A METHOD OF CONTROLLING PH OF CULTURE TANK 失效
公开(公告)号：JPS61224982A
公开(公告)日：1986-10-06
申请号：JP6353285
申请日：1985-03-29
申请人： HITACHI LTD
发明人： MURAKAMI SEI , HOSOMI NOBUYUKI , KANEKIYO HIROSHI
IPC分类号： C12M1/36 , C12N1/00
摘要： PURPOSE:To shorten intervals of pH control, and to carry out pH control of culture tank in high accuracy, by measuring time lag from the time of feeding pH adjustor to the time when pH substantially changes, and regulating the intervals of feed of pH adjuster depending upon the time lag. CONSTITUTION:The pH control device 5 calculates an amount of the pH adjuster 1 required to eliminate the difference between a set pH value and measured pH value by the pH meter 4 depending upon the difference. Then, the number of opening times of the solenoid valve 2 corresponding to the amount of the pH adjuster to be added is obtained, and the valve 2 is continuously opened and closed the number of opening times. Consequently, an amount of the pH adjuster 1 corresponding to the number o the opening times is added. During the operation, the above-mentioned pH control operation in the device 5 is carried out intermittently, and intervals of feed of the pH adjuster 1 is set by calculating time lag from the time of feeding the pH adjuster 1 to the time when pH changes substantially in the culture tank 3, and taking the time lag into consideration.

38. 发明专利

JPS61205477A CULTURE APPARATUS 失效
公开(公告)号：JPS61205477A
公开(公告)日：1986-09-11
申请号：JP4651285
申请日：1985-03-11
申请人： HITACHI LTD
发明人： HOSOMI NOBUYUKI , KANEKIYO HIROSHI , MURAKAMI SEI
IPC分类号： C12M1/36 , C12M1/00
摘要： PURPOSE:To provide a culture apparatus having excellent operability and safety, by attaching a culture tank at the lower front part of a casing, attaching a storage tank of conditioning solution to the right or left side of the upper front part of the casing and attaching the operation devices to the part opposite to the storage tank. CONSTITUTION:The culture tank 11 is attached to the lower front part of the casing, and the storage tanks 12-14 are placed to the upper front part of the casing at the right side of the center line A. The operation boards 15, 16 are arranged to the upper front part of the casing at the left side of the center line A. The operation devices can be shielded from the heat of the flame generated in the supply of conditioning liquid to the storage tanks 12-14 by this arrangement.

39. 发明专利

JPS61202681A BEARING-LESS CULTURE APPARATUS 失效
公开(公告)号：JPS61202681A
公开(公告)日：1986-09-08
申请号：JP4262385
申请日：1985-03-06
申请人： HITACHI LTD
发明人： KANEKIYO HIROSHI , HOSOMI NOBUYUKI , MURAKAMI SEI
IPC分类号： C12M1/02
摘要： PURPOSE:To enable the elimination of adverse effects to culture liquid such as the disintegration of cells with the bearing in the tank, contamination of the culture liquid caused by the abrasion of the bearing, etc., by rotating a stirring blade with a magnetic supporting member without using a bearing in the tank. CONSTITUTION:A cylindrical partition wall 3 is protruded from the bottom into the main body 1 of the culture tank, and a rotary shaft 4 is inserted into the cylindrical wall 3. Plural magnets 11 are fixed to the rotary shaft 4 with definite intervals along the direction of the shaft. A stirring blade 15 is fixed to the outer circumference of the partition wall 3 in the tank 1, and the magnet supporting member 13 having fixed magnet 14 is inserted in a freely rotatable manner. The magnet 11 fixed to the rotary shaft 4 and the magnet 14 fixed to the magnet supporting member 13 are disposed in a manner to generate repulsive force with each other and to effect the rotation of the stirring blade 15. The bearing in the tank is eliminated by this method, and accordingly, the adverse effects to culture liquid such as the disintegration of cells with the bearing in the tank, contamination of the culture liquid caused by the abra sion of the bearing, etc., can be eliminated.

40. 发明专利

JPS6122159A Method of operating helium liquefying refrigerator 失效
标题翻译：操作液体冷冻机的方法
公开(公告)号：JPS6122159A
公开(公告)日：1986-01-30
申请号：JP14231084
申请日：1984-07-11
申请人： Hitachi Ltd
发明人： HOSOMI NOBUYUKI , MATSUMOTO KOUZOU
IPC分类号： F25B9/02 , C07C213/10

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