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    • 23. 发明申请
    • Thermostable polymerases having altered fidelity and methods of identifying and using same
    • 具有改变的保真度的热稳定性聚合酶以及鉴定和使用它们的方法
    • US20050196800A1
    • 2005-09-08
    • US11096645
    • 2005-03-31
    • Lawrence LoebLeroy HoodMotoshi Suzuki
    • Lawrence LoebLeroy HoodMotoshi Suzuki
    • C12N9/10C12N9/12C12N9/22C12P19/34C12Q1/34C12Q1/68
    • C12N9/1252C12Q1/6858C12Q1/6883
    • The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.
    • 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如,本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸,例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。
    • 24. 发明授权
    • Resource management system and digital video reproducing/recording apparatus
    • 资源管理系统和数字视频再现/记录装置
    • US06704489B1
    • 2004-03-09
    • US09558119
    • 2000-04-25
    • Nobukazu KurauchiNarutoshi AgeishiMotoshi SuzukiKazuo Kajimoto
    • Nobukazu KurauchiNarutoshi AgeishiMotoshi SuzukiKazuo Kajimoto
    • H04N593
    • H04N5/765H04N5/775H04N5/781H04N5/9261
    • A resource management system is composed of a plurality of application programs, a resource management information database, and a resource manager. Each application program executes data transfer using resources. The resource management database stores resource management information and key information for each resource. Upon receipt of a resource use request from an application program, the resource manager judges whether the requested resource can be allocated to the application program by referring to the resource capacity and the currently-allocated volume of the resource that are included in the information database. If judging that the requested resource can be allocated, the resource manager gives authorization to the application program to use the resource. The resource manager also controls resource allocation by restricting a data transfer bandwidth used by an application program and requesting a resource release on expiration of the valid time period.
    • 资源管理系统由多个应用程序,资源管理信息数据库和资源管理器构成。 每个应用程序使用资源执行数据传输。 资源管理数据库存储每个资源的资源管理信息和密钥信息。 在从应用程序接收到资源使用请求时,资源管理器通过参考信息数据库中包括的资源的资源容量和当前分配的资源来判断所请求资源是否可以分配给应用程序。 如果判断可以分配所请求的资源,则资源管理器授予应用程序来使用该资源。 资源管理器还通过限制应用程序使用的数据传输带宽并在有效时间段到期时请求资源释放来控制资源分配。