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21. 发明授权

US08859221B2 Method for measuring high density lipoprotein cholesterol 有权
标题翻译：高密度脂蛋白胆固醇测定方法
公开(公告)号：US08859221B2
公开(公告)日：2014-10-14
申请号：US11798141
申请日：2007-05-10
申请人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
发明人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
IPC分类号： C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92
摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
摘要翻译：本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件用于HDL-C检测。本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。和来源于假单胞菌属的胆固醇氧化酶。用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。

22. 发明授权

US08168406B2 Dry analytical element for lipase measurement 有权
标题翻译：用于脂肪酶测量的干燥分析元件
公开(公告)号：US08168406B2
公开(公告)日：2012-05-01
申请号：US12410035
申请日：2009-03-24
申请人： Jun Arakawa , Nobuhito Masuda , Kentaro Nakamura , Shigeki Kageyama
发明人： Jun Arakawa , Nobuhito Masuda , Kentaro Nakamura , Shigeki Kageyama
IPC分类号： C12Q1/44 , C12M1/36 , C12M1/34 , C12M1/00
CPC分类号： C12Q1/44 , G01N2333/92
摘要： The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 μm or less.
摘要翻译：本发明提供了含有含有碳原子数12〜22的长链烷基脂肪酸甘油三酯，单甘油酯脂肪酶，甘油测定试剂的体液中所含的胰脂肪酶的测定用干燥分析元件的制造方法，水不渗透性支持体和至少一种扩散剂或试剂层，所述方法包括涂布平均粒径为1μm以下的甘油三酯的乳液/分散液的步骤。

23. 发明申请

US20080182285A1 METHOD FOR MEASURING ANIMAL alpha-AMYLASE 有权
标题翻译：用于测量动物α-淀粉酶的方法
公开(公告)号：US20080182285A1
公开(公告)日：2008-07-31
申请号：US12015151
申请日：2008-01-16
申请人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
发明人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
IPC分类号： C12Q1/40
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译：本发明的目的是提供一种能够测量没有稀释度的动物样品的高浓度（高达2500U / L）的方法，其甚至能够特异性地测量α-淀粉酶，即使在包含动物α-淀粉酶的样品中，淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。本发明提供了一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯在其还原末端，反应的pH为6以上且小于7。

24. 发明授权

US5300421A Method for quantitative measurement of trace enzyme and substrate used therein 失效
标题翻译：其中使用的痕量酶和底物的定量测定方法
公开(公告)号：US5300421A
公开(公告)日：1994-04-05
申请号：US727347
申请日：1991-07-08
申请人： Nobuhito Masuda , Nobuo Suzuki , Shoji Ishiguro , Mitsunori Ono
发明人： Nobuhito Masuda , Nobuo Suzuki , Shoji Ishiguro , Mitsunori Ono
IPC分类号： C12Q1/00 , G01N33/535 , G01N33/58 , G01N33/53
CPC分类号： C12Q1/00 , G01N33/535 , G01N33/581
摘要： A synthetic substrate used for quantitative analysis of a trace enzyme. The substrate has a molecular structure (B)-(A)-(C) comprising at least one structure (A) catalytically affected by the enzyme to be analysed, at least one photographically active labelling structure (B) linked to the structure (A) and at least one specific adsorbing structure (C) linked to the structure (A). The substrate is used in a quantitative analysis of a trace enzyme, in which the structure (A) or the linkage between the structure (A) and the structure (C), or the linkage between the structure (B) and the structure (A) is cleaved by the action of the analyte enzyme. The reaction product of the enzymatic reaction is separated by allowing the same to contact with an adsorbent for the structure (C). The separated reaction product is developed photographically and then the optical density of the resultant developed silver and/or colored dye is measured.
摘要翻译：用于痕量酶定量分析的合成底物。底物具有分子结构（B） - （A） - （C） - 包含至少一种被待分析酶催化影响的结构（A），至少一种与结构连接的照相活性标记结构（B））和至少一种与结构（A）连接的特定吸附结构（C）。该基质用于微量酶的定量分析，其中结构（A）或结构（A）和结构（C）之间的连接或结构（B）和结构（A）之间的连接）被分析物酶的作用切割。通过使酶反应的反应产物与用于结构（C）的吸附剂接触来分离。分离出的反应产物用照相机显影，然后测量所得显影的银和/或着色染料的光密度。

25. 发明授权

US4975366A Multi-layered element for quantititative analysis of immuno reactant 失效
标题翻译：用于定量分析免疫反应物的多层元件
公开(公告)号：US4975366A
公开(公告)日：1990-12-04
申请号：US15916
申请日：1987-02-18
申请人： Yukio Sudo , Nobuhito Masuda , Kenji Miura
发明人： Yukio Sudo , Nobuhito Masuda , Kenji Miura
IPC分类号： G01N31/22 , C12Q1/00 , G01N33/52 , G01N33/543
CPC分类号： G01N33/54386 , G01N33/525 , Y10S435/805 , Y10S436/81
摘要： A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.
摘要翻译：用于定量分析具有不透水和透光性支持层的免疫反应物的多层元件; 由所述载体层承载并含有亲水性聚合物作为粘合剂的着色试剂层; 以及覆盖所述试剂层并由多孔基体制成的反应层。当分析物免疫反应物是抗原时，所述反应层含有针对抗原的特异性抗体，抗体由第一水不溶性载体固定。当分析物免疫反应物是抗体时，所述反应层含有抗体的特异性抗原。反应层含有由不同于第一水不溶性载体的第二水不溶性载体固定的酶底物。着色试剂层含有检测试剂组合物，用于与通过免疫反应物的酶标记的复合物和由第二水不溶性载体固定的酶底物之间的反应产生的酶反应产物偶联。当检测试剂组合物与酶反应产物偶联时，显色，测量其光密度以确定液体样品中所含的分析物免疫反应物的量。

26. 发明授权

US4950454A Member of analytical element for the analysis of liquid sample containing solid 失效
标题翻译：用于分析含有固体的液体样品的分析元素成员
公开(公告)号：US4950454A
公开(公告)日：1990-08-21
申请号：US338992
申请日：1989-04-14
申请人： Nobuhito Masuda , Takeshi Igarashi
发明人： Nobuhito Masuda , Takeshi Igarashi
IPC分类号： G01N33/52
CPC分类号： G01N33/525 , Y10S435/805 , Y10T436/25375
摘要： A member of an analytical element for the analysis of a liquid sample containing solid material, comprising a volume filtration layer composed of a fibrous material and a spreading layer having a liquid-retaining capacity larger than that of the volume filtration layer, in which the volume filtration layer and the spreading layer are united to give an integrated structure. The spreading layer may be composed of a fibrous material, a woven cloth, a knitted cloth or a non-fibrous porous medium.

27. 发明授权

US4414323A Method for measuring trace enzyme 失效
标题翻译：痕量酶的测定方法
公开(公告)号：US4414323A
公开(公告)日：1983-11-08
申请号：US298813
申请日：1981-09-02
申请人： Nobuhito Masuda
发明人： Nobuhito Masuda
IPC分类号： G01N33/536 , B68G7/02 , C12Q1/00 , C12Q1/34 , G01N33/53 , G01N33/535 , G01N33/52 , G01N33/54 , G01N33/58
CPC分类号： G01N33/535 , C12Q1/34 , C12Q2334/00 , G01N2333/924 , G01N2333/976 , Y10S435/962 , Y10S436/805
摘要： In a photochemical measurement methodof a trace enzyme which comprises: using a synthetic substrate bringing either the reaction product formed by enzyme reaction or the unreacted synthetic substrate into contact with silver halide, developing the same, and measuring optical density of the formed silver image and/or colored dye, the synthetic substrate comprises at least one structure (A) which is specifically contacted with an enzyme to be measured and at least one photographically fogging agent structure (B) in the molecule thereof.
摘要翻译：在微量酶的光化学测定方法中，包括：使用将酶反应形成的反应产物或未反应的合成基质与卤化银接触的合成底物，显影，并测量形成的银影像的光密度和/ 或着色染料，合成底物包含至少一种与待测酶特异性接触的结构（A）和其分子中的至少一种照相起雾剂结构（B）。

28. 发明授权

US4331444A Competitive immunoassay using silver halide fogging agent 失效
标题翻译：使用卤化银雾化剂进行竞争性免疫测定
公开(公告)号：US4331444A
公开(公告)日：1982-05-25
申请号：US126919
申请日：1980-03-03
申请人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi Miyazako , Shigeo Hirano
发明人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi Miyazako , Shigeo Hirano
IPC分类号： G01N33/536 , G01N33/537 , G01N33/54 , G01N33/58
CPC分类号： G01N33/537 , Y10S436/805
摘要： In a method for the immunological analysis of trace components by marking or labelling an antigen or antibody with a marker, an immune reaction is caused using an antigen or antibody marked with a fogging agent for silver halide, the labelled antigen or antibody is separated from the labelled antigen-antibody reaction product, the silver halide is developed in the presence of either one of the labelled antigen or antibody and the labelled antigen-antibody reaction product, and the density obtained is measured.The method is comparable to radioimmunoassay in having high reproductibility and sufficient sensitivity but does not involve any risk due to radiation.
摘要翻译：在通过用标记物标记或标记抗原或抗体来对痕量组分的免疫分析的方法中，使用用卤化银的起雾剂标记的抗原或抗体引起免疫反应，将标记的抗原或抗体从标记的抗原 - 抗体反应产物，在标记的抗原或抗体之一和标记的抗原 - 抗体反应产物的存在下显影卤化银，并测量获得的密度。该方法与放射免疫测定相当，具有高的可再生性和足够的灵敏度，但不涉及由于辐射引起的任何风险。

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