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11. 发明申请

US20060156434A1 Xenorhabdus TC proteins and genes for pest control 有权
公开(公告)号：US20060156434A1
公开(公告)日：2006-07-13
申请号：US11375551
申请日：2006-03-14
申请人： Scott Bintrim , Jon Mitchell , Ignacio Larrinua , Patricia Apel-Birkhold , Susan Green , Barry Schafer , Scott Bevan , Scott Young , Lining Guo
发明人： Scott Bintrim , Jon Mitchell , Ignacio Larrinua , Patricia Apel-Birkhold , Susan Green , Barry Schafer , Scott Bevan , Scott Young , Lining Guo
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04 , C07K14/195 , C07K14/415
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.

12. 发明申请

US20050130118A1 Methods for identifying inhibitors of chlorophyll synthase 审中-公开
标题翻译：鉴定叶绿素合酶抑制剂的方法
公开(公告)号：US20050130118A1
公开(公告)日：2005-06-16
申请号：US10737014
申请日：2003-12-16
申请人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
发明人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
IPC分类号： C12Q1/00 , G01N33/573
CPC分类号： G01N33/573
摘要： The present inventors have discovered that antisense suppression of a chlorophyll synthase (CS) gene results in plants exhibiting one or more of chlorosis, reduced growth, and altered development. Thus, the present inventors have discovered that the protein encoded by the CS gene is essential for normal plant growth and development, and is useful as a target for the identification of compounds as antibiotics and herbicides, especially herbicides. The present invention is directed to methods for identifying inhibitors of a CS enzyme by incubating a CS polypeptide with a chlorophyllide and a phopholipid substrate in the presence and absence of a test compound under conditions suitable for the CS enzyme activity, adding a solution to the incubation reactions comprising a water immiscible organic solvent, a water-soluble alcohol, and a water-soluble dye that absorbs in the range of one or both the excitation and emission wavelength ranges of the chlorophyllide substrate, and measuring the fluorescence of the incubation reactions at from about 650 to 750 nm, using from about 425 to 445 nm as excitation wavelength, wherein a decrease in the fluorescence in the presence of the test compound indicates that the compound is a CS inhibitor.
摘要翻译：本发明人已经发现，叶绿素合酶（CS）基因的反义抑制导致植物表现出一种或多种褪绿，生长减少和改变发育。因此，本发明人已经发现，由CS基因编码的蛋白质对于正常的植物生长和发育是必需的，并且可用作鉴定作为抗生素和除草剂，特别是除草剂的化合物的靶标。本发明涉及通过在适合于CS酶活性的条件下，在存在和不存在测试化合物的情况下，将CS多肽与叶绿素和磷脂底物一起温育来鉴定CS酶抑制剂的方法，向培养基中加入溶液包括与水不溶混的有机溶剂，水溶性醇和在所述叶绿素底物的激发和发射波长范围中的一个或两个的范围内吸收的水溶性染料的反应，以及测量从约650至750nm，使用约425至445nm作为激发波长，其中在测试化合物存在下荧光的降低表明该化合物是CS抑制剂。

13. 发明授权

US09310354B2 Methods of predicting crop yield using metabolic profiling 有权
标题翻译：使用代谢分析预测作物产量的方法
公开(公告)号：US09310354B2
公开(公告)日：2016-04-12
申请号：US14002250
申请日：2012-03-07
申请人： Kateri Duncan , Jonathan Cohn , Lining Guo , Nalini Desai , Matthew Mitchell , Jacob Wulff
发明人： Kateri Duncan , Jonathan Cohn , Lining Guo , Nalini Desai , Matthew Mitchell , Jacob Wulff
IPC分类号： G01N33/50 , A01H1/04 , G01N33/48 , G06G7/75 , A01C21/00
CPC分类号： G01N33/5097 , A01C21/007 , A01H1/04 , G01N33/48 , G01N33/5038 , G06G7/75 , Y02A40/14
摘要： The present invention relates to the fields of agriculture, plant breeding or genetic engineering for plants with increased yield, crop forecasting and crop management. In particular, the methods herein describe novel methods of predicting a plant's yield through the measurement of specific metabolites either individually or in combination with one another in a specific plant reproductive tissue. The plant predictive methods described herein may be used to predict yield of plant populations as well as allow for more efficient crop management practices (e.g. amount and timing of chemical applications or amount of irrigation water applied to a field).
摘要翻译：本发明涉及具有增加的产量，作物预测和作物管理的植物的农业，植物育种或遗传工程领域。特别地，本文的方法描述了通过在特定植物生殖组织中单独或组合地测量特定代谢物来预测植物产量的新方法。本文描述的植物预测方法可以用于预测植物种群的产量，并且允许更有效的作物管理实践（例如化学应用的量和时间或施加到田地的灌溉水的量）。

14. 发明授权

US08658351B2 Determining liver toxicity of an agent using metabolite biomarkers 有权
标题翻译：使用代谢物生物标志物测定药物的肝脏毒性
公开(公告)号：US08658351B2
公开(公告)日：2014-02-25
申请号：US13148082
申请日：2010-02-05
申请人： Michael Milburn , Lining Guo , Jacob Edward Wulff , Kay A. Lawton
发明人： Michael Milburn , Lining Guo , Jacob Edward Wulff , Kay A. Lawton
IPC分类号： C12Q1/00
CPC分类号： G01N33/5014 , G01N33/5067 , G01N33/5088
摘要： The present invention provides various biomarkers for hepatotoxicity and various methods of using the biomarkers Some of the biomarkers within the scope of this invention are cholate, glycochenodeoxycholate, glycocholate, taurine, 3-hyroxy-2-ethylpropionate, 4-imidazoleacetate, tyramine, anthranilate, 2′-deoxycytidine, N-acetyl aspartate (NAA), beta-hydroxy-hexanoate, and sarcosine (N-methylglycine) The methods of using the biomarkers include exposing a first hepatocyte culture to a test agent and comparing the levels of the one or more biomarkers obtained in the first hepatocyte culture to the levels of the one or more biomarkers obtained in a second hepatocyte culture without the test agent, where differential levels of the one or more biomarkers in the first hepatocyte culture as compared to the levels in the second hepatocyte culture is indicative of the test agent being a hepatotoxicant.
摘要翻译：本发明提供用于肝毒性的各种生物标志物和使用生物标志物的各种方法本发明范围内的一些生物标志物是胆酸，甘油脱氧胆酸，甘氨胆酸，牛磺酸，3-羟基-2-乙基丙酸酯，4-咪唑乙酸酯，酪胺，邻氨基苯甲酸酯， 2'-脱氧胞苷，N-乙酰天冬氨酸（NAA），β-羟基己酸酯和肌氨酸（N-甲基甘氨酸）使用生物标志物的方法包括将第一肝细胞培养物暴露于测试试剂，并比较其中一种或多种在第一肝细胞培养物中获得的更多生物标志物在没有测试剂的第二肝细胞培养物中获得的一种或多种生物标志物的水平，其中第一肝细胞培养物中的一种或多种生物标志物的差异水平与第二次肝细胞培养物中的水平相比肝细胞培养表明试验剂是肝毒性剂。

15. 发明申请

US20110300571A1 Determination of the Liver Toxicity of an Agent 有权
标题翻译：药物肝脏毒性的测定
公开(公告)号：US20110300571A1
公开(公告)日：2011-12-08
申请号：US13148082
申请日：2010-02-05
申请人： Michael Milburn , Lining Guo , Jacob Edward Wulff , Kay A. Lawton
发明人： Michael Milburn , Lining Guo , Jacob Edward Wulff , Kay A. Lawton
IPC分类号： C12Q1/02 , G01N27/00
CPC分类号： G01N33/5014 , G01N33/5067 , G01N33/5088
摘要： The present invention provides various biomarkers for hepatotoxicity and various methods of using the biomarkers Some of the biomarkers within the scope of this invention are cholate, glycochenodeoxycholate, glycocholate, taurine, 3-hyroxy-2-ethylpropionate, 4-imidazoleacetate, tyramine, anthranilate, 2′-deoxycytidine, N-acetyl aspartate (NAA), beta-hydroxy-hexanoate, and sarcosine (N-methylglycine) The methods of using the biomarkers include exposing a first hepatocyte culture to a test agent and comparing the levels of the one or more biomarkers obtained in the first hepatocyte culture to the levels of the one or more biomarkers obtained in a second hepatocyte culture without the test agent, where differential levels of the one or more biomarkers in the first hepatocyte culture as compared to the levels in the second hepatocyte culture is indicative of the test agent being a hepatotoxicant.
摘要翻译：本发明提供用于肝毒性的各种生物标志物和使用生物标志物的各种方法本发明范围内的一些生物标志物是胆酸，甘油脱氧胆酸，甘氨胆酸，牛磺酸，3-羟基-2-乙基丙酸酯，4-咪唑乙酸酯，酪胺，邻氨基苯甲酸酯， 2'-脱氧胞苷，N-乙酰天冬氨酸（NAA），β-羟基己酸酯和肌氨酸（N-甲基甘氨酸）使用生物标志物的方法包括将第一肝细胞培养物暴露于测试试剂，并比较其中一种或多种在第一肝细胞培养物中获得的更多生物标志物在没有测试剂的第二肝细胞培养物中获得的一种或多种生物标志物的水平，其中第一肝细胞培养物中的一种或多种生物标志物的差异水平与第二次肝细胞培养物中的水平相比肝细胞培养表明试验剂是肝毒性剂。

16. 发明授权

US06770452B1 Methods for the identification of inhibitors of serine acetyltransferase activity in plants 失效
标题翻译：鉴定植物中丝氨酸乙酰转移酶活性抑制剂的方法
公开(公告)号：US06770452B1
公开(公告)日：2004-08-03
申请号：US10402818
申请日：2003-03-28
申请人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
发明人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
IPC分类号： C12Q148
CPC分类号： C12N9/1029 , C12N15/8261 , C12N15/8274 , C12Q1/48 , G01N2333/91057 , G01N2500/02 , Y02A40/146
摘要： The present inventors have discovered that serine acetyltransferase (SAT) is essential for plant growth. Specifically, the inhibition of SAT gene expression in plant seedlings results in reduced growth and altered pigmentation. Thus, SAT is useful as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of herbicides by measuring the activity of an SAT in the presence and absence of a compound, wherein an alteration of SAT activity in the presence of the compound indicates the compound as a candidate for a herbicide.
摘要翻译：本发明人发现丝氨酸乙酰转移酶（SAT）对于植物生长是必需的。具体地说，在植物幼苗中抑制SAT基因表达导致生长减少和色素沉着改变。因此，SAT可用作鉴定除草剂的靶标。因此，本发明提供了通过在化合物存在和不存在下测量SAT的活性来鉴定除草剂的方法，其中在化合物存在下SAT活性的改变表示作为除草剂候选物的化合物。

17. 发明申请

US20140189903A1 METHODS OF PREDICTING YIELD IN PLANTS AND APPLICATIONS OF USE THEREOF 有权
标题翻译：在植物中预测产量的方法及其应用
公开(公告)号：US20140189903A1
公开(公告)日：2014-07-03
申请号：US14002250
申请日：2012-03-07
申请人： Kateri Duncan , Jonathan Cohn , Lining Guo , Nalini Desai , Matthew Mitchell , Jacob Wulff
发明人： Kateri Duncan , Jonathan Cohn , Lining Guo , Nalini Desai , Matthew Mitchell , Jacob Wulff
IPC分类号： G01N33/50
CPC分类号： G01N33/5097 , A01C21/007 , A01H1/04 , G01N33/48 , G01N33/5038 , G06G7/75 , Y02A40/14
摘要： The present invention relates to the fields of agriculture, plant breeding or genetic engineering for plants with increased yield, crop forecasting and crop management. In particular, the methods herein describe novel methods of predicting a plant's yield through the measurement of specific metabolites either individually or in combination with one another in a specific plant reproductive tissue. The plant predictive methods described herein may be used to predict yield of plant populations as well as allow for more efficient crop management practices (e.g. amount and timing of chemical applications or amount of irrigation water applied to a field).
摘要翻译：本发明涉及具有增加的产量，作物预测和作物管理的植物的农业，植物育种或遗传工程领域。特别地，本文的方法描述了通过在特定植物生殖组织中单独或组合地测量特定代谢物来预测植物产量的新方法。本文描述的植物预测方法可以用于预测植物种群的产量，并且允许更有效的作物管理实践（例如化学应用的量和时间或施加到田地的灌溉水的量）。

18. 发明申请

US20110030099A1 PLANTS AND MODULATORS FOR IMPROVED DROUGHT TOLERANCE 审中-公开
标题翻译：植物和调节剂改善耐旱性
公开(公告)号：US20110030099A1
公开(公告)日：2011-02-03
申请号：US12837905
申请日：2010-07-16
申请人： John Andrew Ryals , Lining Guo , Kay Ann Lawton , Xi Chen
发明人： John Andrew Ryals , Lining Guo , Kay Ann Lawton , Xi Chen
IPC分类号： A01H1/06 , A01H5/00
CPC分类号： C12N15/8218 , C12N15/8273
摘要： Methods of conferring increased drought tolerance to a plant by reducing or inhibiting ammonia accumulation, and plants produced thereby.
摘要翻译：通过减少或抑制氨积累赋予植物耐旱性增加的方法，以及由此产生的植物。

19. 发明申请

US20050074829A1 Methods for identifying inhibitors of sterol 14-alpha-demethylase 审中-公开
标题翻译：确定甾醇14-α-脱甲基酶抑制剂的方法
公开(公告)号：US20050074829A1
公开(公告)日：2005-04-07
申请号：US10678571
申请日：2003-10-03
申请人： Abubakr Aslamkhan , Lining Guo , John Rice
发明人： Abubakr Aslamkhan , Lining Guo , John Rice
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/48
CPC分类号： C12Q1/26 , C12Q1/32 , G01N2500/00
摘要： In the methods of the present invention, the change in absorbance of an obtusifoliol 14α-demethylase (OBT-DM) enzyme upon binding of an inhibitor is used to advantage for the identification of inhibitors of OBT-DM activity. Rather than measuring the absorbance of an OBT-DM/inhibitor complex over a spectrum of wavelengths as described previously, the present invention discloses methods for identifying type II inhibitors of OBT-DM by monitoring the absorbance only at 413 nm and 432 nm. The methods of the invention enable the concurrent testing of multiple compounds using a high throughput format such as with 96- or 384-well plates. The OBT-DM polypeptides of the invention include plant, fungal and human OBT-DM polypeptides, and in particular, Arabidopsis thaliana OBT-DM polypeptide
摘要翻译：在本发明的方法中，使用抑制剂结合时，obtusifoliol14α-去甲基化酶（OBT-DM）的吸光度变化有利于鉴定OBT-DM活性的抑制剂。本发明不是如前所述测量波长范围内的OBT-DM /抑制剂复合物的吸光度，而是通过仅在413nm和432nm监测吸光度来公开鉴定OBT-DM的II型抑制剂的方法。本发明的方法使得能够使用高通量形式（例如96孔或384孔板）同时测试多种化合物。本发明的OBT-DM多肽包括植物，真菌和人类OBT-DM多肽，特别是拟南芥OBT-DM多肽

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