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31. 发明授权

US07287055B2 Web-based groupware system 有权
公开(公告)号：US07287055B2
公开(公告)日：2007-10-23
申请号：US10898131
申请日：2004-07-22
申请人： Michael J. Cannata , Richard Lee , Matthew Powell , Ryan Ramasra , Gerald William Smith , Charles Edward Tatham , Randall Nelson Remme
发明人： Michael J. Cannata , Richard Lee , Matthew Powell , Ryan Ramasra , Gerald William Smith , Charles Edward Tatham , Randall Nelson Remme
IPC分类号： G06F15/16
CPC分类号： G06Q10/10 , H04L29/06 , H04L67/02 , H04L67/2819 , H04L67/2842 , H04L67/36 , H04L67/42 , H04L69/329
摘要： The present invention relates to a system and method for providing a communication network. The system comprises a ‘network-connected server having input and access capabilities, a site builder, a transmitter, a communicator, and memory. The site-builder receives instructions input from a first user and creates a dedicated network site based on the received instructions. The transmitter communicates the existence of the dedicated network site to a nominated second user. The communicator provides accesses to the contents of the dedicated network site by the first and second users. The memory stores information input by the first and the second user in the dedicated network site.

32. 发明申请

US20070243636A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070243636A1
公开(公告)日：2007-10-18
申请号：US11692754
申请日：2007-03-28
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/543
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

33. 发明申请

US20070166766A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070166766A1
公开(公告)日：2007-07-19
申请号：US11692776
申请日：2007-03-28
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

34. 发明申请

US20060270062A1 MASS SPECTROMETRIC IMMUNOASSAY 审中-公开
公开(公告)号：US20060270062A1
公开(公告)日：2006-11-30
申请号：US11462504
申请日：2006-08-04
申请人： Randall Nelson , Peter Williams , Jennifer Krone
发明人： Randall Nelson , Peter Williams , Jennifer Krone
IPC分类号： G01N33/543 , G01N33/00
CPC分类号： G01N24/00 , C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , G01N33/6851 , Y10T436/24
摘要： Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.

35. 发明申请

US20060127948A1 ANALYSIS OF PROTEINS FROM BIOLOGICAL FLUIDS USING MASS SPECTROMETRIC IMMUNOASSAY 审中-公开
标题翻译：使用大量光谱免疫测定从生物流体中分析蛋白质
公开(公告)号：US20060127948A1
公开(公告)日：2006-06-15
申请号：US11163588
申请日：2005-10-24
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53 , G01N33/00
CPC分类号： G01N33/6848 , G01N33/6851 , Y10T436/24
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states.
摘要翻译：本文提供的是存在于复杂生物流体或提取物中的蛋白质的质谱法免疫测定（MSIA）的方法，装置和试剂盒。含有多孔固体载体的移液器尖端，其用亲和配体共价衍生并用于从各种生物流体提取特异性蛋白质及其变体。使用一系列缓冲液和水冲洗将非特异性结合的化合物从萃取装置中冲洗，之后将野生型蛋白（和/或其变体）直接洗脱到靶上用于分析，例如基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）。洗脱的样品的质谱随后通过精确的分子量测定鉴定的保留的蛋白质。蛋白质和变体水平可以使用定量方法来确定，其中蛋白质/变体信号被归一化为内部参考标准物质的信号（在MSIA分析前掺入样品或与靶蛋白共提取的其它内源性蛋白质）并且该值与由含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较。这样的MSIA设备，套件和方法在以下领域有重要的应用：基础研究和开发，蛋白质组学，蛋白质结构表征，药物发现，药物靶标发现，治疗监测，临床监测和诊断，以及大量人群的高通量筛选，以建立和识别蛋白质/变异体模式，区分健康与病态。

36. 发明申请

US20050153311A1 Mass spectrometric immunoassay analysis of specific proteins and variants present in various biological fluids 失效
标题翻译：对各种生物液体中存在的特定蛋白质和变体的质谱免疫分析
公开(公告)号：US20050153311A1
公开(公告)日：2005-07-14
申请号：US10958867
申请日：2004-10-04
申请人： Randall Nelson , Peter Williams , Jennifer Krone
发明人： Randall Nelson , Peter Williams , Jennifer Krone
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/543
CPC分类号： C12Q1/6816 , C12Q1/6872 , G01N33/5302 , G01N33/54373 , Y10T436/24
摘要： Presented herein is the construction of pipettor tips (termed MSIA-Tips) containing porous solid supports that are constructed, covalently derivatized with affinity ligand, and used to extract specific proteins and their variants from various biological fluids by repeatedly flowing the fluids through the MSIA-Tips. A second protein species (a mass-shifted variant of the targeted protein doped into the samples at a constant concentration) is co-extracted with the endogenous protein and variants and is used as a quantitative internal reference standard (IRS). Nonspecifcally bound compounds are rinsed from the MSIA-Tip using a series of buffer and water rinses, after which the wild type protein, protein variants and the IRS are eluted from the MSIA-Tips directly onto a target in preparation for analysis such as MALDI-TOF. Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels are determined via a quantitative method in which the protein/variant signals are normalized to the signal of the IRS and the values compared to a working curve constructed from samples containing known concentrations of the protein or variants.
摘要翻译：这里提出的是构建含有多孔固体支持物的移液器尖端（称为MSIA-Tips），其构建为用亲和配体共价衍生，并且用于通过重复地使流体流过MSIA-DNA来从各种生物流体中提取特异性蛋白质及其变体，提示。将第二种蛋白质物种（以恒定浓度掺入样品中的靶向蛋白质的质量转移变体）与内源蛋白质和变体共萃取并用作定量内部参照标准品（IRS）。使用一系列缓冲液和水冲洗从MSIA-Tip中冲洗非特异性结合的化合物，之后将野生型蛋白质，蛋白质变体和IRS直接从MSIA-Tip中洗脱到靶上用于分析，如MALDI- TOF。洗脱的样品的质谱随后通过精确的分子量测定鉴定的保留的蛋白质。通过定量方法测定蛋白质和变体水平，其中将蛋白质/变体信号归一化为IRS的信号，并且该值与由含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较。

37. 发明申请

US20050003373A1 AN INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES 有权
标题翻译：用于分析生物分子的综合高通量系统
公开(公告)号：US20050003373A1
公开(公告)日：2005-01-06
申请号：US10710991
申请日：2004-08-16
申请人： Kemmons Tubbs , Karl Gruber , Randall Nelson
发明人： Kemmons Tubbs , Karl Gruber , Randall Nelson
IPC分类号： B01D15/08 , B01J20/281 , B01J20/32 , C12M1/34 , C12Q1/68 , G01N30/02 , G01N33/68 , G01N33/558
CPC分类号： G01N33/6845 , B01J20/28097 , B01J20/281 , B01J20/286 , B01J20/32 , B01J20/3204 , B01J20/3208 , B01J20/3242 , B01J20/3259 , B01J20/3265 , B01J20/3272 , B01J20/3274 , B01J20/3282 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/48 , G01N30/6095 , G01N33/54366 , G01N33/545 , G01N33/6803 , G01N2570/00 , H01J49/164 , H01J49/40 , Y10T436/255 , B01D15/3804
摘要： Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
摘要翻译：描述了一种亲和力微柱，其包含高表面积材料，其具有包含在壳体内的高流动性和低死体积，并且具有结合到高表面积材料的表面的亲和试剂，其被激活或可激活。结合亲和微柱表面的亲和试剂还包含用于整合生物分子的高通量分析的亲和受体。

38. 发明申请

US20050003372A1 AN INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES 有权
公开(公告)号：US20050003372A1
公开(公告)日：2005-01-06
申请号：US10710990
申请日：2004-08-16
申请人： Kemmons Tubbs , Karl Gruber , Randall Nelson
发明人： Kemmons Tubbs , Karl Gruber , Randall Nelson
IPC分类号： B01D15/08 , B01J20/281 , B01J20/32 , C12M1/34 , C12Q1/68 , G01N30/02 , G01N33/68 , G01N33/558
CPC分类号： G01N33/6845 , B01J20/28097 , B01J20/281 , B01J20/286 , B01J20/32 , B01J20/3204 , B01J20/3208 , B01J20/3242 , B01J20/3259 , B01J20/3265 , B01J20/3272 , B01J20/3274 , B01J20/3282 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/48 , G01N30/6095 , G01N33/54366 , G01N33/545 , G01N33/6803 , G01N2570/00 , H01J49/164 , H01J49/40 , Y10T436/255 , B01D15/3804
摘要： Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

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