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1. 发明申请

WO1994006821A1 A PROCESS FOR THE PURIFICATION OF AQUEOUS EXTRACTS CONTAINING ALLERGENICALLY ACTIVE PROTEINS, EXTRACTS OBTAINABLE ACCORDING TO THIS PROCESS AS WELL AS THEIR USE 审中-公开
标题翻译：含有活性蛋白质的水性提取物的纯化方法，根据本方法可获得的提取物
公开(公告)号：WO1994006821A1
公开(公告)日：1994-03-31
申请号：PCT/NL1992000160
申请日：1992-09-21
申请人： LABORATORIOS LETI S.A. , BERRENS, Lubertus
发明人： LABORATORIOS LETI S.A.
IPC分类号： C07K03/12
CPC分类号： A61K39/35 , A61K39/36
摘要： The invention concerns the removal of various allergologically irrelevant low-molecular weight components from the usual aqueous extracts of allergenically active proteins of plant pollens. Described are the desorption and subsequent elimination, from traditionally prepared allergenic pollen protein extracts, of low-molecular weight pigment and other compounds which are normally retained by strong electrostatic and/or hydrophobic forces. The preparation of such depigmented pollen proteins does not impair their allergenic potency or immunological specificity. The invention enables the production of fully active allergenic pollen proteins devoid of adhering low-molecular weight substances interfering with their safety, diagnostic accuracy and clinical efficacy. The purified pollen proteins represent improved starting materials for chemical derivatization, i.e., the preparation of attenuated vaccines for immunotherapy. The invention also provides suitable products for investigating the molecular structure of allergenic epitopes.
摘要翻译：本发明涉及通过植物花粉过敏性活性蛋白质的通常含水提取物去除各种变应原不相关的低分子量组分。描述了从传统制备的过敏花粉蛋白提取物中解吸和随后的消除低分子量颜料和通常被强烈的静电和/或疏水力保持的其它化合物。这种脱色花粉蛋白的制备不会损害其过敏效力或免疫特异性。本发明能够生产没有粘附低分子量物质的完全活性的过敏性花粉蛋白质干扰其安全性，诊断准确性和临床疗效。纯化的花粉蛋白代表用于化学衍生化的改进起始物质，即用于免疫治疗的减毒疫苗的制备。本发明还提供了适用于研究过敏原表位的分子结构的产物。

2. 发明申请

WO1991017178A1 METHOD FOR PURIFYING RECOMBINANTLY-PRODUCED PROTEINS 审中-公开
标题翻译：净化重组蛋白的方法
公开(公告)号：WO1991017178A1
公开(公告)日：1991-11-14
申请号：PCT/US1991003152
申请日：1991-05-07
申请人： RORER INTERNATIONAL (HOLDINGS), INC. , DARLING, Thomas, L., J. , AKHNANA, Lida, Y. , MITSCHELEN, Jonathan, J. , HRINDA, Michael, E.
发明人： RORER INTERNATIONAL (HOLDINGS), INC.
IPC分类号： C07K03/12
CPC分类号： C07K14/755
摘要： This invention provides a method for the recovery of recombinantly produced proteins using mechanical disruption of the inclusion bodies in the method. Specifically, the invention provides a method for recovering vWF fragment from inclusion bodies of transformed E. coli.
摘要翻译：本发明提供了一种在该方法中机械破坏包涵体的方法来回收重组产生的蛋白质。具体地说，本发明提供从转化大肠杆菌的包涵体中回收vWF片段的方法。

3. 发明申请

WO1991000290A1 METHOD OF CLEANSING PROTEINS 审中-公开
标题翻译：清洁蛋白质的方法
公开(公告)号：WO1991000290A1
公开(公告)日：1991-01-10
申请号：PCT/SE1990000442
申请日：1990-06-20
申请人： KABIVITRUM AB , EKETORP, Rainer
发明人： KABIVITRUM AB
IPC分类号： C07K03/12
CPC分类号： C07K14/76 , C07K1/34 , C07K16/065
摘要： The invention relates to a method of cleansing a protein from multivalent metal ions bound thereto, these ions being released from the protein by exchanging the ions with monovalent metal ions, whereafter the multivalent metal ions are removed. The release and removal of these ions is effected, in particular, by diafiltration or gel filtration processes.

4. 发明申请

WO1988008428A1 METHOD FOR PURIFYING GRANULOCYTE/MACROPHAGE COLONY STIMULATING FACTOR 审中-公开
标题翻译：净化粒细胞/巨噬细胞刺激因子的方法
公开(公告)号：WO1988008428A1
公开(公告)日：1988-11-03
申请号：PCT/US1988001228
申请日：1988-04-19
申请人： KIRIN-AMGEN, INC.
发明人： KIRIN-AMGEN, INC. , BOONE, Thomas, C.
IPC分类号： C07K03/12
CPC分类号： C07K14/535
摘要： A process for isolating and purifying GM-CSF produced from recombinant sources. The process provides for the isolation and purifying of recombinant GM-CSF produced in E. coli.

5. 发明申请

WO1994014837A1 PURIFICATION OF PROTEINACEOUS MATERIAL 审中-公开
标题翻译：蛋白质材料的纯化
公开(公告)号：WO1994014837A1
公开(公告)日：1994-07-07
申请号：PCT/US1993012395
申请日：1993-12-17
申请人： ENZON, INC.
发明人： ENZON, INC. , LEE, Lihsyng, S. , MUTHUKUMAR, Ganapathy , CZUBA, Barbara , SHORR, Robert, G., L.
IPC分类号： C07K03/12
CPC分类号： C12Y302/01045 , A61K38/00 , C12N9/2402
摘要： Methods for the purification of proteinaceous materials, such as recombinant glucocerebrosidase (GCS) expressed in an insect cell culture that had been previously infected with a baculovirus carrying GCS-encoding nucleic acid, are disclosed. According to one embodiment, the medium is first clarified by being passed through a hollowfiber cartridge, then bacteria is filtered out. The filtered media is then concentrated with a cation exchange column, followed by hydrophobic interaction chromatography (HIC) purification. In alternative embodiments, the eluate from the cation exchange column and/or the HIC column is passed through an anion exchange column for removal of residual DNA, lipids and endotoxins. The purification methods of the present invention are quicker, cheaper, and capable of handling greater quantities and greater starting purities of conditioned media than processes previously utilized. The various methods of the present invention are also capable of providing commercial quantities of purified GCS suitable for therapeutic uses. Purified forms of GCS are also disclosed.
摘要翻译：公开了在先前用携带有GCS编码核酸的杆状病毒感染的昆虫细胞培养物中表达的蛋白质物质如重组葡糖脑苷脂酶（GCS）的纯化方法。根据一个实施例，首先通过穿过中空纤维筒来澄清培养基，然后将细菌滤出。然后将过滤的介质用阳离子交换柱浓缩，随后进行疏水相互作用层析（HIC）纯化。在替代实施方案中，将来自阳离子交换柱和/或HIC柱的洗脱液通过阴离子交换柱以除去残留的DNA，脂质和内毒素。与先前使用的方法相比，本发明的纯化方法更快，更便宜并且能够处理条件培养基的更大量和更高的起始纯度。本发明的各种方法还能够提供适合于治疗用途的商业量的纯化GCS。还公开了纯化形式的GCS。

6. 发明申请

WO1994007912A1 AFFINITY PURIFICATION METHODS INVOLVING AMINO ACID MIMETICS AS ELUTION REAGENTS 审中-公开
标题翻译：涉及用作溶液试剂的氨基酸化物质的亲和纯化方法
公开(公告)号：WO1994007912A1
公开(公告)日：1994-04-14
申请号：PCT/US1993009174
申请日：1993-09-27
申请人： THE GENERAL HOSPITAL CORPORATION
发明人： THE GENERAL HOSPITAL CORPORATION , SEED, Brian
IPC分类号： C07K03/12
CPC分类号： C07K1/22 , C07K16/065
摘要： Disclosed is a method of isolating a protein from a sample, involving (i) providing a first molecule which is capable of forming an affinity complex with the protein; (ii) contacting the sample with the first molecule under conditions which allow affinity complex formation; (iii) isolating the complex; (iv) treating the complex with a second molecule, the second molecule mimicking an amino acid residue of either the protein or the first molecule which is critical to the complex formation, so that the second molecule disrupts the complex; causing the release of the protein from the complex; and (v) isolating the protein. According to one embodiment, the amino acid mimetic imidazole is used as a very gentle elution reagent to disrupt a protein A-antibody fusion protein complex, a technique which has general application for the isolation of antibodies or recombinant antibody fusion proteins.
摘要翻译：公开了一种从样品中分离蛋白质的方法，其包括（i）提供能够与蛋白质形成亲和复合物的第一分子; （ii）在允许形成亲和性复合物的条件下使样品与第一分子接触; （iii）分离复合物; （iv）用第二分子处理所述复合物，所述第二分子模拟所述蛋白质或所述第一分子的氨基酸残基对所述复合体形成至关重要，以致所述第二分子破坏所述复合物; 引起蛋白质从复合物的释放; 和（v）分离蛋白质。根据一个实施方案，氨基酸模拟咪唑用作非常温和的洗脱剂以破坏蛋白A抗体融合蛋白复合物，该技术具有分离抗体或重组抗体融合蛋白的一般应用。

7. 发明申请

WO1992011283A1 TRANSGENIC, CROSS-LINKED HEMOGLOBIN 审中-公开
标题翻译：转基因，交联HEMOGLOBIN
公开(公告)号：WO1992011283A1
公开(公告)日：1992-07-09
申请号：PCT/US1991009624
申请日：1991-12-20
申请人： THE UNIVERSITY OF ALABAMA RESEARCH FOUNDATION
发明人： THE UNIVERSITY OF ALABAMA RESEARCH FOUNDATION , TOWNES, Tim, M. , McCUNE, Steven, L.
IPC分类号： C07K03/12
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2227/105 , A01K2267/01 , A61K38/00 , C07K14/805
摘要： Transgenic, recombinantly cross-linked polymeric human hemoglobins suitable as cell-free blood substitutes have been produced. A plurality of DNA constructs have been designed for efficient expression of modified human hemoglobins in the erythrocytes of the non-human transgenic animals. Substantially pure, non-immunogenic, artificial human hemoglobins are then easily obtained from the erythroid of the transgenic animals.
摘要翻译：已经生产了适合作为无细胞血液代用品的转基因重组交联聚合人血红蛋白。已经设计了多个DNA构建体用于在非人转基因动物的红细胞中有效表达修饰的人血红蛋白。然后从转基因动物的红细胞容易地获得基本上纯的，非免疫原性的人造血红蛋白。

8. 发明申请

WO1991004267A1 A METHOD FOR THE PURIFICATION OF TRANSFORMING GROWTH FACTOR (TGF-) 审中-公开
标题翻译：变性生长因子（TGF-）的纯化方法
公开(公告)号：WO1991004267A1
公开(公告)日：1991-04-04
申请号：PCT/US1990005098
申请日：1990-09-10
申请人： CODON , PUNGOR, Erno, Jr. , NESTAAS, Eirik
发明人： CODON
IPC分类号： C07K03/12
CPC分类号： C12M47/02 , C07K14/495
摘要： Transforming growth factor β (TGF-β) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF-β and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF-β may be removed while the protein is bound to the cation exchange resin. Purified TGF-β1 compositions have a specific activity above 107 U/ml.

9. 发明申请

WO1994022903A1 SEPARATION OF PROTEINS 审中-公开
标题翻译：蛋白质分离
公开(公告)号：WO1994022903A1
公开(公告)日：1994-10-13
申请号：PCT/DK1994000132
申请日：1994-03-29
申请人： NOVO NORDISK A/S , NILSSON, Birgitte, Mahler , LAUSTSEN, Mads, Aage , PAHLE, Christine
发明人： NOVO NORDISK A/S
IPC分类号： C07K03/12
CPC分类号： C12N9/20 , C12N9/00 , Y10S435/814 , Y10S435/816
摘要： The present invention relates to a method of separating an enzyme from an aqueous solution comprising this enzyme in mixture with other proteins, and recovery of the desired enzyme on crystalline form.
摘要翻译：本发明涉及从包含该酶的水溶液与其它蛋白质的混合物中分离酶的方法，并且以结晶形式回收所需酶。

10. 发明申请

WO1994009027A1 FRACTIONATION OF POLYALKYLENE OXIDE-CONJUGATED HEMOGLOBIN SOLUTIONS 审中-公开
标题翻译：聚亚烷基氧化物结合的HEMOGLOBIN溶液的分离
公开(公告)号：WO1994009027A1
公开(公告)日：1994-04-28
申请号：PCT/US1993006972
申请日：1993-07-26
申请人： ENZON, INC.
发明人： ENZON, INC. , SHORR, Robert, G., L. , CHO, Myung-Ok , NHO, Kwang
IPC分类号： C07K03/12
CPC分类号： C07K17/06 , A61K38/00 , A61K47/60 , A61K47/6445 , C07K14/805
摘要： Hemoglobin-containing solutions containing polyalkylene oxide-conjugated hemoglobin having a molecular weight greater than about 85,000 daltons and a degree of substitution of at least five polyalkylene oxide conjugates per hemoglobin molecule are described that are not associated with hemoglobinuria in mammals. A method of simultaneously fractionating and purifying polyalkylene oxide-conjugated hemoglobins is also disclosed.
摘要翻译：描述了含有分子量大于约85,000道尔顿的聚环氧烷共轭血红蛋白和每个血红蛋白分子至少五个聚环氧烷共轭物的取代度的含血红蛋白的溶液，其与哺乳动物的血红蛋白尿不相关。还公开了同时分离和纯化聚环氧烷共轭血红蛋白的方法。

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