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1. 发明授权

US5616498A Biological removal of dihalocarboxylic acid irritants 失效
标题翻译：生物去除二卤羧酸刺激物
公开(公告)号：US5616498A
公开(公告)日：1997-04-01
申请号：US508171
申请日：1995-07-27
申请人： Olivier Favre-Bulle , Jean-Marc Ricca
发明人： Olivier Favre-Bulle , Jean-Marc Ricca
IPC分类号： C12P13/04 , C07C227/40 , C07C231/22 , C12S99/00 , D06M16/00
CPC分类号： C12R1/01 , C07C227/40 , C07C231/22
摘要： Contaminating trace amounts of byproduct dihalocarboxylic acids or salts thereof are removed from aqueous solutions which comprise less than 1,000 ppm of same and at least 20% by weight of amino acids or amino acid derivatives, to render such aqueous solutions nonirritating to biological membranes and suitable for cosmeticology applications, by directly contacting the original aqueous solutions with either (a) about 1 to 100 ppm of a microorganism producing an enzyme specific for the decomposition of said dihalocarboxylic acids or salts thereof, or (b) about 0.1 to 10 ppm of such enzyme, per se.
摘要翻译：污染痕量的副产物二卤羧酸或其盐从包含小于1,000ppm相同和至少20重量％的氨基酸或氨基酸衍生物的水溶液中除去，以使这种水溶液不刺激生物膜并适于通过直接使原始水溶液与（a）约1至100ppm的微生物直接接触，所述微生物产生对所述二卤代羧酸或其盐的分解特异性的酶，或（b）约0.1至10ppm的这种酶本身。

2. 发明授权

US07247462B2 Coated enzyme-containing catalyst 有权
标题翻译：包被的含酶催化剂
公开(公告)号：US07247462B2
公开(公告)日：2007-07-24
申请号：US10311951
申请日：2001-06-22
申请人： Olivier Favre-Bulle , Jean-Claude Le Thiesse
发明人： Olivier Favre-Bulle , Jean-Claude Le Thiesse
IPC分类号： C12P7/62 , C12N11/14 , C12N11/04 , C12N9/98 , C12N9/96 , C12N1/20 , C12N15/00
CPC分类号： C12N11/08
摘要： An enzymatic catalyst in the granular form which is non-dispersible in water comprising an inner core provided with a coating which coating comprises an enzymatic material characterised in that the coating has a thickness of from 30 to 400 microns and the ratio of the size of the core to the thickness of the coating is from 2 to 20. A method for the preparation of the catalyst and the use of the catalyst in the conversion of 2-hydroxy-4-methylthio butyronitrile to ammonium 2-hydroxy-4-methylthio butanoate is also claimed.
摘要翻译：颗粒形式的酶催化剂，其不可分散在水中，其包含提供涂层的内芯，该涂层包含酶材料，其特征在于所述涂层具有30至400微米的厚度和核心至涂层的厚度为2至20.制备催化剂的方法和使用该催化剂将2-羟基-4-甲硫基丁腈转化成2-羟基-4-甲硫基丁酸铵为也声称。

3. 发明授权

US06214592B1 Enzymes and microorganisms having amidase activity for hydrolysing polyamides 失效
标题翻译：具有用于水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06214592B1
公开(公告)日：2001-04-10
申请号：US09000064
申请日：1998-04-03
申请人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
发明人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
IPC分类号： C12P1302
CPC分类号： C12R1/15 , C12N9/80
摘要： The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.6 (formula I) and/or PA 6 (formula II), said enzyme being characterized by a ratio Ra = ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ ⁢ of ⁢ ⁢ formula ⁢ ⁢ ⁢ ( I ) ⁢ ⁢ ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ of ⁢ ⁢ ⁢ formul ⁢ ⁢ a ⁢ ⁢ ( ⁢ II) ⁢ = E . ⁢ A . ⁢ ( I ) E . ⁢ A . ⁢ ( II ) which is greater than 2, preferably greater than or equal to 10 and particularly preferably greater than or equal to 50. More precisely, this enzyme consists of the peptide sequence corresponding to the attached sequence SEQ ID NO: 2. The invention further relates to the DNA which codes for this polypeptide SEQ ID NO: 2 and whose sequence corresponds to the attached sequence SEQ ID NO: 1. The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及具有酰胺酶活性的酶，特别是涉及衍生自PA 6.6（式I）和/或PA 6（式II）的低聚物类型的底物，所述酶的特征在于大于2，优选更大更优选地，该酶由对应于所附连接序列SEQ ID NO：2的肽序列组成。本发明还涉及编码该多肽SEQ ID NO： NO：2，其序列对应于所附序列SEQ ID NO：1。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

4. 发明授权

US07595181B2 Thermostable esterases from thermophilic bacteria 失效
标题翻译：嗜热细菌的热稳定酯酶
公开(公告)号：US07595181B2
公开(公告)日：2009-09-29
申请号：US10954826
申请日：2004-09-30
申请人： Burghard Grüning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
发明人： Burghard Grüning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
IPC分类号： C12N9/18 , C07K14/195 , C12P21/02 , C12P7/64
CPC分类号： C12N9/14
摘要： The present invention relates to a newly identified hydrolase from thermophilic microorganisms having thermostable properties, and more specifically, to a novel thermostable hydrolase showing high activity at high temperatures.
摘要翻译：本发明涉及具有热稳定性质的新鉴定的嗜热微生物水解酶，更具体地说，涉及一种在高温下显示高活性的新型热稳定水解酶。

5. 发明授权

US06180388B2 Enzymes and micro organisms with amidase activity which hydrolyze polyamides 失效
标题翻译：具有水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06180388B2
公开(公告)日：2001-01-30
申请号：US09000040
申请日：1998-04-03
申请人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
发明人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
IPC分类号： C12N980
CPC分类号： C12R1/01 , C12N9/80
摘要： The present invention relates to a process for the enzymatic hydrolysis of polyamides 6.6 to give adipic acid monomers and hexamethylenediamine monomers. The present invention further relates to an enzyme with amidase activity particularly towards substrates of the oligomer type derived from PA 6.6 and/or PA 6, said enzyme being characterized in that it consists of a peptide sequence corresponding to SEQ ID NO: 1 in the attached sequence listing and/or at least one polypeptide homologous to this sequence. The invention further relates to the DNA coding for said enzyme and to the biological precursors thereof The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及聚酰胺6.6的酶水解以得到己二酸单体和六亚甲基二胺单体的方法。本发明还涉及具有酰胺酶活性的酶，特别是衍生自PA 6.6和/或PA 6的低聚物类型的底物，所述酶的特征在于其由相应于所附载体中SEQ ID NO：1的肽序列组成序列表和/或与该序列同源的至少一种多肽。本发明还涉及编码所述酶及其生物前体的DNA。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

6. 发明授权

US06180359B2 Industrial scale process for the preparation of 2-hydroxy-4-methylbutyric acid using a nitrilase 失效
标题翻译：使用腈水解酶制备2-羟基-4-甲基丁酸的工业规模工艺
公开(公告)号：US06180359B2
公开(公告)日：2001-01-30
申请号：US08957621
申请日：1997-10-24
申请人： Olivier Favre-Bulle , J{acute over (e)}r{circumflex over (o)}me Pierrard , Christophe David , Philippe Morel , Dominique Horbez
发明人： Olivier Favre-Bulle , J{acute over (e)}r{circumflex over (o)}me Pierrard , Christophe David , Philippe Morel , Dominique Horbez
IPC分类号： C12P100
CPC分类号： C12P11/00 , C12N9/78 , C12N11/08
摘要： This invention relates to a process for the preparation of 2-hydroxy-4-methylthiobutyric acid or the ammonium salt of 2-hydroxy-4-methylthiobutyric acid by enzymatic hydrolysis of 2-hydroxy-4-methylthiobutyronitrile, comprising: a) preparing a biological material having a nitrilase activity; b) immobilizing the biological material, c) exposing the 2-hydroxy-4-methylthiobutyronitrile to the biological material thus immobilized to obtain the ammonium salt of 2-hydroxy-4-methylthiobutyric acid; and d) optionally converting the salt obtained to the corresponding acid.
摘要翻译：本发明涉及通过2-羟基-4-甲硫基丁腈的酶水解制备2-羟基-4-甲硫基丁酸或2-羟基-4-甲硫基丁酸铵盐的方法，包括：a）制备生物具有腈水解酶活性的材料; b）固定生物材料，c）将2-羟基-4-甲基硫代丁腈暴露于由此固定的生物材料中，得到2-羟基-4-甲硫基丁酸的铵盐; 和d）任选地将获得的盐转化成相应的酸。

7. 发明授权

US07361507B1 Method for modulating nitrilase selectivity, nitrilases obtained by said method and use thereof 失效
标题翻译：调节腈水解酶选择性的方法，通过所述方法获得的腈水解酶及其用途
公开(公告)号：US07361507B1
公开(公告)日：2008-04-22
申请号：US10129560
申请日：2000-10-30
申请人： Jérôme Pierrard , Olivier Favre-Bulle , Catherine Jourdat
发明人： Jérôme Pierrard , Olivier Favre-Bulle , Catherine Jourdat
IPC分类号： C12N15/00 , C12Q1/34 , C12N9/78 , C12P21/06 , G01N33/00 , C07K14/00 , C07H21/04
CPC分类号： C12N9/78 , Y10T436/143333
摘要： The invention concerns a modified nitrilase with modulated selectivity, characterized in that it comprises in position 162 an amino acid residue different from the original amino acid residue.
摘要翻译：本发明涉及一种具有调节选择性的修饰的腈水解酶，其特征在于其在位置162处包含不同于原始氨基酸残基的氨基酸残基。

8. 发明申请

US20060008887A1 Production of carboxylic acid and carbonic acid derivatives using a thermostable esterase 审中-公开
标题翻译：使用热稳定酯酶生产羧酸和碳酸衍生物
公开(公告)号：US20060008887A1
公开(公告)日：2006-01-12
申请号：US10955053
申请日：2004-09-30
申请人： Burghard Gruning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
发明人： Burghard Gruning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
IPC分类号： C12P7/64
CPC分类号： C12P7/6418 , C12P7/42 , C12P7/62 , C12P7/625 , C12P7/6436 , C12P9/00 , C12P11/00 , C12P13/02 , C12P17/10 , C12P17/12
摘要： The present invention relates to processes for the production of acyl compounds using an esterase having thermostable properties, and to products of such processes.
摘要翻译：本发明涉及使用具有热稳定性的酯酶制备酰基化合物的方法以及这些方法的产物。

9. 发明授权

US06417395B1 Process for preparing methionine 有权
标题翻译：制备甲硫氨酸的方法
公开(公告)号：US06417395B1
公开(公告)日：2002-07-09
申请号：US09830987
申请日：2001-07-12
申请人： Hervé Ponceblanc , Olivier Favre-Bulle , Georges Gros
发明人： Hervé Ponceblanc , Olivier Favre-Bulle , Georges Gros
IPC分类号： C07C32100
CPC分类号： C12P13/02 , C07C319/20 , C07C323/58 , C07C323/60
摘要： The invention concerns a method for preparing methionine aminoamide. The invention also concerns the preparation of methionine without co-production of salts, from an aqueous solution containing essentially methionine aminonitrile by producing during a first step aminoamide followed by two complementary steps.
摘要翻译：本发明涉及一种制备甲硫氨酸氨基酰胺的方法。本发明还涉及通过在第一步氨基酰胺中产生氨基酰胺，然后通过两个互补步骤，从含有基本上甲硫氨酸氨基腈的水溶液中共同制备甲硫氨酸。

10. 发明申请

US20050112644A1 Thermostable hydrolase 失效
标题翻译：热稳定水解酶
公开(公告)号：US20050112644A1
公开(公告)日：2005-05-26
申请号：US10954826
申请日：2004-09-30
申请人： Burghard Gruning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
发明人： Burghard Gruning , Geoffrey Hills , Thomas Veit , Christian Weitemeyer , Olivier Favre-Bulle , Fabrice Lefevre , Hong-Khanh Nguyen , Gilles Ravot
IPC分类号： C12N9/14 , C12Q1/68 , C07H21/04 , C12N9/16 , C12N9/24 , C12N9/48 , C12P7/40 , C12P7/62
CPC分类号： C12N9/14
摘要： The present invention relates to a newly identified hydrolase from thermophilic microorganisms having thermostable properties, and more specifically, to a novel thermostable hydrolase showing high activity at high temperatures.
摘要翻译：本发明涉及具有热稳定性质的新鉴定的嗜热微生物水解酶，更具体地说，涉及一种在高温下显示高活性的新型热稳定水解酶。

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