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1. 发明申请

US20070243635A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070243635A1
公开(公告)日：2007-10-18
申请号：US11692713
申请日：2007-03-28
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/543
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

2. 发明申请

US20070128734A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
标题翻译：特异性生物流体蛋白质质谱免疫分析方法与装置
公开(公告)号：US20070128734A1
公开(公告)日：2007-06-07
申请号：US11671576
申请日：2007-02-06
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/543
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.
摘要翻译：本文介绍的是存在于复杂生物液体或提取物中的蛋白质及其变体的质谱法免疫测定（MSIA）的方法，装置和试剂盒。蛋白质和变体水平可以使用定量方法来确定，其中蛋白质/变体信号被归一化为内部参考标准物质的信号（在MSIA分析前掺入样品或与靶蛋白共提取的其它内源性蛋白质）并且与从含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较的值。

3. 发明申请

US20070259447A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070259447A1
公开(公告)日：2007-11-08
申请号：US11694180
申请日：2007-03-30
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/566
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

4. 发明申请

US20070166767A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070166767A1
公开(公告)日：2007-07-19
申请号：US11694143
申请日：2007-03-30
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53 , G06F19/00
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

5. 发明申请

US20070128663A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070128663A1
公开(公告)日：2007-06-07
申请号：US11671603
申请日：2007-02-06
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

6. 发明申请

US20060121533A1 ANALYSIS OF PROTEINS FROM BIOLOGICAL FLUIDS USING MASS SPECTROMETRIC IMMUNOASSAY 审中-公开
公开(公告)号：US20060121533A1
公开(公告)日：2006-06-08
申请号：US11163587
申请日：2005-10-24
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53 , G01N33/00
CPC分类号： G01N33/6848 , G01N33/6851 , Y10T436/24
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states.

7. 发明申请

US20050164409A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
标题翻译：特异性生物流体蛋白质质谱免疫分析方法与装置
公开(公告)号：US20050164409A1
公开(公告)日：2005-07-28
申请号：US10905029
申请日：2004-12-10
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/00 , G01N33/543 , G01N33/68
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.
摘要翻译：本文介绍的是存在于复杂生物液体或提取物中的蛋白质及其变体的质谱法免疫测定（MSIA）的方法，装置和试剂盒。蛋白质和变体水平可以使用定量方法测定，其中蛋白质/变体信号被归一化为内部参考标准物质的信号（在MSIA分析前掺入样品或与靶蛋白共提取的其它内源性蛋白质）并且与从包含已知浓度的蛋白质或变体的样品构建的工作曲线相比较的值。

8. 发明申请

US20070243636A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070243636A1
公开(公告)日：2007-10-18
申请号：US11692754
申请日：2007-03-28
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/543
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

9. 发明申请

US20070166766A1 METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
公开(公告)号：US20070166766A1
公开(公告)日：2007-07-19
申请号：US11692776
申请日：2007-03-28
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53
CPC分类号： G01N33/6803
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

10. 发明申请

US20060127948A1 ANALYSIS OF PROTEINS FROM BIOLOGICAL FLUIDS USING MASS SPECTROMETRIC IMMUNOASSAY 审中-公开
标题翻译：使用大量光谱免疫测定从生物流体中分析蛋白质
公开(公告)号：US20060127948A1
公开(公告)日：2006-06-15
申请号：US11163588
申请日：2005-10-24
申请人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
发明人： Urban Kiernan , Eric Niederkofler , Kemmons Tubbs , Dobrin Nedelkov , Randall Nelson
IPC分类号： G01N33/53 , G01N33/00
CPC分类号： G01N33/6848 , G01N33/6851 , Y10T436/24
摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to a working curves constructed from samples containing known concentrations of the protein or variants. Such MSIA devices, kits and methods have significant application in the fields of; basic research and development, proteomics, protein structural characterization, drug discovery, drug-target discovery, therapeutic monitoring, clinical monitoring and diagnostics, as well as in the high throughput screening of large populations to establish and recognize protein/variant patterns that are able to differentiate healthy from diseased states.
摘要翻译：本文提供的是存在于复杂生物流体或提取物中的蛋白质的质谱法免疫测定（MSIA）的方法，装置和试剂盒。含有多孔固体载体的移液器尖端，其用亲和配体共价衍生并用于从各种生物流体提取特异性蛋白质及其变体。使用一系列缓冲液和水冲洗将非特异性结合的化合物从萃取装置中冲洗，之后将野生型蛋白（和/或其变体）直接洗脱到靶上用于分析，例如基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）。洗脱的样品的质谱随后通过精确的分子量测定鉴定的保留的蛋白质。蛋白质和变体水平可以使用定量方法来确定，其中蛋白质/变体信号被归一化为内部参考标准物质的信号（在MSIA分析前掺入样品或与靶蛋白共提取的其它内源性蛋白质）并且该值与由含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较。这样的MSIA设备，套件和方法在以下领域有重要的应用：基础研究和开发，蛋白质组学，蛋白质结构表征，药物发现，药物靶标发现，治疗监测，临床监测和诊断，以及大量人群的高通量筛选，以建立和识别蛋白质/变异体模式，区分健康与病态。

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