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1. 发明授权

US07055540B2 Method of moving fluid in capillary chip 有权
标题翻译：在毛细管芯片中移动流体的方法
公开(公告)号：US07055540B2
公开(公告)日：2006-06-06
申请号：US11195649
申请日：2005-08-03
申请人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
发明人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
IPC分类号： E03B1/00 , F15C1/00
CPC分类号： G01N27/44791 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0816 , B01L2300/0864 , B01L2400/0661 , F16K99/0001 , F16K99/0017 , F16K99/0036 , F16K2099/0074 , F16K2099/0084 , Y10T137/0318 , Y10T137/206 , Y10T137/2196 , Y10T137/2224
摘要： The present invention is a method of moving a fluid in a capillary using a capillary chip having a layer including a polymer composition and a capillary formed on the surface or inside of the aforementioned layer including a polymer composition, wherein the aforementioned capillary has a movement control part, and the aforementioned movement control part includes multiple and sequential opening/closing parts; the aforementioned opening/closing part blocks movement of the fluid that flows in the aforementioned capillary by increase in the volume of the aforementioned polymer composition to result in the closed state, while it permits movement of the fluid that flows in the aforementioned capillary by decrease in the volume of the aforementioned polymer composition to result in the open state; and the aforementioned method of moving the fluid includes a step (a) of switching the aforementioned multiple opening/closing parts from the open state to the closed state sequentially in a movement direction by changing the temperature of the aforementioned polymer composition in the aforementioned movement control part, thereby moving the fluid in the aforementioned capillary.
摘要翻译：本发明是一种使用毛细管芯片的毛细管芯片移动流体的方法，所述毛细管芯片具有包含聚合物组合物和形成在包括聚合物组合物的上述层的表面或内部的毛细管的毛细管，其中上述毛细管具有移动控制上述移动控制部分包括多个和顺序的打开/关闭部分; 上述打开/关闭部分通过增加上述聚合物组合物的体积来阻止在上述毛细管中流动的流体的运动，导致关闭状态，同时允许通过减少在上述毛细管中流动的流体的运动上述聚合物组合物的体积导致打开状态; 并且上述移动流体的方法包括以下步骤：（a）通过在上述移动控制中改变上述聚合物组合物的温度，将上述多个打开/关闭部分从打开状态顺序地切换到关闭状态从而使上述毛细管中的流体移动。

2. 发明申请

US20050274423A1 Method of moving fluid in capillary chip 有权
标题翻译：在毛细管芯片中移动流体的方法
公开(公告)号：US20050274423A1
公开(公告)日：2005-12-15
申请号：US11195649
申请日：2005-08-03
申请人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
发明人： Hiroaki Oka , Tetsuo Yukimasa , Maki Katagiri
IPC分类号： B01L3/00 , F15C1/04 , F15C5/00 , F16K99/00 , G01N27/447 , G01N37/00
CPC分类号： G01N27/44791 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0816 , B01L2300/0864 , B01L2400/0661 , F16K99/0001 , F16K99/0017 , F16K99/0036 , F16K2099/0074 , F16K2099/0084 , Y10T137/0318 , Y10T137/206 , Y10T137/2196 , Y10T137/2224
摘要： The present invention is a method of moving a fluid in a capillary using a capillary chip having a layer including a polymer composition and a capillary formed on the surface or inside of the aforementioned layer including a polymer composition, wherein the aforementioned capillary has a movement control part, and the aforementioned movement control part includes multiple and sequential opening/closing parts; the aforementioned opening/closing part blocks movement of the fluid that flows in the aforementioned capillary by increase in the volume of the aforementioned polymer composition to result in the closed state, while it permits movement of the fluid that flows in the aforementioned capillary by decrease in the volume of the aforementioned polymer composition to result in the open state; and the aforementioned method of moving the fluid includes a step (a) of switching the aforementioned multiple opening/closing parts from the open state to the closed state sequentially in a movement direction by changing the temperature of the aforementioned polymer composition in the aforementioned movement control part, thereby moving the fluid in the aforementioned capillary.
摘要翻译：本发明是一种使用毛细管芯片的毛细管芯片移动流体的方法，所述毛细管芯片具有包含聚合物组合物和形成在包括聚合物组合物的上述层的表面或内部的毛细管的毛细管，其中上述毛细管具有移动控制上述移动控制部分包括多个和顺序的打开/关闭部分; 上述打开/关闭部分通过增加上述聚合物组合物的体积来阻止在上述毛细管中流动的流体的运动，导致关闭状态，同时允许通过减少在上述毛细管中流动的流体的运动上述聚合物组合物的体积导致打开状态; 并且上述移动流体的方法包括：通过在上述移动控制中改变上述聚合物组合物的温度，将上述多个开闭部从打开状态向移动方向顺序地切换到闭合状态的步骤（a）从而使上述毛细管中的流体移动。

3. 发明申请

US20080293128A1 SENSOR OF PYROPHOSPHATE AND SNP TYPING SENSOR USING THE SAME 有权
标题翻译：使用相同的磷酸酯和SNP型传感器的传感器
公开(公告)号：US20080293128A1
公开(公告)日：2008-11-27
申请号：US12112249
申请日：2008-04-30
申请人： Tetsuo YUKIMASA , Hiroaki Oka
发明人： Tetsuo YUKIMASA , Hiroaki Oka
IPC分类号： C12M1/00
CPC分类号： C12Q1/6827 , C12Q2565/301
摘要： A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.
摘要翻译：提供了一种焦磷酸盐传感器，其可以在使用引物延伸反应的SNP分型中测量焦磷酸盐的方法中以高灵敏度方便地检测焦磷酸盐。焦磷酸盐传感器，其特征在于包括：绝缘基板1; 形成在其上并具有测量电极2和对电极3的电极系统; 以及设置在基板1上的多个反应试剂层，包括焦磷酸酶，甘油醛-3-磷酸脱氢酶，心肌黄酶，甘油醛-3-磷酸，氧化尼古丁胺腺嘌呤二核苷酸，电子介体，镁盐和缓冲成分，将包含缓冲成分的反应试剂层35分离出的酶的反应试剂层36和包含甘油醛-3-磷酸的反应试剂层37与包含缓冲成分的反应试剂层35分离。

4. 发明授权

US07396648B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07396648B2
公开(公告)日：2008-07-08
申请号：US11607907
申请日：2006-12-04
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , Y10T436/143333
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'端的第二个基地是T或G; 从3'的第三个基地是T或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

5. 发明授权

US07348150B1 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
公开(公告)号：US07348150B1
公开(公告)日：2008-03-25
申请号：US11633587
申请日：2006-12-05
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q2565/301
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

6. 发明申请

US20070281341A1 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US20070281341A1
公开(公告)日：2007-12-06
申请号：US11633584
申请日：2006-12-05
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6869 , C12Q2535/125
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

7. 发明授权

US07297498B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07297498B2
公开(公告)日：2007-11-20
申请号：US11488635
申请日：2006-07-19
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2535/125 , C12Q2525/185
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TA-3′, 5′-TT-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 3'末端的第2和第3个碱基为5'-AT-3'，5'-TA-3'，5'-TT-3'或5'-CT-3' 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

8. 发明授权

US07285387B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07285387B2
公开(公告)日：2007-10-23
申请号：US11363953
申请日：2006-03-01
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2535/125 , C12Q2525/185
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is A. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当靶SNP碱基的3'侧邻近的碱基为A时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基一个分隔开的基底是A.根据本发明，3'端基被设计为对应于SNP的碱基; 从3'的第二个底部到C; 3'端的第3个碱基为A或C的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

9. 发明申请

US20050158725A1 Method of amplifying nucleic acid by electromagnetic induction heating and reaction container and reaction device to be used therein 审中-公开
标题翻译：通过电磁感应加热和反应容器放大核酸的方法及其中使用的反应装置
公开(公告)号：US20050158725A1
公开(公告)日：2005-07-21
申请号：US10510819
申请日：2003-09-24
申请人： Tetsuo Yukimasa , Nobuhiko Ozaki , Masaya Nakatani , Hidenobu Yaku , Hiroaki Oka , Kimi Fukahi
发明人： Tetsuo Yukimasa , Nobuhiko Ozaki , Masaya Nakatani , Hidenobu Yaku , Hiroaki Oka , Kimi Fukahi
IPC分类号： C07H21/00 , C12Q1/68 , C12M1/34
CPC分类号： C07H21/00 , C12Q1/6844
摘要： The present invention provides a reaction container, a reaction device, and a method of amplifying a nucleic acid, which are suitable for locally heating a sample which contains a nucleic acid, enable rapid control of temperature changes in the sample, provide easy fabrication, and are low cost. The invention provides a reaction container used for a nucleic acid amplification reaction, which includes a sample holding portion formed from a cavity in the reaction container; and a heating element for heating a sample which contains a nucleic acid and which is held in the sample holding portion, wherein the heating element is made of a conductive material and arranged at a location where the sample or the sample and the periphery of the cavity is (are) to be locally heated, the conductive material generating heat by electromagnetic induction.
摘要翻译：本发明提供一种反应容器，反应装置和扩增核酸的方法，其适用于局部加热含有核酸的样品，能够快速控制样品中的温度变化，提供容易的制造，以及是低成本的。本发明提供了用于核酸扩增反应的反应容器，其包括由反应容器中的空腔形成的样品保持部分; 以及加热元件，用于加热含有核酸并被保持在样品保持部分中的样品，其中所述加热元件由导电材料制成并且布置在样品或样品和空腔周边的位置被局部加热，导电材料通过电磁感应产生热量。

10. 发明授权

US07297499B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07297499B2
公开(公告)日：2007-11-20
申请号：US11489582
申请日：2006-07-20
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2535/125 , C12Q2525/185
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T; the third base from the 3′ end to be any one of T, G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T; 3'末端的第3个碱基为T，G或C任一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

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