专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明授权

US07297499B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07297499B2
公开(公告)日：2007-11-20
申请号：US11489582
申请日：2006-07-20
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2535/125 , C12Q2525/185
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T; the third base from the 3′ end to be any one of T, G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T; 3'末端的第3个碱基为T，G或C任一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

22. 发明申请

US20070082344A1 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
公开(公告)号：US20070082344A1
公开(公告)日：2007-04-12
申请号：US11363953
申请日：2006-03-01
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2535/125 , C12Q2525/185
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is A. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

23. 发明授权

US07055540B2 Method of moving fluid in capillary chip 有权
标题翻译：在毛细管芯片中移动流体的方法
公开(公告)号：US07055540B2
公开(公告)日：2006-06-06
申请号：US11195649
申请日：2005-08-03
申请人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
发明人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
IPC分类号： E03B1/00 , F15C1/00
CPC分类号： G01N27/44791 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0816 , B01L2300/0864 , B01L2400/0661 , F16K99/0001 , F16K99/0017 , F16K99/0036 , F16K2099/0074 , F16K2099/0084 , Y10T137/0318 , Y10T137/206 , Y10T137/2196 , Y10T137/2224
摘要： The present invention is a method of moving a fluid in a capillary using a capillary chip having a layer including a polymer composition and a capillary formed on the surface or inside of the aforementioned layer including a polymer composition, wherein the aforementioned capillary has a movement control part, and the aforementioned movement control part includes multiple and sequential opening/closing parts; the aforementioned opening/closing part blocks movement of the fluid that flows in the aforementioned capillary by increase in the volume of the aforementioned polymer composition to result in the closed state, while it permits movement of the fluid that flows in the aforementioned capillary by decrease in the volume of the aforementioned polymer composition to result in the open state; and the aforementioned method of moving the fluid includes a step (a) of switching the aforementioned multiple opening/closing parts from the open state to the closed state sequentially in a movement direction by changing the temperature of the aforementioned polymer composition in the aforementioned movement control part, thereby moving the fluid in the aforementioned capillary.
摘要翻译：本发明是一种使用毛细管芯片的毛细管芯片移动流体的方法，所述毛细管芯片具有包含聚合物组合物和形成在包括聚合物组合物的上述层的表面或内部的毛细管的毛细管，其中上述毛细管具有移动控制上述移动控制部分包括多个和顺序的打开/关闭部分; 上述打开/关闭部分通过增加上述聚合物组合物的体积来阻止在上述毛细管中流动的流体的运动，导致关闭状态，同时允许通过减少在上述毛细管中流动的流体的运动上述聚合物组合物的体积导致打开状态; 并且上述移动流体的方法包括以下步骤：（a）通过在上述移动控制中改变上述聚合物组合物的温度，将上述多个打开/关闭部分从打开状态顺序地切换到关闭状态从而使上述毛细管中的流体移动。

24. 发明授权

US06890762B1 Method for measuring physiocochemical properties of tissues of cells, method for examining chemicals, and apparatus therefor 失效
标题翻译：用于测量细胞组织的生理化学性质的方法，用于检查化学品的方法及其装置
公开(公告)号：US06890762B1
公开(公告)日：2005-05-10
申请号：US08913811
申请日：1997-01-24
申请人： Hirokazu Sugihara , Yasushi Kobayashi , Hiroaki Oka , Ryuta Ogawa , Makoto Taketani
发明人： Hirokazu Sugihara , Yasushi Kobayashi , Hiroaki Oka , Ryuta Ogawa , Makoto Taketani
IPC分类号： C12Q1/02 , G01N33/50 , G01N33/567 , C12Q1/04 , G01N33/48 , G01N33/53 , C12N27/00
CPC分类号： G01N33/5005 , C12Q1/02 , Y10S436/806 , Y10S436/807
摘要： A method of measuring the physical and chemical properties of tissue or cells and a device for the same is provided, with which the physical and chemical environment of the tissue or cells can be changed arbitrarily corresponding to experimental necessities. The device comprises a system 40 for keeping the physical and chemical environment surrounding the biological tissue or cells constant, a system 50 for arbitrarily changing the physical and chemical environment, observation systems 10 and 20 for observing the physical and chemical properties of the tissue or cells, and a system 30 for comparing the change of the physical and chemical properties of the tissue or cells before and after changing the physical and chemical environment. The observation system 10 is a potential measurement device for measuring the electrophysiological properties of the tissue or cells. This device comprises an integrated cell placement device 1 provided with a plurality of microelectrodes 11 on a substrate, a cell placement portion 6 for placing the tissue or cells on the microelectrodes 11, and a wiring pattern 12 for applying an electrical signal to the microelectrodes 11 and extracting an electrical signal from the microelectrodes 11.
摘要翻译：提供了一种测量组织或细胞的物理和化学性质的方法及其装置，通过该方法可以根据实验需要任意改变组织或细胞的物理和化学环境。该装置包括用于保持生物组织或细胞周围的物理和化学环境恒定的系统40，用于任意改变物理和化学环境的系统50，用于观察组织或细胞的物理化学性质的观察系统10和20 以及用于比较在改变物理和化学环境之前和之后组织或细胞的物理和化学性质的变化的系统30。观察系统10是用于测量组织或细胞的电生理特性的电位测量装置。该装置包括在基板上设置有多个微电极11的集成电池放置装置1，用于将组织或电池放置在微电极11上的电池放置部分6以及用于向微电极11施加电信号的布线图案12 以及从微电极11提取电信号。

25. 发明授权

US6132683A Cell potential measuring electrode and measuring apparatus using the same 有权
公开(公告)号：US6132683A
公开(公告)日：2000-10-17
申请号：US220981
申请日：1998-12-23
申请人： Hirokazu Sugihara , Hiroaki Oka , Ken Shimono , Ryuta Ogawa , Makoto Taketani
发明人： Hirokazu Sugihara , Hiroaki Oka , Ken Shimono , Ryuta Ogawa , Makoto Taketani
IPC分类号： C12M1/34 , G01N33/487 , G01N27/26
CPC分类号： C12M41/46 , G01N33/4836
摘要： This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.

26. 发明授权

US5645846A Method of disinfecting an object and antiviral disinfection liquid therefor 失效
标题翻译：消毒物品及其抗病毒消毒液的方法
公开(公告)号：US5645846A
公开(公告)日：1997-07-08
申请号：US582786
申请日：1996-01-04
申请人： Hiroaki Oka , Toshikazu Tomioka , Katsumi Tomita , Kenji Hoshino , Atsushi Nishino , Shigeharu Ueda
发明人： Hiroaki Oka , Toshikazu Tomioka , Katsumi Tomita , Kenji Hoshino , Atsushi Nishino , Shigeharu Ueda
IPC分类号： A01N59/16 , A01N59/20 , A61K9/16
CPC分类号： A01N59/16 , A01N59/20
摘要： An antiviral composition comprising: a thiosulfate salt and at least one of thiosulfate complex salt of a metal and a porous particulate carrier; said metal being at least one selected from the group consisting of silver, copper and zinc: said salts being carried on said porous particulate carrier. It is applicable to a wide range of living environment such as medical instruments and appliances, hygienic implements as well as kitchen utensils, and also demonstrates an antibacterial activity in addition to the antiviral activity. The composition releases its salts slowly to the environment and the antiviral and antibacterial activities of said composition lasts for a long period of time. A method for preparing said composition as well as an antiviral disinfection method and a virucidal disinfection fluid using said composition are also disclosed.
摘要翻译：一种抗病毒组合物，其包含：硫代硫酸盐和金属的硫代硫酸盐配合盐和多孔颗粒载体中的至少一种; 所述金属为选自银，铜和锌中的至少一种金属：所述盐载于所述多孔颗粒载体上。它适用于各种生活环境，如医疗仪器和电器，卫生用具以及厨房用具，除抗病毒活性外，还具有抗菌活性。该组合物缓慢地将其盐释放到环境中，并且所述组合物的抗病毒和抗菌活性持续很长时间。还公开了使用所述组合物制备所述组合物的方法以及抗病毒消毒方法和杀病毒消毒液。

27. 发明授权

US08822165B2 Method for producing cAMP using chimeric olfactory receptor 有权
标题翻译：使用嵌合嗅觉受体制备cAMP的方法
公开(公告)号：US08822165B2
公开(公告)日：2014-09-02
申请号：US13598294
申请日：2012-08-29
申请人： Masato Suzuki , Hiroaki Oka , Shigeki Kiyonaka , Yasuo Mori
发明人： Masato Suzuki , Hiroaki Oka , Shigeki Kiyonaka , Yasuo Mori
IPC分类号： C07K14/705 , C07K19/00 , G01N33/566 , C12P19/32 , C07K14/47 , C07K14/72 , G01N33/68
CPC分类号： C07K14/705 , C07K14/47 , C07K14/70571 , C07K14/723 , C07K2319/00 , C07K2319/41 , C12P19/32 , G01N33/6872 , G01N2333/726 , G01N2500/04
摘要： A method for producing cAMP using a chimeric olfactory receptor. The method includes a step of preparing a reaction system comprising a first layer, a lipid bilayer membrane, and a second layer, and a step of supplying a chemical substance which stimulates the chimeric olfactory receptor to the first layer so as to produce the cAMP from ATP. The lipid bilayer membrane includes the chimeric olfactory receptor and adenylate cyclase. The chimeric olfactory receptor penetrates the lipid bilayer membrane. The second layer contains ATP and a G protein. The G protein is placed in the vicinity of one end of the chimeric olfactory receptor. The chimeric olfactory receptor is derived from a mouse olfactory receptor and the N-terminal of the chimeric olfactory receptor is modified with an amino acid sequence.
摘要翻译：使用嵌合嗅觉受体制备cAMP的方法。该方法包括制备包括第一层，脂质双层膜和第二层的反应体系的步骤，以及将刺激嵌合嗅觉受体的化学物质提供给第一层以产生cAMP的步骤 ATP。脂质双层膜包括嵌合嗅觉受体和腺苷酸环化酶。嵌合嗅觉受体渗透脂质双层膜。第二层含有ATP和G蛋白。将G蛋白置于嵌合嗅觉受体的一端附近。嵌合嗅觉受体来自小鼠嗅觉受体，嵌合嗅觉受体的N-末端用氨基酸序列修饰。

28. 发明授权

US08435393B2 Instrument and system for pharmacologic measurement and well vessel used therein 有权
标题翻译：用于药物测量的仪器和系统及其中使用的井筒
公开(公告)号：US08435393B2
公开(公告)日：2013-05-07
申请号：US12692104
申请日：2010-01-22
申请人： Nobuhiko Ozaki , Hiroaki Oka
发明人： Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： G01N33/487
CPC分类号： G01N33/48728
摘要： An instrument is provided for pharmacologic measurement capable of detecting a very small and short time change in electric signal caused by a pharmacologic action of a biologic specimen with quickness and a high sensitivity by steeply decreasing an external disturbance component mixed into the system while dropping/exchange of medicinal solutions is conducted in a batch mode. The pharmacologic measurement instrument detects a change in electric signal caused by a pharmacologic action or electrophysiologic action of a biologic specimen, and includes an electrically conductive box having an opening section at the top surface thereof and a well vessel disposed in the opening section thereof, wherein the well vessel includes a base having recesses in which the biologic specimens are put; measurement electrodes formed on the bottom surfaces of the respective recesses; and reference electrodes electrically insulated from the respective measurement electrodes, and the reference electrodes together with the electrically conductive box electrostatically shields the well vessel.
摘要翻译：提供了用于药物测量的仪器，其能够通过快速和高灵敏度检测由生物样品的药理作用引起的电信号的非常小的和短的时间变化，通过急剧降低混合到系统中的外部干扰成分，同时进行下降/交换药物溶液以批式进行。药理测量仪器检测由生物样本的药理作用或电生理作用引起的电信号变化，并且包括其顶表面具有开口部分的导电盒和设置在其开口部分中的井管，其中井筒包括具有其中放置生物样本的凹部的基部; 形成在各个凹部的底面上的测量电极; 以及与各测量电极电绝缘的参考电极，并且参考电极与导电盒一起静电屏蔽阱容器。

29. 发明申请

US20130045559A1 METHOD FOR MANUFACTURING SOLAR CELL USING SILICON POWDER 失效
标题翻译：使用硅粉制造太阳能电池的方法
公开(公告)号：US20130045559A1
公开(公告)日：2013-02-21
申请号：US13591628
申请日：2012-08-22
申请人： Hiroaki Oka , Nariaki Oka
发明人： Hiroaki Oka , Nariaki Oka
IPC分类号： H01L31/18
CPC分类号： H01L31/1804 , C23C24/10 , H01L31/068 , Y02E10/547 , Y02P70/521
摘要： To provide a method for manufacturing a solar cell, whereby solar cells can be mass-produced by a simple process at low cost. A first conductivity-type silicon powder (11) is prepared, a silicon powder layer (11a) is formed by disposing the powder in the form of a layer, the powder layer is melted by heating the powder layer to the melting point of silicon or higher, and a first conductivity-type silicon layer (11b) is formed by cooling the melted layer. A second conductivity-type silicon powder (12) is prepared, a second conductivity-type silicon powder layer (12a) is formed by disposing the powder in the form of a layer on the first conductivity-type silicon layer (11b), the powder layer is melted by heating the powder layer to the melting point of silicon or higher, and a second conductivity-type silicon layer (12b) is formed by cooling the melted layer.
摘要翻译：为了提供太阳能电池的制造方法，能够以简单的方法以低成本大量生产太阳能电池。制备第一导电型硅粉末（11），通过将粉末以层的形式设置形成硅粉末层（11a），通过将粉末层加热至硅的熔点或熔融温度并且通过冷却熔融层形成第一导电型硅层（11b）。制备第二导电型硅粉末（12），通过在第一导电型硅层（11b）上设置层状的粉末形成第二导电型硅粉末层（12a），粉末通过将粉末层加热到硅的熔点以上来熔融层，并且通过冷却熔融层形成第二导电型硅层（12b）。

30. 发明申请

US20120083001A1 METHOD FOR TRANSPORTING POTASSIUM IONS FROM FRONT SIDE TO BACK SIDE OF LIPID BILAYER MEMBRANE 有权
标题翻译：从前端向脂质双层膜的背面输送钾离子的方法
公开(公告)号：US20120083001A1
公开(公告)日：2012-04-05
申请号：US13248880
申请日：2011-09-29
申请人： Masato SUZUKI , Hiroaki Oka , Shigeki Kiyonaka , Yasuo Mori
发明人： Masato SUZUKI , Hiroaki Oka , Shigeki Kiyonaka , Yasuo Mori
IPC分类号： G01N33/566 , C12N5/071
CPC分类号： G01N33/566 , G01N33/6872
摘要： An object of the present invention is to detect a specific chemical substance with high sensitivity and high precision. By using a specific lipid bilayer membrane, a chemical substance is detected with high sensitivity and high precision. Here, the specific lipid bilayer membrane comprises a chemical substance receptor, a chimeric G protein, and a potassium ion channel. The chimeric G protein comprises a chimeric Gα subunit and a Gβγ subunit complex. The chimeric Gα subunit is selected from the group consisting of Gi/olf13 (SEQ ID NO: 04), Gi/olf28 (SEQ ID NO: 05), Gi/olf94 (SEQ ID NO: 07), Gi/olf113 (SEQ ID NO: 08), Gi/olf α3-β5,C (SEQ ID NO: 12), and Gi/olfα4-β6,C (SEQ ID NO: 15).
摘要翻译：本发明的目的是检测具有高灵敏度和高精度的特定化学物质。通过使用特定的脂质双层膜，以高灵敏度和高精度检测化学物质。这里，特定脂质双层膜包含化学物质受体，嵌合G蛋白和钾离子通道。嵌合G蛋白包含嵌合Gα亚基和G＆bgrγγ亚基复合物。嵌合Gα亚基选自Gi / olf13（SEQ ID NO：04），Gi / olf28（SEQ ID NO：05），Gi / olf94（SEQ ID NO：07），Gi / olf113（SEQ ID NO： NO：08），Gi /olfα3-和bgr; 5，C（SEQ ID NO：12）和Gi /olfα4-＆bgr; 6，C（SEQ ID NO：15）。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式