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1. 发明申请

WO1998026048A1 METHODS FOR PURIFYING VIRUSES 审中-公开
标题翻译：净化病毒的方法
公开(公告)号：WO1998026048A1
公开(公告)日：1998-06-18
申请号：PCT/US1997022134
申请日：1997-12-11
申请人： SCHERING CORPORATION
发明人： SCHERING CORPORATION , TANG, John, Chu-Tay , VELLEKAMP, Gary, J. , BONDOC, Laureano, L., Jr.
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , C12N2710/10051
摘要： The invention provides methods for purifying a virus from impurities in an aqueous medium.
摘要翻译：本发明提供了从水性介质中的杂质中纯化病毒的方法。

2. 发明申请

WO1992008787A1 METHOD OF PROPAGATING HUMAN PARAMYXOVIRUSES USING CONTINUOUS CELL LINES 审中-公开
标题翻译：使用连续细胞系传播人参虫病毒的方法
公开(公告)号：WO1992008787A1
公开(公告)日：1992-05-29
申请号：PCT/US1991008446
申请日：1991-11-12
申请人： THE UNITED STATES OF AMERICA, represented by THE ...
发明人： THE UNITED STATES OF AMERICA, represented by THE ... , HIERHOLZER, John, C.
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , C12N2760/18051
摘要： A method of propagating human paramyxoviruses using a continuous cell line such as NCI-H292 (Hut-292), provides a suitable alternative to MK cells for the isolation and propagation of the human paramyxoviruses.

3. 发明申请

WO1989008701A1 PROCESS AND DEVICE FOR PRODUCING VIRUSES AND VIRAL ANTIGENS 审中-公开
标题翻译：用于生产病毒和病毒抗原的方法和装置
公开(公告)号：WO1989008701A1
公开(公告)日：1989-09-21
申请号：PCT/EP1989000245
申请日：1989-03-09
申请人： INSTITUT FÜR ANGEWANDTE BIOTECHNOLOGIE DER TROPEN , SEIFERT, Horst, S., H. , BÖHNEL, Helge , ROTH, Frauke
发明人： INSTITUT FÜR ANGEWANDTE BIOTECHNOLOGIE DER TROPEN
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , C12M23/58 , C12M47/10 , C12N2710/00051
摘要： Viruses or viral antigens are obtained by culturing animal cells in a nutrient medium suspension. The animal cells are cultured in a first bioreactor (A) under continuous addition of nutrient medium in suspension. When a given cell concentration is reached, the cell suspension is transferred to a second bioreactor (B) where it is used as the nutrient medium for culturing the virus under continuous addition of a conservation medium. When a given quantity of virus is produced, the cells and cell residues are continuously separated from the virus-containing nutrient medium suspension in a first filtration arrangement and the residue is discarded. The virus or viral antigen and nutrient medium suspension in the filtrate are continuously separated in a second filtration arrangement. The virus or viral antigen are concentrated and the nutrient medium is discarded.

4. 发明申请

WO1997011160A1 METHOD OF ADSORBING VIRUSES FROM FLUID COMPOSITIONS 审中-公开
标题翻译：从流体组合物中吸入病毒的方法
公开(公告)号：WO1997011160A1
公开(公告)日：1997-03-27
申请号：PCT/US1996015500
申请日：1996-09-17
申请人： LIGOCHEM, INC. , KRUPEY, John , SMITH, Allen, D. , ARNOLD, Edward , DONNELLY, Robert
发明人： LIGOCHEM, INC.
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , C08F8/14 , C08F2810/50 , C12N2710/10351 , C12N2795/10343 , C12N2795/14143 , C08F212/08 , C08F222/06 , C08F222/08
摘要： A method of adsorbing from a solution comprising a biological sample viruses which retain their viability and infectivity. The method comprises adjusting the pH of said solution to pH 6.0 to 8.0; adding an effective amount of a water insoluble cross-linked polycarboxylic acid polymer ("WCPP") into said solution in a volume:volume ratio of WCPP to solution of 100:1 to 1:10,000 to form a WCPP-solution mixture; incubating said WCPP-solution mixture for a time sufficient to immobilize said viruses on said WCPP forming a WCPP-virus matrix; and separating said matrix from said solution. This novel method is suitable for removing, purifying, recovering and analyzing viable viruses as well as viral components such as viral proteins and nucleic acids.
摘要翻译：从包含保留其活力和感染性的生物样品病毒的溶液中吸附的方法。该方法包括将所述溶液的pH调节至pH 6.0至8.0; 将WCPP与溶液的体积比为100:1-1:10,000添加到所述溶液中的有效量的水不溶性交联聚羧酸聚合物（“WCPP”）以形成WCPP溶液混合物; 将所述WCPP-溶液混合物温育足以将所述病毒固定在形成WCPP病毒基质的所述WCPP上的时间; 并从所述溶液中分离所述基质。该新方法适用于去除，纯化，回收和分析活病毒以及病毒组分如病毒蛋白和核酸。

5. 发明申请

WO1997006243A1 METHOD FOR PURIFYING VIRUSES BY CHROMATOGRAPHY 审中-公开
标题翻译：通过色谱法净化病毒的方法
公开(公告)号：WO1997006243A1
公开(公告)日：1997-02-20
申请号：PCT/FR1996001064
申请日：1996-07-08
申请人： PASTEUR MERIEUX SERUMS ET VACCINS , FANGET, Bernard , FRANÇON, Alain
发明人： PASTEUR MERIEUX SERUMS ET VACCINS
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , A61K39/12 , A61K39/205 , C07H15/04 , C12N9/80 , C12N2760/20134 , C12N2760/20151 , C12P13/001 , C12P13/02 , C12P19/26 , Y02A50/39
摘要： A method for purifying viruses from a cell line culture by chromatography, comprising an anion exchange chromatography step followed by a cation exchange chromatography step and optionally a metal-binding affinity chromatography step. The method is particularly suitable for producing viruses for use in vaccines.
摘要翻译：一种通过色谱从细胞系培养物中纯化病毒的方法，包括阴离子交换层析步骤，接着是阳离子交换层析步骤和任选的金属结合亲和层析步骤。该方法特别适用于生产用于疫苗的病毒。

6. 发明申请

WO1995002688A1 METHODS AND COMPOSITIONS FOR THE REMOVAL OF VIRUSES AND USES THEREOF 审中-公开
标题翻译：用于去除病毒的方法和组合物及其用途
公开(公告)号：WO1995002688A1
公开(公告)日：1995-01-26
申请号：PCT/US1994007783
申请日：1994-07-15
申请人： VIAGENE, INC.
发明人： VIAGENE, INC. , PRUSSAK, Charles, E.
IPC分类号： C12N07/02
CPC分类号： C07K14/005 , C12N7/00 , C12N2740/13022 , C12N2740/13051
摘要： Methods and compositions useful for the separation of viruses, including retroviruses and viral vectors, from preparations are disclosed. Sulfated oligosaccharides with at least about 6 mu moles sulfate per gram of oligosaccharide are provided. In one aspect, a sulfated oligosaccharide of the present invention may be used in the purification of a virus, such as a lipid envelope virus. The present invention also discloses methods for the removal of a contaminating virus from a preparation, such as biologic therapeutics.
摘要翻译：公开了用于从制剂中分离病毒（包括逆转录病毒和病毒载体）的方法和组合物。提供了每克寡糖至少约6微摩尔硫酸盐的硫酸化低聚糖。一方面，本发明的硫酸化寡糖可以用于病毒如脂质包膜病毒的纯化。本发明还公开了从制剂如生物治疗剂中除去污染性病毒的方法。

7. 发明申请

WO1994028115A1 DIRECT AND BIOCHEMICALLY FUNCTIONAL DETECTION PROCESS OF RETROVIRUS IN BIOLOGICAL SAMPLES 审中-公开
标题翻译：方法直接和生化功能检测逆转录病毒的生物样品
公开(公告)号：WO1994028115A1
公开(公告)日：1994-12-08
申请号：PCT/DE1994000610
申请日：1994-05-31
申请人： FAFF, Ortwin
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , C12N2740/10051 , C12Q1/48 , C12Q1/702 , C12Q1/703 , Y10S435/961 , Y10S435/968 , Y10S435/974
摘要： A routine, direct and biochemically functional process is disclosed for detecting retrovirus in biological samples. For that purpose, structure-specific extraction with immobilised ligands directed against the viral surface is combined with function-specific enzyme reaction for retrovirus by means of reverse transcriptase, RNAse H, integrase or protease. Reverse transcription by means of reverse transcriptase, retroviral RNA and tRNA is particularly suitable for the retrovirus-specific enzyme reaction. In an ulterior detection step, the newly synthesised cDNA products are identified by radioactive, photometric, luminescent or fluorescent processes. This process represents a biochemical simulation of the natural process of retroviral infection and development within the cell (reverse transcription, integration, maturation) and allows several retroviral components, surface glycoproteins, enzymes, structural proteins, RNA and tRNA to be simultaneously detected. A process with a qualitatively improved specificity compared with state-of-the-art processes is thus obtained. The process takes 1-2 days, may be easily and routinely carried out in a microtiter format and thus allows many probes to be simultaneously processed within a short time. In addition, the process may be universally applied to all species of retrovirus by means of corresponding specific ligands. The process is suitable for detecting retrovirus for the purpose of diagnosing, following and treating viral diseases, in transfusion and transplantation medicine, in virological research and development and for the biological quality control of pharmaceutical and biotechnological products.
摘要翻译：所描述的方法允许生物样品中的逆转录病毒的常规直接和生物化学功能周志武检测。这是通过一个特定的结构提取与固定针对病毒表面配体与逆转录酶，RNA酶H，整合酶，或蛋白酶的反转录病毒的装置的后续功能特定的酶反应相结合来实现。对于逆转录病毒特异性酶反应，特别是反转录，在逆转录酶，逆转录病毒RNA和tRNA参与。在随后的检测步骤中，新合成的cDNA产物通过放射性，光度，发光或荧光的方法鉴定。此方法是生化模仿在细胞中逆转录病毒感染和其处理的自然过程（逆转录，集成，成熟）和允许几个逆转录病毒组分的同时检测等的表面糖蛋白，酶，结构蛋白，RNA和tRNA。这也提供了比以前的方法，该方法的质量更好的特异性保证。该过程需要约1-2天可在微量滴定格式容易且常规地进行，使许多样品的同时处理在短时间内。此外，使用相应的特异性配体的方法是普遍适用于逆转录病毒的所有物种。该方法适用于诊断，当然和病毒疾病的治疗的逆转录病毒检测在其中输血和移植医学，在病毒学研究和开发以及医药和生物技术产品的生物质量控制。

8. 发明申请

WO1998000524A1 METHOD FOR PRODUCING RECOMBINANT ADENOVIRUS 审中-公开
标题翻译：生产重组腺病毒的方法
公开(公告)号：WO1998000524A1
公开(公告)日：1998-01-08
申请号：PCT/FR1997001107
申请日：1997-06-20
申请人： RHONE-POULENC RORER S.A. , BLANCHE, Francis , GUILLAUME, Jean-Marc
发明人： RHONE-POULENC RORER S.A.
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , B01D15/363 , B01D61/145 , B01J41/20 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , G01N30/02
摘要： The invention concerns a method for producing recombinant adenovirus by which viral DNA is introduced in a packaging cell culture and the viruses produced are harvested after liberation in the supernatant. The invention also concerns the viruses produced and their use.
摘要翻译：本发明涉及生产重组腺病毒的方法，通过该方法将病毒DNA引入包装细胞培养物中，并且在上清液中释放后收获产生的病毒。本发明还涉及所产生的病毒及其用途。

9. 发明申请

WO1997032010A1 METHOD OF PURIFYING AND REMOVING VIRUSES 审中-公开
标题翻译：净化和移除病毒的方法
公开(公告)号：WO1997032010A1
公开(公告)日：1997-09-04
申请号：PCT/JP1997000457
申请日：1997-02-19
申请人： TAKARA SHUZO CO., LTD. , UENO, Takashi , HASHINO, Kimikazu , ASADA, Kiyozo , KATO, Ikunoshin
发明人： TAKARA SHUZO CO., LTD.
IPC分类号： C12N07/02
CPC分类号： C12N7/00 , A61L2/0011 , A61L2/0082 , C12N2740/13051 , C12P19/04
摘要： A method of purifying or removing viruses contained in samples, characterized by comprising the step of adsorbing the viruses on a fucose sulfate-containing polysaccharide or a degradation product thereof.
摘要翻译：一种纯化或除去样品中所含病毒的方法，其特征在于包括将病毒吸附在含岩藻糖硫酸盐的多糖或其降解产物上的步骤。

10. 发明申请

WO1994002596A1 METHOD FOR PREVENTING ZOSTER OR ALLEVIATING VARICELLA RELATED POST-HERPETIC NEURALGIA 审中-公开
标题翻译：预防痉挛性视网膜病变相关后遗传神经病的方法
公开(公告)号：WO1994002596A1
公开(公告)日：1994-02-03
申请号：PCT/US1993006581
申请日：1993-07-13
申请人： MERCK & CO., INC.
发明人： MERCK & CO., INC. , CALANDRA, Gary, B. , PROVOST, Philip, J. , LEVIN, Myron, J. , WHITE, C., Jo
IPC分类号： C12N07/02
CPC分类号： A61K39/25 , A61K39/12 , A61K2039/5254 , C12N2710/16734
摘要： Herpes Zoster, or varicella related post herpetic neuralgia is alleviated by immunizing people at risk of developing herpes zoster with varicella zoster virus (VZV) antigen.
摘要翻译：带状疱疹带状疱疹或水痘相关的疱疹性神经痛可以通过免疫接种水痘带状疱疹病毒（VZV）抗原的带状疱疹风险的人来缓解。

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