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1. 发明申请

WO2015194834A1 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도 审中-公开
标题翻译：包含基因片段的载体，用于增强重组蛋白表达及其使用
公开(公告)号：WO2015194834A1
公开(公告)日：2015-12-23
申请号：PCT/KR2015/006098
申请日：2015-06-16
申请人： 한국생명공학연구원
发明人： 이은교 , 이홍원 , 강신영 , 김연구 , 정준기 , 안정오 , 강승희 , 김천석 , 이혁원 , 이진겸
IPC分类号： C12N15/63 , C12Q1/68 , C12N15/85
CPC分类号： C12N15/85 , C07K14/43595 , C07K16/00 , C07K2317/14 , C12N15/63 , C12N2840/203 , C12N2840/60 , C12N2840/85 , C12Q1/68 , C12Q1/6876 , C12Q1/6897
摘要： 본 발명은 동물세포에서 재조합 단백질 발현 증진을 위한 CHO(Chinese Hamster Ovary) 세포 유래의 신규 유전자 절편 및 이의 용도에 관한 것으로, 본 발명의 유전자 절편을 포함하는 벡터를 이용하면 동물세포에서 목적 단백질의 발현이 증직되는 것을 확인하였으므로 치료용 항체 등의 생물의약품 생산에 유용하게 이용할 수 있을 것이다.
摘要翻译：本发明涉及用于增强动物细胞中重组蛋白表达的新型中国仓鼠卵巢（CHO）细胞来源的基因片段及其用途。已经发现，使用包含本发明的基因片段的载体增强了靶蛋白在动物细胞中的表达。因此，包含本发明的基因片段的载体可用于制备生物药物如治疗性抗体等。

2. 发明申请

WO00018240A1 GENE DELIVERY SYSTEM AND METHODS OF USE 审中-公开
标题翻译：基因递送系统及其使用方法
公开(公告)号：WO00018240A1
公开(公告)日：2000-04-06
申请号：PCT/US1999/023016
申请日：1999-10-01
IPC分类号： C12N7/01 , C12N15/86 , C12N15/867 , A01N63/00
CPC分类号： C12N15/86 , C12N2740/13043 , C12N2740/13045 , C12N2810/50 , C12N2810/80 , C12N2810/854 , C12N2810/859 , C12N2830/002 , C12N2830/008 , C12N2830/15 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/85
摘要： A recombinant replication competent retrovirus for gene delivery and gene therapy is provided. The recombinant retrovirus has a heterologous nucleic acid sequence, a sequence encoding a cell- or tissue-specific ligand or a sequence for transcriptional targeting, or a combination of both a cell- or tissue-specific ligand and a cell- or tissue-specific transcriptional targeting sequence.
摘要翻译：提供了用于基因递送和基因治疗的重组复制型逆转录病毒。重组逆转录病毒具有异源核酸序列，编码细胞或组织特异性配体的序列或用于转录靶向的序列，或细胞或组织特异性配体和细胞或组织特异性转录的组合目标序列。

3. 发明申请

WO2013085624A1 VECTORS HARBORING TOXIC GENES, METHODS AND USES THEREFOR 审中-公开
标题翻译：载体含有毒性基因，方法和用途
公开(公告)号：WO2013085624A1
公开(公告)日：2013-06-13
申请号：PCT/US2012/060441
申请日：2012-10-16
申请人： CHEN, Haifeng , VIROVEK, INC.
发明人： CHEN, Haifeng
IPC分类号： C12N15/31 , C12N15/113 , C12N15/79 , C12N15/64 , C12N5/10 , A61P35/00 , A61K31/7088 , A61K35/76
CPC分类号： C12N15/86 , A61K31/7088 , A61K48/00 , C07K14/21 , C07K14/34 , C12N9/22 , C12N9/2497 , C12N2710/14043 , C12N2710/14052 , C12N2750/14143 , C12N2750/14152 , C12N2799/026 , C12N2830/42 , C12N2840/005 , C12N2840/102 , C12N2840/44 , C12N2840/85
摘要： The present disclosure describes nucleic acids, and viruses comprising such nucleic acids, for growing a toxic gene in an insect cell. These nucleic acids comprise a sequence encoding a toxic polypeptide, and an intron that interrupts the sequence, whereby the intron is spliced in mammalian cells but not in insect cells. Infection of mammalian cells but not insect cells with the nucleic acids or viruses can lead to expression of toxic levels of the toxic polypeptide in mammalian cells but not in insect cells. Viruses, such as an AAV or a baculovirus comprising a nucleic acid can be grown in insect cell lines in vitro and can be administered to a subject in need of therapy, such as a subject in need of cancer therapy.
摘要翻译：本公开描述了用于在昆虫细胞中生长毒性基因的核酸和包含此类核酸的病毒。这些核酸包含编码毒性多肽的序列和中断该序列的内含子，由此内含子在哺乳动物细胞中剪接而不在昆虫细胞中剪接。哺乳动物细胞而不是昆虫细胞与核酸或病毒的感染可导致在哺乳动物细胞中毒性水平的毒性水平的表达，但不能在昆虫细胞中表达。病毒如AAV或包含核酸的杆状病毒可以在昆虫细胞系中体外生长，并且可以施用于需要治疗的受试者，例如需要癌症治疗的受试者。

4. 发明申请

WO2005072129A9 PRODUCTION OF HOST CELLS CONTAINING MUTIPLE INTEGRATING VECTORS BY SERIAL TRANSDUCTION 审中-公开
标题翻译：通过串行传输产生包含多个积分矢量的主体细胞
公开(公告)号：WO2005072129A9
公开(公告)日：2009-09-17
申请号：PCT/US2005001165
申请日：2005-01-14
申请人： GALA DESIGN INC , BLECK GREGORY T , BREMEL ROBERT D , MILLER LINDA U
发明人： BLECK GREGORY T , BREMEL ROBERT D , MILLER LINDA U
IPC分类号： C12N15/867 , C07K14/02 , C07K14/76 , C07K16/12 , C07K16/30 , C12N5/10 , C12N7/04 , C12N15/82 , C12N15/85 , C12P21/02
CPC分类号： C12P21/02 , C07K14/005 , C07K14/76 , C07K16/1228 , C07K16/1282 , C07K16/30 , C07K16/3007 , C07K16/3046 , C07K2319/00 , C07K2319/02 , C12N7/00 , C12N15/8203 , C12N15/8216 , C12N15/8241 , C12N15/85 , C12N15/86 , C12N2510/02 , C12N2740/13022 , C12N2740/13023 , C12N2740/13043 , C12N2740/13051 , C12N2760/16122 , C12N2830/00 , C12N2830/008 , C12N2830/15 , C12N2830/48 , C12N2830/85 , C12N2840/203 , C12N2840/85
摘要： The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.
摘要翻译：本发明涉及在宿主细胞中产生蛋白质，更具体地涉及含有包含外源基因的整合载体的多个整合拷贝的宿主细胞和通过连续转导或转染制备这种宿主细胞的方法。本发明还提供了使用这种载体在宿主细胞中表达增加的蛋白质水平的方法。

5. 发明申请

WO2009036280A1 LONG-TERM IN VIVO TRANSGENE EXPRESSION 审中-公开
标题翻译： VIVO TRANSGENE EXPRESSION的长期使用
公开(公告)号：WO2009036280A1
公开(公告)日：2009-03-19
申请号：PCT/US2008/076177
申请日：2008-09-12
申请人： COPERNICUS THERAPEUTICS, INC. , COOPER, Mark J. , PADEGIMAS, Linas
发明人： COOPER, Mark J. , PADEGIMAS, Linas
IPC分类号： C12N15/85
CPC分类号： C07K14/4712 , A61K48/0066 , C12N15/85 , C12N2799/04 , C12N2830/42 , C12N2830/50 , C12N2830/85 , C12N2840/00 , C12N2840/85
摘要： Efficient and prolonged hCFTR expression is one of the major obstacles for cystic fibrosis lung therapy. hCFTR mRNA expression levels depend on eukaryotic expression cassette components, prokaryotic backbone elements, and the gene transfer method may also influence transcriptional silencing mechanisms. A codon-optimized and CpG-reduced human CFTR gene (CO-CFTR) was made. Various vector modifications were tested to facilitate extended duration of CO-CFTR expression. Insertion of an extended 3'BGH transcribed sequence (712 bp) in an inverted orientation produced prolonged expression of CO-CFTR expression at biologically relevant levels. Further studies revealed that prolonged CO-CFTR expression is dependant on the orientation of the extended BGH 3' BGH transcribed sequence and its transcription, is not specific to the UbC promoter, and is less dependent on other vector backbone elements.
摘要翻译：有效和长期的hCFTR表达是囊性纤维化肺部治疗的主要障碍之一。 hCFTR mRNA表达水平取决于真核表达盒组分，原核骨架元素，基因转移方法也可能影响转录沉默机制。制备了密码子优化和CpG还原的人CFTR基因（CO-CFTR）。测试各种载体修饰以促进CO-CFTR表达的延长的持续时间。以倒置方向插入延伸的3'BGH转录序列（712bp）产生CO-CFTR表达在生物相关水平上的延长表达。进一步的研究表明，延长的CO-CFTR表达依赖于扩展的BGH 3'BGH转录序列的方向及其转录，对UbC启动子不是特异性的，并且较少依赖于其它载体骨架元素。

6. 发明申请

WO1994024870A1 RETROVIRAL VECTORS CAPABLE OF EXPRESSING MULTIMERIC PROTEINS FROM MULTIPLE TRANSLATIONAL INITIATION SITES 审中-公开
标题翻译：能够从多个翻译启动站点表达多种蛋白质的转染载体
公开(公告)号：WO1994024870A1
公开(公告)日：1994-11-10
申请号：PCT/US1994000650
申请日：1994-01-17
申请人： BIOTRANSPLANT, INC. , THE GENERAL HOSPITAL CORPORATION , LE GUERN, Christian, A. , SEED, Brian , BANERJEE, Papia, T.
发明人： BIOTRANSPLANT, INC. , THE GENERAL HOSPITAL CORPORATION
IPC分类号： A01N63/00
CPC分类号： C12N15/86 , C07K14/70539 , C12N15/79 , C12N2740/13043 , C12N2800/108 , C12N2840/203 , C12N2840/206 , C12N2840/44 , C12N2840/85
摘要： This invention relates to retroviral vectors that express multiple polypeptide subunits of a eukaryotic protein from a single polycistronic mRNA and the proteins produced from these vectors. The expressed proteins are particularly useful for inducing transplantation tolerance and the vectors are useful as agents for mediating gene transfer into eukaryotic cells.
摘要翻译：本发明涉及从单个多顺反子mRNA和由这些载体产生的蛋白质表达真核蛋白的多个多肽亚基的逆转录病毒载体。表达的蛋白质对于诱导移植耐受特别有用，并且载体可用作介导基因转移到真核细胞中的试剂。

7. 发明申请

WO2013167265A1 NUCLEIC ACID CONSTRUCT AND USE OF THE SAME 审中-公开
标题翻译：核酸结构及其用途
公开(公告)号：WO2013167265A1
公开(公告)日：2013-11-14
申请号：PCT/EP2013/001356
申请日：2013-05-07
申请人： KOCHANEK, Stefan
发明人： KOCHANEK, Stefan , LUCAS, Tanja , KUEPPERS, Claudia
IPC分类号： C12N15/861 , C12N5/10
CPC分类号： C12N15/85 , C12N15/86 , C12N15/861 , C12N2710/10343 , C12N2840/44 , C12N2840/85
摘要： The present invention is related to a nucleic acid construct comprising - an expression unit for the expression of EIB, wherein the expression unit comprises a promoter, a nucleotide sequence coding for EIB, and a 3'UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for EIB, wherein the 3'UTR comprises 30 or less than 30 Exonic Enhancer Elements (ESEs), preferably 20 or less than 20 Exonic Enhancer Elements (ESEs), and wherein the 3' UTR is a non-viral 3' UTR.
摘要翻译：本发明涉及核酸构建体，其包含 - 用于表达EIB的表达单元，其中所述表达单元包含启动子，编码EIB的核苷酸序列和3'UTR，其中所述启动子可操作地连接到编码EIB的核苷酸序列，其中3'UTR包含30个或少于30个Exonic Enhancer Elements（ESE），优选20个或少于20个Exonic Enhancer Elements（ESE），并且其中3'UTR是非病毒3' UTR。

8. 发明申请

WO2013063348A1 GENETIC ELEMENT THAT ENHANCES PROTEIN TRANSLATION 审中-公开
标题翻译：提高蛋白质翻译的遗传元素
公开(公告)号：WO2013063348A1
公开(公告)日：2013-05-02
申请号：PCT/US2012/062045
申请日：2012-10-26
申请人： ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA, ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY
发明人： CHAPUT, John , JACOBS, Bertram , WELLENSIEK, Brian , FLORES, Julia
IPC分类号： C12N15/113 , C12N15/85
CPC分类号： C12N15/113 , C12N15/67 , C12N15/85 , C12N2830/30 , C12N2830/85 , C12N2840/105 , C12N2840/85
摘要： The present invention provides isolated polynucleotides that can serve as translation enhancing elements and their use in protein expression reagents and methods.
摘要翻译：本发明提供可用作翻译增强元件的分离的多核苷酸及其在蛋白质表达试剂和方法中的用途。

9. 发明申请

WO2011066454A1 COMPOSITIONS, METHODS AND USES FOR EXPRESSION OF ENTEROBACTERIUM-ASSOCIATED PEPTIDES 审中-公开
标题翻译：组合物，用于表达肠壁菌相关肽的方法和用途
公开(公告)号：WO2011066454A1
公开(公告)日：2011-06-03
申请号：PCT/US2010/058094
申请日：2010-11-24
申请人： INVIRAGEN, INC. , STINCHCOMB, Dan, T. , OSORIO, Jorge, E. , POWELL, Timothy, D. , BREWOO, Joseph, N.
发明人： STINCHCOMB, Dan, T. , OSORIO, Jorge, E. , POWELL, Timothy, D. , BREWOO, Joseph, N.
IPC分类号： A61K39/02
CPC分类号： A61K39/025 , A61K39/0291 , A61K2039/5256 , A61K2039/53 , C07K14/24 , C12N7/00 , C12N15/86 , C12N2710/24134 , C12N2710/24141 , C12N2710/24143 , C12N2710/24171 , C12N2840/002 , C12N2840/85 , Y02A50/407
摘要： Embodiments of the present invention generally disclose methods, compositions and uses for generating and expressing enterobacterial-associated peptides. In some embodiments, enterobacterial-associated peptides include, but are not limited to plague-associated peptides. In certain embodiments, methods generally relate to making and using compositions of constructs including, but not limited to, attenuated or modified vaccinia virus vectors expressing enterobacterial-associated peptides. In other embodiments, vaccine compositions are reported of use in a subject.
摘要翻译：本发明的实施方案通常公开了用于产生和表达肠细菌相关肽的方法，组合物和用途。在一些实施方案中，肠细菌相关肽包括但不限于瘟疫相关肽。在某些实施方案中，方法通常涉及制备和使用构建体的组合物，包括但不限于表达肠细菌相关肽的减毒或修饰的痘苗病毒载体。在其它实施方案中，报告了在受试者中使用的疫苗组合物。

10. 发明申请

WO2008022253A3 AN UNCONVENTIONAL ANTIGEN TRANSLATED BY A NOVEL INTERNAL RIBOSOME ENTRY SITE ELICITS ANTITUMOR HUMORAL IMMUNE REACTIONS 审中-公开
标题翻译：一个新的内部RIBOSOME入口站点翻译的非传统抗原引发抗肿瘤免疫反应
公开(公告)号：WO2008022253A3
公开(公告)日：2008-10-30
申请号：PCT/US2007076088
申请日：2007-08-16
申请人： UNIV TEMPLE , YANG XIAO-FENG
发明人： YANG XIAO-FENG
IPC分类号： C07H21/02 , A01N43/04 , A01N63/00 , A01N65/00 , A61K38/00 , C07H21/04 , C12N5/00
CPC分类号： C07K14/4702 , A61K39/00 , A61K39/0011 , A61K2039/53 , C07K14/4748 , C12N2840/002 , C12N2840/105 , C12N2840/203 , C12N2840/85
摘要： The invention provides a novel antigen, MPD6, which belongs to the group of cryptic antigens without conventional genomic structure and is encoded by a cryptic open reading frame located in the 3'untranslated region (3'UTR) of myotrophin mRNA. MPD6 elicits IgG antibody responses in a subset of PV patients, as well as patients with chronic myelogenous leukemia and prostate cancer. The translation of MPD6 was mediated by a novel internal ribosome entry site (IRES) upstream of the MPD6 reading frame. Furthermore, the MPD6-IRES mediated translation, but not myotrophin-MPD6 transcription, was significantly upregulated in response to IFN-a stimulation. These findings demonstrate that a novel IRES-mediated mechanism is responsible for the translation of unconventional self-antigen MPD6 in responsive to IFN-a stimulation. The eliciting anti-tumor immune response against unconventional antigen MPD6 in patients with myeloproliferative diseases indicates MPD6 as a target of novel immunotherapy.
摘要翻译：本发明提供了一种新型抗原MPD6，其属于无常规基因组结构并且由位于肌营养蛋白mRNA的3'非翻译区（3'UTR）中的隐蔽性开放阅读框编码的神秘抗原组。 MPD6在一部分PV患者以及慢性粒细胞白血病和前列腺癌患者中引发IgG抗体应答。 MPD6的翻译由MPD6阅读框上游的新型内部核糖体进入位点（IRES）介导。此外，MPD6-IRES介导的翻译，但不是肌营养不良MPD6转录，响应IFN-α刺激而显着上调。这些发现表明，一种新的IRES介导的机制负责翻译非常规自身抗原MPD6对IFN-α刺激的反应。在骨髓增殖性疾病患者中针对非常规抗原MPD6引发的抗肿瘤免疫应答表明MPD6作为新型免疫疗法的靶标。

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