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21. 发明申请

US20110160077A1 SEQUENCING METHODS USING ENZYME CONFORMATION 有权
标题翻译：使用酶合成的序列方法
公开(公告)号：US20110160077A1
公开(公告)日：2011-06-30
申请号：US12967507
申请日：2010-12-14
申请人： Mark Chaisson , Sonya Clark , Christopher Silk , John Lyle , Lei Jia , Keith Bjornson
发明人： Mark Chaisson , Sonya Clark , Christopher Silk , John Lyle , Lei Jia , Keith Bjornson
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： C12Q1/6869 , C12Q2521/101 , C12Q2561/113 , C12Q2563/107
摘要： Systems and methods are provided for single-molecule sequencing of template nucleic acids in which the signal from a label attached to a polymerase enzyme is used to monitor conformational changes in the polymerase which occur while labeled nucleotides or nucleotide analogs are added to a growing nucleic acid chain which is complementary to the template nucleic acid. The signal indicative of the conformational state of the enzyme is used to determine with higher confidence when true nucleotide or nucleotide analog incorporation events occur, allowing for the improved quality of base calls and sequence determination.
摘要翻译：提供了用于模板核酸的单分子测序的系统和方法，其中来自连接到聚合酶的标记的信号用于监测聚合酶中的构象变化，同时将标记的核苷酸或核苷酸类似物加入生长的核酸与模板核酸互补的链。当发生真核苷酸或核苷酸类似物并入事件时，使用指示酶构象状态的信号来确定更高的置信度，从而提高碱基调用质量和序列测定。

22. 发明申请

US20090181396A1 METHODS AND SYSTEMS FOR ANALYSIS OF FLUORESCENT REACTIONS WITH MODULATED EXCITATION 有权
标题翻译：用调制激发荧光反应分析的方法与系统
公开(公告)号：US20090181396A1
公开(公告)日：2009-07-16
申请号：US12351173
申请日：2009-01-09
申请人： Khai Luong , Paul Lundquist , Ravi Dalal , John Lyle , Stephen Turner
发明人： Khai Luong , Paul Lundquist , Ravi Dalal , John Lyle , Stephen Turner
IPC分类号： C12Q1/68 , G01N21/76 , C12M1/34
CPC分类号： C12M1/34 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/7786 , Y10T436/2525
摘要： Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.
摘要翻译：用于监测来自感兴趣的反应混合物的荧光信号和特别是瞬时荧光信号的方法，系统及其组件，该方法和系统采用调制的激发光源以减少过度照明对反应组分的影响或由其获得的数据。

23. 发明授权

US08802424B2 Methods and systems for analysis of fluorescent reactions with modulated excitation 有权
标题翻译：用调制激发分析荧光反应的方法和系统
公开(公告)号：US08802424B2
公开(公告)日：2014-08-12
申请号：US12351173
申请日：2009-01-09
申请人： Khai Luong , Paul Lundquist , Ravi Dalal , John Lyle , Stephen Turner
发明人： Khai Luong , Paul Lundquist , Ravi Dalal , John Lyle , Stephen Turner
IPC分类号： C12M1/00 , C12Q1/68 , C12M1/34 , G01N21/64 , G01N1/00
CPC分类号： C12M1/34 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2021/7786 , Y10T436/2525
摘要： Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.
摘要翻译：用于监测来自感兴趣的反应混合物的荧光信号和特别是瞬时荧光信号的方法，系统及其组件，该方法和系统采用调制的激发光源以减少过度照明对反应组分的影响或从其获得的数据。

24. 发明授权

US08551704B2 Controllable strand scission of mini circle DNA 有权
标题翻译：迷你环DNA的可控链断裂
公开(公告)号：US08551704B2
公开(公告)日：2013-10-08
申请号：US12029273
申请日：2008-02-11
申请人： Geoffrey Otto , John Lyle
发明人： Geoffrey Otto , John Lyle
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q2525/307 , C12Q2525/186
摘要： The invention provides methods and compositions for the controlled termination of polymerase mediated primer extension reactions. The methods and compositions of the invention are broadly useful, and in a preferred aspect can be used in identifying sequence elements of template nucleic acids. Control of termination not only provides temporal control over termination, but, when used in conjunction with optically confined reaction regions, also spatially controls such termination.
摘要翻译：本发明提供了用于受控终止聚合酶介导的引物延伸反应的方法和组合物。本发明的方法和组合物是广泛有用的，并且在优选的方面可用于鉴定模板核酸的序列元件。终止控制不仅提供对终止的时间控制，而且当与光学限制反应区一起使用时，也在空间上控制这种终止。

25. 发明申请

US20070167204A1 Character for computer game and method 有权
标题翻译：电脑游戏和方法的角色
公开(公告)号：US20070167204A1
公开(公告)日：2007-07-19
申请号：US11329578
申请日：2006-01-11
申请人： John Lyle , Brenton Lyle , Robert Ellsworth
发明人： John Lyle , Brenton Lyle , Robert Ellsworth
IPC分类号： A63F9/24
CPC分类号： A63F13/79 , A63F13/12 , A63F13/65 , A63F13/822 , A63F2300/5506 , A63F2300/5546 , A63F2300/5553 , A63F2300/695
摘要： A video game character or avatar is generated using images of the player so that the on-screen avatar being controlled by the player appears like the player. Answers to a questionnaire or a psychological profile are provided by the player to determine characteristics that the player views as desirable, which are used to generate a final version of the avatar. Iterations of the avatar appearance are generated from the initial appearance to the final, more desirable appearance. Using the avatar, as play of the game proceeds the player's avatar begins as a character appearing like themselves and gradually becomes a character that is more as they would like to appear. The avatar increases the emotional impact of games by providing strong visual and psychological connections with the player. The avatar may instead begin from an initial character that does not appear similar to the player. The iterations may make the avatar gradually less appealing or with other changes in appearance, in some embodiments.
摘要翻译：使用播放器的图像生成视频游戏角色或头像，使得由玩家控制的屏幕上的头像看起来像播放器。玩家提供问卷或心理资料的答案，以确定玩家视为期望的特征，用于生成化身的最终版本。化身外观的迭代从最初的外观生成到最终的，更理想的外观。使用头像，随着游戏的进行，玩家的头像开始为像自己一样的角色，并逐渐变成更像他们想要出现的角色。头像通过与玩家提供强烈的视觉和心理联系来增加游戏的情感影响。头像可以改为从与玩家不相似的初始角色开始。在一些实施例中，迭代可以使化身逐渐变得不吸引人或外观上的其他改变。

26. 发明授权

US08921086B2 Polymerases for nucleotide analogue incorporation 有权
标题翻译：用于核苷酸类似物掺入的聚合酶
公开(公告)号：US08921086B2
公开(公告)日：2014-12-30
申请号：US11645223
申请日：2006-12-21
申请人： David K. Hanzel , Geoff Otto , Paul Peluso , Thang Pham , David R. Rank , Paul Mitsis , Fred Christians , Arek Bibillo , Insil Park , Sonya Clark , John Lyle
发明人： David K. Hanzel , Geoff Otto , Paul Peluso , Thang Pham , David R. Rank , Paul Mitsis , Fred Christians , Arek Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
摘要翻译：提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。

27. 发明申请

US20100136592A1 Photo-Induced Damage Mitigating Agents and Preparation and Methods of Use Thereof 有权
标题翻译：光诱导减伤剂及其制备及使用方法
公开(公告)号：US20100136592A1
公开(公告)日：2010-06-03
申请号：US12622375
申请日：2009-11-19
申请人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
发明人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
IPC分类号： C12Q1/48 , C12N9/00 , C12N11/00 , C12N9/12 , C12Q1/00 , C07H19/04 , C12N9/96 , C12M1/34 , C09K15/08
CPC分类号： C07C49/255 , G01N21/6428 , G01N33/5306 , G01N2021/6432
摘要： Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
摘要翻译：用于通过向反应混合物中加入一种或多种光诱导的损伤缓解剂来减少和/或防止一种或多种反应物在照明分析反应中的光诱导损伤的组合物，装置，系统和方法，并允许反应进行这个时间小于光致伤害阈值期。

28. 发明申请

US20080009007A1 CONTROLLED INITIATION OF PRIMER EXTENSION 审中-公开
标题翻译：控制引擎扩展
公开(公告)号：US20080009007A1
公开(公告)日：2008-01-10
申请号：US11763746
申请日：2007-06-15
申请人： John Lyle , Paul Peluso , Gene Shen
发明人： John Lyle , Paul Peluso , Gene Shen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2561/113 , C12Q2525/186 , C12Q2523/313
摘要： Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking, groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.
摘要翻译：通过掺入核苷酸或核苷酸类似物来测定核酸序列信息中引物延伸的控制引发。优选的方面包括通过使用光可切割阻断的光引发的延伸，引物序列的末端的基团，随后使用不是延伸终止子的核苷酸或核苷酸类似物的非终止引物延伸。

29. 外观设计

USD274669S Undergarment 失效
公开(公告)号：USD274669S
公开(公告)日：1984-07-17
申请号：US400444
申请日：1982-07-21
申请人： John Lyle
设计人： John Lyle

30. 发明授权

US08388982B2 Photo-induced damage mitigating agents and preparation and methods of use thereof 有权
标题翻译：光诱导的损伤缓解剂及其制备方法及其使用方法
公开(公告)号：US08388982B2
公开(公告)日：2013-03-05
申请号：US12622375
申请日：2009-11-19
申请人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
发明人： Xiangxu Kong , Gene Shen , Andrei Fedorov , John Lyle , Grace Lee , Lubomir Sebo , Duc Do , Robert Weber , Stephen Dudek
IPC分类号： A61K9/00 , A61K8/00 , A61F13/00 , C12Q1/68 , C12Q1/48 , C12Q1/02
CPC分类号： C07C49/255 , G01N21/6428 , G01N33/5306 , G01N2021/6432
摘要： Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photo-induced damage mitigating agents to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
摘要翻译：用于通过向反应混合物中加入一种或多种光诱导的损伤缓解剂来减少和/或防止一种或多种反应物在照明分析反应中的光诱导损伤的组合物，装置，系统和方法，并允许反应进行这个时间小于光致伤害阈值周期。

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