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1. 发明申请

US20070275429A1 Method for measuring high density lipoprotein cholesterol 有权
标题翻译：高密度脂蛋白胆固醇测定方法
公开(公告)号：US20070275429A1
公开(公告)日：2007-11-29
申请号：US11798141
申请日：2007-05-10
申请人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
发明人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , G01N33/92
摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
摘要翻译：本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件用于HDL-C检测。本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。和来源于假单胞菌属的胆固醇氧化酶。用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。

2. 发明授权

US08859221B2 Method for measuring high density lipoprotein cholesterol 有权
标题翻译：高密度脂蛋白胆固醇测定方法
公开(公告)号：US08859221B2
公开(公告)日：2014-10-14
申请号：US11798141
申请日：2007-05-10
申请人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
发明人： Yoshihiko Abe , Nobuhito Masuda , Hiroko Inomata
IPC分类号： C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92
摘要： An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
摘要翻译：本发明要达到的目的是提供一种通过使用便宜的材料方便，快速，具体地测量样品中的HDL-C的方法，并提供用于HDL-C检测的试剂盒和干燥分析元件用于HDL-C检测。本发明提供了一种测定体液测试样品中的高密度脂蛋白胆固醇（HDL-C）的方法，其中来源于裂殖酵母（Schizophyllum commune）或假单胞菌属（Pseudomonas sp。）的胆固醇酯酶。和来源于假单胞菌属的胆固醇氧化酶。用于从HDL-C产生过氧化氢，从而选择性地测量HDL-C。

3. 发明申请

US20090246811A1 DRY ANALYTICAL ELEMENT FOR LIPASE MEASUREMENT 有权
标题翻译：用于脂肪测量的干燥分析元素
公开(公告)号：US20090246811A1
公开(公告)日：2009-10-01
申请号：US12410035
申请日：2009-03-24
申请人： Jun Arakawa , Nobuhito Masuda , Kentaro Nakamura , Shigeki Kageyama
发明人： Jun Arakawa , Nobuhito Masuda , Kentaro Nakamura , Shigeki Kageyama
IPC分类号： C12Q1/44
CPC分类号： C12Q1/44 , G01N2333/92
摘要： The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 μm or less.
摘要翻译：本发明提供了含有含有碳原子数12〜22的长链烷基脂肪酸甘油三酯，单甘油酯脂肪酶，甘油测定试剂的体液中所含的胰脂肪酶的测定用干燥分析元件的制造方法，水不渗透性载体和至少一种扩散剂或试剂层，所述方法包括涂覆平均粒度为1μm以下的甘油三酯的乳液/分散液的步骤。

4. 发明授权

US5266460A Method of preparing immunological analytical element 失效
标题翻译：免疫学分析元素的制备方法
公开(公告)号：US5266460A
公开(公告)日：1993-11-30
申请号：US887425
申请日：1992-05-20
申请人： Yukio Sudo , Nobuhito Masuda
发明人： Yukio Sudo , Nobuhito Masuda
IPC分类号： G01N33/543 , C12Q1/25 , G01N33/535
CPC分类号： G01N33/54386 , Y10S435/969 , Y10S435/97
摘要： A method of preparing a dry-type analytical element for immunoassay having at least one water-permeable porous layer and containing a labeled antigen and an antibody to said labeled antigen and to an unlabeled antigen as the components participating in antigen-antibody reaction in said porous layer, which comprises applying or impregnating a first composition containing one of said labeled antigen and said antibody and thereafter applying or impregnating a second composition containing the other of said labeled antigen and said antibody dissolved or suspended in a solvent not dissolving substantially said labeled antigen or said antibody in the first composition onto said porous layer.
摘要翻译：一种制备用于免疫测定的干式分析元件的方法，所述干式分析元件具有至少一个透水性多孔层，并且含有标记的抗原和对所述标记的抗原的抗体以及未标记的抗原作为参与所述多孔的抗原 - 抗体反应的成分其包括施用或浸渍含有所述标记的抗原和所述抗体之一的第一组合物，然后施用或浸渍含有所述标记的抗原和所述抗体中的另一种的第二组合物，所述第二组合物溶解或悬浮在基本上不溶解所述标记抗原的溶剂中，或者所述第一组合物中的抗体在所述多孔层上。

5. 发明授权

US4855108A Member of analytical element for the analysis of liquid sample containing solid 失效
标题翻译：用于分析含有固体的液体样品的分析元素成员
公开(公告)号：US4855108A
公开(公告)日：1989-08-08
申请号：US98735
申请日：1987-09-16
申请人： Nobuhito Masuda , Takeshi Igarashi
发明人： Nobuhito Masuda , Takeshi Igarashi
IPC分类号： G01N33/52
CPC分类号： G01N33/525 , Y10S435/805 , Y10T436/25375
摘要： A member of an analytical element for the analysis of a liquid sample containing solid material, comprising a volume filtration layer composed of a fibrous material and a spreading layer having a liquid-retaining capacity larger than that of the volume filtration layer, in which the volume filtration layer and the spreading layer are united to give an integrated structure. The spreading layer may be composed of a fibrous material, a woven cloth, a knitted cloth or a non-fibrous porous medium.
摘要翻译：用于分析含有固体材料的液体样品的分析元件的成员，其包括由纤维材料构成的体积过滤层和具有比体积过滤层的液体保持能力大的液体保持能力的扩散层，其中体积过滤层和扩散层结合起来形成一体的结构。铺展层可以由纤维材料，织布，编织布或非纤维多孔介质组成。

6. 发明申请

US20120034706A1 METHOD FOR ASSESSING INFLAMMATORY CONDITION 审中-公开
标题翻译：评估炎症条件的方法
公开(公告)号：US20120034706A1
公开(公告)日：2012-02-09
申请号：US12964077
申请日：2010-12-09
申请人： Yukitaka Ueki , Nobuhito Masuda , Kyoko Miyamoto , Chihiro Miyazawa , Yukio Sudo
发明人： Yukitaka Ueki , Nobuhito Masuda , Kyoko Miyamoto , Chihiro Miyazawa , Yukio Sudo
IPC分类号： G01N33/53
CPC分类号： G01N33/6893 , G01N2333/4737 , G01N2800/102
摘要： Provided a method for correctly assessing an inflammatory condition of a patient who is receiving a therapy with an IL-6 inhibitor. The method for assessing an inflammatory condition of a patient who is receiving an IL-6 inhibitor, including determining a PTX3 level of a sample derived from a patient who is receiving an IL-6 inhibitor.
摘要翻译：提供了正确评估接受IL-6抑制剂治疗的患者的炎症状态的方法。用于评估正在接受IL-6抑制剂的患者的炎症状态的方法，包括确定来自接受IL-6抑制剂的患者的样品的PTX3水平。

7. 发明授权

US4473652A Method and immunochemical measurement 失效
标题翻译：方法和免疫化学测量
公开(公告)号：US4473652A
公开(公告)日：1984-09-25
申请号：US506225
申请日：1983-06-22
申请人： Masaki Okazaki , Nobuhito Masuda , Yoshiro Kumano
发明人： Masaki Okazaki , Nobuhito Masuda , Yoshiro Kumano
IPC分类号： G01N33/58 , G01N33/54
CPC分类号： G01N33/583
摘要： A method for immunochemical assay of an antigen or antibody by labelling the antigen or antibody with a specific cyanine or merocyanine dye containing a carboxy group followed by effecting an immune reaction and photochemical processing thereof is provided. The amount of the antigen or antibody is measured in term of optical density of developed silver halide which is brought into contact with either the antigen-antibody reaction product or the unreacted material.This immunochemical assay method gives high detection sensitivity in a simple operation manner.
摘要翻译：提供了通过用含有羧基的特定花青或部花青染料标记抗原或抗体，然后进行免疫反应和其光化学处理来对抗原或抗体进行免疫化学测定的方法。根据与抗原抗体反应产物或未反应物质接触的显影卤化银的光密度来测量抗原或抗体的量。该免疫化学测定方法以简单的操作方式提供高检测灵敏度。

8. 发明授权

US08906641B2 Method for measuring animal α-amylase 有权
标题翻译：测定动物α-淀粉酶的方法
公开(公告)号：US08906641B2
公开(公告)日：2014-12-09
申请号：US12015151
申请日：2008-01-16
申请人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
发明人： Shigeki Kageyama , Nobuhito Masuda , Kazuya Kawasaki , Satoru Toda
IPC分类号： C12Q1/40 , G01N33/52
CPC分类号： C12Q1/40 , G01N33/523 , G01N33/526
摘要： It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring α-amylase even in a sample comprising animal α-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.
摘要翻译：本发明的目的是提供一种能够测量高浓度（高达2500U / L）的动物样品而不稀释的方法，即使在包含动物α-淀粉样品的样品中也能够特异性地测量α-淀粉酶，淀粉酶（EC 3.2.1.1）和葡糖淀粉酶（EC 3.2.1.3）。本发明提供一种用于特异性测量包含α-淀粉酶和葡糖淀粉酶的非人动物样品中α-淀粉酶的方法，而不稀释所述样品，其中通过使用具有受保护的非还原末端的寡糖和对硝基苯在其还原末端，反应的pH为6以上且小于7。

9. 依法登记的发明

USH1664H Analytical element for immunoassay and method for its preparation 失效
标题翻译：免疫测定的分析元素及其制备方法
公开(公告)号：USH1664H
公开(公告)日：1997-07-01
申请号：US320771
申请日：1989-03-08
申请人： Yukio Sudo , Nobuhito Masuda
发明人： Yukio Sudo , Nobuhito Masuda
IPC分类号： G01N33/543
CPC分类号： G01N33/54386
摘要： A dry-type analytical element for immunoassay utilizing antigen-antibody reaction in which the antigenic component is isolated from the antibody component by incorporating both components in separate layers or separately in a layer using different solvents. The analytical element is excellent in sensitivity and accuracy, and the analytical operation is simple.
摘要翻译：用于使用抗原 - 抗体反应进行免疫测定的干式分析元件，其中通过将两种组分并入分开的层中，或者使用不同溶剂在一层中分别地将抗原成分从抗体成分中分离出来。分析元件的灵敏度和精度都很好，分析操作简单。

10. 发明授权

US4861552A Biological reaction layer and its preparation 失效
标题翻译：生物反应层及其制备
公开(公告)号：US4861552A
公开(公告)日：1989-08-29
申请号：US851878
申请日：1986-04-11
申请人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
发明人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
IPC分类号： G01N33/52 , C12M1/00 , G01N33/543 , G01N33/78 , C12Q1/00 , G01N33/548
CPC分类号： G01N33/78 , G01N33/54386 , Y10S435/805 , Y10S436/81
摘要： A biological reaction layer of a dry analytical element in the form of a composite porous layer in which a particulate material carrying a biologically active material fixed thereon is dispersed in a fibrous porous matrix, characterized in that the dry weight ratio of said particulate material to said fibrous material contained in said composite porous layer ranges from 1:20 to 1:0.3 and said particulate material is contained therein in the range of 1 to 60 g/m.sup.2.
摘要翻译：复合多孔层形式的干燥分析元件的生物反应层，其中载有固定在其上的生物活性材料的颗粒材料分散在纤维多孔基质中，其特征在于，所述颗粒材料与所述所述复合多孔层中含有的纤维状物质的含量为1:20〜1:0.3，所述粒状物质的含量为1〜60g / m 2。

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